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The map label for this gene is ghrA [H]

Identifier: 161526123

GI number: 161526123

Start: 3235395

End: 3236336

Strand: Direct

Name: ghrA [H]

Synonym: Bmul_2954

Alternate gene names: 161526123

Gene position: 3235395-3236336 (Clockwise)

Preceding gene: 161526122

Following gene: 161526124

Centisome position: 93.82

GC content: 71.76

Gene sequence:

>942_bases
ATGAAGATCCTGTTCCATTCGCCGCACCAGGACGCCGCCGCCTGGCGCGACGCGTTCGCGCGCGTGCTGCCCGAGGCCGA
ACTACGCGCGTGGCAACCGGGCGATACGGCCGCGGCCGACTATGCGCTCGTATGGCGCGCACCGCCCGCGTTCTTCGCGC
CGCGCGACGGACTGCGCGCGATCTTCAATCTCGGCGCGGGCGTCGACGCGCTGCTCGCGCTCGAACGCGCCCACCCCGGC
ACGCTGCCGCGGCACGTGCCGCTCGTGCGGCTCGAAGATTCGGGCATGGCGCAGCAGATGATCGAATACGTGACGCACGC
CGTGCTGCGCTACCTGCGCCGTTTCGACGAATACGACGCCTTGCAGCGCGAGCGTCGCTGGCAGGTGCTCGACGCGCATC
CGCGCGCGAGCTTCGTCGTCGCGGTGCTCGGCCTCGGCGTGCTCGGCACGCAGGTCGCACGTGCGCTCGCGGCGCTCGGC
CTGCCGGTGCGCGGCTACAGCCGCAGCGCGAAACGCATCGACGGCATCGAGACATTCGCCGGCGACGACGCGCTCGACGC
CTGCCTCGACGGCGCGAAGGTGCTCGTGAATCTGCTGCCGAGCACGCCGGCCACCGACGGCCTGCTGTGCGCACGCACGT
TCGCGCGGCTTGCGCCGGGCGCCTATCTGATCAACGTCGCGCGCGGCGCGCATCTTGTCGAAGCCGATCTGCTCGACGCG
CTCGCGAGCGGCCGGATCGCCGCGGCGACGCTCGACGTGTTCCAGCACGAGCCGCTGCCCGCCGATCATCCGTTCTGGCA
GACGCCGCGCATCACGATCACGCCGCACAGCTCGGCCGAGACGCTGCGCGACGAAGCCGTCGAGCAGATCGCCGCGAAGA
TCCGCGCCTTCGAGCGCGGCGAGCCGGTCGGCGGCATCGTCGACTACGCGCGCGGCTACTGA

Upstream 100 bases:

>100_bases
GTCGGCGAGCAGGCGACGATGTTCTTCCTCGATCCGTGCGGCAATGCGCTCGAATTCAAGGCATTCGCCGACATCGGCCA
GCTGTTCGCGAAGTGAGCGC

Downstream 100 bases:

>100_bases
CGCCCGCCATAAAGCAACCAGGAGACAACCATGACGTTCCCCACCGCCGTCAAGATCGTCGAAGTCGGCCCGCGCGACGG
GCTGCAGAACGAAAAGACCT

Product: D-isomer specific 2-hydroxyacid dehydrogenase

Products: NA

Alternate protein names: 2-ketoacid reductase [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MKILFHSPHQDAAAWRDAFARVLPEAELRAWQPGDTAAADYALVWRAPPAFFAPRDGLRAIFNLGAGVDALLALERAHPG
TLPRHVPLVRLEDSGMAQQMIEYVTHAVLRYLRRFDEYDALQRERRWQVLDAHPRASFVVAVLGLGVLGTQVARALAALG
LPVRGYSRSAKRIDGIETFAGDDALDACLDGAKVLVNLLPSTPATDGLLCARTFARLAPGAYLINVARGAHLVEADLLDA
LASGRIAAATLDVFQHEPLPADHPFWQTPRITITPHSSAETLRDEAVEQIAAKIRAFERGEPVGGIVDYARGY

Sequences:

>Translated_313_residues
MKILFHSPHQDAAAWRDAFARVLPEAELRAWQPGDTAAADYALVWRAPPAFFAPRDGLRAIFNLGAGVDALLALERAHPG
TLPRHVPLVRLEDSGMAQQMIEYVTHAVLRYLRRFDEYDALQRERRWQVLDAHPRASFVVAVLGLGVLGTQVARALAALG
LPVRGYSRSAKRIDGIETFAGDDALDACLDGAKVLVNLLPSTPATDGLLCARTFARLAPGAYLINVARGAHLVEADLLDA
LASGRIAAATLDVFQHEPLPADHPFWQTPRITITPHSSAETLRDEAVEQIAAKIRAFERGEPVGGIVDYARGY
>Mature_313_residues
MKILFHSPHQDAAAWRDAFARVLPEAELRAWQPGDTAAADYALVWRAPPAFFAPRDGLRAIFNLGAGVDALLALERAHPG
TLPRHVPLVRLEDSGMAQQMIEYVTHAVLRYLRRFDEYDALQRERRWQVLDAHPRASFVVAVLGLGVLGTQVARALAALG
LPVRGYSRSAKRIDGIETFAGDDALDACLDGAKVLVNLLPSTPATDGLLCARTFARLAPGAYLINVARGAHLVEADLLDA
LASGRIAAATLDVFQHEPLPADHPFWQTPRITITPHSSAETLRDEAVEQIAAKIRAFERGEPVGGIVDYARGY

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=258, Percent_Identity=29.4573643410853, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI23308577, Length=267, Percent_Identity=27.3408239700375, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI87081824, Length=313, Percent_Identity=48.8817891373802, Blast_Score=302, Evalue=2e-83,
Organism=Escherichia coli, GI87082289, Length=157, Percent_Identity=31.2101910828025, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1789279, Length=159, Percent_Identity=30.8176100628931, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17532191, Length=171, Percent_Identity=30.9941520467836, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6322116, Length=182, Percent_Identity=29.1208791208791, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320925, Length=182, Percent_Identity=29.1208791208791, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24585516, Length=146, Percent_Identity=30.8219178082192, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19921140, Length=161, Percent_Identity=32.2981366459627, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI28574286, Length=147, Percent_Identity=31.9727891156463, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI28571528, Length=156, Percent_Identity=30.7692307692308, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 34099; Mature: 34099

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILFHSPHQDAAAWRDAFARVLPEAELRAWQPGDTAAADYALVWRAPPAFFAPRDGLRA
CEEEECCCCHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHH
IFNLGAGVDALLALERAHPGTLPRHVPLVRLEDSGMAQQMIEYVTHAVLRYLRRFDEYDA
HHHCCCCHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LQRERRWQVLDAHPRASFVVAVLGLGVLGTQVARALAALGLPVRGYSRSAKRIDGIETFA
HHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHC
GDDALDACLDGAKVLVNLLPSTPATDGLLCARTFARLAPGAYLINVARGAHLVEADLLDA
CCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEHHCCCHHHHHHHHHH
LASGRIAAATLDVFQHEPLPADHPFWQTPRITITPHSSAETLRDEAVEQIAAKIRAFERG
HHCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCC
EPVGGIVDYARGY
CCCCHHHHHHCCC
>Mature Secondary Structure
MKILFHSPHQDAAAWRDAFARVLPEAELRAWQPGDTAAADYALVWRAPPAFFAPRDGLRA
CEEEECCCCHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHH
IFNLGAGVDALLALERAHPGTLPRHVPLVRLEDSGMAQQMIEYVTHAVLRYLRRFDEYDA
HHHCCCCHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LQRERRWQVLDAHPRASFVVAVLGLGVLGTQVARALAALGLPVRGYSRSAKRIDGIETFA
HHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHC
GDDALDACLDGAKVLVNLLPSTPATDGLLCARTFARLAPGAYLINVARGAHLVEADLLDA
CCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEHHCCCHHHHHHHHHH
LASGRIAAATLDVFQHEPLPADHPFWQTPRITITPHSSAETLRDEAVEQIAAKIRAFERG
HHCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCC
EPVGGIVDYARGY
CCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA