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The map label for this gene is gcvA [H]

Identifier: 161526115

GI number: 161526115

Start: 3225218

End: 3226138

Strand: Direct

Name: gcvA [H]

Synonym: Bmul_2946

Alternate gene names: 161526115

Gene position: 3225218-3226138 (Clockwise)

Preceding gene: 161526112

Following gene: 161526119

Centisome position: 93.53

GC content: 73.29

Gene sequence:

>921_bases
ATGCGACGCATCCATCTCCCTCCGCTGCAGACGTTACGTGCGTTCGAGGCCGCCGTCCGGCTGCAAAGCTTCACGCGCGC
AGCCGACGAACTCGCGCTCACGCAAGGCGCCGTGAGCCAGCACATCCGCGCGCTCGAGGCGCAGCTCGGCTATCCGCTCT
TCACGCGCGAGCGCGGCGGCGCGACGCCGTCTCACGCCGCGCATGCGCTCGCGCTGCAAGTGCGCCAGGGGCTCAGCGTG
CTCGAGCGCGCGTTCGAGCCGACGCATGCGGTGCGTGCCCGGCCGCGCGACGGCGACGTCACGCTCGACGTCAGCGTGCT
GCCGAGCGTGGCCGAGCGCTGGCTCGCGCCGCGGCTGCCGCGCTTCGCGGCCGCGCATCCGCAGATCTCGATCGTGCTGC
ATCCGGACACCGCGCTCGCGCCGCTTCGCAAGCGCGACCGCATCGACGTCGCGCTGCGCTACGGGCCCGGCACGTGGCCG
GGCGTCGTCGCCGAGAAGCTGATGAACGAGACGGTGTTTCCGGTCGCGAGCCCCGCGTACCGCAACCGCGACGGCATCGC
GCCGCGCGCGCCGGCGGACCTCGTGCGCGCGACGCTGCTGCGCCATCCCGCGCAGCCGTGGGAACCGTGGTTTCAGGCCG
CGCGGCTCGATCTGACCGAATCGGCGCGCGCGCCGCGCTTCGCGGACGCGAACGCGCTGATCGACGCGGTGCTGAAAGGA
CGCGGCGTCGCGCTCGCGCGCCGCTCGCTGATCGAACGCGAACTTGCGAGCGGCGCGCTCGTGCGCGTATCGACGGTGCG
CATCACCGACGTCTATGCGCATTACGTCGTGTGGCGCCCCGGCCATCCGCGCGAAGCGGCGATCCGCACCTGGCTCGACT
GGCTGCGCAGCGAAGTGCGGCGCCGCACGCCGCGACGCTGA

Upstream 100 bases:

>100_bases
CCGGCGGGCCTGTGAAAGACGAGCACGAGTGTAGGAATGCCGCGCCCGCTGCCGCAAACGATGAATTGTCGTGCTCGGAC
ATTAGTGTTACTAATGCGCA

Downstream 100 bases:

>100_bases
CGCGCGCCTCAGGTGGACGCGGCCATCACCTTGTCGAGCGTGATCGGCAGATCGCGCACGCGCACGCCGGTCGCGTGATA
AACCGCATTCGCGATCGCGG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MRRIHLPPLQTLRAFEAAVRLQSFTRAADELALTQGAVSQHIRALEAQLGYPLFTRERGGATPSHAAHALALQVRQGLSV
LERAFEPTHAVRARPRDGDVTLDVSVLPSVAERWLAPRLPRFAAAHPQISIVLHPDTALAPLRKRDRIDVALRYGPGTWP
GVVAEKLMNETVFPVASPAYRNRDGIAPRAPADLVRATLLRHPAQPWEPWFQAARLDLTESARAPRFADANALIDAVLKG
RGVALARRSLIERELASGALVRVSTVRITDVYAHYVVWRPGHPREAAIRTWLDWLRSEVRRRTPRR

Sequences:

>Translated_306_residues
MRRIHLPPLQTLRAFEAAVRLQSFTRAADELALTQGAVSQHIRALEAQLGYPLFTRERGGATPSHAAHALALQVRQGLSV
LERAFEPTHAVRARPRDGDVTLDVSVLPSVAERWLAPRLPRFAAAHPQISIVLHPDTALAPLRKRDRIDVALRYGPGTWP
GVVAEKLMNETVFPVASPAYRNRDGIAPRAPADLVRATLLRHPAQPWEPWFQAARLDLTESARAPRFADANALIDAVLKG
RGVALARRSLIERELASGALVRVSTVRITDVYAHYVVWRPGHPREAAIRTWLDWLRSEVRRRTPRR
>Mature_306_residues
MRRIHLPPLQTLRAFEAAVRLQSFTRAADELALTQGAVSQHIRALEAQLGYPLFTRERGGATPSHAAHALALQVRQGLSV
LERAFEPTHAVRARPRDGDVTLDVSVLPSVAERWLAPRLPRFAAAHPQISIVLHPDTALAPLRKRDRIDVALRYGPGTWP
GVVAEKLMNETVFPVASPAYRNRDGIAPRAPADLVRATLLRHPAQPWEPWFQAARLDLTESARAPRFADANALIDAVLKG
RGVALARRSLIERELASGALVRVSTVRITDVYAHYVVWRPGHPREAAIRTWLDWLRSEVRRRTPRR

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=296, Percent_Identity=35.472972972973, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1786448, Length=307, Percent_Identity=30.9446254071661, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1788706, Length=300, Percent_Identity=27.6666666666667, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI145693193, Length=272, Percent_Identity=30.8823529411765, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787128, Length=305, Percent_Identity=24.2622950819672, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1786401, Length=294, Percent_Identity=25.8503401360544, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787589, Length=296, Percent_Identity=25.3378378378378, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34180; Mature: 34180

Theoretical pI: Translated: 11.88; Mature: 11.88

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRIHLPPLQTLRAFEAAVRLQSFTRAADELALTQGAVSQHIRALEAQLGYPLFTRERGG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCC
ATPSHAAHALALQVRQGLSVLERAFEPTHAVRARPRDGDVTLDVSVLPSVAERWLAPRLP
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCC
RFAAAHPQISIVLHPDTALAPLRKRDRIDVALRYGPGTWPGVVAEKLMNETVFPVASPAY
HHHCCCCCEEEEECCCHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCC
RNRDGIAPRAPADLVRATLLRHPAQPWEPWFQAARLDLTESARAPRFADANALIDAVLKG
CCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHC
RGVALARRSLIERELASGALVRVSTVRITDVYAHYVVWRPGHPREAAIRTWLDWLRSEVR
CCHHHHHHHHHHHHHHCCCEEEEEEEEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHH
RRTPRR
HCCCCC
>Mature Secondary Structure
MRRIHLPPLQTLRAFEAAVRLQSFTRAADELALTQGAVSQHIRALEAQLGYPLFTRERGG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCC
ATPSHAAHALALQVRQGLSVLERAFEPTHAVRARPRDGDVTLDVSVLPSVAERWLAPRLP
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCC
RFAAAHPQISIVLHPDTALAPLRKRDRIDVALRYGPGTWPGVVAEKLMNETVFPVASPAY
HHHCCCCCEEEEECCCHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCC
RNRDGIAPRAPADLVRATLLRHPAQPWEPWFQAARLDLTESARAPRFADANALIDAVLKG
CCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHC
RGVALARRSLIERELASGALVRVSTVRITDVYAHYVVWRPGHPREAAIRTWLDWLRSEVR
CCHHHHHHHHHHHHHHCCCEEEEEEEEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHH
RRTPRR
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]