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The map label for this gene is murD [H]

Identifier: 161526007

GI number: 161526007

Start: 3092078

End: 3093589

Strand: Reverse

Name: murD [H]

Synonym: Bmul_2838

Alternate gene names: 161526007

Gene position: 3093589-3092078 (Counterclockwise)

Preceding gene: 161526008

Following gene: 161526006

Centisome position: 89.71

GC content: 71.1

Gene sequence:

>1512_bases
ATGTTTGGAGATCGGCAACGGCCGATGGTGCTCGTGCTGGGGCTCGGCGAATCGGGCCTCGCGATCGCGCGGTGGTGCGC
GAGGCACGGGTGCCGGCTGCGCATTGCCGATACCCGCGAGGCGCCGCCGAACCTTGCCGCACTGCGCGCTGAAGGCATCG
ACGCGGAATTCGTCGGCGGGCCGTTCGGGCCCGCGCTGCTCGACGGCGGCGTCGAGATCGTCGGGTTGAGCCCCGGCCTG
TCGCCGCTCGAGCCCGCGCTCGCGACGCTGCTCGCGGCCGCGAACGAGCGCGGCGTCGCCGTATGGGGCGAACTCGAATT
CTTCGCACAGGCGTTGCGCGCACTCGGCACGAGCGGCTATCAGCCGAAGGTGCTCGCGATCACGGGCACGAACGGCAAGA
CGACGACGACGAGCCTCACGGGCCTGCTGTGCCAGCGCGCGGGCAAGAAGGTCGCGGTGGCAGGCAACATCAGCCCGGCG
ATGCTCGACCGGCTCGCGGCCGCCATCGACGACACGGCGCTGCCCGACGTCTGGGTGCTCGAACTGTCGAGCTTCCAGCT
CGAAACCGCGCGCACGTTCGCACCCGATGCGGCCGCGATCCTCAACATCACGCAGGATCATCTCGACTGGCACGGCAGCT
TCGACGCGTACGCGGCCGCGAAGGGCCGGATTTTCGGCGCGACGACGACGCGCGTGCTGAACCGCGACGATCCGGTCGTG
ATGAAGTTCGCACCGGCGGCCGGCGCGGCCGACGCGCCGCGCACGATCACGTTCGGCCTGAACGAGCCGACGCAGGACGG
CGATTATGGGCTCTCGCGCGACAACGGCATCGCATGGCTCGTCGAAGCCATCGATCGCGACGCACCGGACGAAACGGCGC
CGACGACGCGCCGTCGCAAGCGCGACGCGCATACGCCCGACATCGCACACAAGCGGCTGATGCCGGCCGACGCGCTGCGC
ATTCGCGGGCTGCACAACGCGGCGAACGCGCTGGCCGCCTTCGCGCTCGCGCGCGCGATCGATCTGCCGGCCGCGCCGCT
GCTGCACGCGCTGCGCGAGTACCGCGGCGAAGCGCATCGCGTCGAAGTGATCGCGACGATCGACGACGTCGACTACGTCG
ACGACAGCAAGGGCACGAACGTCGGCGCGACGGTCGCCGCACTCGACGGGCTCGCGCAGAAGACCGTGCTGATCGCCGGC
GGCGACGGCAAGGGCCAGGATTTCGCGCCGCTCGTCGCGCCGGTCGCGCGCTGGTGCCGTGCGGTGATGCTGATCGGCCG
CGATGCGCCGGCGATCCGCGACACGCTCGCGGAAACCGGCGTGCCGCTCGTCGAGCATCCGACGCTCGAAGCGGCCGTGC
ACGCGGCGGCGGCGCTCGCCGAGCCCGGCGACGCGGTGCTGCTGTCGCCCGCATGCGCGAGCCTCGACATGTTCCGTAAC
TATGCCCACCGGGCGGACGTGTTCCGTGCGGCGGTCGACGAACTCGCCATCGACAAAGGAGCGACGCCATGA

Upstream 100 bases:

>100_bases
GTGGTGGTGCGTTTCTGGATCATCACGTTGATGCTGTGCCTGTTCGGTCTGTCCACCCTCAAGTTGCGGTAAAGGAAAGG
TAACAAGGATGTCTGGCGAG

Downstream 100 bases:

>100_bases
GCTGGTCCGATCGCCTCGTCTCCCGTTTCAACGACCGGCGCGATGCCGGCGGCAATGCCGCGGGCGGCCGCGTCGCGTCG
GCCACGCGCGCGGCGACCGG

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MFGDRQRPMVLVLGLGESGLAIARWCARHGCRLRIADTREAPPNLAALRAEGIDAEFVGGPFGPALLDGGVEIVGLSPGL
SPLEPALATLLAAANERGVAVWGELEFFAQALRALGTSGYQPKVLAITGTNGKTTTTSLTGLLCQRAGKKVAVAGNISPA
MLDRLAAAIDDTALPDVWVLELSSFQLETARTFAPDAAAILNITQDHLDWHGSFDAYAAAKGRIFGATTTRVLNRDDPVV
MKFAPAAGAADAPRTITFGLNEPTQDGDYGLSRDNGIAWLVEAIDRDAPDETAPTTRRRKRDAHTPDIAHKRLMPADALR
IRGLHNAANALAAFALARAIDLPAAPLLHALREYRGEAHRVEVIATIDDVDYVDDSKGTNVGATVAALDGLAQKTVLIAG
GDGKGQDFAPLVAPVARWCRAVMLIGRDAPAIRDTLAETGVPLVEHPTLEAAVHAAAALAEPGDAVLLSPACASLDMFRN
YAHRADVFRAAVDELAIDKGATP

Sequences:

>Translated_503_residues
MFGDRQRPMVLVLGLGESGLAIARWCARHGCRLRIADTREAPPNLAALRAEGIDAEFVGGPFGPALLDGGVEIVGLSPGL
SPLEPALATLLAAANERGVAVWGELEFFAQALRALGTSGYQPKVLAITGTNGKTTTTSLTGLLCQRAGKKVAVAGNISPA
MLDRLAAAIDDTALPDVWVLELSSFQLETARTFAPDAAAILNITQDHLDWHGSFDAYAAAKGRIFGATTTRVLNRDDPVV
MKFAPAAGAADAPRTITFGLNEPTQDGDYGLSRDNGIAWLVEAIDRDAPDETAPTTRRRKRDAHTPDIAHKRLMPADALR
IRGLHNAANALAAFALARAIDLPAAPLLHALREYRGEAHRVEVIATIDDVDYVDDSKGTNVGATVAALDGLAQKTVLIAG
GDGKGQDFAPLVAPVARWCRAVMLIGRDAPAIRDTLAETGVPLVEHPTLEAAVHAAAALAEPGDAVLLSPACASLDMFRN
YAHRADVFRAAVDELAIDKGATP
>Mature_503_residues
MFGDRQRPMVLVLGLGESGLAIARWCARHGCRLRIADTREAPPNLAALRAEGIDAEFVGGPFGPALLDGGVEIVGLSPGL
SPLEPALATLLAAANERGVAVWGELEFFAQALRALGTSGYQPKVLAITGTNGKTTTTSLTGLLCQRAGKKVAVAGNISPA
MLDRLAAAIDDTALPDVWVLELSSFQLETARTFAPDAAAILNITQDHLDWHGSFDAYAAAKGRIFGATTTRVLNRDDPVV
MKFAPAAGAADAPRTITFGLNEPTQDGDYGLSRDNGIAWLVEAIDRDAPDETAPTTRRRKRDAHTPDIAHKRLMPADALR
IRGLHNAANALAAFALARAIDLPAAPLLHALREYRGEAHRVEVIATIDDVDYVDDSKGTNVGATVAALDGLAQKTVLIAG
GDGKGQDFAPLVAPVARWCRAVMLIGRDAPAIRDTLAETGVPLVEHPTLEAAVHAAAALAEPGDAVLLSPACASLDMFRN
YAHRADVFRAAVDELAIDKGATP

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=503, Percent_Identity=35.9840954274354, Blast_Score=223, Evalue=2e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 52892; Mature: 52892

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGDRQRPMVLVLGLGESGLAIARWCARHGCRLRIADTREAPPNLAALRAEGIDAEFVGG
CCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEECCCCCCCCCCEEEEECCCCCEECCC
PFGPALLDGGVEIVGLSPGLSPLEPALATLLAAANERGVAVWGELEFFAQALRALGTSGY
CCCHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHCCCCC
QPKVLAITGTNGKTTTTSLTGLLCQRAGKKVAVAGNISPAMLDRLAAAIDDTALPDVWVL
CCEEEEEECCCCCEEHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCCCCEEEE
ELSSFQLETARTFAPDAAAILNITQDHLDWHGSFDAYAAAKGRIFGATTTRVLNRDDPVV
EECCCEEHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHCCCEEEECHHHEEECCCCCEE
MKFAPAAGAADAPRTITFGLNEPTQDGDYGLSRDNGIAWLVEAIDRDAPDETAPTTRRRK
EEECCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHH
RDAHTPDIAHKRLMPADALRIRGLHNAANALAAFALARAIDLPAAPLLHALREYRGEAHR
CCCCCCCHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEE
VEVIATIDDVDYVDDSKGTNVGATVAALDGLAQKTVLIAGGDGKGQDFAPLVAPVARWCR
EEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHH
AVMLIGRDAPAIRDTLAETGVPLVEHPTLEAAVHAAAALAEPGDAVLLSPACASLDMFRN
HHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHH
YAHRADVFRAAVDELAIDKGATP
HHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MFGDRQRPMVLVLGLGESGLAIARWCARHGCRLRIADTREAPPNLAALRAEGIDAEFVGG
CCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEECCCCCCCCCCEEEEECCCCCEECCC
PFGPALLDGGVEIVGLSPGLSPLEPALATLLAAANERGVAVWGELEFFAQALRALGTSGY
CCCHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHCCCCC
QPKVLAITGTNGKTTTTSLTGLLCQRAGKKVAVAGNISPAMLDRLAAAIDDTALPDVWVL
CCEEEEEECCCCCEEHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCCCCEEEE
ELSSFQLETARTFAPDAAAILNITQDHLDWHGSFDAYAAAKGRIFGATTTRVLNRDDPVV
EECCCEEHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHCCCEEEECHHHEEECCCCCEE
MKFAPAAGAADAPRTITFGLNEPTQDGDYGLSRDNGIAWLVEAIDRDAPDETAPTTRRRK
EEECCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHH
RDAHTPDIAHKRLMPADALRIRGLHNAANALAAFALARAIDLPAAPLLHALREYRGEAHR
CCCCCCCHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEE
VEVIATIDDVDYVDDSKGTNVGATVAALDGLAQKTVLIAGGDGKGQDFAPLVAPVARWCR
EEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHH
AVMLIGRDAPAIRDTLAETGVPLVEHPTLEAAVHAAAALAEPGDAVLLSPACASLDMFRN
HHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHH
YAHRADVFRAAVDELAIDKGATP
HHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA