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The map label for this gene is ampD [H]

Identifier: 161525959

GI number: 161525959

Start: 3044767

End: 3045357

Strand: Reverse

Name: ampD [H]

Synonym: Bmul_2790

Alternate gene names: 161525959

Gene position: 3045357-3044767 (Counterclockwise)

Preceding gene: 161525960

Following gene: 161525958

Centisome position: 88.31

GC content: 70.56

Gene sequence:

>591_bases
ATGACCGACGCGCGGCGGCTGTCGGTCGACGCGGCCGGCTGGGTGCGCGAAGCGCGCCACGCGCGCTCGCCGAACTTCGA
GGCGCGGCCCGCGGGTGCGGTGCCGTCGCTCGTGATCGTGCACAACATCAGCCTGCCGCCCGGCGAGTTCGGCGGCGACG
CGATCGAGGCGCTGTTCCTGAATCGCCTCGACTGCGACGCGCACCCGTACTACCAGCGTCACCTGCGCGACGTGCGCGTC
TCCGCCCATTTCCTGATCCGCCGCGACGGCGAGCTCGTGCAGTTCGTGTCGTGCGACGAGCGCGCGTGGCACGCCGGTGC
GTCCGAGTTTTTCGGCCGGCCGCGCTGCAACGACTTCTCGATCGGCATCGAGCTCGAAGGCGCCGACGACGTGCCGTTCG
ACGATGCGCAATATGCGACGCTCGCCGTGCTCGCGCGCACGCTCGCCGCGCGCTATCCGGTCGACGCCTTCGCCGGGCAT
TCCGACGTCGCGCCCGGCCGCAAGACGGACCCCGGTCCGCATTTCGATTGGCAACGCTTCGCGCGCGATGCCGGGTTCGG
CGCCGAATACTTTCCTTTCCGGCAGCAGTGA

Upstream 100 bases:

>100_bases
TGCATGCGCCGAAGGGCGATGCGATCGCGGCCGCGAGCGGCGAATACTTCTGCAGCACCGAGCACGCGCAACGTCACGCC
GCACGCGCGGGCGGCGCGCG

Downstream 100 bases:

>100_bases
GCGCAGGGTTTTTTGACCGGAAAAACCCTTTTCTCGGATCAATGCCTTGTCGCGTCTGCCCGTGCGCGATGTCAAGACCT
CCGGCGCAAATTCTCCGTTT

Product: N-acetyl-anhydromuranmyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 196; Mature: 195

Protein sequence:

>196_residues
MTDARRLSVDAAGWVREARHARSPNFEARPAGAVPSLVIVHNISLPPGEFGGDAIEALFLNRLDCDAHPYYQRHLRDVRV
SAHFLIRRDGELVQFVSCDERAWHAGASEFFGRPRCNDFSIGIELEGADDVPFDDAQYATLAVLARTLAARYPVDAFAGH
SDVAPGRKTDPGPHFDWQRFARDAGFGAEYFPFRQQ

Sequences:

>Translated_196_residues
MTDARRLSVDAAGWVREARHARSPNFEARPAGAVPSLVIVHNISLPPGEFGGDAIEALFLNRLDCDAHPYYQRHLRDVRV
SAHFLIRRDGELVQFVSCDERAWHAGASEFFGRPRCNDFSIGIELEGADDVPFDDAQYATLAVLARTLAARYPVDAFAGH
SDVAPGRKTDPGPHFDWQRFARDAGFGAEYFPFRQQ
>Mature_195_residues
TDARRLSVDAAGWVREARHARSPNFEARPAGAVPSLVIVHNISLPPGEFGGDAIEALFLNRLDCDAHPYYQRHLRDVRVS
AHFLIRRDGELVQFVSCDERAWHAGASEFFGRPRCNDFSIGIELEGADDVPFDDAQYATLAVLARTLAARYPVDAFAGHS
DVAPGRKTDPGPHFDWQRFARDAGFGAEYFPFRQQ

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1786300, Length=170, Percent_Identity=57.0588235294118, Blast_Score=197, Evalue=3e-52,
Organism=Escherichia coli, GI1787092, Length=132, Percent_Identity=41.6666666666667, Blast_Score=85, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 21869; Mature: 21738

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDARRLSVDAAGWVREARHARSPNFEARPAGAVPSLVIVHNISLPPGEFGGDAIEALFL
CCCCCEEECCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHH
NRLDCDAHPYYQRHLRDVRVSAHFLIRRDGELVQFVSCDERAWHAGASEFFGRPRCNDFS
HHCCCCCCHHHHHHHHHHHHHEEEEEECCCCEEEEECCCCHHHHCCHHHHCCCCCCCCEE
IGIELEGADDVPFDDAQYATLAVLARTLAARYPVDAFAGHSDVAPGRKTDPGPHFDWQRF
EEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCHHHH
ARDAGFGAEYFPFRQQ
HHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TDARRLSVDAAGWVREARHARSPNFEARPAGAVPSLVIVHNISLPPGEFGGDAIEALFL
CCCCEEECCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHH
NRLDCDAHPYYQRHLRDVRVSAHFLIRRDGELVQFVSCDERAWHAGASEFFGRPRCNDFS
HHCCCCCCHHHHHHHHHHHHHEEEEEECCCCEEEEECCCCHHHHCCHHHHCCCCCCCCEE
IGIELEGADDVPFDDAQYATLAVLARTLAARYPVDAFAGHSDVAPGRKTDPGPHFDWQRF
EEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCHHHH
ARDAGFGAEYFPFRQQ
HHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 8419294 [H]