Click here to switch to the map view.

The map label for this gene is purB [H]

Identifier: 161525886

GI number: 161525886

Start: 2968484

End: 2969932

Strand: Direct

Name: purB [H]

Synonym: Bmul_2717

Alternate gene names: 161525886

Gene position: 2968484-2969932 (Clockwise)

Preceding gene: 161525879

Following gene: 161525888

Centisome position: 86.08

GC content: 65.29

Gene sequence:

>1449_bases
ATGGTAAAATCCCGGATTCTCCTCTCCACGCTCGACCCGGCCCCCAAGGCCCGCCCGATCATGTCCGACACTCGTCCCGA
TACGCTTTTCGCGCTCACCGCGCTCTCGCCCATCGACGGCCGCTACGCCAGCAAGACCGAAGCGCTGCGCGACTGGCTCT
CCGAAGCCGCCTTCATGCGTCACCGCGTCACCGTCGAGGTGCACTGGCTGATCGCGCTGTCGCGCGCCGGTTTCGCCGAA
GTCCCGCGCTTCTCTGAAGCCGCCGAGCAGTTCCTGCTGCAGCTCGTCGAGCGCTTCACCGCGCACGATGCCGCGCGCAT
CAAGGAAATCGAGCGCGTGACGAACCACGACGTGAAGGCCGTCGAATACTGGCTGAAGGAATCGGTGAAGGGCCAGCCCG
AACTCGAGAAGGCCAGCGAATTCATCCACTTCGCCTGCACGTCCGAGGACATCAACAACACGTCGCACGGGATGATGCTC
GCCGGCGCGCGCGAACACGTGATCCTGCCGGCGCTGCGCACGGTCCACCAGCGTCTCGTCGCGCTCGCGCATGCGCACGC
CGAGCAGCCGATGCTGTCGCGCACGCACGGCCAGCCGGCCAGCCCGACGACGCTCGGCAAGGAACTCGCGAACGTCGCCG
CGCGGCTCGCACGTGCGATCGCGCGCATCGAGAAGGTCGAAATCCTCGGCAAGATGAACGGTGCGGTCGGCAACTTCAAC
GCGCACCTGTCCGCGTATCCGGAATTCGACTGGGAAGCGTTCTCGCGCGACGTGATCGAAAACCGCCTGAAGCTCACGTT
CAACCCGTACACGATCCAGATCGAGCCGCACGACTACATGGCCGAACTGTTCGACGCGGTCGCGCGCGCGAACACGATCC
TGCTCGACCTCGACCGCGACGTCTGGGGCTACATCGCGCTCGGCTACTTCAAGCAGAAGACGAAGGCCGGCGAAATCGGC
TCGTCGACGATGCCGCACAAGGTCAACCCGATCGACTTCGAGAACTCGGAAGGCAACCTCGGCCTCGCGAACGCGACGCT
GCGCCACCTCGCCGACAAGCTGCCGGTATCGCGCTGGCAGCGTGACCTGACCGACTCGACGGTCCTGCGCAACATGGGCG
TCGCGCTCGGCTATTCGCTGCTCGCGTACGACGCGCTGATCCGCGGCCTCGACAAGCTCGAAGTGAACCCGCAGCGCCTC
AACGAAGATCTCGACAACTGCTGGGAAGTGCTGGCCGAACCGGTGCAGACGGTGATGCGCCGCTACGGAATCGAGAACCC
GTACGAGCAACTGAAGGAGCTGACGCGCGGCAAGGGCATCACGCGCGAAGCGCTGCAGCAGTTCATCGGCACGCTCGCGA
TTCCGCAGGACGCGAAGGACCGCCTGCTCGCGATGACGCCCGCGTCGTATATCGGCAAGGCCGTCGAACTCGCGAAGCGG
ATCGCGTAA

Upstream 100 bases:

>100_bases
GGGCGGGCGCGGCAGGCGGCGAGCGGAAGAAGGCTGCATTTTACCGGCGCCGCGCGGGGCCATCGCAGGCGGCCGCGCGC
GACGGAGCCGGCGCGCGCCG

Downstream 100 bases:

>100_bases
CGGCACGGCGGCGCACGTTCGCGCCGTTTCGTCGCCCGCACGCTCCATAGCGAAACGCCCGATGCGATGCATCGGGCGTT
TTCGTTTTCATGCGCGGTTG

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MVKSRILLSTLDPAPKARPIMSDTRPDTLFALTALSPIDGRYASKTEALRDWLSEAAFMRHRVTVEVHWLIALSRAGFAE
VPRFSEAAEQFLLQLVERFTAHDAARIKEIERVTNHDVKAVEYWLKESVKGQPELEKASEFIHFACTSEDINNTSHGMML
AGAREHVILPALRTVHQRLVALAHAHAEQPMLSRTHGQPASPTTLGKELANVAARLARAIARIEKVEILGKMNGAVGNFN
AHLSAYPEFDWEAFSRDVIENRLKLTFNPYTIQIEPHDYMAELFDAVARANTILLDLDRDVWGYIALGYFKQKTKAGEIG
SSTMPHKVNPIDFENSEGNLGLANATLRHLADKLPVSRWQRDLTDSTVLRNMGVALGYSLLAYDALIRGLDKLEVNPQRL
NEDLDNCWEVLAEPVQTVMRRYGIENPYEQLKELTRGKGITREALQQFIGTLAIPQDAKDRLLAMTPASYIGKAVELAKR
IA

Sequences:

>Translated_482_residues
MVKSRILLSTLDPAPKARPIMSDTRPDTLFALTALSPIDGRYASKTEALRDWLSEAAFMRHRVTVEVHWLIALSRAGFAE
VPRFSEAAEQFLLQLVERFTAHDAARIKEIERVTNHDVKAVEYWLKESVKGQPELEKASEFIHFACTSEDINNTSHGMML
AGAREHVILPALRTVHQRLVALAHAHAEQPMLSRTHGQPASPTTLGKELANVAARLARAIARIEKVEILGKMNGAVGNFN
AHLSAYPEFDWEAFSRDVIENRLKLTFNPYTIQIEPHDYMAELFDAVARANTILLDLDRDVWGYIALGYFKQKTKAGEIG
SSTMPHKVNPIDFENSEGNLGLANATLRHLADKLPVSRWQRDLTDSTVLRNMGVALGYSLLAYDALIRGLDKLEVNPQRL
NEDLDNCWEVLAEPVQTVMRRYGIENPYEQLKELTRGKGITREALQQFIGTLAIPQDAKDRLLAMTPASYIGKAVELAKR
IA
>Mature_482_residues
MVKSRILLSTLDPAPKARPIMSDTRPDTLFALTALSPIDGRYASKTEALRDWLSEAAFMRHRVTVEVHWLIALSRAGFAE
VPRFSEAAEQFLLQLVERFTAHDAARIKEIERVTNHDVKAVEYWLKESVKGQPELEKASEFIHFACTSEDINNTSHGMML
AGAREHVILPALRTVHQRLVALAHAHAEQPMLSRTHGQPASPTTLGKELANVAARLARAIARIEKVEILGKMNGAVGNFN
AHLSAYPEFDWEAFSRDVIENRLKLTFNPYTIQIEPHDYMAELFDAVARANTILLDLDRDVWGYIALGYFKQKTKAGEIG
SSTMPHKVNPIDFENSEGNLGLANATLRHLADKLPVSRWQRDLTDSTVLRNMGVALGYSLLAYDALIRGLDKLEVNPQRL
NEDLDNCWEVLAEPVQTVMRRYGIENPYEQLKELTRGKGITREALQQFIGTLAIPQDAKDRLLAMTPASYIGKAVELAKR
IA

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787376, Length=454, Percent_Identity=62.9955947136564, Blast_Score=590, Evalue=1e-170,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 54048; Mature: 54048

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKSRILLSTLDPAPKARPIMSDTRPDTLFALTALSPIDGRYASKTEALRDWLSEAAFMR
CCCCCEEHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
HRVTVEVHWLIALSRAGFAEVPRFSEAAEQFLLQLVERFTAHDAARIKEIERVTNHDVKA
HHEEEEEEEEEHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
VEYWLKESVKGQPELEKASEFIHFACTSEDINNTSHGMMLAGAREHVILPALRTVHQRLV
HHHHHHHHCCCCCCHHHHHHHEEEEECCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHH
ALAHAHAEQPMLSRTHGQPASPTTLGKELANVAARLARAIARIEKVEILGKMNGAVGNFN
HHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AHLSAYPEFDWEAFSRDVIENRLKLTFNPYTIQIEPHDYMAELFDAVARANTILLDLDRD
CCEECCCCCCHHHHHHHHHHCCCEEEECCEEEEECCHHHHHHHHHHHHHCCEEEEECCCC
VWGYIALGYFKQKTKAGEIGSSTMPHKVNPIDFENSEGNLGLANATLRHLADKLPVSRWQ
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHH
RDLTDSTVLRNMGVALGYSLLAYDALIRGLDKLEVNPQRLNEDLDNCWEVLAEPVQTVMR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RYGIENPYEQLKELTRGKGITREALQQFIGTLAIPQDAKDRLLAMTPASYIGKAVELAKR
HCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHH
IA
CC
>Mature Secondary Structure
MVKSRILLSTLDPAPKARPIMSDTRPDTLFALTALSPIDGRYASKTEALRDWLSEAAFMR
CCCCCEEHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
HRVTVEVHWLIALSRAGFAEVPRFSEAAEQFLLQLVERFTAHDAARIKEIERVTNHDVKA
HHEEEEEEEEEHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
VEYWLKESVKGQPELEKASEFIHFACTSEDINNTSHGMMLAGAREHVILPALRTVHQRLV
HHHHHHHHCCCCCCHHHHHHHEEEEECCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHH
ALAHAHAEQPMLSRTHGQPASPTTLGKELANVAARLARAIARIEKVEILGKMNGAVGNFN
HHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AHLSAYPEFDWEAFSRDVIENRLKLTFNPYTIQIEPHDYMAELFDAVARANTILLDLDRD
CCEECCCCCCHHHHHHHHHHCCCEEEECCEEEEECCHHHHHHHHHHHHHCCEEEEECCCC
VWGYIALGYFKQKTKAGEIGSSTMPHKVNPIDFENSEGNLGLANATLRHLADKLPVSRWQ
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHH
RDLTDSTVLRNMGVALGYSLLAYDALIRGLDKLEVNPQRLNEDLDNCWEVLAEPVQTVMR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RYGIENPYEQLKELTRGKGITREALQQFIGTLAIPQDAKDRLLAMTPASYIGKAVELAKR
HCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHH
IA
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]