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The map label for this gene is yafJ [C]

Identifier: 161525878

GI number: 161525878

Start: 2955578

End: 2956564

Strand: Direct

Name: yafJ [C]

Synonym: Bmul_2709

Alternate gene names: 161525878

Gene position: 2955578-2956564 (Clockwise)

Preceding gene: 161525875

Following gene: 161525879

Centisome position: 85.71

GC content: 65.45

Gene sequence:

>987_bases
ATGCTAGAAGCCGCATTGTACCGGGGGTTCGCCCGGTTCGTTACCCCGCTTCAGTCTGCCGCCCCAAGTAAAATGTGCCA
ACTCCTAGGAATGAACTGCGCCGCGCCGACGGACGTCACATTCTCCTTCACAGGGTTCGCGGCCCGGGGCGGCCTCACCG
ATCACCACGCCGACGGCTGGGGCATCGCATTCTTCGAGGACAAGGCGTGCCGCCTGTTCATCGACCAGCAGGCATCGGCC
ACCTCGCCGATCGCCGAAATGGTCAAGCGCTATCCGATCAAGTCGAAGAACACGATCGCGCATATCCGCAAGGCCACGCA
AGGCCACATCGTGCTGGAGAACAGTCACCCGTTCATGCGCGAGCTGTGGGGGCGACACTGGATCTTCGCGCATAACGGCG
ATCTGCAGGACTTCGAACCCGAGCTCGAGCGCAGCGTGTACCAGCCGGTCGGCACGACCGACAGCGAGAAGGCGTTCTGC
GTGCTGATGCAGGGGCTGCGCGAGGCGTTTCCCGGCGCGCAGCCGCCGCTGCCGGAACTGTTCGAGCGCGTCGGCGAGCT
CACGCGCGGGATCACCGATCACGGCGTGTTCAATTTCCTGATGTCGAACGGCCAGGCGCTGTTTGCGCACTGCTCGACGC
GGCTGCACTACCTCGTGCGCCGCTGGCCATTCTCGACCGCGCATCTCGTCGACGAAGACCTGTCGATCGACTTCGCGAAA
TACACGACGCCCGAGGATCGCGTGGCGGTGATCGCGACGCAGCCGCTCACCGACAATGAAGTGTGGACGGCCTTCGAGCC
CGGCGAGCTGATCATGTTCCAGTGCGGCGACGTCGCCGCGCGGATGCAGATTCCGGTGCCGGAGCGCGTGCTCGAGAAGC
TGCGCAATCCGTCGCTCGATCCGTCGGCGTCCGCGCCGTGCACGACGCGCACGGCGGCGCTCGCGACCGCCGCCGACGGC
GACCCGGACGACACGATCGATTTCTGA

Upstream 100 bases:

>100_bases
GGGGCCGGACCGCGCGTGCGCGGCCCGCGACTGACGGCAAACCGCAACACGGGGCCTTCGGCCGGCGGAATCCGCTTTTT
ACCGCTCGAGGCTCGGTGAT

Downstream 100 bases:

>100_bases
GCGCGCCCTGCGCGCCCGCGCCGCCATCGGGCAGCCGCGGCCATTCCCGTCGACACAACGCGCATTCACGCGGTGCCGTT
TCATCCGCGCGACAGGAAGA

Product: glutamine amidotransferase class-II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MLEAALYRGFARFVTPLQSAAPSKMCQLLGMNCAAPTDVTFSFTGFAARGGLTDHHADGWGIAFFEDKACRLFIDQQASA
TSPIAEMVKRYPIKSKNTIAHIRKATQGHIVLENSHPFMRELWGRHWIFAHNGDLQDFEPELERSVYQPVGTTDSEKAFC
VLMQGLREAFPGAQPPLPELFERVGELTRGITDHGVFNFLMSNGQALFAHCSTRLHYLVRRWPFSTAHLVDEDLSIDFAK
YTTPEDRVAVIATQPLTDNEVWTAFEPGELIMFQCGDVAARMQIPVPERVLEKLRNPSLDPSASAPCTTRTAALATAADG
DPDDTIDF

Sequences:

>Translated_328_residues
MLEAALYRGFARFVTPLQSAAPSKMCQLLGMNCAAPTDVTFSFTGFAARGGLTDHHADGWGIAFFEDKACRLFIDQQASA
TSPIAEMVKRYPIKSKNTIAHIRKATQGHIVLENSHPFMRELWGRHWIFAHNGDLQDFEPELERSVYQPVGTTDSEKAFC
VLMQGLREAFPGAQPPLPELFERVGELTRGITDHGVFNFLMSNGQALFAHCSTRLHYLVRRWPFSTAHLVDEDLSIDFAK
YTTPEDRVAVIATQPLTDNEVWTAFEPGELIMFQCGDVAARMQIPVPERVLEKLRNPSLDPSASAPCTTRTAALATAADG
DPDDTIDF
>Mature_328_residues
MLEAALYRGFARFVTPLQSAAPSKMCQLLGMNCAAPTDVTFSFTGFAARGGLTDHHADGWGIAFFEDKACRLFIDQQASA
TSPIAEMVKRYPIKSKNTIAHIRKATQGHIVLENSHPFMRELWGRHWIFAHNGDLQDFEPELERSVYQPVGTTDSEKAFC
VLMQGLREAFPGAQPPLPELFERVGELTRGITDHGVFNFLMSNGQALFAHCSTRLHYLVRRWPFSTAHLVDEDLSIDFAK
YTTPEDRVAVIATQPLTDNEVWTAFEPGELIMFQCGDVAARMQIPVPERVLEKLRNPSLDPSASAPCTTRTAALATAADG
DPDDTIDF

Specific function: Unknown

COG id: COG0121

COG function: function code R; Predicted glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786417, Length=253, Percent_Identity=51.3833992094862, Blast_Score=280, Evalue=9e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932 [H]

Pfam domain/function: PF00310 GATase_2 [H]

EC number: NA

Molecular weight: Translated: 36435; Mature: 36435

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEAALYRGFARFVTPLQSAAPSKMCQLLGMNCAAPTDVTFSFTGFAARGGLTDHHADGW
CCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCEEEECCCHHCCCCCCCCCCCC
GIAFFEDKACRLFIDQQASATSPIAEMVKRYPIKSKNTIAHIRKATQGHIVLENSHPFMR
EEEEEECCCEEEEEECCCCCCCHHHHHHHHCCCCCCCHHHHHHHCCCCCEEEECCCHHHH
ELWGRHWIFAHNGDLQDFEPELERSVYQPVGTTDSEKAFCVLMQGLREAFPGAQPPLPEL
HHCCCEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHH
FERVGELTRGITDHGVFNFLMSNGQALFAHCSTRLHYLVRRWPFSTAHLVDEDLSIDFAK
HHHHHHHHCCCHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHCCCCHHHHHCCCCCEEEHH
YTTPEDRVAVIATQPLTDNEVWTAFEPGELIMFQCGDVAARMQIPVPERVLEKLRNPSLD
CCCCCCCEEEEEECCCCCCCCEEEECCCCEEEEECCCEEEEEECCCHHHHHHHHCCCCCC
PSASAPCTTRTAALATAADGDPDDTIDF
CCCCCCCCCHHHHEEECCCCCCCCCCCC
>Mature Secondary Structure
MLEAALYRGFARFVTPLQSAAPSKMCQLLGMNCAAPTDVTFSFTGFAARGGLTDHHADGW
CCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCEEEECCCHHCCCCCCCCCCCC
GIAFFEDKACRLFIDQQASATSPIAEMVKRYPIKSKNTIAHIRKATQGHIVLENSHPFMR
EEEEEECCCEEEEEECCCCCCCHHHHHHHHCCCCCCCHHHHHHHCCCCCEEEECCCHHHH
ELWGRHWIFAHNGDLQDFEPELERSVYQPVGTTDSEKAFCVLMQGLREAFPGAQPPLPEL
HHCCCEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHH
FERVGELTRGITDHGVFNFLMSNGQALFAHCSTRLHYLVRRWPFSTAHLVDEDLSIDFAK
HHHHHHHHCCCHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHCCCCHHHHHCCCCCEEEHH
YTTPEDRVAVIATQPLTDNEVWTAFEPGELIMFQCGDVAARMQIPVPERVLEKLRNPSLD
CCCCCCCEEEEEECCCCCCCCEEEECCCCEEEEECCCEEEEEECCCHHHHHHHHCCCCCC
PSASAPCTTRTAALATAADGDPDDTIDF
CCCCCCCCCHHHHEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]