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The map label for this gene is purH [H]

Identifier: 161525862

GI number: 161525862

Start: 2940776

End: 2942341

Strand: Direct

Name: purH [H]

Synonym: Bmul_2693

Alternate gene names: 161525862

Gene position: 2940776-2942341 (Clockwise)

Preceding gene: 161525861

Following gene: 161525863

Centisome position: 85.28

GC content: 65.64

Gene sequence:

>1566_bases
ATGATCAAGCAAGCGCTCATTTCCGTTTCCGACAAGACCGGCATCGTCGACTTCGCGAAGTCGCTGTCCGACCTCGGCGT
CAAGCTGCTGTCGACGGGCGGCACCGCGAAACTGCTCGCCGACGCGGGCCTGCCCGTCACCGAAGTGGCGGACTACACGG
GCTTTCCGGAAATGCTCGATGGGCGCGTGAAGACGCTGCATCCGAAGGTGCACGGCGGCATCCTCGCGCGTCGCGATCTG
CCCGAGCACATGCAGGCACTGGAAGCGCACGACATCCCGACGATCGACCTGCTCGTCGTGAACCTTTATCCGTTCGTCGC
GACGATCGCGAAGGACGATTGCACGCTCGCCGACGCGATCGAGAACATCGACATCGGCGGCCCGACGATGCTGCGCTCGG
CCGCGAAGAACCACCGCGACGTGACGGTGATCGTCGATCCGGCCGACTACGCGGTCGTGCTCGACGAAATGAAGGCGAAC
GGCAACACGGTCGGCTACGCGACGAACTTCCGGCTCGCGACGAAGGTGTTCGCGCACACCGCGCAATACGACGGCGCGAT
CACGAACTACCTGACGAGCCTCACCGACGAACTGAAGCACGGCTCGCGCAATCCGTACCCGGCGACGCTGAACATGGCGT
TCGAGAAGGTGCAGGACCTGCGCTACGGCGAGAACCCGCACCAGAGCGCGGCGTTCTATCGCGACATCGTCACGCCGGCC
GGCGCGCTCGCGAACTACCGCCAGCTGCAGGGCAAGGAACTGTCGTACAACAACATCGCCGATTCGGACGCGGCGTGGGA
ATGCGTGAAGACGTTCGACGCGCCGGCCTGCGTGATCATCAAGCATGCGAACCCGTGCGGCGTCGCGGTCGGCAACGACT
CGGCCGATGCGTACGCGAAGGCGTTCCAGACCGACCCGACGTCGGCGTTCGGCGGCATCATCGCGTTCAACCGCGAAGTC
GACGAAGCCGCGGCGCAGGCCGTCGCCAAGCAGTTCGTCGAAGTGCTGATCGCGCCGTCGTTCACCGATGCCGCGAAGCA
GGTGTTCGCCGCGAAGCAGAACGTGCGTCTGCTCGAGATTGCGCTGGGCGAAGGCCATAACGCGTTCGACCTGAAGCGTG
TCGGCGGCGGCCTGCTCGTGCAGTCGCTCGATGCAAAGAACGTGCAGCCGCACGAGCTGCGCGTCGTCACGAAGCGTCAT
CCGACGCCGAAGGAGATGGACGACCTGCTGTTCGCGTGGCGCGTCGCGAAGTACGTGAAGTCGAACGCGATCGTGTTCTG
CGGCAACGGCATGACGCTCGGCGTCGGTGCCGGCCAGATGAGCCGCGTCGACTCCGCGCGCATCGCAAGCATCAAGGCGC
AGAACGCGGGCCTGACGCTGGCCGGTTCGGCCGTCGCGTCGGATGCGTTCTTCCCGTTCCGCGACGGTCTGGACGTGGTC
GTCGCGGCAGGCGCGACCTGCGTGATCCAGCCGGGCGGCTCGGTGCGCGACGACGAAGTGATCGCGGCAGCCGACGAGCA
CAATATCGCGATGATCCTGACGGGCGTGCGTCACTTCCGTCACTGA

Upstream 100 bases:

>100_bases
TCGGCATCAACCGCAATACGCTGCGCAAGAAGCTGCAGCAGCACGGCCTGCTGTAGTCGGCCGCCCCGTCCCCGTTTCCC
TGGCTATTGGTGGTTCCATC

Downstream 100 bases:

>100_bases
TCGCTCGCATGCGGCCCGCGCGGCCGCTGCACCCATACCCGGCGGCGTCGCACCCGCCGGGTTTTTTATTGCGCGGCGCA
TGAGCGGCCGGCCGTGCGCG

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 521; Mature: 521

Protein sequence:

>521_residues
MIKQALISVSDKTGIVDFAKSLSDLGVKLLSTGGTAKLLADAGLPVTEVADYTGFPEMLDGRVKTLHPKVHGGILARRDL
PEHMQALEAHDIPTIDLLVVNLYPFVATIAKDDCTLADAIENIDIGGPTMLRSAAKNHRDVTVIVDPADYAVVLDEMKAN
GNTVGYATNFRLATKVFAHTAQYDGAITNYLTSLTDELKHGSRNPYPATLNMAFEKVQDLRYGENPHQSAAFYRDIVTPA
GALANYRQLQGKELSYNNIADSDAAWECVKTFDAPACVIIKHANPCGVAVGNDSADAYAKAFQTDPTSAFGGIIAFNREV
DEAAAQAVAKQFVEVLIAPSFTDAAKQVFAAKQNVRLLEIALGEGHNAFDLKRVGGGLLVQSLDAKNVQPHELRVVTKRH
PTPKEMDDLLFAWRVAKYVKSNAIVFCGNGMTLGVGAGQMSRVDSARIASIKAQNAGLTLAGSAVASDAFFPFRDGLDVV
VAAGATCVIQPGGSVRDDEVIAAADEHNIAMILTGVRHFRH

Sequences:

>Translated_521_residues
MIKQALISVSDKTGIVDFAKSLSDLGVKLLSTGGTAKLLADAGLPVTEVADYTGFPEMLDGRVKTLHPKVHGGILARRDL
PEHMQALEAHDIPTIDLLVVNLYPFVATIAKDDCTLADAIENIDIGGPTMLRSAAKNHRDVTVIVDPADYAVVLDEMKAN
GNTVGYATNFRLATKVFAHTAQYDGAITNYLTSLTDELKHGSRNPYPATLNMAFEKVQDLRYGENPHQSAAFYRDIVTPA
GALANYRQLQGKELSYNNIADSDAAWECVKTFDAPACVIIKHANPCGVAVGNDSADAYAKAFQTDPTSAFGGIIAFNREV
DEAAAQAVAKQFVEVLIAPSFTDAAKQVFAAKQNVRLLEIALGEGHNAFDLKRVGGGLLVQSLDAKNVQPHELRVVTKRH
PTPKEMDDLLFAWRVAKYVKSNAIVFCGNGMTLGVGAGQMSRVDSARIASIKAQNAGLTLAGSAVASDAFFPFRDGLDVV
VAAGATCVIQPGGSVRDDEVIAAADEHNIAMILTGVRHFRH
>Mature_521_residues
MIKQALISVSDKTGIVDFAKSLSDLGVKLLSTGGTAKLLADAGLPVTEVADYTGFPEMLDGRVKTLHPKVHGGILARRDL
PEHMQALEAHDIPTIDLLVVNLYPFVATIAKDDCTLADAIENIDIGGPTMLRSAAKNHRDVTVIVDPADYAVVLDEMKAN
GNTVGYATNFRLATKVFAHTAQYDGAITNYLTSLTDELKHGSRNPYPATLNMAFEKVQDLRYGENPHQSAAFYRDIVTPA
GALANYRQLQGKELSYNNIADSDAAWECVKTFDAPACVIIKHANPCGVAVGNDSADAYAKAFQTDPTSAFGGIIAFNREV
DEAAAQAVAKQFVEVLIAPSFTDAAKQVFAAKQNVRLLEIALGEGHNAFDLKRVGGGLLVQSLDAKNVQPHELRVVTKRH
PTPKEMDDLLFAWRVAKYVKSNAIVFCGNGMTLGVGAGQMSRVDSARIASIKAQNAGLTLAGSAVASDAFFPFRDGLDVV
VAAGATCVIQPGGSVRDDEVIAAADEHNIAMILTGVRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=479, Percent_Identity=37.5782881002088, Blast_Score=272, Evalue=5e-73,
Organism=Escherichia coli, GI1790439, Length=525, Percent_Identity=61.5238095238095, Blast_Score=652, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=609, Percent_Identity=33.6617405582923, Blast_Score=288, Evalue=3e-78,
Organism=Caenorhabditis elegans, GI71985574, Length=317, Percent_Identity=27.4447949526814, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71985556, Length=130, Percent_Identity=34.6153846153846, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323056, Length=483, Percent_Identity=37.0600414078675, Blast_Score=266, Evalue=5e-72,
Organism=Saccharomyces cerevisiae, GI6323768, Length=480, Percent_Identity=36.6666666666667, Blast_Score=266, Evalue=5e-72,
Organism=Drosophila melanogaster, GI24649832, Length=485, Percent_Identity=37.5257731958763, Blast_Score=269, Evalue=3e-72,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 55714; Mature: 55714

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKQALISVSDKTGIVDFAKSLSDLGVKLLSTGGTAKLLADAGLPVTEVADYTGFPEMLD
CCCHHHEECCCCCCHHHHHHHHHHCCEEEECCCCCEEEHCCCCCCHHHHHHCCCCHHHHC
GRVKTLHPKVHGGILARRDLPEHMQALEAHDIPTIDLLVVNLYPFVATIAKDDCTLADAI
CCCEEECCHHHCCEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHH
ENIDIGGPTMLRSAAKNHRDVTVIVDPADYAVVLDEMKANGNTVGYATNFRLATKVFAHT
HHCCCCCHHHHHHHHCCCCCEEEEECCCCEEEEEEHHCCCCCEEEEEECHHHHHHHHHHH
AQYDGAITNYLTSLTDELKHGSRNPYPATLNMAFEKVQDLRYGENPHQSAAFYRDIVTPA
HHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCH
GALANYRQLQGKELSYNNIADSDAAWECVKTFDAPACVIIKHANPCGVAVGNDSADAYAK
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCEEEECCCCHHHHHH
AFQTDPTSAFGGIIAFNREVDEAAAQAVAKQFVEVLIAPSFTDAAKQVFAAKQNVRLLEI
HHCCCCCHHHCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEE
ALGEGHNAFDLKRVGGGLLVQSLDAKNVQPHELRVVTKRHPTPKEMDDLLFAWRVAKYVK
EECCCCCCEEHHHCCCCEEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
SNAIVFCGNGMTLGVGAGQMSRVDSARIASIKAQNAGLTLAGSAVASDAFFPFRDGLDVV
CCEEEEECCCEEEEECCCCHHHCCCHHHEEEEECCCCEEEECCHHHCCCCCCCCCCCEEE
VAAGATCVIQPGGSVRDDEVIAAADEHNIAMILTGVRHFRH
EECCCEEEECCCCCCCCCCEEEECCCCCEEEEEECHHHHCC
>Mature Secondary Structure
MIKQALISVSDKTGIVDFAKSLSDLGVKLLSTGGTAKLLADAGLPVTEVADYTGFPEMLD
CCCHHHEECCCCCCHHHHHHHHHHCCEEEECCCCCEEEHCCCCCCHHHHHHCCCCHHHHC
GRVKTLHPKVHGGILARRDLPEHMQALEAHDIPTIDLLVVNLYPFVATIAKDDCTLADAI
CCCEEECCHHHCCEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHH
ENIDIGGPTMLRSAAKNHRDVTVIVDPADYAVVLDEMKANGNTVGYATNFRLATKVFAHT
HHCCCCCHHHHHHHHCCCCCEEEEECCCCEEEEEEHHCCCCCEEEEEECHHHHHHHHHHH
AQYDGAITNYLTSLTDELKHGSRNPYPATLNMAFEKVQDLRYGENPHQSAAFYRDIVTPA
HHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCH
GALANYRQLQGKELSYNNIADSDAAWECVKTFDAPACVIIKHANPCGVAVGNDSADAYAK
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCEEEECCCCHHHHHH
AFQTDPTSAFGGIIAFNREVDEAAAQAVAKQFVEVLIAPSFTDAAKQVFAAKQNVRLLEI
HHCCCCCHHHCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEE
ALGEGHNAFDLKRVGGGLLVQSLDAKNVQPHELRVVTKRHPTPKEMDDLLFAWRVAKYVK
EECCCCCCEEHHHCCCCEEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
SNAIVFCGNGMTLGVGAGQMSRVDSARIASIKAQNAGLTLAGSAVASDAFFPFRDGLDVV
CCEEEEECCCEEEEECCCCHHHCCCHHHEEEEECCCCEEEECCHHHCCCCCCCCCCCEEE
VAAGATCVIQPGGSVRDDEVIAAADEHNIAMILTGVRHFRH
EECCCEEEECCCCCCCCCCEEEECCCCCEEEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA