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The map label for this gene is rlmL [H]

Identifier: 161525846

GI number: 161525846

Start: 2924357

End: 2925655

Strand: Direct

Name: rlmL [H]

Synonym: Bmul_2677

Alternate gene names: 161525846

Gene position: 2924357-2925655 (Clockwise)

Preceding gene: 161525845

Following gene: 161525847

Centisome position: 84.8

GC content: 71.9

Gene sequence:

>1299_bases
ATGTCCTCGCCCACCCTGTACGACTTTTTCGCTCCCTGCCCGCGCGGCCTCGAAGCGGCGCTCGCCGCCGAGCTGGCCGA
GATCGCCGGCCGTCACCTGGACGGCGCGCCGTTCGCCGCGGGCGCGCAGGTGCCGGGCGGCGTCCATTTCCGCGGCGGCT
GGGCCGCCGGGATGGCCGCGAACCTGCACTCGCGGATCGCGAGCCGGATTCTGCTGAAGATCGCGCATCGCCCGTACCGC
AGCGAGCAGGATGTCTACGCGCTCGCGCTCGAGCAGCCGTGGGAGCGCTGGTTCGCGGCGACGCAGACGCTGCGCGTCGA
CATCACCGCGATCAAGTCGCCGCTGAAGAGCCTCGAATTCGCGACGCTGCGCGTCAAGGACGCGATCTGCGACCGAATGC
GCGACAAGACCGGCGCGCGCCCGAGCATCGACACCGGCGCGCCCGACGTGCGCGTGTTCGCGTTCCTGACGGCCACCGAC
TGCACGCTGTACCTCGACACGTCCGGCGAGCCGCTGTTCAAGCGCGGCTGGCGTCTCGACAAGGGCGCGGCGCCGCTGCG
CGAGAACCTCGCGGCCGGCATCCTGCGCCTGACCGGCTGGACGCCCGGCACGCCGCTCTACGACCCGATGTGCGGCAGCG
GCACGTTCCTCGCGGAAGCCGCGCAAATGGTGCTCGGCGTCGCGCCGGGCGTCGAGCGCCGATTCGGTTTCGAGAAGCTG
AAGCAGTACGACATCACGGCCTGGCAGGGGCTGAAGGTCGCCGCGCTCGACGCGAAGCGCGCGGCACGCGGCCGGCGCGA
CGATCTGCGCGTCTACGGCAGCGACATCTCCGGCGACATGCTCGAGAAGGCGCGCGCAAACCTCGAGCGCGCCGGCGTGC
CGTCCGTGTGGCTCAAGCAGGTCGATGCGCGCGGCATGACGCCGCCGTGCGACGGGCCGGGCCTGATCGTCGCGAATCCG
CCGTACGGTGAGCGGATCGAGGTGCGCGGCCGCAGCGCACGCGGCGAGGTGCGCGAAACCGGCCGCAACCGCGGCAACGA
CGACGCATTCCGCCGCACGCACACCGACGCGCCCGACAGCGAGTTCTTCAACGCGCTCGGCGATGCGCTGAAGCAGCGCT
TCGCGGGCTGGCAGGCGTTCCTGCTGACGTCGGACCGCGCGCTGCCCGGCCAGCTGCGGCTGCGCGAATCGGCGAAGACG
CCGCTGTTCAACGGCGCGCTCGAATGCAGGTTGTTCCGTTTCGACCTGATCGCCGGCAGCGTGAAGGCGCGCCCGGCCGC
ACCGGACGGCGACGCGTAA

Upstream 100 bases:

>100_bases
CATGGCGTGCCGGCCGCGAACCGCGTTGCGGCCGACGCCGGGCCGCACGCGCCGGCGCCGCGTACAATACTGGACTTTTG
TACGTCCCCGCACCCGCCTT

Downstream 100 bases:

>100_bases
GCGTCGTCGCCACCCGCACCGGCGCCGCGCGCGCCGGCTGCTGCCACCCACCGCCATGCCCGGCCGCCCGGCCGGGTCCC
TTCCCCGTCCCGCTCCCCGC

Product: putative RNA methylase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MSSPTLYDFFAPCPRGLEAALAAELAEIAGRHLDGAPFAAGAQVPGGVHFRGGWAAGMAANLHSRIASRILLKIAHRPYR
SEQDVYALALEQPWERWFAATQTLRVDITAIKSPLKSLEFATLRVKDAICDRMRDKTGARPSIDTGAPDVRVFAFLTATD
CTLYLDTSGEPLFKRGWRLDKGAAPLRENLAAGILRLTGWTPGTPLYDPMCGSGTFLAEAAQMVLGVAPGVERRFGFEKL
KQYDITAWQGLKVAALDAKRAARGRRDDLRVYGSDISGDMLEKARANLERAGVPSVWLKQVDARGMTPPCDGPGLIVANP
PYGERIEVRGRSARGEVRETGRNRGNDDAFRRTHTDAPDSEFFNALGDALKQRFAGWQAFLLTSDRALPGQLRLRESAKT
PLFNGALECRLFRFDLIAGSVKARPAAPDGDA

Sequences:

>Translated_432_residues
MSSPTLYDFFAPCPRGLEAALAAELAEIAGRHLDGAPFAAGAQVPGGVHFRGGWAAGMAANLHSRIASRILLKIAHRPYR
SEQDVYALALEQPWERWFAATQTLRVDITAIKSPLKSLEFATLRVKDAICDRMRDKTGARPSIDTGAPDVRVFAFLTATD
CTLYLDTSGEPLFKRGWRLDKGAAPLRENLAAGILRLTGWTPGTPLYDPMCGSGTFLAEAAQMVLGVAPGVERRFGFEKL
KQYDITAWQGLKVAALDAKRAARGRRDDLRVYGSDISGDMLEKARANLERAGVPSVWLKQVDARGMTPPCDGPGLIVANP
PYGERIEVRGRSARGEVRETGRNRGNDDAFRRTHTDAPDSEFFNALGDALKQRFAGWQAFLLTSDRALPGQLRLRESAKT
PLFNGALECRLFRFDLIAGSVKARPAAPDGDA
>Mature_431_residues
SSPTLYDFFAPCPRGLEAALAAELAEIAGRHLDGAPFAAGAQVPGGVHFRGGWAAGMAANLHSRIASRILLKIAHRPYRS
EQDVYALALEQPWERWFAATQTLRVDITAIKSPLKSLEFATLRVKDAICDRMRDKTGARPSIDTGAPDVRVFAFLTATDC
TLYLDTSGEPLFKRGWRLDKGAAPLRENLAAGILRLTGWTPGTPLYDPMCGSGTFLAEAAQMVLGVAPGVERRFGFEKLK
QYDITAWQGLKVAALDAKRAARGRRDDLRVYGSDISGDMLEKARANLERAGVPSVWLKQVDARGMTPPCDGPGLIVANPP
YGERIEVRGRSARGEVRETGRNRGNDDAFRRTHTDAPDSEFFNALGDALKQRFAGWQAFLLTSDRALPGQLRLRESAKTP
LFNGALECRLFRFDLIAGSVKARPAAPDGDA

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=388, Percent_Identity=33.5051546391753, Blast_Score=197, Evalue=9e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 47109; Mature: 46978

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: PS51165 THUMP ; PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPTLYDFFAPCPRGLEAALAAELAEIAGRHLDGAPFAAGAQVPGGVHFRGGWAAGMAA
CCCCCCCHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCHHHHHHH
NLHSRIASRILLKIAHRPYRSEQDVYALALEQPWERWFAATQTLRVDITAIKSPLKSLEF
HHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHH
ATLRVKDAICDRMRDKTGARPSIDTGAPDVRVFAFLTATDCTLYLDTSGEPLFKRGWRLD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCEEEEEECCCCHHHHCCCCCC
KGAAPLRENLAAGILRLTGWTPGTPLYDPMCGSGTFLAEAAQMVLGVAPGVERRFGFEKL
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHCHHHH
KQYDITAWQGLKVAALDAKRAARGRRDDLRVYGSDISGDMLEKARANLERAGVPSVWLKQ
HHHCCCHHCCCEEEEECHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHH
VDARGMTPPCDGPGLIVANPPYGERIEVRGRSARGEVRETGRNRGNDDAFRRTHTDAPDS
HHCCCCCCCCCCCEEEEECCCCCCEEEECCCCCCCHHHHHCCCCCCCHHHHHCCCCCCHH
EFFNALGDALKQRFAGWQAFLLTSDRALPGQLRLRESAKTPLFNGALECRLFRFDLIAGS
HHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHEEHHHCCCCCCCCCCEEEEEEEEEHCCC
VKARPAAPDGDA
CCCCCCCCCCCC
>Mature Secondary Structure 
SSPTLYDFFAPCPRGLEAALAAELAEIAGRHLDGAPFAAGAQVPGGVHFRGGWAAGMAA
CCCCCCHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCHHHHHHH
NLHSRIASRILLKIAHRPYRSEQDVYALALEQPWERWFAATQTLRVDITAIKSPLKSLEF
HHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHH
ATLRVKDAICDRMRDKTGARPSIDTGAPDVRVFAFLTATDCTLYLDTSGEPLFKRGWRLD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCEEEEEECCCCHHHHCCCCCC
KGAAPLRENLAAGILRLTGWTPGTPLYDPMCGSGTFLAEAAQMVLGVAPGVERRFGFEKL
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHCHHHH
KQYDITAWQGLKVAALDAKRAARGRRDDLRVYGSDISGDMLEKARANLERAGVPSVWLKQ
HHHCCCHHCCCEEEEECHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHH
VDARGMTPPCDGPGLIVANPPYGERIEVRGRSARGEVRETGRNRGNDDAFRRTHTDAPDS
HHCCCCCCCCCCCEEEEECCCCCCEEEECCCCCCCHHHHHCCCCCCCHHHHHCCCCCCHH
EFFNALGDALKQRFAGWQAFLLTSDRALPGQLRLRESAKTPLFNGALECRLFRFDLIAGS
HHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHEEHHHCCCCCCCCCCEEEEEEEEEHCCC
VKARPAAPDGDA
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA