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The map label for this gene is citH [H]

Identifier: 161525837

GI number: 161525837

Start: 2914650

End: 2915927

Strand: Reverse

Name: citH [H]

Synonym: Bmul_2668

Alternate gene names: 161525837

Gene position: 2915927-2914650 (Counterclockwise)

Preceding gene: 161525838

Following gene: 161525831

Centisome position: 84.56

GC content: 68.7

Gene sequence:

>1278_bases
ATGCATGCCGTCACGCCGTCCCGTTCCATTGCCTCGTCGCCGCGCGTGTGGCGCGCGGTGGTCGCCGCGTCGATCGGCAA
CGCGCTCGAGTGGTTCGACCTCGTCGTCTACGGCTTCTTCGCGGTGACGATTTCCAGGCTGTTCTTTCCGGCCGGCAACG
ATACCGTGTCGCTGTTGCTTACGCTCGGCACGTTCGGCGTGTCGTTCTTCATGCGTCCGCTCGGCGCGATCGTGCTCGGC
GCCTATGCGGACCGCGCGGGCCGCAAGGCCGCGCTCACGCTGTCGATCCTGCTGATGATGGCGGGCACGCTGATCATCGC
GGTGCTGCCGACCTACGAGACGATCGGCATCGCGGCGCCGCTGATCCTCGTCGCCGCGCGGCTGATGCAGGGCTTTTCGG
CCGGCGGCGAGTTCGGCAGCGCGACCGCGTTCCTCGCCGAGCACGTGCCGGGGCGGCGCGGCTTCTTCGCGAGCTGGCAG
GTCGCGAGCCAGGGCCTGACGACGCTGCTCGCGGCCGGCTTCGGCACCGTGCTGAACGCGCAGCTGACGGCCGCGCAGAT
GGCCGCGTGGGGCTGGCGCGTGCCGTTCTTCTTCGGGCTGCTGCTGGGGCCGGTTGCTTACTACATCCGCAAGAAGGTCG
ACGAGACGCCGGAGTTCCTCGCGGCCGAAAGCACCGCGAGCCCGCTGCGCGACACGTTCAAGTCGCACAAGGCGCGGCTC
GTCGCCGCGATCGGCGCGGTGGTGCTCGGCACGGTGGCGACGTATCTCGTGCTGTTCATGCCGACCTACGGCGTGAAGCA
GCTCGGCCTCGCGCCGTCGGCCGCGTTTGCGGCGATTCTCGTCGTCGGCGTGATCCAGATGGCGTTCGCGCCGCTCGTCG
GCCACTGGTCGGACCGTTACGGCCGCGTGCGCGTGATGCTCGCGCCGGCGCTCGGCTTCCTCGTGCTGATCTACCCGGCA
TTCGCGTATCTGGTCGCGCATCCGGGCTTCGGCACGCTGATTGCGCTGCAGGTGCTGCTTGCCGTGCTGATGACGGGCTA
TTTCGCGGCGCTGCCGGGCCTGTTGTCCGAGGTGTTTCCGGTACAGACGCGCACGACCGGGATGTCGCTCGCGTACAACA
TCGCGGTGACGATCTTCGGCGGCTTCGGGCCGTTCATCATCGCGTGGCTCATCAAGGCGACCGGGATGAAGACCGCGCCG
AGCTTCTACCTGATGTTCGCGGCGGTGCTGAGCCTCGCGGCGCTGGTCGTGTTGCGGCGGCGGTTCGGGTTTCGGTGA

Upstream 100 bases:

>100_bases
TTTTTGCGCGCCTATAATCGTTCGGGCGCCTGCCGGCGCGGCGGCCGACCGCGTACGCGGCGGCGCGCGAACAGAACGTA
CAACGACAAGGAGATCGCCG

Downstream 100 bases:

>100_bases
CGGGGCGCCGTGGGCGCGGCGCCAGCAAGAAGGGGCGGCGGCCGTGCAGTGTTGCGCTCCTTGCGGTAAGCGGACGGGGA
AGGGGAGCGCTTCAGCCTAC

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MHAVTPSRSIASSPRVWRAVVAASIGNALEWFDLVVYGFFAVTISRLFFPAGNDTVSLLLTLGTFGVSFFMRPLGAIVLG
AYADRAGRKAALTLSILLMMAGTLIIAVLPTYETIGIAAPLILVAARLMQGFSAGGEFGSATAFLAEHVPGRRGFFASWQ
VASQGLTTLLAAGFGTVLNAQLTAAQMAAWGWRVPFFFGLLLGPVAYYIRKKVDETPEFLAAESTASPLRDTFKSHKARL
VAAIGAVVLGTVATYLVLFMPTYGVKQLGLAPSAAFAAILVVGVIQMAFAPLVGHWSDRYGRVRVMLAPALGFLVLIYPA
FAYLVAHPGFGTLIALQVLLAVLMTGYFAALPGLLSEVFPVQTRTTGMSLAYNIAVTIFGGFGPFIIAWLIKATGMKTAP
SFYLMFAAVLSLAALVVLRRRFGFR

Sequences:

>Translated_425_residues
MHAVTPSRSIASSPRVWRAVVAASIGNALEWFDLVVYGFFAVTISRLFFPAGNDTVSLLLTLGTFGVSFFMRPLGAIVLG
AYADRAGRKAALTLSILLMMAGTLIIAVLPTYETIGIAAPLILVAARLMQGFSAGGEFGSATAFLAEHVPGRRGFFASWQ
VASQGLTTLLAAGFGTVLNAQLTAAQMAAWGWRVPFFFGLLLGPVAYYIRKKVDETPEFLAAESTASPLRDTFKSHKARL
VAAIGAVVLGTVATYLVLFMPTYGVKQLGLAPSAAFAAILVVGVIQMAFAPLVGHWSDRYGRVRVMLAPALGFLVLIYPA
FAYLVAHPGFGTLIALQVLLAVLMTGYFAALPGLLSEVFPVQTRTTGMSLAYNIAVTIFGGFGPFIIAWLIKATGMKTAP
SFYLMFAAVLSLAALVVLRRRFGFR
>Mature_425_residues
MHAVTPSRSIASSPRVWRAVVAASIGNALEWFDLVVYGFFAVTISRLFFPAGNDTVSLLLTLGTFGVSFFMRPLGAIVLG
AYADRAGRKAALTLSILLMMAGTLIIAVLPTYETIGIAAPLILVAARLMQGFSAGGEFGSATAFLAEHVPGRRGFFASWQ
VASQGLTTLLAAGFGTVLNAQLTAAQMAAWGWRVPFFFGLLLGPVAYYIRKKVDETPEFLAAESTASPLRDTFKSHKARL
VAAIGAVVLGTVATYLVLFMPTYGVKQLGLAPSAAFAAILVVGVIQMAFAPLVGHWSDRYGRVRVMLAPALGFLVLIYPA
FAYLVAHPGFGTLIALQVLLAVLMTGYFAALPGLLSEVFPVQTRTTGMSLAYNIAVTIFGGFGPFIIAWLIKATGMKTAP
SFYLMFAAVLSLAALVVLRRRFGFR

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=427, Percent_Identity=33.2552693208431, Blast_Score=240, Evalue=1e-64,
Organism=Escherichia coli, GI1788942, Length=436, Percent_Identity=36.697247706422, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI1788292, Length=397, Percent_Identity=31.2342569269521, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI1789941, Length=427, Percent_Identity=33.7236533957845, Blast_Score=157, Evalue=9e-40,
Organism=Escherichia coli, GI87082231, Length=457, Percent_Identity=25.601750547046, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI87082404, Length=381, Percent_Identity=24.1469816272966, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 45255; Mature: 45255

Theoretical pI: Translated: 10.77; Mature: 10.77

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHAVTPSRSIASSPRVWRAVVAASIGNALEWFDLVVYGFFAVTISRLFFPAGNDTVSLLL
CCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
TLGTFGVSFFMRPLGAIVLGAYADRAGRKAALTLSILLMMAGTLIIAVLPTYETIGIAAP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LILVAARLMQGFSAGGEFGSATAFLAEHVPGRRGFFASWQVASQGLTTLLAAGFGTVLNA
HHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QLTAAQMAAWGWRVPFFFGLLLGPVAYYIRKKVDETPEFLAAESTASPLRDTFKSHKARL
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHH
VAAIGAVVLGTVATYLVLFMPTYGVKQLGLAPSAAFAAILVVGVIQMAFAPLVGHWSDRY
HHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GRVRVMLAPALGFLVLIYPAFAYLVAHPGFGTLIALQVLLAVLMTGYFAALPGLLSEVFP
CEEEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VQTRTTGMSLAYNIAVTIFGGFGPFIIAWLIKATGMKTAPSFYLMFAAVLSLAALVVLRR
CCCCCCCHHHHEEEEEEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
RFGFR
HHCCC
>Mature Secondary Structure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HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]