Click here to switch to the map view.

The map label for this gene is yafV [C]

Identifier: 161525813

GI number: 161525813

Start: 2885587

End: 2886414

Strand: Direct

Name: yafV [C]

Synonym: Bmul_2644

Alternate gene names: 161525813

Gene position: 2885587-2886414 (Clockwise)

Preceding gene: 161525812

Following gene: 161525814

Centisome position: 83.68

GC content: 69.2

Gene sequence:

>828_bases
ATGACCGATTCCGCCTCATCCGCCACGCCGTTCCGCGTTGCCGCCTTGCAGATGGTCAGCACGCCCGACGTCGGACGCAA
TCTCGCCGAAGCGCGCCGCCTGATCGCGGAAGCCGCCGACGAAGGCGCGCAGCTCGTGCTGCTGCCCGAGTATTTCTGCT
TCATGGGGCAGCGCGACACCGACAAGCTCGCGCTCGCCGAGCCGTACGGCGACGGCCCGATCCAGCAATTCCTCGCCGAC
GCCGCACGCCGCCACGCGCTCTGGGTCATCGGCGGCACGCTGCCGCTGAAGGCGCCGGAACCGCAGCGCGTGCTGAACAC
GACGCTCGTGTTCGATCCGTCGGGCAACGAGGCCGCGCGCTACGACAAGATCCATCTGTTCAACTTCGAGAAAGGCGACG
AGTCGTTCGACGAGGCGCGCACGATCCGCCCGGGCGAGACGGTCGTCACGTTCGACGCGCCGTTCGGCCGGGTCGGGCTG
TCGGTCTGTTACGATCTGCGCTTTCCGGAGCTGTATCGCAGGATGGGCGATTGCGCGCTGATCGTCGTGCCGTCGGCGTT
TACGTATACGACCGGCCGCGCGCACTGGGAGACGCTGCTGCGCGCGCGGGCGGTCGAGAACCAGTGCTACGTGCTCGCGG
CCGCACAGGGCGGCAAGCACGAGAACGGCCGACGCACCTGGGGCCACAGCATGCTGATCGACCCGTGGGGCGAGATCGTC
GCGGTACGCGACGAAGGCGCGAGCGTCGTGAGCGGCACGCTCGACGCGCAGCGCATCGCCGACGTGCGCCAGAGCCTGCC
CGCGTGGCGGCACCGCGTACTGACCTGA

Upstream 100 bases:

>100_bases
CGATCAGGGTAAGATGAATCGCGATGCGGCCGAGGCGGCCGGCCACTGAGCCGCCGTTACCGCCGGGCCGCACCCCATTT
ATGACGCCACCGATTTCGCG

Downstream 100 bases:

>100_bases
GCGGCCGGTCCGCTTGAAACGCCGCGCGGCCCACCCATCTGCGCGATACGCATCCGACAACTTTTTGAGAGCACCCGATA
CCCGCATGAACATCATCGAA

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MTDSASSATPFRVAALQMVSTPDVGRNLAEARRLIAEAADEGAQLVLLPEYFCFMGQRDTDKLALAEPYGDGPIQQFLAD
AARRHALWVIGGTLPLKAPEPQRVLNTTLVFDPSGNEAARYDKIHLFNFEKGDESFDEARTIRPGETVVTFDAPFGRVGL
SVCYDLRFPELYRRMGDCALIVVPSAFTYTTGRAHWETLLRARAVENQCYVLAAAQGGKHENGRRTWGHSMLIDPWGEIV
AVRDEGASVVSGTLDAQRIADVRQSLPAWRHRVLT

Sequences:

>Translated_275_residues
MTDSASSATPFRVAALQMVSTPDVGRNLAEARRLIAEAADEGAQLVLLPEYFCFMGQRDTDKLALAEPYGDGPIQQFLAD
AARRHALWVIGGTLPLKAPEPQRVLNTTLVFDPSGNEAARYDKIHLFNFEKGDESFDEARTIRPGETVVTFDAPFGRVGL
SVCYDLRFPELYRRMGDCALIVVPSAFTYTTGRAHWETLLRARAVENQCYVLAAAQGGKHENGRRTWGHSMLIDPWGEIV
AVRDEGASVVSGTLDAQRIADVRQSLPAWRHRVLT
>Mature_274_residues
TDSASSATPFRVAALQMVSTPDVGRNLAEARRLIAEAADEGAQLVLLPEYFCFMGQRDTDKLALAEPYGDGPIQQFLADA
ARRHALWVIGGTLPLKAPEPQRVLNTTLVFDPSGNEAARYDKIHLFNFEKGDESFDEARTIRPGETVVTFDAPFGRVGLS
VCYDLRFPELYRRMGDCALIVVPSAFTYTTGRAHWETLLRARAVENQCYVLAAAQGGKHENGRRTWGHSMLIDPWGEIVA
VRDEGASVVSGTLDAQRIADVRQSLPAWRHRVLT

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=271, Percent_Identity=38.7453874538745, Blast_Score=194, Evalue=9e-50,
Organism=Homo sapiens, GI297632350, Length=279, Percent_Identity=37.9928315412186, Blast_Score=190, Evalue=1e-48,
Organism=Homo sapiens, GI5031947, Length=279, Percent_Identity=37.9928315412186, Blast_Score=189, Evalue=2e-48,
Organism=Homo sapiens, GI297632348, Length=279, Percent_Identity=37.9928315412186, Blast_Score=189, Evalue=2e-48,
Organism=Homo sapiens, GI297632346, Length=204, Percent_Identity=34.3137254901961, Blast_Score=109, Evalue=2e-24,
Organism=Escherichia coli, GI1786412, Length=230, Percent_Identity=30.8695652173913, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17556280, Length=265, Percent_Identity=40.377358490566, Blast_Score=199, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6323383, Length=283, Percent_Identity=34.982332155477, Blast_Score=175, Evalue=9e-45,
Organism=Saccharomyces cerevisiae, GI6322335, Length=299, Percent_Identity=34.4481605351171, Blast_Score=166, Evalue=3e-42,
Organism=Drosophila melanogaster, GI17933642, Length=266, Percent_Identity=36.0902255639098, Blast_Score=182, Evalue=2e-46,
Organism=Drosophila melanogaster, GI21355835, Length=276, Percent_Identity=32.9710144927536, Blast_Score=160, Evalue=7e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 30405; Mature: 30274

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDSASSATPFRVAALQMVSTPDVGRNLAEARRLIAEAADEGAQLVLLPEYFCFMGQRDT
CCCCCCCCCCHHEEEEEHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHCCCCCC
DKLALAEPYGDGPIQQFLADAARRHALWVIGGTLPLKAPEPQRVLNTTLVFDPSGNEAAR
CCEEEECCCCCCHHHHHHHHHHHHCEEEEEECEECCCCCCHHHHEEEEEEECCCCCCCCC
YDKIHLFNFEKGDESFDEARTIRPGETVVTFDAPFGRVGLSVCYDLRFPELYRRMGDCAL
CCEEEEEECCCCCCCHHHHHCCCCCCEEEEECCCCCHHHHHHHEECCCHHHHHHHCCEEE
IVVPSAFTYTTGRAHWETLLRARAVENQCYVLAAAQGGKHENGRRTWGHSMLIDPWGEIV
EEECCCEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEEECCCCCEE
AVRDEGASVVSGTLDAQRIADVRQSLPAWRHRVLT
EEECCCCEEEECCCCHHHHHHHHHHCHHHHHHCCC
>Mature Secondary Structure 
TDSASSATPFRVAALQMVSTPDVGRNLAEARRLIAEAADEGAQLVLLPEYFCFMGQRDT
CCCCCCCCCHHEEEEEHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHCCCCCC
DKLALAEPYGDGPIQQFLADAARRHALWVIGGTLPLKAPEPQRVLNTTLVFDPSGNEAAR
CCEEEECCCCCCHHHHHHHHHHHHCEEEEEECEECCCCCCHHHHEEEEEEECCCCCCCCC
YDKIHLFNFEKGDESFDEARTIRPGETVVTFDAPFGRVGLSVCYDLRFPELYRRMGDCAL
CCEEEEEECCCCCCCHHHHHCCCCCCEEEEECCCCCHHHHHHHEECCCHHHHHHHCCEEE
IVVPSAFTYTTGRAHWETLLRARAVENQCYVLAAAQGGKHENGRRTWGHSMLIDPWGEIV
EEECCCEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEEECCCCCEE
AVRDEGASVVSGTLDAQRIADVRQSLPAWRHRVLT
EEECCCCEEEECCCCHHHHHHHHHHCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]