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The map label for this gene is pcnB [H]

Identifier: 161525796

GI number: 161525796

Start: 2862118

End: 2863656

Strand: Direct

Name: pcnB [H]

Synonym: Bmul_2627

Alternate gene names: 161525796

Gene position: 2862118-2863656 (Clockwise)

Preceding gene: 161525795

Following gene: 161525797

Centisome position: 83.0

GC content: 69.27

Gene sequence:

>1539_bases
GTGATCAAGAAACTCATCCGCAAGCTGCTCGGCCAGGACGACGCCGTGCAAACCTCCCCCGCCGCGAGCGCGGCCGACAC
ACCGGCTTCCGCCGCGCCGCGGCCGGCCAGATCCGCGCGCGCCGCCGCCGCGAAAAAGCCGCGCAGCAATCACGAGCCGA
CCATCGTGCCGGCCAGCGTGCACGGGATCGATCCCGCGCTGATCTCGAAGAACGCCGTGCGCGTGACCGACACGCTGCAG
CAGGCCGGTTACCGCGCGTTCATCGTCGGCGGCGCAGTGCGCGACCTGCTGCTCGGGATCGCGCCGAAGGATTTCGACGT
CGCGACCGATGCGACACCGACCGAAGTGCAGCGCCTGTTCCGCCGTGCGCGCCTGATCGGCCGCCGCTTCCAGATCGTCC
ACGTGCAGTTCGGCCAGGAGCTGATCGAGGTGTCGACGTTCCGCGCGCTCGTCGATGCGCCGCCGGAAGCCGCGACGGCC
GAGCCGCCCAAGCGCCTGAAGCGCGACGAGCTCGATCGCCGCACGCATGCGGTCGACGCGAGCGGCCGCGTGCTGCGCGA
CAACGTCTGGGGCGAGCAGCACGAAGACGCCGCGCGCCGCGACTTCACGATCAACGCGATGTACTACGACCCGTCGACGC
AGACGGTGCTCGACTACCACGACGGCATGGCCGACGTGCGTGCGCGCCTGCTGCGGATGATCGGCGATCCGGCGACGCGC
TACCGCGAGGATCCGGTGCGGATGCTGCGCGTCGTGCGTTTCGCGGCGAAGCTCGGCTTCGAGATCGAGCCGCACACGCG
CGAGCCCATCAACGCGCTCGCCGATCTCATCAACAACGTGCCGGCCGCGCGCCTCTTCGACGAGATGCTGAAGCTGCTGC
TGTCGGGCCATGCGCTCGCGTGCCTGCAGCGGCTGCGCAAGGAAGGGCTGCACCACGGGCTGCTGCCGCTGCTCGACGTC
GTGCTCGAACAGCCGCAGGGCGAGAAGTTCATCACGCTCGCGCTGAACAATACGGACGCGCGCGTGCGCGCCGGCAAGCC
CGTATCGCCGGGCTTCCTGTTCGCGACGCTGCTGTGGCACGACATGCGCCAGCGCTTCGAGCAGTACACGGCCGAAGGCG
AATTCCCGGTGCCCGCGCTGCACCGCGCGATGGACGACGTGCTCGACATGCAGACCGAAAAGCTGGCGATCCACAAGCGC
TATTCGGCCGACATGCGCGAGATCTGGGGCCTGCAGCTGCGCCTCGAAAAACGCTCGGGCCGCAGCGCGCTGCGGCTGCT
GGAACACCAAAGATTTAGAGCGGGGTATGATTTCCTCCTGTTACGCTGCGAATCCGGCGAGCTCGACCCGGCTGTCGGAC
AGTGGTGGACGGATTTCATCGAAGGCGACGCGACCGCGCGCGAGGCACTGCTCTCGCAGGGCGGCTCGAAGGAGAAATCG
CCCCGCAAGCGGCGCCGCCGCGGCGGTGCGCGCAACCGCAAGTCGGGCGAAGGCGCCGCCGAGCAGGCGCCCGATACCGC
GGGCAGCTCCGACGACTGA

Upstream 100 bases:

>100_bases
TCGAGAAAGTCACCGATCCGATCGCGACCAATCCCGACGACACGCTGCGCGCGCATGCCCGCGACCGCGGCTGGCGCATC
CTCGATCTCTTCTGAACGTC

Downstream 100 bases:

>100_bases
CGCGCTCGCCGGCGAACGACGCAGGAAGTGATGCCATGACGGTTGCATATCTCGGGCTCGGCGCAAATCTCGGCGATGCG
CGCCAGACCCTGAAGGACGC

Product: poly(A) polymerase

Products: NA

Alternate protein names: PAP [H]

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MIKKLIRKLLGQDDAVQTSPAASAADTPASAAPRPARSARAAAAKKPRSNHEPTIVPASVHGIDPALISKNAVRVTDTLQ
QAGYRAFIVGGAVRDLLLGIAPKDFDVATDATPTEVQRLFRRARLIGRRFQIVHVQFGQELIEVSTFRALVDAPPEAATA
EPPKRLKRDELDRRTHAVDASGRVLRDNVWGEQHEDAARRDFTINAMYYDPSTQTVLDYHDGMADVRARLLRMIGDPATR
YREDPVRMLRVVRFAAKLGFEIEPHTREPINALADLINNVPAARLFDEMLKLLLSGHALACLQRLRKEGLHHGLLPLLDV
VLEQPQGEKFITLALNNTDARVRAGKPVSPGFLFATLLWHDMRQRFEQYTAEGEFPVPALHRAMDDVLDMQTEKLAIHKR
YSADMREIWGLQLRLEKRSGRSALRLLEHQRFRAGYDFLLLRCESGELDPAVGQWWTDFIEGDATAREALLSQGGSKEKS
PRKRRRRGGARNRKSGEGAAEQAPDTAGSSDD

Sequences:

>Translated_512_residues
MIKKLIRKLLGQDDAVQTSPAASAADTPASAAPRPARSARAAAAKKPRSNHEPTIVPASVHGIDPALISKNAVRVTDTLQ
QAGYRAFIVGGAVRDLLLGIAPKDFDVATDATPTEVQRLFRRARLIGRRFQIVHVQFGQELIEVSTFRALVDAPPEAATA
EPPKRLKRDELDRRTHAVDASGRVLRDNVWGEQHEDAARRDFTINAMYYDPSTQTVLDYHDGMADVRARLLRMIGDPATR
YREDPVRMLRVVRFAAKLGFEIEPHTREPINALADLINNVPAARLFDEMLKLLLSGHALACLQRLRKEGLHHGLLPLLDV
VLEQPQGEKFITLALNNTDARVRAGKPVSPGFLFATLLWHDMRQRFEQYTAEGEFPVPALHRAMDDVLDMQTEKLAIHKR
YSADMREIWGLQLRLEKRSGRSALRLLEHQRFRAGYDFLLLRCESGELDPAVGQWWTDFIEGDATAREALLSQGGSKEKS
PRKRRRRGGARNRKSGEGAAEQAPDTAGSSDD
>Mature_512_residues
MIKKLIRKLLGQDDAVQTSPAASAADTPASAAPRPARSARAAAAKKPRSNHEPTIVPASVHGIDPALISKNAVRVTDTLQ
QAGYRAFIVGGAVRDLLLGIAPKDFDVATDATPTEVQRLFRRARLIGRRFQIVHVQFGQELIEVSTFRALVDAPPEAATA
EPPKRLKRDELDRRTHAVDASGRVLRDNVWGEQHEDAARRDFTINAMYYDPSTQTVLDYHDGMADVRARLLRMIGDPATR
YREDPVRMLRVVRFAAKLGFEIEPHTREPINALADLINNVPAARLFDEMLKLLLSGHALACLQRLRKEGLHHGLLPLLDV
VLEQPQGEKFITLALNNTDARVRAGKPVSPGFLFATLLWHDMRQRFEQYTAEGEFPVPALHRAMDDVLDMQTEKLAIHKR
YSADMREIWGLQLRLEKRSGRSALRLLEHQRFRAGYDFLLLRCESGELDPAVGQWWTDFIEGDATAREALLSQGGSKEKS
PRKRRRRGGARNRKSGEGAAEQAPDTAGSSDD

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=243, Percent_Identity=28.8065843621399, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI87081691, Length=433, Percent_Identity=44.1108545034642, Blast_Score=342, Evalue=3e-95,
Organism=Escherichia coli, GI1789436, Length=253, Percent_Identity=29.6442687747036, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281360112, Length=241, Percent_Identity=28.6307053941909, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21357337, Length=241, Percent_Identity=28.6307053941909, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 57168; Mature: 57168

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKLIRKLLGQDDAVQTSPAASAADTPASAAPRPARSARAAAAKKPRSNHEPTIVPASV
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEECCC
HGIDPALISKNAVRVTDTLQQAGYRAFIVGGAVRDLLLGIAPKDFDVATDATPTEVQRLF
CCCCHHHHCCCCHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
RRARLIGRRFQIVHVQFGQELIEVSTFRALVDAPPEAATAEPPKRLKRDELDRRTHAVDA
HHHHHHCCHHEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
SGRVLRDNVWGEQHEDAARRDFTINAMYYDPSTQTVLDYHDGMADVRARLLRMIGDPATR
CCCEEECCCCCCCHHHHHHCCEEEEEEEECCCCCCHHHHHCCHHHHHHHHHHHHCCHHHH
YREDPVRMLRVVRFAAKLGFEIEPHTREPINALADLINNVPAARLFDEMLKLLLSGHALA
HHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHH
CLQRLRKEGLHHGLLPLLDVVLEQPQGEKFITLALNNTDARVRAGKPVSPGFLFATLLWH
HHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCEEECCCCCCCHHHHHHHHHH
DMRQRFEQYTAEGEFPVPALHRAMDDVLDMQTEKLAIHKRYSADMREIWGLQLRLEKRSG
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCC
RSALRLLEHQRFRAGYDFLLLRCESGELDPAVGQWWTDFIEGDATAREALLSQGGSKEKS
HHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCC
PRKRRRRGGARNRKSGEGAAEQAPDTAGSSDD
HHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCC
>Mature Secondary Structure
MIKKLIRKLLGQDDAVQTSPAASAADTPASAAPRPARSARAAAAKKPRSNHEPTIVPASV
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEECCC
HGIDPALISKNAVRVTDTLQQAGYRAFIVGGAVRDLLLGIAPKDFDVATDATPTEVQRLF
CCCCHHHHCCCCHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
RRARLIGRRFQIVHVQFGQELIEVSTFRALVDAPPEAATAEPPKRLKRDELDRRTHAVDA
HHHHHHCCHHEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
SGRVLRDNVWGEQHEDAARRDFTINAMYYDPSTQTVLDYHDGMADVRARLLRMIGDPATR
CCCEEECCCCCCCHHHHHHCCEEEEEEEECCCCCCHHHHHCCHHHHHHHHHHHHCCHHHH
YREDPVRMLRVVRFAAKLGFEIEPHTREPINALADLINNVPAARLFDEMLKLLLSGHALA
HHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHH
CLQRLRKEGLHHGLLPLLDVVLEQPQGEKFITLALNNTDARVRAGKPVSPGFLFATLLWH
HHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCEEECCCCCCCHHHHHHHHHH
DMRQRFEQYTAEGEFPVPALHRAMDDVLDMQTEKLAIHKRYSADMREIWGLQLRLEKRSG
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCC
RSALRLLEHQRFRAGYDFLLLRCESGELDPAVGQWWTDFIEGDATAREALLSQGGSKEKS
HHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCC
PRKRRRRGGARNRKSGEGAAEQAPDTAGSSDD
HHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]