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The map label for this gene is miaA

Identifier: 161525791

GI number: 161525791

Start: 2857828

End: 2858805

Strand: Direct

Name: miaA

Synonym: Bmul_2622

Alternate gene names: 161525791

Gene position: 2857828-2858805 (Clockwise)

Preceding gene: 161525790

Following gene: 161525794

Centisome position: 82.87

GC content: 71.06

Gene sequence:

>978_bases
ATGAGCGTGTCCGCGCAGTCCGTCCCGACGACGATCGCCTGTCTGCTCGGCCCCACCGCATCGGGCAAGACGGCCGCTGC
GCTGGCGCTCGCCGCGCGCCGGCCGATCGAGATCGTCAGCGTCGATTCGGCGCTCGTCTACCGCGACATGGACATCGGCA
CCGCGAAACCGACGCGCGAGGAGCGCGCCCGCGTGCCGCACCATCTGATCGACATCATCGACCCGGCCGACGCGTATTCG
GCGGCCAGCTTCCGCGCGGACACGCTGCGGCTGATCGGCGAGATCGTCGCGCGCGGCAACACGCCGCTGCTCGCGGGCGG
CACGATGCTGTACTACAAGGCGCTCACGCAAGGGCTGAACGATCTGCCGACGGCCGATCCCGCGGTGCGCGCGGAACTCG
ACGCCGAAGCCGCGCGCGACGGCTGGCCCGCGCTGCATGCGCGGCTCGCGCAGATCGATCCGGCAACGGCCGCGCGGCTC
GCGCCGAACGACGCGCAGCGCATTCAGCGCGCGCTCGAAGTCTTCATGCTGAGCGGGCAGCCGATGTCCGCGCTGCTCGC
GGCGCCGCGTCCCGCCGACGAAGCGGCGGCCGCCTACCGCTTCGTGCCGGTCGCGCTCGAACCGTCCGATCGCGCGGTGC
TGCACGCCCGCATCGCGCAGCGCTTCGACGCGATGCTCGCCGCGGGCTTCATCGACGAAGTGGAACGACTGCGCCGCCGC
GACGATCTGCATCTCGGCCTGCCGTCGATGCGCTGCGTCGGCTATCGGCAGGCATGGGAATTCCTCGACGGCGACATCGA
TTACCGGACGATGCGCGACAAGGGCATCTTCGCGACGCGGCAGCTGTGCAAACGGCAGATCACGTGGCTGCGCGCGATGC
CGGAACGGATCGTCGTCGATTGCATCGCGCCCGATTCGACCGCGCGCGCGCTCGACGCGCTCGAACGCGTGCTCGACGGG
CGTATTGCCGCGGGCTGA

Upstream 100 bases:

>100_bases
AGATGGAAGCGACCGAACGCGCGGACCAGTGCAATCACGGCCGGCCGACCTGGTATCAGCTCACGCTGAGCGATCTCGAC
CGCCTGTTCATGCGCGGCCA

Downstream 100 bases:

>100_bases
GCCGGACGACATCGCCGGGCCGCGCGCCGCGACGGCACCCCTACGCGGCGACCCGCGCCGCGCAGCCGGAACACGCCTGC
ACCGTCGCCGCGCTCATTGC

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MSVSAQSVPTTIACLLGPTASGKTAAALALAARRPIEIVSVDSALVYRDMDIGTAKPTREERARVPHHLIDIIDPADAYS
AASFRADTLRLIGEIVARGNTPLLAGGTMLYYKALTQGLNDLPTADPAVRAELDAEAARDGWPALHARLAQIDPATAARL
APNDAQRIQRALEVFMLSGQPMSALLAAPRPADEAAAAYRFVPVALEPSDRAVLHARIAQRFDAMLAAGFIDEVERLRRR
DDLHLGLPSMRCVGYRQAWEFLDGDIDYRTMRDKGIFATRQLCKRQITWLRAMPERIVVDCIAPDSTARALDALERVLDG
RIAAG

Sequences:

>Translated_325_residues
MSVSAQSVPTTIACLLGPTASGKTAAALALAARRPIEIVSVDSALVYRDMDIGTAKPTREERARVPHHLIDIIDPADAYS
AASFRADTLRLIGEIVARGNTPLLAGGTMLYYKALTQGLNDLPTADPAVRAELDAEAARDGWPALHARLAQIDPATAARL
APNDAQRIQRALEVFMLSGQPMSALLAAPRPADEAAAAYRFVPVALEPSDRAVLHARIAQRFDAMLAAGFIDEVERLRRR
DDLHLGLPSMRCVGYRQAWEFLDGDIDYRTMRDKGIFATRQLCKRQITWLRAMPERIVVDCIAPDSTARALDALERVLDG
RIAAG
>Mature_324_residues
SVSAQSVPTTIACLLGPTASGKTAAALALAARRPIEIVSVDSALVYRDMDIGTAKPTREERARVPHHLIDIIDPADAYSA
ASFRADTLRLIGEIVARGNTPLLAGGTMLYYKALTQGLNDLPTADPAVRAELDAEAARDGWPALHARLAQIDPATAARLA
PNDAQRIQRALEVFMLSGQPMSALLAAPRPADEAAAAYRFVPVALEPSDRAVLHARIAQRFDAMLAAGFIDEVERLRRRD
DLHLGLPSMRCVGYRQAWEFLDGDIDYRTMRDKGIFATRQLCKRQITWLRAMPERIVVDCIAPDSTARALDALERVLDGR
IAAG

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=308, Percent_Identity=31.4935064935065, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI1790613, Length=304, Percent_Identity=51.9736842105263, Blast_Score=306, Evalue=1e-84,
Organism=Caenorhabditis elegans, GI25144712, Length=277, Percent_Identity=28.8808664259928, Blast_Score=107, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6324848, Length=299, Percent_Identity=25.752508361204, Blast_Score=126, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24649830, Length=282, Percent_Identity=32.2695035460993, Blast_Score=133, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_BURM1 (A9AGA7)

Other databases:

- EMBL:   CP000868
- EMBL:   AP009385
- RefSeq:   YP_001580803.1
- RefSeq:   YP_001945116.1
- ProteinModelPortal:   A9AGA7
- SMR:   A9AGA7
- GeneID:   5767657
- GeneID:   6357509
- GenomeReviews:   AP009385_GR
- GenomeReviews:   CP000868_GR
- KEGG:   bmj:BMULJ_00616
- KEGG:   bmu:Bmul_2622
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 35326; Mature: 35195

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVSAQSVPTTIACLLGPTASGKTAAALALAARRPIEIVSVDSALVYRDMDIGTAKPTRE
CCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEECCEEEEEECCCCCCCCCHH
ERARVPHHLIDIIDPADAYSAASFRADTLRLIGEIVARGNTPLLAGGTMLYYKALTQGLN
HHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHCCC
DLPTADPAVRAELDAEAARDGWPALHARLAQIDPATAARLAPNDAQRIQRALEVFMLSGQ
CCCCCCCHHHHHCCHHHHCCCCHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHCCCC
PMSALLAAPRPADEAAAAYRFVPVALEPSDRAVLHARIAQRFDAMLAAGFIDEVERLRRR
CHHHHHCCCCCCHHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DDLHLGLPSMRCVGYRQAWEFLDGDIDYRTMRDKGIFATRQLCKRQITWLRAMPERIVVD
CCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHHHHHHHCHHHHEEE
CIAPDSTARALDALERVLDGRIAAG
EECCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SVSAQSVPTTIACLLGPTASGKTAAALALAARRPIEIVSVDSALVYRDMDIGTAKPTRE
CCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEECCEEEEEECCCCCCCCCHH
ERARVPHHLIDIIDPADAYSAASFRADTLRLIGEIVARGNTPLLAGGTMLYYKALTQGLN
HHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHCCC
DLPTADPAVRAELDAEAARDGWPALHARLAQIDPATAARLAPNDAQRIQRALEVFMLSGQ
CCCCCCCHHHHHCCHHHHCCCCHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHCCCC
PMSALLAAPRPADEAAAAYRFVPVALEPSDRAVLHARIAQRFDAMLAAGFIDEVERLRRR
CHHHHHCCCCCCHHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DDLHLGLPSMRCVGYRQAWEFLDGDIDYRTMRDKGIFATRQLCKRQITWLRAMPERIVVD
CCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHHHHHHHCHHHHEEE
CIAPDSTARALDALERVLDGRIAAG
EECCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA