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The map label for this gene is insH10 [H]

Identifier: 161525781

GI number: 161525781

Start: 2843570

End: 2844562

Strand: Reverse

Name: insH10 [H]

Synonym: Bmul_2612

Alternate gene names: 161525781

Gene position: 2844562-2843570 (Counterclockwise)

Preceding gene: 161525782

Following gene: 161525780

Centisome position: 82.49

GC content: 59.01

Gene sequence:

>993_bases
ATGAAGAGGCAGATCGGCTTCGCGGAAGCGGAAATTGCAGGCAAGAAGCGAGTGACGAGGCGCCAACGCTTCCTGGAAGA
GATGGAGAAGGTCGTTCCGTGGCAGCGCTTGCTGTCGGCCATCGAACCGCACTATCCGAAGGGCACGCGAGGTCGCCCGC
CGATTGGCCTTGAGCGGATGCTGCGAATCTACTTCGTGCAACAGTGGTACGGACTGTCGGACGAAGGACTGGAAGACGCG
CTGTATGACAGCATCACGCTGCGAGCCTTCGCCGGCATCGATCTGGCGATCGAGAACGTGCCTGATGCAACCACGCTGTT
GAAGTTCCGGCGCCTGCTGATCGAACACGAACTGACACGGAAGTTGTTCGACGAGATTGGCATCTCGCTGTGCGAGCGTG
GGCTGATGATGAAGGAAGGCACGCTGGTTGACGCGACGATCATTGAAGCGCCGCCGTCGACCAAGAATGCCGGGAAGAGC
CGTGACCCGGAAATGCATCAAACGAAGAAGGGCAACGAATGGCACTTTGGCATGAAAGCCCACATTGGCGTCGACGCCGA
CTCGGGCCTGATTCACAGTGTGGTTGGCACGGCGGCCAACGTGTCGGATGTATCGCAAGCTCATGCCCTGCTGCACGGGC
ATGAAGAGCAGGTGTTCGCCGACGCGGGCTACATTGGCGTCGACAAGCGCGAGGAAATGGCGGGCAAGGCCGTGAAGTGG
CACGTCGCTGCCAGGCGGGGAAAGATCAAGGCGATGCAAGAAGGAGCGCTGAAGGACCTGGTGATCGCGCTCGAGCGAAC
CAAGGCGCAGATCCGTTCGCGGGTTGAGCATCCGTTTCATATCGTCAAGAATCTGTTTCAGCATCGCAAGACCCGATACA
AGGGCTTGGCCAAGAACACCGCGCAACTGTTCAGCCTGTTCGCTCTGGCGAATCTGGTGATTGCGCGAAATCTGTTGCGA
TCGGTCCATGGGAGCAGTCCGTCATGCGTATGA

Upstream 100 bases:

>100_bases
TATCAGACCGTTAAGGAAACGCTGATTTATTCGGCTCGGCGGTCATCGCAGACGTAGCCGAGGACGTTTAGAAGACAGCT
CACGGAACGGACCCACGACG

Downstream 100 bases:

>100_bases
AAAATGCGAGCAGGGGAGGCTCGCTTACGCGCCAAATTCACTGAATTGAGCGCCGATTCGCTTCGTCATCCAGAAAATTT
GAAGCCATCTCGCCTGCAAC

Product: transposase IS4 family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MKRQIGFAEAEIAGKKRVTRRQRFLEEMEKVVPWQRLLSAIEPHYPKGTRGRPPIGLERMLRIYFVQQWYGLSDEGLEDA
LYDSITLRAFAGIDLAIENVPDATTLLKFRRLLIEHELTRKLFDEIGISLCERGLMMKEGTLVDATIIEAPPSTKNAGKS
RDPEMHQTKKGNEWHFGMKAHIGVDADSGLIHSVVGTAANVSDVSQAHALLHGHEEQVFADAGYIGVDKREEMAGKAVKW
HVAARRGKIKAMQEGALKDLVIALERTKAQIRSRVEHPFHIVKNLFQHRKTRYKGLAKNTAQLFSLFALANLVIARNLLR
SVHGSSPSCV

Sequences:

>Translated_330_residues
MKRQIGFAEAEIAGKKRVTRRQRFLEEMEKVVPWQRLLSAIEPHYPKGTRGRPPIGLERMLRIYFVQQWYGLSDEGLEDA
LYDSITLRAFAGIDLAIENVPDATTLLKFRRLLIEHELTRKLFDEIGISLCERGLMMKEGTLVDATIIEAPPSTKNAGKS
RDPEMHQTKKGNEWHFGMKAHIGVDADSGLIHSVVGTAANVSDVSQAHALLHGHEEQVFADAGYIGVDKREEMAGKAVKW
HVAARRGKIKAMQEGALKDLVIALERTKAQIRSRVEHPFHIVKNLFQHRKTRYKGLAKNTAQLFSLFALANLVIARNLLR
SVHGSSPSCV
>Mature_330_residues
MKRQIGFAEAEIAGKKRVTRRQRFLEEMEKVVPWQRLLSAIEPHYPKGTRGRPPIGLERMLRIYFVQQWYGLSDEGLEDA
LYDSITLRAFAGIDLAIENVPDATTLLKFRRLLIEHELTRKLFDEIGISLCERGLMMKEGTLVDATIIEAPPSTKNAGKS
RDPEMHQTKKGNEWHFGMKAHIGVDADSGLIHSVVGTAANVSDVSQAHALLHGHEEQVFADAGYIGVDKREEMAGKAVKW
HVAARRGKIKAMQEGALKDLVIALERTKAQIRSRVEHPFHIVKNLFQHRKTRYKGLAKNTAQLFSLFALANLVIARNLLR
SVHGSSPSCV

Specific function: Involved in the transposition of the insertion sequence IS5 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 11 family [H]

Homologues:

Organism=Escherichia coli, GI1787634, Length=321, Percent_Identity=54.2056074766355, Blast_Score=346, Evalue=1e-96,
Organism=Escherichia coli, GI1789921, Length=321, Percent_Identity=54.2056074766355, Blast_Score=345, Evalue=2e-96,
Organism=Escherichia coli, GI1789612, Length=321, Percent_Identity=54.2056074766355, Blast_Score=345, Evalue=2e-96,
Organism=Escherichia coli, GI1789355, Length=321, Percent_Identity=54.2056074766355, Blast_Score=345, Evalue=2e-96,
Organism=Escherichia coli, GI1788518, Length=321, Percent_Identity=54.2056074766355, Blast_Score=345, Evalue=2e-96,
Organism=Escherichia coli, GI1788342, Length=321, Percent_Identity=54.2056074766355, Blast_Score=345, Evalue=2e-96,
Organism=Escherichia coli, GI1787592, Length=321, Percent_Identity=54.2056074766355, Blast_Score=345, Evalue=2e-96,
Organism=Escherichia coli, GI1786877, Length=321, Percent_Identity=54.2056074766355, Blast_Score=345, Evalue=2e-96,
Organism=Escherichia coli, GI1786764, Length=321, Percent_Identity=54.2056074766355, Blast_Score=345, Evalue=2e-96,
Organism=Escherichia coli, GI1786453, Length=321, Percent_Identity=54.2056074766355, Blast_Score=345, Evalue=2e-96,
Organism=Escherichia coli, GI1788303, Length=321, Percent_Identity=53.8940809968847, Blast_Score=344, Evalue=5e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002559
- InterPro:   IPR008490 [H]

Pfam domain/function: PF05598 DUF772; PF01609 Transposase_11 [H]

EC number: NA

Molecular weight: Translated: 37083; Mature: 37083

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRQIGFAEAEIAGKKRVTRRQRFLEEMEKVVPWQRLLSAIEPHYPKGTRGRPPIGLERM
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCHHHH
LRIYFVQQWYGLSDEGLEDALYDSITLRAFAGIDLAIENVPDATTLLKFRRLLIEHELTR
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHH
KLFDEIGISLCERGLMMKEGTLVDATIIEAPPSTKNAGKSRDPEMHQTKKGNEWHFGMKA
HHHHHHHHHHHHCCCEECCCCEEEEEEEECCCCCCCCCCCCCCCHHHCCCCCCEECCEEE
HIGVDADSGLIHSVVGTAANVSDVSQAHALLHGHEEQVFADAGYIGVDKREEMAGKAVKW
EECCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHCCCCCCCCCHHHHHCCHHEE
HVAARRGKIKAMQEGALKDLVIALERTKAQIRSRVEHPFHIVKNLFQHRKTRYKGLAKNT
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
AQLFSLFALANLVIARNLLRSVHGSSPSCV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKRQIGFAEAEIAGKKRVTRRQRFLEEMEKVVPWQRLLSAIEPHYPKGTRGRPPIGLERM
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCHHHH
LRIYFVQQWYGLSDEGLEDALYDSITLRAFAGIDLAIENVPDATTLLKFRRLLIEHELTR
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHH
KLFDEIGISLCERGLMMKEGTLVDATIIEAPPSTKNAGKSRDPEMHQTKKGNEWHFGMKA
HHHHHHHHHHHHCCCEECCCCEEEEEEEECCCCCCCCCCCCCCCHHHCCCCCCEECCEEE
HIGVDADSGLIHSVVGTAANVSDVSQAHALLHGHEEQVFADAGYIGVDKREEMAGKAVKW
EECCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHCCCCCCCCCHHHHHCCHHEE
HVAARRGKIKAMQEGALKDLVIALERTKAQIRSRVEHPFHIVKNLFQHRKTRYKGLAKNT
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
AQLFSLFALANLVIARNLLRSVHGSSPSCV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9097039; 9097040; 9278503 [H]