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The map label for this gene is rfbD [H]

Identifier: 161525768

GI number: 161525768

Start: 2820754

End: 2821656

Strand: Direct

Name: rfbD [H]

Synonym: Bmul_2599

Alternate gene names: 161525768

Gene position: 2820754-2821656 (Clockwise)

Preceding gene: 161525767

Following gene: 161525769

Centisome position: 81.8

GC content: 64.12

Gene sequence:

>903_bases
GTGCAGACCTCCGATCGCAAAACGATTCTGCTGACCGGCATCAACGGCCAGGTCGGGTTCGAATTGCAGCGTACGCTGCA
GGGGCTCGGCAACGTGGTTGCCCTCGATCGCAGCCGGCTCGACCTGTCGCGCCTCAACCAGGTGCGCGACGTCGTACGCG
ACCTGAAGCCCGATCTGATCGTGAATCCCGCCGCTTATACGGCGGTCGATCAGGCGGAGACCGACGTGGCAATGGCCACA
CGGCTCAATGCGCAAGCGCCGGCCGTGCTCGCCGAAGAAGCGAAGCGCATCGGCGCCGCGCTGATCCATTACTCGACCGA
CTACGTGTTTGCGGGCACGAAAGCAGGCCCGTACGTCGAGGACGATGCGGTCGACCCGCAAAACGTGTACGGCAGGACGA
AGCTCGACGGCGAACGCGCGATCGCGGCATCGGGATGCGCGCATCTGATTTTCCGTACGAGCTGGGTCTACGGTACGCGC
GGCAAGAACTTTCTGCTGACGATGCTGCGGCTCGGCGCCGAGCGCGACGAACTGAAAGTCGTCGCCGACCAGATCGGCGC
GCCGACGTGGTCGAACACGATCGCGACGCTGACTGCGCACGTGGTTGCACAGGGCATCGCCGCGGGCGCGGCTGGCCGGG
AAGCATGGTGGAACGCGCATTCGGGCGTCTATCACCTTTGCGCTGCGGGCTCGACTTCGTGGCATGGATTTGCCGAGGCG
ATTTTCCGTCTTTCGGATATGCAGAAGAAGCCTTCGGTGAAGCCGATTCCCGCCACTGCTTACCCTACGCCCGCGAGCCG
CCCGGCAAATTCGCGCATGTCGAACGAGAAACTGGCCGAGCACTTCGGCTTGCGGGCGCCGCACTGGGTGGCGGCACTGG
AACTCTGCATGAACGGGATGTAA

Upstream 100 bases:

>100_bases
TCGGCATCGAATGGCCGATCGAGTTCGAGCCGCTATTGGCGGCGAAGGATGCGGCAGGCAAACGGCTCGCCGAAGCCGAG
TGCTTCCAGTGAGGGCCGCC

Downstream 100 bases:

>100_bases
CGCAAGCAGCAGAACCAAGCTGCCTGCCGCCATTCAAAAGAGCGGCGGCGGCTTTATTTCCTTCGTTATGAGACTCCCCG
TCTGATGATTGTTTAAAACA

Product: dTDP-4-dehydrorhamnose reductase

Products: NA

Alternate protein names: dTDP-4-keto-L-rhamnose reductase; dTDP-6-deoxy-L-lyxo-4-hexulose reductase; dTDP-6-deoxy-L-mannose dehydrogenase; dTDP-L-rhamnose synthase [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MQTSDRKTILLTGINGQVGFELQRTLQGLGNVVALDRSRLDLSRLNQVRDVVRDLKPDLIVNPAAYTAVDQAETDVAMAT
RLNAQAPAVLAEEAKRIGAALIHYSTDYVFAGTKAGPYVEDDAVDPQNVYGRTKLDGERAIAASGCAHLIFRTSWVYGTR
GKNFLLTMLRLGAERDELKVVADQIGAPTWSNTIATLTAHVVAQGIAAGAAGREAWWNAHSGVYHLCAAGSTSWHGFAEA
IFRLSDMQKKPSVKPIPATAYPTPASRPANSRMSNEKLAEHFGLRAPHWVAALELCMNGM

Sequences:

>Translated_300_residues
MQTSDRKTILLTGINGQVGFELQRTLQGLGNVVALDRSRLDLSRLNQVRDVVRDLKPDLIVNPAAYTAVDQAETDVAMAT
RLNAQAPAVLAEEAKRIGAALIHYSTDYVFAGTKAGPYVEDDAVDPQNVYGRTKLDGERAIAASGCAHLIFRTSWVYGTR
GKNFLLTMLRLGAERDELKVVADQIGAPTWSNTIATLTAHVVAQGIAAGAAGREAWWNAHSGVYHLCAAGSTSWHGFAEA
IFRLSDMQKKPSVKPIPATAYPTPASRPANSRMSNEKLAEHFGLRAPHWVAALELCMNGM
>Mature_300_residues
MQTSDRKTILLTGINGQVGFELQRTLQGLGNVVALDRSRLDLSRLNQVRDVVRDLKPDLIVNPAAYTAVDQAETDVAMAT
RLNAQAPAVLAEEAKRIGAALIHYSTDYVFAGTKAGPYVEDDAVDPQNVYGRTKLDGERAIAASGCAHLIFRTSWVYGTR
GKNFLLTMLRLGAERDELKVVADQIGAPTWSNTIATLTAHVVAQGIAAGAAGREAWWNAHSGVYHLCAAGSTSWHGFAEA
IFRLSDMQKKPSVKPIPATAYPTPASRPANSRMSNEKLAEHFGLRAPHWVAALELCMNGM

Specific function: Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4- hexulose to yield dTDP-L-rhamnose. RmlD uses NADH and NADPH nearly equally well [H]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI11034825, Length=298, Percent_Identity=28.5234899328859, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI33519455, Length=298, Percent_Identity=28.5234899328859, Blast_Score=93, Evalue=3e-19,
Organism=Escherichia coli, GI1788352, Length=300, Percent_Identity=44, Blast_Score=249, Evalue=2e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: =1.1.1.133 [H]

Molecular weight: Translated: 32437; Mature: 32437

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTSDRKTILLTGINGQVGFELQRTLQGLGNVVALDRSRLDLSRLNQVRDVVRDLKPDLI
CCCCCCCEEEEECCCCCCCHHHHHHHHHCCCEEEECHHHCCHHHHHHHHHHHHHCCCCEE
VNPAAYTAVDQAETDVAMATRLNAQAPAVLAEEAKRIGAALIHYSTDYVFAGTKAGPYVE
ECCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEECCCEEEECCCCCCCCC
DDAVDPQNVYGRTKLDGERAIAASGCAHLIFRTSWVYGTRGKNFLLTMLRLGAERDELKV
CCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHCCCHHHHHH
VADQIGAPTWSNTIATLTAHVVAQGIAAGAAGREAWWNAHSGVYHLCAAGSTSWHGFAEA
HHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCEEEEEECCCCCHHHHHHH
IFRLSDMQKKPSVKPIPATAYPTPASRPANSRMSNEKLAEHFGLRAPHWVAALELCMNGM
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MQTSDRKTILLTGINGQVGFELQRTLQGLGNVVALDRSRLDLSRLNQVRDVVRDLKPDLI
CCCCCCCEEEEECCCCCCCHHHHHHHHHCCCEEEECHHHCCHHHHHHHHHHHHHCCCCEE
VNPAAYTAVDQAETDVAMATRLNAQAPAVLAEEAKRIGAALIHYSTDYVFAGTKAGPYVE
ECCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEECCCEEEECCCCCCCCC
DDAVDPQNVYGRTKLDGERAIAASGCAHLIFRTSWVYGTRGKNFLLTMLRLGAERDELKV
CCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHCCCHHHHHH
VADQIGAPTWSNTIATLTAHVVAQGIAAGAAGREAWWNAHSGVYHLCAAGSTSWHGFAEA
HHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCEEEEEECCCCCHHHHHHH
IFRLSDMQKKPSVKPIPATAYPTPASRPANSRMSNEKLAEHFGLRAPHWVAALELCMNGM
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609 [H]