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The map label for this gene is rfbB [H]

Identifier: 161525765

GI number: 161525765

Start: 2818243

End: 2819304

Strand: Direct

Name: rfbB [H]

Synonym: Bmul_2596

Alternate gene names: 161525765

Gene position: 2818243-2819304 (Clockwise)

Preceding gene: 161525764

Following gene: 161525766

Centisome position: 81.72

GC content: 63.28

Gene sequence:

>1062_bases
ATGATATTGGTGACAGGAGGCGCAGGCTTTATCGGCGCCAACTTTGTACTCGACTGGCTGCGCGCATCCGGCGAAGCCGT
GCTCAACGTCGACAAACTGACCTACGCGGGCAACCTTCGTACGCTGCAGTCGCTGGAAGGCAATCCGAAGCACGTGTTTG
CGCGCGTCGATATCTGCGACCGCGCTGCGCTCGACGCGCTCTTTGCCGAGCACAAGCCGCGTGCGGTGCTGCACTTCGCG
GCGGAAAGCCACGTCGATCGCTCGATTCACGGCCCGGCCGACTTTGTGCAGACCAATGTGGTCGGCACCTTCACGCTGCT
GGAAGCTGCCCGCCAATACTGGAACGGCCTGAACGACGCCGACAAGGCCGCCTTCCGCTTCTTGCACGTCTCGACGGACG
AAGTATTCGGCTCGCTGTCCGCCACGGACCCGCAATTCTCGGAGACGACGCCGTACGCGCCGAACAGTCCGTACTCGGCA
ACCAAGGCGGGCTCCGATCATCTCGTTCGCGCATACCATCACACCTACGGCCTGCCGACGCTGACGACGAACTGCTCGAA
CAACTACGGCCCGTACCAGTTCCCGGAAAAACTCATTCCGCTGATGATCGCGAACGCGCTCGCCGGCAAACCGCTGCCCG
TGTATGGCGACGGCCAGAACGTGCGCGACTGGCTGTATGTAGGCGACCACTGCAGCGCGATCCGCGAGGTACTCGCGCGC
GGCGTGCCGGGCGAAACGTACAACGTCGGCGGCTGGAACGAGAAGAAGAACCTCGAGGTCGTGCATACGCTGTGCGACCT
GCTCGACGCGAAGCGGCCGAAGGCAACGGGCTCGTATCGCGATCAGATCACGTACGTGACGGATCGCCCGGGCCACGATC
GCCGCTATGCGATCGATGCGCGCAAGCTCGAGCGCGAACTCGGCTGGAAGCCGGCGGAGACGTTCGAAACCGGTCTCGCG
AAGACGGTCGACTGGTATCTCGACAACCAGCAGTGGGTCGACGAAGTCGCATCGGGCGAGTACCGCAAGTGGGTCGAAAC
CAACTACGCGCAGCGCGCGTAA

Upstream 100 bases:

>100_bases
CGGCCGCGCGGTTTCTTGATATTCTTCGCGCGGCCTTGAACGGGCCTTGCCGACCATGGAGCGCGCAAGGCTCGCGTCCG
CTCAACGTAAAAGGGACTAC

Downstream 100 bases:

>100_bases
GGGTGGCAGAGATGGCACGCAAAGGCATCATTCTCGCGGGCGGTTCCGGCACGCGGCTCTATCCGATCACACAGGCCGTA
TCGAAGCAACTGCTGCCCGT

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MILVTGGAGFIGANFVLDWLRASGEAVLNVDKLTYAGNLRTLQSLEGNPKHVFARVDICDRAALDALFAEHKPRAVLHFA
AESHVDRSIHGPADFVQTNVVGTFTLLEAARQYWNGLNDADKAAFRFLHVSTDEVFGSLSATDPQFSETTPYAPNSPYSA
TKAGSDHLVRAYHHTYGLPTLTTNCSNNYGPYQFPEKLIPLMIANALAGKPLPVYGDGQNVRDWLYVGDHCSAIREVLAR
GVPGETYNVGGWNEKKNLEVVHTLCDLLDAKRPKATGSYRDQITYVTDRPGHDRRYAIDARKLERELGWKPAETFETGLA
KTVDWYLDNQQWVDEVASGEYRKWVETNYAQRA

Sequences:

>Translated_353_residues
MILVTGGAGFIGANFVLDWLRASGEAVLNVDKLTYAGNLRTLQSLEGNPKHVFARVDICDRAALDALFAEHKPRAVLHFA
AESHVDRSIHGPADFVQTNVVGTFTLLEAARQYWNGLNDADKAAFRFLHVSTDEVFGSLSATDPQFSETTPYAPNSPYSA
TKAGSDHLVRAYHHTYGLPTLTTNCSNNYGPYQFPEKLIPLMIANALAGKPLPVYGDGQNVRDWLYVGDHCSAIREVLAR
GVPGETYNVGGWNEKKNLEVVHTLCDLLDAKRPKATGSYRDQITYVTDRPGHDRRYAIDARKLERELGWKPAETFETGLA
KTVDWYLDNQQWVDEVASGEYRKWVETNYAQRA
>Mature_353_residues
MILVTGGAGFIGANFVLDWLRASGEAVLNVDKLTYAGNLRTLQSLEGNPKHVFARVDICDRAALDALFAEHKPRAVLHFA
AESHVDRSIHGPADFVQTNVVGTFTLLEAARQYWNGLNDADKAAFRFLHVSTDEVFGSLSATDPQFSETTPYAPNSPYSA
TKAGSDHLVRAYHHTYGLPTLTTNCSNNYGPYQFPEKLIPLMIANALAGKPLPVYGDGQNVRDWLYVGDHCSAIREVLAR
GVPGETYNVGGWNEKKNLEVVHTLCDLLDAKRPKATGSYRDQITYVTDRPGHDRRYAIDARKLERELGWKPAETFETGLA
KTVDWYLDNQQWVDEVASGEYRKWVETNYAQRA

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=329, Percent_Identity=34.9544072948328, Blast_Score=219, Evalue=4e-57,
Organism=Homo sapiens, GI42516563, Length=354, Percent_Identity=24.8587570621469, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI56237023, Length=348, Percent_Identity=27.8735632183908, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI56118217, Length=348, Percent_Identity=27.8735632183908, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI189083684, Length=348, Percent_Identity=27.8735632183908, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI4504031, Length=343, Percent_Identity=25.6559766763848, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1788353, Length=359, Percent_Identity=61.5598885793872, Blast_Score=464, Evalue=1e-132,
Organism=Escherichia coli, GI48994969, Length=344, Percent_Identity=63.0813953488372, Blast_Score=456, Evalue=1e-129,
Organism=Escherichia coli, GI1786974, Length=353, Percent_Identity=26.628895184136, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788366, Length=352, Percent_Identity=26.4204545454545, Blast_Score=75, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17568069, Length=332, Percent_Identity=35.8433734939759, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI115532424, Length=339, Percent_Identity=31.858407079646, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI71982035, Length=371, Percent_Identity=28.3018867924528, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71982038, Length=373, Percent_Identity=28.1501340482574, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17539532, Length=331, Percent_Identity=24.1691842900302, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17507723, Length=336, Percent_Identity=24.1071428571429, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI133901790, Length=335, Percent_Identity=24.1791044776119, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI133901788, Length=335, Percent_Identity=24.1791044776119, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI133901786, Length=335, Percent_Identity=24.1791044776119, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17539422, Length=335, Percent_Identity=24.1791044776119, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17539424, Length=335, Percent_Identity=24.1791044776119, Blast_Score=71, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6319493, Length=364, Percent_Identity=27.1978021978022, Blast_Score=96, Evalue=9e-21,
Organism=Drosophila melanogaster, GI19923002, Length=364, Percent_Identity=30.2197802197802, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI21356223, Length=331, Percent_Identity=26.2839879154079, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24158427, Length=335, Percent_Identity=26.2686567164179, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 39323; Mature: 39323

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILVTGGAGFIGANFVLDWLRASGEAVLNVDKLTYAGNLRTLQSLEGNPKHVFARVDICD
CEEEECCCCHHHHHHHHHHHHCCCCEEEEEEHEEECCCCHHHHHCCCCCCEEEEEECHHH
RAALDALFAEHKPRAVLHFAAESHVDRSIHGPADFVQTNVVGTFTLLEAARQYWNGLNDA
HHHHHHHHHCCCCCEEEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHH
DKAAFRFLHVSTDEVFGSLSATDPQFSETTPYAPNSPYSATKAGSDHLVRAYHHTYGLPT
HHHHHHEEEEEHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCC
LTTNCSNNYGPYQFPEKLIPLMIANALAGKPLPVYGDGQNVRDWLYVGDHCSAIREVLAR
EEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCHHEEECHHHHHHHHHHHC
GVPGETYNVGGWNEKKNLEVVHTLCDLLDAKRPKATGSYRDQITYVTDRPGHDRRYAIDA
CCCCCEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCEEEHHH
RKLERELGWKPAETFETGLAKTVDWYLDNQQWVDEVASGEYRKWVETNYAQRA
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MILVTGGAGFIGANFVLDWLRASGEAVLNVDKLTYAGNLRTLQSLEGNPKHVFARVDICD
CEEEECCCCHHHHHHHHHHHHCCCCEEEEEEHEEECCCCHHHHHCCCCCCEEEEEECHHH
RAALDALFAEHKPRAVLHFAAESHVDRSIHGPADFVQTNVVGTFTLLEAARQYWNGLNDA
HHHHHHHHHCCCCCEEEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHH
DKAAFRFLHVSTDEVFGSLSATDPQFSETTPYAPNSPYSATKAGSDHLVRAYHHTYGLPT
HHHHHHEEEEEHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCC
LTTNCSNNYGPYQFPEKLIPLMIANALAGKPLPVYGDGQNVRDWLYVGDHCSAIREVLAR
EEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCHHEEECHHHHHHHHHHHC
GVPGETYNVGGWNEKKNLEVVHTLCDLLDAKRPKATGSYRDQITYVTDRPGHDRRYAIDA
CCCCCEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCEEEHHH
RKLERELGWKPAETFETGLAKTVDWYLDNQQWVDEVASGEYRKWVETNYAQRA
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253667 [H]