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The map label for this gene is tolB [H]

Identifier: 161525756

GI number: 161525756

Start: 2810641

End: 2811936

Strand: Direct

Name: tolB [H]

Synonym: Bmul_2587

Alternate gene names: 161525756

Gene position: 2810641-2811936 (Clockwise)

Preceding gene: 161525755

Following gene: 161525757

Centisome position: 81.5

GC content: 64.97

Gene sequence:

>1296_bases
ATGAGTTTGATGACGAAGCTAGGTTTCAGGGCACTCGTGGCCTCGTGCCTGATTGCGGCCGGCAGTGCCGCTAACGCGCA
GGTCAACGTGCTGATCACCGGCGTCGGTTCGACCCAGTTCCCCATCGCCACCGCGAACTTCGCGAATGAGGCAGGCCTGC
CGCAGCAGGTCACGTCGATCGTCCGTGCCGATCTTGCCCGCAGCGGCAAATTCACCAACATCGACGCCGGCAGCACGCCC
GTGCCCGAGACGGCCTCAGTCGATCTCGGCGCGTGGAAGGCCAAGGGCGCGAATGCGTTCGTCGCGGGCAGCGTGAACCA
CGAGGGCAACGGTCAGTACAAGATCAACTTCATCCTGTACGACACCGTGAAGCAGCAAAGCCTCGGCGGCCTGTCGCTCA
GCGCCAACGACACAACGCTGCGCACGGCCGGGCACAAGATCGCCGACTACATCTACCAGAAGCTGCTCGGCGTGCGCGGC
GTGTTCGCGACGCGTCTGTCGTACGTGATCAAGACCGGCAACCGCTACCAGCTGCAGATTTCGGACTCGGACGGCCAGAA
CGCACGCATCGCGCTGTCGAGCACCGAGCCGATCATCTCGCCGGCATGGTCGCCGAGCGGCACGAAGGTCGCGTACGTGT
CGTTCGAGCGCAAGAAGCCGATCGTCTACATCCACGATCTGCCGACCGGCCGCCGCTACATGGTCTCCGATCAGAAGGGC
AACAACAGCGCGCCGGCGTGGTCGCCGGACGGCAATACGCTCGCGGTCGCGTTGTCGCTGACGGGCAACACGCAGATCTA
CTCGGTCAATTCGAACGGCGGCGGCCTGCGCCGTCTCACGCAGAGCAGCTCGATCGACACCGAGCCGTTCTACTCGCCGG
ACGGCCGCTGGATCTACTTCACGAGCGATCGCGGCGGTGCGCCGCAAATCTATCGGATGCCCGCGCAAGGCGAAAGCGCC
GGCGCGGCGCAGCGCGTGACCTTCACCGGCAGCTACAACACGAGTCCGCGCGTGAGCCCGGACGGCAAGCTGCTCGCTTA
CATCTCCCGTACGGGCGGGGGCTTCAAGCTGTACGTTCAGGATCTGCAAACCGGCGCGGCGAACGCTATCACCAACACGA
ATCGCGACGAATCGCCGAGCTTCGCGGCAAACGGCCAGTACATTTTGTACGCCACGCAGTCGGGCGGTCGCAACGTGCTC
GCTGCGGTGCCTTCCGACGGCAGTGCGCCGCCGCAGATTCTGTCCGTCCAGGGCGGCTCGGTTCGTGAGCCGTCGTGGGG
GCCCTTCATGCAATGA

Upstream 100 bases:

>100_bases
CGCTTACACTCCGGCCGTCGCTGTCCGACGGGAACGAATCGGGTATTTTTACAGTCTCTGCGGTTGCGCAGCGATCCATG
TCATACGGGAAGCAAGAAGC

Downstream 100 bases:

>100_bases
CCACAAGGAGAGTAACCATGATGTCGAAAAAAGTTCGTCTGGCCCTGGCCGTGATGATGATCGGCGCGCTCGCAGCGTGT
AAGTCGGGTGTGAAGCTCGA

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MSLMTKLGFRALVASCLIAAGSAANAQVNVLITGVGSTQFPIATANFANEAGLPQQVTSIVRADLARSGKFTNIDAGSTP
VPETASVDLGAWKAKGANAFVAGSVNHEGNGQYKINFILYDTVKQQSLGGLSLSANDTTLRTAGHKIADYIYQKLLGVRG
VFATRLSYVIKTGNRYQLQISDSDGQNARIALSSTEPIISPAWSPSGTKVAYVSFERKKPIVYIHDLPTGRRYMVSDQKG
NNSAPAWSPDGNTLAVALSLTGNTQIYSVNSNGGGLRRLTQSSSIDTEPFYSPDGRWIYFTSDRGGAPQIYRMPAQGESA
GAAQRVTFTGSYNTSPRVSPDGKLLAYISRTGGGFKLYVQDLQTGAANAITNTNRDESPSFAANGQYILYATQSGGRNVL
AAVPSDGSAPPQILSVQGGSVREPSWGPFMQ

Sequences:

>Translated_431_residues
MSLMTKLGFRALVASCLIAAGSAANAQVNVLITGVGSTQFPIATANFANEAGLPQQVTSIVRADLARSGKFTNIDAGSTP
VPETASVDLGAWKAKGANAFVAGSVNHEGNGQYKINFILYDTVKQQSLGGLSLSANDTTLRTAGHKIADYIYQKLLGVRG
VFATRLSYVIKTGNRYQLQISDSDGQNARIALSSTEPIISPAWSPSGTKVAYVSFERKKPIVYIHDLPTGRRYMVSDQKG
NNSAPAWSPDGNTLAVALSLTGNTQIYSVNSNGGGLRRLTQSSSIDTEPFYSPDGRWIYFTSDRGGAPQIYRMPAQGESA
GAAQRVTFTGSYNTSPRVSPDGKLLAYISRTGGGFKLYVQDLQTGAANAITNTNRDESPSFAANGQYILYATQSGGRNVL
AAVPSDGSAPPQILSVQGGSVREPSWGPFMQ
>Mature_430_residues
SLMTKLGFRALVASCLIAAGSAANAQVNVLITGVGSTQFPIATANFANEAGLPQQVTSIVRADLARSGKFTNIDAGSTPV
PETASVDLGAWKAKGANAFVAGSVNHEGNGQYKINFILYDTVKQQSLGGLSLSANDTTLRTAGHKIADYIYQKLLGVRGV
FATRLSYVIKTGNRYQLQISDSDGQNARIALSSTEPIISPAWSPSGTKVAYVSFERKKPIVYIHDLPTGRRYMVSDQKGN
NSAPAWSPDGNTLAVALSLTGNTQIYSVNSNGGGLRRLTQSSSIDTEPFYSPDGRWIYFTSDRGGAPQIYRMPAQGESAG
AAQRVTFTGSYNTSPRVSPDGKLLAYISRTGGGFKLYVQDLQTGAANAITNTNRDESPSFAANGQYILYATQSGGRNVLA
AVPSDGSAPPQILSVQGGSVREPSWGPFMQ

Specific function: Involved in the tonB-independent uptake of proteins [H]

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family [H]

Homologues:

Organism=Escherichia coli, GI1786961, Length=439, Percent_Identity=37.8132118451025, Blast_Score=261, Evalue=6e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943 [H]

Pfam domain/function: PF07676 PD40; PF04052 TolB_N [H]

EC number: NA

Molecular weight: Translated: 45618; Mature: 45487

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLMTKLGFRALVASCLIAAGSAANAQVNVLITGVGSTQFPIATANFANEAGLPQQVTSI
CCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCEEECCCCCCCCCHHHHHHH
VRADLARSGKFTNIDAGSTPVPETASVDLGAWKAKGANAFVAGSVNHEGNGQYKINFILY
HHHHHHHCCCEEECCCCCCCCCCCCEECCCCEECCCCCEEEEECCCCCCCCEEEEEEEEE
DTVKQQSLGGLSLSANDTTLRTAGHKIADYIYQKLLGVRGVFATRLSYVIKTGNRYQLQI
ECCCCCCCCCEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEE
SDSDGQNARIALSSTEPIISPAWSPSGTKVAYVSFERKKPIVYIHDLPTGRRYMVSDQKG
ECCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEECCCCCEEEEECCCC
NNSAPAWSPDGNTLAVALSLTGNTQIYSVNSNGGGLRRLTQSSSIDTEPFYSPDGRWIYF
CCCCCCCCCCCCEEEEEEEECCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCEEEE
TSDRGGAPQIYRMPAQGESAGAAQRVTFTGSYNTSPRVSPDGKLLAYISRTGGGFKLYVQ
ECCCCCCCEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCCEEEEEE
DLQTGAANAITNTNRDESPSFAANGQYILYATQSGGRNVLAAVPSDGSAPPQILSVQGGS
ECCCCCCHHCCCCCCCCCCCEECCCCEEEEEECCCCCEEEEECCCCCCCCCEEEEECCCC
VREPSWGPFMQ
CCCCCCCCCCC
>Mature Secondary Structure 
SLMTKLGFRALVASCLIAAGSAANAQVNVLITGVGSTQFPIATANFANEAGLPQQVTSI
CHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCEEECCCCCCCCCHHHHHHH
VRADLARSGKFTNIDAGSTPVPETASVDLGAWKAKGANAFVAGSVNHEGNGQYKINFILY
HHHHHHHCCCEEECCCCCCCCCCCCEECCCCEECCCCCEEEEECCCCCCCCEEEEEEEEE
DTVKQQSLGGLSLSANDTTLRTAGHKIADYIYQKLLGVRGVFATRLSYVIKTGNRYQLQI
ECCCCCCCCCEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEE
SDSDGQNARIALSSTEPIISPAWSPSGTKVAYVSFERKKPIVYIHDLPTGRRYMVSDQKG
ECCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEECCCCCEEEEECCCC
NNSAPAWSPDGNTLAVALSLTGNTQIYSVNSNGGGLRRLTQSSSIDTEPFYSPDGRWIYF
CCCCCCCCCCCCEEEEEEEECCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCEEEE
TSDRGGAPQIYRMPAQGESAGAAQRVTFTGSYNTSPRVSPDGKLLAYISRTGGGFKLYVQ
ECCCCCCCEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCCEEEEEE
DLQTGAANAITNTNRDESPSFAANGQYILYATQSGGRNVLAAVPSDGSAPPQILSVQGGS
ECCCCCCHHCCCCCCCCCCCEECCCCEEEEEECCCCCEEEEECCCCCCCCCEEEEECCCC
VREPSWGPFMQ
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA