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The map label for this gene is mhpA [H]

Identifier: 161525735

GI number: 161525735

Start: 2793698

End: 2795365

Strand: Reverse

Name: mhpA [H]

Synonym: Bmul_2566

Alternate gene names: 161525735

Gene position: 2795365-2793698 (Counterclockwise)

Preceding gene: 161525737

Following gene: 161525734

Centisome position: 81.06

GC content: 70.08

Gene sequence:

>1668_bases
ATGAACGTCGATTACAAGAGCCTGAAGTTTGCGTACCGCACGCGCGCGGAGCGCGCGATTGCCGACGATGCGGCCGTCCG
GCCCGTGATCGTCGTCGGCGCGGGGCCGGTCGGTCTGGCCGCGGCAATCGATCTCGCGCAGCAGGGCGTGCCCGTCGTGC
TGCTCGACGACGACGACACGCTGTCCACCGGTTCGCGTGCGATCTGCTTCGCGAAGCGCACGCTCGAGATCTTCGACCGG
CTCGGCTGCGGCGAACGCTTCGTCGACAAGGGCGTGAGCTGGCACGTCGGCAAGGTGTTCCTGCAGGACGAGCAGCTGTA
CGCGTTCGACCTGCTGCCGGAACAAGGGCATGCGCGTCCCGCCTTCATCAATCTGCAGCAGTACTACGTCGAAGGGTATC
TGGCCGAGCGCGCGTTCGAGTTGCCGAACCTCGAGATCCGCTGGAAGCACAAGGTGACCGGCATCGAGCAGGCGGCCGAG
CATGCGGTGCTGACGGTCGAGACGCCCGAAGGCGTCGACACGCTGCGCGCGCAGTACGTGATCGCGGCCGACGGCTCGCG
CAGCCCGATGCGCACGATGATGGGCCTCGAAAGCCGCGGCCGCACGTTCAAGGATCGCTTCCTGATCGCGGACGTGAAGA
TGAAGGCCGACTTCCCGACCGAACGCTGGTTCTGGTTCGATCCGCCGTTTCATCGCAACCAGTCGGTGCTGCTGCACCGT
CAGCCGGACAACGTCTGGCGGATCGACTTCCAGCTCGGCTGGGACGCCGATCCGGTCGCCGAGAAGCAGCCGGAGCGCGT
GATTCCGCGCGTGCGCGCGCTGCTCGGGCCGGACGTCGAGTTCGAGCTCGAATGGGTCAGCATCTATACGTTCCGCTGCC
AGCGGATGGACACGTTCCGGCACGGCCGCGTGCTGTTCGCGGGCGATTCCGCGCACGGCGTGTCGCCGTTCGGCGCGCGC
GGCGCAAACAGCGGCGTGCAGGACGCGGACAACCTCGCGTGGAAGCTGAAGCTCGTGCTGGACGGCCGCGCGCCGGACCG
GCTGCTCGACACGTATGCGAGCGAGCGCGAATACGCGGCCGACGAAAACATCCGCCACTCGACGCGCTCGACCGACTTCA
TCACGCCGAAAAGCGCGGTGTCGCGCGTGTTCCGCGATGCGACGCTGAAGCTCGCGCGCGACTGCGAGTTCGCACGCAAG
ATCGTGAACAGCGGGCGGCTGTCGACGCCCGCGGTGCTCGACGGTTCGCCGCTGAACACGCCCGATCGCGTGGGCGAGAC
GTTCGCGTGTGCGATGCGTCCGGGCGCGGCCGCGGCCGACGCGCCGGTGCGCGCCGAGCACGGCGCGAGCTGGCTGCTGC
CGCATCTGCACGGCGGCTTCGCCGGCGTGCTGTTCGGCCTGCCGGGCGACGGCGCAGCGCTCGCGCAGGCGGTCGCGGAT
CTCGCGCTGCCGGTGCGGCCGGTGGTCGTCGTGCCGGCCGGCCACGCGCAGCCGGTGGCCGGCGTCGACGTCGTCGAGGA
CGTCGACGGGCTCGCCGCGCAGCGCTACGACGCGCGGCCGGGCACCTTCTATCTGCTGCGTCCCGACCAGCACGTCTGCG
CGCGGATGCGCGCGCTCGACCGCCAGGCGATCGCCGAGGCGCTGGCCCGCGCGATCTGCGCCGGCTGA

Upstream 100 bases:

>100_bases
AATGGACGGAGGACGCGGGCGGGCCGGCAGGCCGGTACGCGACGGGCGCAACGACGGCGTGCGCGGGTGCGCGCGTCGCG
GCAGTGGGGGCGCGCCGGTC

Downstream 100 bases:

>100_bases
ATACGACAACGATACGGAGACACCGTCATGCCCCCACTCGATACCCGCGCGCGTCTCGCCGATCCGGACGCGTTCTACGA
AGCGCTGATCGACATGCATC

Product: FAD-dependent oxidoreductase

Products: H2O; 3-(2,3-dihydroxyphenyl)propionate; NAD [C]

Alternate protein names: 3-HCI hydroxylase; 3-HPP hydroxylase [H]

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MNVDYKSLKFAYRTRAERAIADDAAVRPVIVVGAGPVGLAAAIDLAQQGVPVVLLDDDDTLSTGSRAICFAKRTLEIFDR
LGCGERFVDKGVSWHVGKVFLQDEQLYAFDLLPEQGHARPAFINLQQYYVEGYLAERAFELPNLEIRWKHKVTGIEQAAE
HAVLTVETPEGVDTLRAQYVIAADGSRSPMRTMMGLESRGRTFKDRFLIADVKMKADFPTERWFWFDPPFHRNQSVLLHR
QPDNVWRIDFQLGWDADPVAEKQPERVIPRVRALLGPDVEFELEWVSIYTFRCQRMDTFRHGRVLFAGDSAHGVSPFGAR
GANSGVQDADNLAWKLKLVLDGRAPDRLLDTYASEREYAADENIRHSTRSTDFITPKSAVSRVFRDATLKLARDCEFARK
IVNSGRLSTPAVLDGSPLNTPDRVGETFACAMRPGAAAADAPVRAEHGASWLLPHLHGGFAGVLFGLPGDGAALAQAVAD
LALPVRPVVVVPAGHAQPVAGVDVVEDVDGLAAQRYDARPGTFYLLRPDQHVCARMRALDRQAIAEALARAICAG

Sequences:

>Translated_555_residues
MNVDYKSLKFAYRTRAERAIADDAAVRPVIVVGAGPVGLAAAIDLAQQGVPVVLLDDDDTLSTGSRAICFAKRTLEIFDR
LGCGERFVDKGVSWHVGKVFLQDEQLYAFDLLPEQGHARPAFINLQQYYVEGYLAERAFELPNLEIRWKHKVTGIEQAAE
HAVLTVETPEGVDTLRAQYVIAADGSRSPMRTMMGLESRGRTFKDRFLIADVKMKADFPTERWFWFDPPFHRNQSVLLHR
QPDNVWRIDFQLGWDADPVAEKQPERVIPRVRALLGPDVEFELEWVSIYTFRCQRMDTFRHGRVLFAGDSAHGVSPFGAR
GANSGVQDADNLAWKLKLVLDGRAPDRLLDTYASEREYAADENIRHSTRSTDFITPKSAVSRVFRDATLKLARDCEFARK
IVNSGRLSTPAVLDGSPLNTPDRVGETFACAMRPGAAAADAPVRAEHGASWLLPHLHGGFAGVLFGLPGDGAALAQAVAD
LALPVRPVVVVPAGHAQPVAGVDVVEDVDGLAAQRYDARPGTFYLLRPDQHVCARMRALDRQAIAEALARAICAG
>Mature_555_residues
MNVDYKSLKFAYRTRAERAIADDAAVRPVIVVGAGPVGLAAAIDLAQQGVPVVLLDDDDTLSTGSRAICFAKRTLEIFDR
LGCGERFVDKGVSWHVGKVFLQDEQLYAFDLLPEQGHARPAFINLQQYYVEGYLAERAFELPNLEIRWKHKVTGIEQAAE
HAVLTVETPEGVDTLRAQYVIAADGSRSPMRTMMGLESRGRTFKDRFLIADVKMKADFPTERWFWFDPPFHRNQSVLLHR
QPDNVWRIDFQLGWDADPVAEKQPERVIPRVRALLGPDVEFELEWVSIYTFRCQRMDTFRHGRVLFAGDSAHGVSPFGAR
GANSGVQDADNLAWKLKLVLDGRAPDRLLDTYASEREYAADENIRHSTRSTDFITPKSAVSRVFRDATLKLARDCEFARK
IVNSGRLSTPAVLDGSPLNTPDRVGETFACAMRPGAAAADAPVRAEHGASWLLPHLHGGFAGVLFGLPGDGAALAQAVAD
LALPVRPVVVVPAGHAQPVAGVDVVEDVDGLAAQRYDARPGTFYLLRPDQHVCARMRALDRQAIAEALARAICAG

Specific function: Catalyzes the insertion of one atom of molecular oxygen into position 2 of the phenyl ring of 3-(3- hydroxyphenyl)propionate (3-HPP) and hydroxycinnamic acid (3HCI) [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=520, Percent_Identity=27.5, Blast_Score=132, Evalue=6e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 61200; Mature: 61200

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVDYKSLKFAYRTRAERAIADDAAVRPVIVVGAGPVGLAAAIDLAQQGVPVVLLDDDDT
CCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHCCCCEEEECCCCC
LSTGSRAICFAKRTLEIFDRLGCGERFVDKGVSWHVGKVFLQDEQLYAFDLLPEQGHARP
CCCCCEEEEEHHHHHHHHHHCCCCHHHHHCCCCEEEEEEEEECCEEEEEECCCCCCCCCC
AFINLQQYYVEGYLAERAFELPNLEIRWKHKVTGIEQAAEHAVLTVETPEGVDTLRAQYV
EEEEHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHCCEEEEEECCCCCHHHHEEEE
IAADGSRSPMRTMMGLESRGRTFKDRFLIADVKMKADFPTERWFWFDPPFHRNQSVLLHR
EEECCCCCHHHHHHHHHHCCCEECCEEEEEEEEEECCCCCCCEEEECCCCCCCCEEEEEE
QPDNVWRIDFQLGWDADPVAEKQPERVIPRVRALLGPDVEFELEWVSIYTFRCQRMDTFR
CCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEEEEEHHHHHHHC
HGRVLFAGDSAHGVSPFGARGANSGVQDADNLAWKLKLVLDGRAPDRLLDTYASEREYAA
CCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHCCCCCCC
DENIRHSTRSTDFITPKSAVSRVFRDATLKLARDCEFARKIVNSGRLSTPAVLDGSPLNT
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCC
PDRVGETFACAMRPGAAAADAPVRAEHGASWLLPHLHGGFAGVLFGLPGDGAALAQAVAD
HHHHCCEEEEEECCCCCCCCCCCCCCCCCCEECHHHCCCCEEEEEECCCCHHHHHHHHHH
LALPVRPVVVVPAGHAQPVAGVDVVEDVDGLAAQRYDARPGTFYLLRPDQHVCARMRALD
HHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHH
RQAIAEALARAICAG
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MNVDYKSLKFAYRTRAERAIADDAAVRPVIVVGAGPVGLAAAIDLAQQGVPVVLLDDDDT
CCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHCCCCEEEECCCCC
LSTGSRAICFAKRTLEIFDRLGCGERFVDKGVSWHVGKVFLQDEQLYAFDLLPEQGHARP
CCCCCEEEEEHHHHHHHHHHCCCCHHHHHCCCCEEEEEEEEECCEEEEEECCCCCCCCCC
AFINLQQYYVEGYLAERAFELPNLEIRWKHKVTGIEQAAEHAVLTVETPEGVDTLRAQYV
EEEEHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHCCEEEEEECCCCCHHHHEEEE
IAADGSRSPMRTMMGLESRGRTFKDRFLIADVKMKADFPTERWFWFDPPFHRNQSVLLHR
EEECCCCCHHHHHHHHHHCCCEECCEEEEEEEEEECCCCCCCEEEECCCCCCCCEEEEEE
QPDNVWRIDFQLGWDADPVAEKQPERVIPRVRALLGPDVEFELEWVSIYTFRCQRMDTFR
CCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEEEEEHHHHHHHC
HGRVLFAGDSAHGVSPFGARGANSGVQDADNLAWKLKLVLDGRAPDRLLDTYASEREYAA
CCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHCCCCCCC
DENIRHSTRSTDFITPKSAVSRVFRDATLKLARDCEFARKIVNSGRLSTPAVLDGSPLNT
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCC
PDRVGETFACAMRPGAAAADAPVRAEHGASWLLPHLHGGFAGVLFGLPGDGAALAQAVAD
HHHHCCEEEEEECCCCCCCCCCCCCCCCCCEECHHHCCCCEEEEEECCCCHHHHHHHHHH
LALPVRPVVVVPAGHAQPVAGVDVVEDVDGLAAQRYDARPGTFYLLRPDQHVCARMRALD
HHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHH
RQAIAEALARAICAG
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-(3-hydroxyphenyl)propionate; NADH; O2 [C]

Specific reaction: 3-(3-hydroxyphenyl)propionate + NADH + O2 = H2O + 3-(2,3-dihydroxyphenyl)propionate + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA