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The map label for this gene is pur8 [H]

Identifier: 161525711

GI number: 161525711

Start: 2763952

End: 2765391

Strand: Direct

Name: pur8 [H]

Synonym: Bmul_2542

Alternate gene names: 161525711

Gene position: 2763952-2765391 (Clockwise)

Preceding gene: 161525710

Following gene: 161525712

Centisome position: 80.15

GC content: 71.11

Gene sequence:

>1440_bases
ATGACACACGGCATCCACGGCGAAAAGCGCTGGTACGCACTGATCGTCCTCTGCCTCGGCGTGCTGATGATCGTGCTCGA
CAGCACGATCGTGAACGTCGCGCTGCCGTCGATCGGCACCGATCTGCACTTCACCGAGACGGCGCTCGTCTGGGTCGTCA
ACGCGTATCTGCTGACGTTCGGCGGCTGCCTGCTGCTCGGCGGCCGGCTCGGCGATCTGTACGGACAGCGGCGCATGTTC
CTCGCCGGCCTCGTCGTGTTCACGCTCGCGTCGCTCGCGTGCGGACTCGCGCCCTCGCAGACGATGCTGATCGCCGCGCG
CGCCGTGCAGGGGCTCGGCGGCGCGGTGGTATCGGCCGTGTCGCTGTCGCTGATCATGAATCTGTTTACCGAGCCCGGCG
AACGGGCGCGCGCGATGGGCGTGTACGGCTTCGTGTGCGCGGGCGGCGGCAGCATCGGCGTGCTGCTCGGCGGCCTGCTG
ACGAGCGCGCTGTCCTGGCACTGGATCTTCCTCGTGAACCTGCCGATCGGCATCGCCGTCTACGCGCTGTGCGCGGCACT
GTTGCCGCGTACGCGCGTGCCGGCCGGCGCCGGGCAGCTCGACGTCGCCGGTGCGATCACGGTCACCGCGTCGCTGATGC
TCGCGGTCTACGGAATCGTCGGCGGCAACGAGGCCGGCTGGCTGTCGGCGCAGACCGTTGCACTGATCGGCGCGGCCGTC
GTGCTGCTTGCGCTGTTCGTCGCGATCGAGTTGCACGTCGCGCATCCGCTGATGCCGCTCACGCTGTTCGCCGCACGCAA
CGTCGCGCTCGCGAACATCATCAGCGTGCTGTGGGCGGCCGCGATGTTCGCGTGGTTCTTCCTGTCCGCGCTGTACCTGC
AGCGCGTGCTCGGCTACGGGCCGCTGCAGGTCGGCCTCGCGTTCCTGCCCGCGAACCTGATCATGGCCGCGTTCTCGCTC
GGGCTGTCCGCGCGGATCGTCATGCGTTTCGGGATTCGCGGCCCGATCGCGGCCGGCCTGCTGATCGCCGCGTGCGGGCT
CGCGCTGTTCGCGCGCGCGCCCGTCGACGGCGGCTTCGTCTGGCACGTGCTGCCCGGCATGACGCTGCTCGGTATCGGCG
CCGGCATCGCGTTCAACCCGGTGCTGCTCGCCGCCATGAGCGACGTCGATCCGGCCGATTCGGGGCTCGCGTCGGGCATC
GTCAACACCGCGTTCATGATGGGCGGCGCGCTCGGCCTCGCGGTGCTCGCGAGCGTCGCGGCGGCGCGGACCGATGCGCT
CGCGGCCGCGCAGGTCGCGCCGCTCGGCGCGCTGAACGGCGGCTACCACGCGGCATTCGCATTCGGCGCGGCGTTTGCGG
CGCTCGCCGCCGCGATCGGCATCGCGCTGCGCGTGCGTCCGGCGCCGGCCGACAGCGGCGTCGGCGCTTCGCTGCACTGA

Upstream 100 bases:

>100_bases
CGGCCGCTACGCGATGCTGCTGCCACAAACCGGCAGCAGACGCGTACGAATGCCGTCTACAATCCAGCCTTTCAAATCCC
TATCGGAAGGTTGCGAGCCC

Downstream 100 bases:

>100_bases
CGCGCGGCGGGCGGCCCTGGGCGCCGTCGGGCCGCCCGCCGCAGCCGGTACGGCTGCCCGCAGGCGCCACACGCCGCCGC
GTGCAGCCACCGTTCAAACG

Product: EmrB/QacA family drug resistance transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MTHGIHGEKRWYALIVLCLGVLMIVLDSTIVNVALPSIGTDLHFTETALVWVVNAYLLTFGGCLLLGGRLGDLYGQRRMF
LAGLVVFTLASLACGLAPSQTMLIAARAVQGLGGAVVSAVSLSLIMNLFTEPGERARAMGVYGFVCAGGGSIGVLLGGLL
TSALSWHWIFLVNLPIGIAVYALCAALLPRTRVPAGAGQLDVAGAITVTASLMLAVYGIVGGNEAGWLSAQTVALIGAAV
VLLALFVAIELHVAHPLMPLTLFAARNVALANIISVLWAAAMFAWFFLSALYLQRVLGYGPLQVGLAFLPANLIMAAFSL
GLSARIVMRFGIRGPIAAGLLIAACGLALFARAPVDGGFVWHVLPGMTLLGIGAGIAFNPVLLAAMSDVDPADSGLASGI
VNTAFMMGGALGLAVLASVAAARTDALAAAQVAPLGALNGGYHAAFAFGAAFAALAAAIGIALRVRPAPADSGVGASLH

Sequences:

>Translated_479_residues
MTHGIHGEKRWYALIVLCLGVLMIVLDSTIVNVALPSIGTDLHFTETALVWVVNAYLLTFGGCLLLGGRLGDLYGQRRMF
LAGLVVFTLASLACGLAPSQTMLIAARAVQGLGGAVVSAVSLSLIMNLFTEPGERARAMGVYGFVCAGGGSIGVLLGGLL
TSALSWHWIFLVNLPIGIAVYALCAALLPRTRVPAGAGQLDVAGAITVTASLMLAVYGIVGGNEAGWLSAQTVALIGAAV
VLLALFVAIELHVAHPLMPLTLFAARNVALANIISVLWAAAMFAWFFLSALYLQRVLGYGPLQVGLAFLPANLIMAAFSL
GLSARIVMRFGIRGPIAAGLLIAACGLALFARAPVDGGFVWHVLPGMTLLGIGAGIAFNPVLLAAMSDVDPADSGLASGI
VNTAFMMGGALGLAVLASVAAARTDALAAAQVAPLGALNGGYHAAFAFGAAFAALAAAIGIALRVRPAPADSGVGASLH
>Mature_478_residues
THGIHGEKRWYALIVLCLGVLMIVLDSTIVNVALPSIGTDLHFTETALVWVVNAYLLTFGGCLLLGGRLGDLYGQRRMFL
AGLVVFTLASLACGLAPSQTMLIAARAVQGLGGAVVSAVSLSLIMNLFTEPGERARAMGVYGFVCAGGGSIGVLLGGLLT
SALSWHWIFLVNLPIGIAVYALCAALLPRTRVPAGAGQLDVAGAITVTASLMLAVYGIVGGNEAGWLSAQTVALIGAAVV
LLALFVAIELHVAHPLMPLTLFAARNVALANIISVLWAAAMFAWFFLSALYLQRVLGYGPLQVGLAFLPANLIMAAFSLG
LSARIVMRFGIRGPIAAGLLIAACGLALFARAPVDGGFVWHVLPGMTLLGIGAGIAFNPVLLAAMSDVDPADSGLASGIV
NTAFMMGGALGLAVLASVAAARTDALAAAQVAPLGALNGGYHAAFAFGAAFAALAAAIGIALRVRPAPADSGVGASLH

Specific function: May be involved in active puromycin efflux energized by a proton-dependent electrochemical gradient. In addition, it could be implicated in secreting N-acetylpuromycin, the last intermediate of the puromycin biosynthesis pathway, to the environment [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI87081983, Length=426, Percent_Identity=33.5680751173709, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1789042, Length=450, Percent_Identity=26.2222222222222, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1790195, Length=419, Percent_Identity=30.0715990453461, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1788710, Length=309, Percent_Identity=30.42071197411, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1788392, Length=366, Percent_Identity=30.0546448087432, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1790146, Length=169, Percent_Identity=27.810650887574, Blast_Score=66, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6324954, Length=396, Percent_Identity=25.7575757575758, Blast_Score=110, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6323520, Length=468, Percent_Identity=25.2136752136752, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6325455, Length=260, Percent_Identity=26.9230769230769, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6319770, Length=396, Percent_Identity=25.2525252525253, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6322958, Length=382, Percent_Identity=24.0837696335079, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6321663, Length=154, Percent_Identity=31.8181818181818, Blast_Score=70, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6323735, Length=313, Percent_Identity=25.2396166134185, Blast_Score=64, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 48700; Mature: 48568

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHGIHGEKRWYALIVLCLGVLMIVLDSTIVNVALPSIGTDLHFTETALVWVVNAYLLTF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
GGCLLLGGRLGDLYGQRRMFLAGLVVFTLASLACGLAPSQTMLIAARAVQGLGGAVVSAV
CHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHH
SLSLIMNLFTEPGERARAMGVYGFVCAGGGSIGVLLGGLLTSALSWHWIFLVNLPIGIAV
HHHHHHHHHCCCCHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHEEEEEECCHHHHH
YALCAALLPRTRVPAGAGQLDVAGAITVTASLMLAVYGIVGGNEAGWLSAQTVALIGAAV
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
VLLALFVAIELHVAHPLMPLTLFAARNVALANIISVLWAAAMFAWFFLSALYLQRVLGYG
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PLQVGLAFLPANLIMAAFSLGLSARIVMRFGIRGPIAAGLLIAACGLALFARAPVDGGFV
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCH
WHVLPGMTLLGIGAGIAFNPVLLAAMSDVDPADSGLASGIVNTAFMMGGALGLAVLASVA
HHHHCCHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AARTDALAAAQVAPLGALNGGYHAAFAFGAAFAALAAAIGIALRVRPAPADSGVGASLH
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
THGIHGEKRWYALIVLCLGVLMIVLDSTIVNVALPSIGTDLHFTETALVWVVNAYLLTF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
GGCLLLGGRLGDLYGQRRMFLAGLVVFTLASLACGLAPSQTMLIAARAVQGLGGAVVSAV
CHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHH
SLSLIMNLFTEPGERARAMGVYGFVCAGGGSIGVLLGGLLTSALSWHWIFLVNLPIGIAV
HHHHHHHHHCCCCHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHEEEEEECCHHHHH
YALCAALLPRTRVPAGAGQLDVAGAITVTASLMLAVYGIVGGNEAGWLSAQTVALIGAAV
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
VLLALFVAIELHVAHPLMPLTLFAARNVALANIISVLWAAAMFAWFFLSALYLQRVLGYG
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PLQVGLAFLPANLIMAAFSLGLSARIVMRFGIRGPIAAGLLIAACGLALFARAPVDGGFV
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCH
WHVLPGMTLLGIGAGIAFNPVLLAAMSDVDPADSGLASGIVNTAFMMGGALGLAVLASVA
HHHHCCHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AARTDALAAAQVAPLGALNGGYHAAFAFGAAFAALAAAIGIALRVRPAPADSGVGASLH
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7916693 [H]