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The map label for this gene is argT [H]

Identifier: 161525707

GI number: 161525707

Start: 2759987

End: 2760766

Strand: Reverse

Name: argT [H]

Synonym: Bmul_2538

Alternate gene names: 161525707

Gene position: 2760766-2759987 (Counterclockwise)

Preceding gene: 161525713

Following gene: 161525706

Centisome position: 80.06

GC content: 66.28

Gene sequence:

>780_bases
ATGAACTGGAAGCTTTCCCTTTGCGCCGCCGCGGCGCTCGCCTGCGCGGCCGTCACCGTCCACGCGGAACCGACCACGTT
GCGCTTCGGGATCGAAGCCGCCTATCCGCCGTTCGAGAGCAAGACGCCGGCCGGCCAGTTGCAGGGCTTCGACGTCGACA
TCGGCAACGCCGTGTGCGCGAAGCTGAACATGAAGTGCGTGTGGGTCGAGAACGCGTTCGACGGCCTGATTCCCGCGCTG
CAGGCGCGCAAGTTCGACGTGATCAATTCGGCGATGAACATCACCGCGAAGCGCAAGCAGAGCATCGATTTCACGCCGCC
GATCTACGTGGTGCCGATCGTGATGATCGCGAAGCGCGGCTCGCCGTTGCGGCCCGAGGTCGCGAGCGTGCGCGGTAAGC
GCGTCGGCGTGCTGCAGGGCTCGTCGCAGGAGGATTTCCTGAAGGCGCACTGGGCCAATGCGGGCGTGTCGGTCGTGTCG
TATCAGGATCAGGACCAGATCTATGCGGATCTCGTCGCCGGGCGGCTGGACGCCGCCGTGCAGGAAGCGCAGACCGCGCA
GGAGGGCTTCCTCGACAAGCCGGCCGGCCGCGACTACCAGATCGTCGGCGAGCCGCTCAAGGATCCGGCGACGCTCGGCG
AAGGCACGGGCTTCGGGCTGCGCAAGGGCGACAAGGCGCTGCAGGCGAAGGTCGTCGGCGCGCTCGATGCGCTGAAGAAG
GACGGCACGTTGAGCGCGCTGTCGCAGAAGTACTTCAAGCGCGACATCATCGCGAAGTAA

Upstream 100 bases:

>100_bases
TGCCCACAGACATGGGGCCGCCGCACCGGCCGCCGCTTCGTCGCCCAGCGCGATCGCGTCGCGCGTCCGATTTTCATCGC
TGAACCCTGAAGGACCCGTC

Downstream 100 bases:

>100_bases
GCGCGAGCGTGCGCCGGCCGCGGTGCCGGCGCGCGCTGCATCGACCTTCTACGCTGGACGGGCAAGCATGGATTTCGACG
TGATCGTTCTGGGGGCCGGC

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: LAO-binding protein [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MNWKLSLCAAAALACAAVTVHAEPTTLRFGIEAAYPPFESKTPAGQLQGFDVDIGNAVCAKLNMKCVWVENAFDGLIPAL
QARKFDVINSAMNITAKRKQSIDFTPPIYVVPIVMIAKRGSPLRPEVASVRGKRVGVLQGSSQEDFLKAHWANAGVSVVS
YQDQDQIYADLVAGRLDAAVQEAQTAQEGFLDKPAGRDYQIVGEPLKDPATLGEGTGFGLRKGDKALQAKVVGALDALKK
DGTLSALSQKYFKRDIIAK

Sequences:

>Translated_259_residues
MNWKLSLCAAAALACAAVTVHAEPTTLRFGIEAAYPPFESKTPAGQLQGFDVDIGNAVCAKLNMKCVWVENAFDGLIPAL
QARKFDVINSAMNITAKRKQSIDFTPPIYVVPIVMIAKRGSPLRPEVASVRGKRVGVLQGSSQEDFLKAHWANAGVSVVS
YQDQDQIYADLVAGRLDAAVQEAQTAQEGFLDKPAGRDYQIVGEPLKDPATLGEGTGFGLRKGDKALQAKVVGALDALKK
DGTLSALSQKYFKRDIIAK
>Mature_259_residues
MNWKLSLCAAAALACAAVTVHAEPTTLRFGIEAAYPPFESKTPAGQLQGFDVDIGNAVCAKLNMKCVWVENAFDGLIPAL
QARKFDVINSAMNITAKRKQSIDFTPPIYVVPIVMIAKRGSPLRPEVASVRGKRVGVLQGSSQEDFLKAHWANAGVSVVS
YQDQDQIYADLVAGRLDAAVQEAQTAQEGFLDKPAGRDYQIVGEPLKDPATLGEGTGFGLRKGDKALQAKVVGALDALKK
DGTLSALSQKYFKRDIIAK

Specific function: This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=252, Percent_Identity=45.6349206349206, Blast_Score=244, Evalue=6e-66,
Organism=Escherichia coli, GI1788648, Length=233, Percent_Identity=46.3519313304721, Blast_Score=235, Evalue=3e-63,
Organism=Escherichia coli, GI1787085, Length=226, Percent_Identity=33.6283185840708, Blast_Score=148, Evalue=5e-37,
Organism=Escherichia coli, GI1787088, Length=229, Percent_Identity=32.7510917030568, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1788228, Length=235, Percent_Identity=31.4893617021277, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1787031, Length=249, Percent_Identity=29.3172690763052, Blast_Score=96, Evalue=2e-21,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27777; Mature: 27777

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNWKLSLCAAAALACAAVTVHAEPTTLRFGIEAAYPPFESKTPAGQLQGFDVDIGNAVCA
CCCCHHHHHHHHHHHHHEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCEEECCCHHHH
KLNMKCVWVENAFDGLIPALQARKFDVINSAMNITAKRKQSIDFTPPIYVVPIVMIAKRG
CCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCC
SPLRPEVASVRGKRVGVLQGSSQEDFLKAHWANAGVSVVSYQDQDQIYADLVAGRLDAAV
CCCCCHHHHCCCCEEEEEECCCHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHH
QEAQTAQEGFLDKPAGRDYQIVGEPLKDPATLGEGTGFGLRKGDKALQAKVVGALDALKK
HHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
DGTLSALSQKYFKRDIIAK
CCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNWKLSLCAAAALACAAVTVHAEPTTLRFGIEAAYPPFESKTPAGQLQGFDVDIGNAVCA
CCCCHHHHHHHHHHHHHEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCEEECCCHHHH
KLNMKCVWVENAFDGLIPALQARKFDVINSAMNITAKRKQSIDFTPPIYVVPIVMIAKRG
CCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCC
SPLRPEVASVRGKRVGVLQGSSQEDFLKAHWANAGVSVVSYQDQDQIYADLVAGRLDAAV
CCCCCHHHHCCCCEEEEEECCCHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHH
QEAQTAQEGFLDKPAGRDYQIVGEPLKDPATLGEGTGFGLRKGDKALQAKVVGALDALKK
HHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
DGTLSALSQKYFKRDIIAK
CCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3040734; 9298646 [H]