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The map label for this gene is pyrC' [H]

Identifier: 161525689

GI number: 161525689

Start: 2739588

End: 2740874

Strand: Reverse

Name: pyrC' [H]

Synonym: Bmul_2520

Alternate gene names: 161525689

Gene position: 2740874-2739588 (Counterclockwise)

Preceding gene: 161525690

Following gene: 161525688

Centisome position: 79.48

GC content: 71.1

Gene sequence:

>1287_bases
ATGAAGATTCATATCAAAGGCGGCACGCTGATCGATCCGGTCGCCGGCAGCGAGCGGCAAGCCGACGTATTCATCTCGGG
CGGCAAGATCGACGCGATCGCCGAAGCCGGCCGCGCGCCGGCCGGCTTCGCCGCCGACAAGACGATCGACGCGACGGGCC
AGATCGTCGCGCCCGGCCTCGTCGACCTGTGCGCGCGGCTGCGCGAGCCCGGCTACGAGCACAAGGCGACGCTCGCGTCC
GAGATGGCGGCGGCCGTCGCCGGCGGCGTGACGACGCTCGTGTGTCCGCCCGACACCGATCCGGTGCTCGACGAGCCGGG
CCTCGTCGAGATGCTGAAGTTCCGCGCGCGCAATCTGCACCAGGCCAACGTGCATCCGCTCGGCGCGCTGACCGTCGGGC
TGAAGGGCGAAGTGATCACCGAGATGGTCGCGCTCACCGAGTCCGGCTGCGTCGGCTTCACGCATGCGAACGTGCCGCTG
CGCGACACGCAGGTGTTGCTGCGCGCACTGCAGTACGCGAGCACCTACGGCTACACCACCTGGCTGCGCCCGCTCGACGC
GTTCATCGGCAAGGGCGGCGTCGCGGCGAGCGGGCCCGTCGCGTCGCGGCTCGGGCTGTCGGGCGTGCCGGTCGCGGCCG
AGACGATCGCGCTGCATACGATTTTCGAACTGATGCGCGTGACCGGCGCGCGCGTGCACCTCGCGCGGCTGTCGTCGGCG
GCGGGCCTCGCGCTCGTGCGCGCGGCGAAGGCCGAAGGGCTGCCCGTCACGTGCGACGTCGGCGTGAATCACCTGCACCT
GATCGACGTCGACATCGGCTATTTCGATTCGCAGTTCCGGCTCGATCCGCCGCTGCGCAGCGAGCGCGATCGCGAGGCCA
TCCGCGCGGCGCTCGCGGACGGCACCATCGACGCGATCTGCTCCGATCACACGCCGGTCGACGACGACGAGAAGCTGCTG
CCGTTCGGCGAAGCGACGCCCGGCGCGACCGGCCTCGAACTGCTGCTGTCGCTGACCGTGAAGTGGGCCGACGAGACGCG
CACGCCGCTCGCGCAGGCGCTGCGCCGCATCACGTCGGCGCCTGCGGACGTGCTGAATCTGCCGGCCGGCCGTCTGACCG
AAGGCAGTGCGGCCGATCTCTGCGTGTTCGACCCGCGCGCGCACTGGCGCGTCGAGCCGCGCGCGCTGAAGAGCCAAGGC
CACAACTCGCCGTTCCTCGGCTACGAGCTGCCCGCATGCGTGCGTGCGACGCTCGTCGCCGGGCAGGTCGCGTTCGAGCG
CAACTGA

Upstream 100 bases:

>100_bases
GGATGGCCGTGATGGGCATCGTCGCCGGCAACAACGACTGAGCCGCTTCGCGCCTCCTTATCCAGGCATTCAACGCATTC
AACGCATTCACAGACAGCGC

Downstream 100 bases:

>100_bases
ACCACGCCGGGCTTTCGTCATGAACGCTATCCGCAAGCTGCGCCTCGTCTTTCACCTGTTGCGCGGCATGGCGATCGTCG
CGCTGCGCTTTTCGCACGTG

Product: dihydroorotase

Products: Allantoate; H+ [C]

Alternate protein names: Aspartate carbamoyltransferase 44 kDa Non-catalytic chain [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MKIHIKGGTLIDPVAGSERQADVFISGGKIDAIAEAGRAPAGFAADKTIDATGQIVAPGLVDLCARLREPGYEHKATLAS
EMAAAVAGGVTTLVCPPDTDPVLDEPGLVEMLKFRARNLHQANVHPLGALTVGLKGEVITEMVALTESGCVGFTHANVPL
RDTQVLLRALQYASTYGYTTWLRPLDAFIGKGGVAASGPVASRLGLSGVPVAAETIALHTIFELMRVTGARVHLARLSSA
AGLALVRAAKAEGLPVTCDVGVNHLHLIDVDIGYFDSQFRLDPPLRSERDREAIRAALADGTIDAICSDHTPVDDDEKLL
PFGEATPGATGLELLLSLTVKWADETRTPLAQALRRITSAPADVLNLPAGRLTEGSAADLCVFDPRAHWRVEPRALKSQG
HNSPFLGYELPACVRATLVAGQVAFERN

Sequences:

>Translated_428_residues
MKIHIKGGTLIDPVAGSERQADVFISGGKIDAIAEAGRAPAGFAADKTIDATGQIVAPGLVDLCARLREPGYEHKATLAS
EMAAAVAGGVTTLVCPPDTDPVLDEPGLVEMLKFRARNLHQANVHPLGALTVGLKGEVITEMVALTESGCVGFTHANVPL
RDTQVLLRALQYASTYGYTTWLRPLDAFIGKGGVAASGPVASRLGLSGVPVAAETIALHTIFELMRVTGARVHLARLSSA
AGLALVRAAKAEGLPVTCDVGVNHLHLIDVDIGYFDSQFRLDPPLRSERDREAIRAALADGTIDAICSDHTPVDDDEKLL
PFGEATPGATGLELLLSLTVKWADETRTPLAQALRRITSAPADVLNLPAGRLTEGSAADLCVFDPRAHWRVEPRALKSQG
HNSPFLGYELPACVRATLVAGQVAFERN
>Mature_428_residues
MKIHIKGGTLIDPVAGSERQADVFISGGKIDAIAEAGRAPAGFAADKTIDATGQIVAPGLVDLCARLREPGYEHKATLAS
EMAAAVAGGVTTLVCPPDTDPVLDEPGLVEMLKFRARNLHQANVHPLGALTVGLKGEVITEMVALTESGCVGFTHANVPL
RDTQVLLRALQYASTYGYTTWLRPLDAFIGKGGVAASGPVASRLGLSGVPVAAETIALHTIFELMRVTGARVHLARLSSA
AGLALVRAAKAEGLPVTCDVGVNHLHLIDVDIGYFDSQFRLDPPLRSERDREAIRAALADGTIDAICSDHTPVDDDEKLL
PFGEATPGATGLELLLSLTVKWADETRTPLAQALRRITSAPADVLNLPAGRLTEGSAADLCVFDPRAHWRVEPRALKSQG
HNSPFLGYELPACVRATLVAGQVAFERN

Specific function: Non-functional DHOase [H]

COG id: COG0044

COG function: function code F; Dihydroorotase and related cyclic amidohydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. PyrC' subfamily [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=398, Percent_Identity=27.3869346733668, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI1786722, Length=452, Percent_Identity=27.212389380531, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI87082175, Length=444, Percent_Identity=25.6756756756757, Blast_Score=95, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI193204318, Length=403, Percent_Identity=25.3101736972705, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI86575075, Length=429, Percent_Identity=24.2424242424242, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322218, Length=401, Percent_Identity=24.9376558603491, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24642586, Length=372, Percent_Identity=27.1505376344086, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI18859883, Length=210, Percent_Identity=30.4761904761905, Blast_Score=86, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR004722
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: 3.5.2.5 [C]

Molecular weight: Translated: 45149; Mature: 45149

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIHIKGGTLIDPVAGSERQADVFISGGKIDAIAEAGRAPAGFAADKTIDATGQIVAPGL
CEEEECCCCEECCCCCCCCCCEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCCEECCCH
VDLCARLREPGYEHKATLASEMAAAVAGGVTTLVCPPDTDPVLDEPGLVEMLKFRARNLH
HHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHCCCC
QANVHPLGALTVGLKGEVITEMVALTESGCVGFTHANVPLRDTQVLLRALQYASTYGYTT
CCCCCCCEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCHHH
WLRPLDAFIGKGGVAASGPVASRLGLSGVPVAAETIALHTIFELMRVTGARVHLARLSSA
HHHHHHHHHCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEHHHCCC
AGLALVRAAKAEGLPVTCDVGVNHLHLIDVDIGYFDSQFRLDPPLRSERDREAIRAALAD
CCHHHHHHHCCCCCCEEEECCCCEEEEEEEECCCCCCCEECCCCCCCHHHHHHHHHHHHC
GTIDAICSDHTPVDDDEKLLPFGEATPGATGLELLLSLTVKWADETRTPLAQALRRITSA
CCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCC
PADVLNLPAGRLTEGSAADLCVFDPRAHWRVEPRALKSQGHNSPFLGYELPACVRATLVA
CHHHHCCCCCCCCCCCCCCEEEECCCCCEEECHHHHHCCCCCCCCCCCCCHHHHHHHHHH
GQVAFERN
HCEEECCC
>Mature Secondary Structure
MKIHIKGGTLIDPVAGSERQADVFISGGKIDAIAEAGRAPAGFAADKTIDATGQIVAPGL
CEEEECCCCEECCCCCCCCCCEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCCEECCCH
VDLCARLREPGYEHKATLASEMAAAVAGGVTTLVCPPDTDPVLDEPGLVEMLKFRARNLH
HHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHCCCC
QANVHPLGALTVGLKGEVITEMVALTESGCVGFTHANVPLRDTQVLLRALQYASTYGYTT
CCCCCCCEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCHHH
WLRPLDAFIGKGGVAASGPVASRLGLSGVPVAAETIALHTIFELMRVTGARVHLARLSSA
HHHHHHHHHCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEHHHCCC
AGLALVRAAKAEGLPVTCDVGVNHLHLIDVDIGYFDSQFRLDPPLRSERDREAIRAALAD
CCHHHHHHHCCCCCCEEEECCCCEEEEEEEECCCCCCCEECCCCCCCHHHHHHHHHHHHC
GTIDAICSDHTPVDDDEKLLPFGEATPGATGLELLLSLTVKWADETRTPLAQALRRITSA
CCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCC
PADVLNLPAGRLTEGSAADLCVFDPRAHWRVEPRALKSQGHNSPFLGYELPACVRATLVA
CHHHHCCCCCCCCCCCCCCEEEECCCCCEEECHHHHHCCCCCCCCCCCCCHHHHHHHHHH
GQVAFERN
HCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Allantoin; H2O [C]

Specific reaction: Allantoin + H2O --> Allantoate + H+ [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: Dithiothreitol; p-Chloromercuribenzene-sulphonic acid [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]