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The map label for this gene is tfdB [H]

Identifier: 161525630

GI number: 161525630

Start: 2673144

End: 2674931

Strand: Reverse

Name: tfdB [H]

Synonym: Bmul_2460

Alternate gene names: 161525630

Gene position: 2674931-2673144 (Counterclockwise)

Preceding gene: 161525631

Following gene: 161525629

Centisome position: 77.57

GC content: 63.65

Gene sequence:

>1788_bases
ATGGAGACACTCAACAGGATGAAAACATCGGTTCTGATCGTCGGTGCCGGACCGGCGGGCCTGACGGCGTCCGCGATACT
GGCAAGAAACGGGATCGATGCGTTGACGATATGTCGATACTCCGGAACGGCGAACTCGCCGCGTGCGCACATCACGAACC
AGCGCACGATGGAAGTGTTCCGGGACATGGGTATCGAAGAGCGCATGGTGGCGCTGTCGACGCCCAATCGCTTGATGGGC
AACACGACGTGGATGACGAGCTTTACCGGCATCGAACTGGCTCGCTTGCCTTCCTGGGGAACGGGCATCGATCGGCGTAC
CGACTATGAACGCGCGAGCCCGAGCGCGATGTGCAACCTCCCGCAGCACATCCTCGAGCCGGCCCTGCTGGATGCCGCCC
GGGCGGCGGGCGCGCGCGTACGGTTCGACACGGTACTCGTCGCGATCCGGCAGACCAAAGATGCCGTCTATGCGACGTTG
CTGGATCGGCTGACCGGCGCGAAATACGAAGTCGAGTCCGAGTATGTGATCGGCGCGGACGGCGCCAACAGTCTCGTCGC
GAGCGAACTCGGCTTCGAGATGGAGGGACACCGGGGCGTCGCCGAGCAGGTGAACTGCTGGGTCGAGGTCGATTTGTCGA
AGTACGTCTCGCATCGGCCCAGTTCGCTCTATGTCATCGCGCAGCCGGGGAACAACTTCTGGATCGGCAGCGGCGTATGG
ATTTGCGTGAAGCCCTGGTCGGAATGGGTGTTGCTGTTCGCGTTCCGCTCGGCCGACGGCACACCGGATCTCAGCGAACA
AGCCGTCGTCGAGTATGCGCGGTCGACGATCGGCGATCCCGATATCGACGTAAAGGTCAAATCGGTCTCGACGTGGGCAA
TCAACCACATGTTCGCGAAGGAGATGCGCAAAGGGCGTGCGTTCATCGCAGGCGACGCAGCGCACCGCCATCCGCCGGCG
AACGGCCTGGGTTCCAACACGTCAATCCAGGACAGTTTCAATCTCGCGTGGAAGCTCGCCATGGTGCTGAAGGGCCAGGC
AAGCGACGCGCTGCTGGACAGCTATTCCGCCGAGCGGCAGCCGGTGGCGCGGCAGGTGGTCGAACGCGCGAACAAATCGG
TCGAGCTGATGCAGCCGATGTCTGCCGCATTCGGATTCAGCCACGGCCAGAGCATCGAGGCCGGCTGGGCGTCGATCGCG
GAACTGCGCGGAGATAGTCCACGCGGCCGGGAACGCCGCAAGCAACTCGCGCGGGCGCTGAAAGGGCTGCAGCACCACTT
CAACGCGCACGGCGTCGAGCTCGGCCAGCATTACACGTCGAGCGCAATCGTGGACGACGGTACGCCCGAATTCACGCCCG
AGCGCGATCCCGAGTTGTATGTCACGCCGACCACGCGTCCGGGTGCACATGTGCCGCACGCGTGGGTGGAGCAGGGCCAG
AAACTGGTCTCGACGCTGGACCTCGTCGGCAAGAACGGCTTCACCGTGGTGACCGGAATCGGCGGACAGCGCTGGTGCGA
AGCAGCGGAACGTCTTGCGGTCGAGTTCGGCATGCCGGTGCGTCATGTGGCCATCGGTCCGGGCCTCGATGCCGAAGACA
TCTACGGTCAGTGGAGTGCCTTGCGGGAAATCGAGGAGAACGGGTGTTTGCTCGTCCGTCCCGACATGCACGTCGCATTC
CGTGCGATGGCGATGTCCGATCGGCCCTTCGAGGATCTGCTCGCCGCCATGGAGCACATCGTCGGGCGGCGGCTGATCAG
TCTGCAACCGTTCGCGAGCCATGCATAA

Upstream 100 bases:

>100_bases
CGACTTCCGAAAAACAGCTACGGCAAGGTGTTGAAGACCGAGTTGCGTAGCGCGGATTGAGCGCGCCGGACTGACGCATG
AAAAGACAGAGGGACAGGAA

Downstream 100 bases:

>100_bases
CCGCGAACCGCGCCGAGTGGCGACGCAAGGTCACAGAAACGTTGAGGAAGACATGAGCAAAATCGTGGTACGAGAGTTGG
CCTATGTGAGACTGAGATCG

Product: monooxygenase FAD-binding

Products: NA

Alternate protein names: 2,4-dichlorophenol hydroxylase; 2,4-DCP hydroxylase [H]

Number of amino acids: Translated: 595; Mature: 595

Protein sequence:

>595_residues
METLNRMKTSVLIVGAGPAGLTASAILARNGIDALTICRYSGTANSPRAHITNQRTMEVFRDMGIEERMVALSTPNRLMG
NTTWMTSFTGIELARLPSWGTGIDRRTDYERASPSAMCNLPQHILEPALLDAARAAGARVRFDTVLVAIRQTKDAVYATL
LDRLTGAKYEVESEYVIGADGANSLVASELGFEMEGHRGVAEQVNCWVEVDLSKYVSHRPSSLYVIAQPGNNFWIGSGVW
ICVKPWSEWVLLFAFRSADGTPDLSEQAVVEYARSTIGDPDIDVKVKSVSTWAINHMFAKEMRKGRAFIAGDAAHRHPPA
NGLGSNTSIQDSFNLAWKLAMVLKGQASDALLDSYSAERQPVARQVVERANKSVELMQPMSAAFGFSHGQSIEAGWASIA
ELRGDSPRGRERRKQLARALKGLQHHFNAHGVELGQHYTSSAIVDDGTPEFTPERDPELYVTPTTRPGAHVPHAWVEQGQ
KLVSTLDLVGKNGFTVVTGIGGQRWCEAAERLAVEFGMPVRHVAIGPGLDAEDIYGQWSALREIEENGCLLVRPDMHVAF
RAMAMSDRPFEDLLAAMEHIVGRRLISLQPFASHA

Sequences:

>Translated_595_residues
METLNRMKTSVLIVGAGPAGLTASAILARNGIDALTICRYSGTANSPRAHITNQRTMEVFRDMGIEERMVALSTPNRLMG
NTTWMTSFTGIELARLPSWGTGIDRRTDYERASPSAMCNLPQHILEPALLDAARAAGARVRFDTVLVAIRQTKDAVYATL
LDRLTGAKYEVESEYVIGADGANSLVASELGFEMEGHRGVAEQVNCWVEVDLSKYVSHRPSSLYVIAQPGNNFWIGSGVW
ICVKPWSEWVLLFAFRSADGTPDLSEQAVVEYARSTIGDPDIDVKVKSVSTWAINHMFAKEMRKGRAFIAGDAAHRHPPA
NGLGSNTSIQDSFNLAWKLAMVLKGQASDALLDSYSAERQPVARQVVERANKSVELMQPMSAAFGFSHGQSIEAGWASIA
ELRGDSPRGRERRKQLARALKGLQHHFNAHGVELGQHYTSSAIVDDGTPEFTPERDPELYVTPTTRPGAHVPHAWVEQGQ
KLVSTLDLVGKNGFTVVTGIGGQRWCEAAERLAVEFGMPVRHVAIGPGLDAEDIYGQWSALREIEENGCLLVRPDMHVAF
RAMAMSDRPFEDLLAAMEHIVGRRLISLQPFASHA
>Mature_595_residues
METLNRMKTSVLIVGAGPAGLTASAILARNGIDALTICRYSGTANSPRAHITNQRTMEVFRDMGIEERMVALSTPNRLMG
NTTWMTSFTGIELARLPSWGTGIDRRTDYERASPSAMCNLPQHILEPALLDAARAAGARVRFDTVLVAIRQTKDAVYATL
LDRLTGAKYEVESEYVIGADGANSLVASELGFEMEGHRGVAEQVNCWVEVDLSKYVSHRPSSLYVIAQPGNNFWIGSGVW
ICVKPWSEWVLLFAFRSADGTPDLSEQAVVEYARSTIGDPDIDVKVKSVSTWAINHMFAKEMRKGRAFIAGDAAHRHPPA
NGLGSNTSIQDSFNLAWKLAMVLKGQASDALLDSYSAERQPVARQVVERANKSVELMQPMSAAFGFSHGQSIEAGWASIA
ELRGDSPRGRERRKQLARALKGLQHHFNAHGVELGQHYTSSAIVDDGTPEFTPERDPELYVTPTTRPGAHVPHAWVEQGQ
KLVSTLDLVGKNGFTVVTGIGGQRWCEAAERLAVEFGMPVRHVAIGPGLDAEDIYGQWSALREIEENGCLLVRPDMHVAF
RAMAMSDRPFEDLLAAMEHIVGRRLISLQPFASHA

Specific function: Transforms 2,4-dichlorophenol (2,4-DCP) into 3,5- dichlorocatechol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=381, Percent_Identity=26.7716535433071, Blast_Score=108, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: =1.14.13.20 [H]

Molecular weight: Translated: 65186; Mature: 65186

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METLNRMKTSVLIVGAGPAGLTASAILARNGIDALTICRYSGTANSPRAHITNQRTMEVF
CCHHHCCCEEEEEEECCCCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHHHHHH
RDMGIEERMVALSTPNRLMGNTTWMTSFTGIELARLPSWGTGIDRRTDYERASPSAMCNL
HHCCHHHEEEEECCCCHHHCCCEEEEEECCEEEEECCCCCCCCCCCCCCCCCCCHHHHCC
PQHILEPALLDAARAAGARVRFDTVLVAIRQTKDAVYATLLDRLTGAKYEVESEYVIGAD
CHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEECC
GANSLVASELGFEMEGHRGVAEQVNCWVEVDLSKYVSHRPSSLYVIAQPGNNFWIGSGVW
CCCHHHHHHCCCCCCCCCCCHHHCCEEEEEEHHHHHCCCCCEEEEEEECCCCEEECCCEE
ICVKPWSEWVLLFAFRSADGTPDLSEQAVVEYARSTIGDPDIDVKVKSVSTWAINHMFAK
EEECCHHHEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHH
EMRKGRAFIAGDAAHRHPPANGLGSNTSIQDSFNLAWKLAMVLKGQASDALLDSYSAERQ
HHHCCCEEEECCCHHCCCCCCCCCCCCCCCCCHHHHHEEEHHHCCCCCHHHHHHHCCCCC
PVARQVVERANKSVELMQPMSAAFGFSHGQSIEAGWASIAELRGDSPRGRERRKQLARAL
HHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHH
KGLQHHFNAHGVELGQHYTSSAIVDDGTPEFTPERDPELYVTPTTRPGAHVPHAWVEQGQ
HHHHHHCCCCCHHHCCHHCCCEEECCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHH
KLVSTLDLVGKNGFTVVTGIGGQRWCEAAERLAVEFGMPVRHVAIGPGLDAEDIYGQWSA
HHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHH
LREIEENGCLLVRPDMHVAFRAMAMSDRPFEDLLAAMEHIVGRRLISLQPFASHA
HHHHHHCCEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEECCCCCCC
>Mature Secondary Structure
METLNRMKTSVLIVGAGPAGLTASAILARNGIDALTICRYSGTANSPRAHITNQRTMEVF
CCHHHCCCEEEEEEECCCCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHHHHHH
RDMGIEERMVALSTPNRLMGNTTWMTSFTGIELARLPSWGTGIDRRTDYERASPSAMCNL
HHCCHHHEEEEECCCCHHHCCCEEEEEECCEEEEECCCCCCCCCCCCCCCCCCCHHHHCC
PQHILEPALLDAARAAGARVRFDTVLVAIRQTKDAVYATLLDRLTGAKYEVESEYVIGAD
CHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEECC
GANSLVASELGFEMEGHRGVAEQVNCWVEVDLSKYVSHRPSSLYVIAQPGNNFWIGSGVW
CCCHHHHHHCCCCCCCCCCCHHHCCEEEEEEHHHHHCCCCCEEEEEEECCCCEEECCCEE
ICVKPWSEWVLLFAFRSADGTPDLSEQAVVEYARSTIGDPDIDVKVKSVSTWAINHMFAK
EEECCHHHEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHH
EMRKGRAFIAGDAAHRHPPANGLGSNTSIQDSFNLAWKLAMVLKGQASDALLDSYSAERQ
HHHCCCEEEECCCHHCCCCCCCCCCCCCCCCCHHHHHEEEHHHCCCCCHHHHHHHCCCCC
PVARQVVERANKSVELMQPMSAAFGFSHGQSIEAGWASIAELRGDSPRGRERRKQLARAL
HHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHH
KGLQHHFNAHGVELGQHYTSSAIVDDGTPEFTPERDPELYVTPTTRPGAHVPHAWVEQGQ
HHHHHHCCCCCHHHCCHHCCCEEECCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHH
KLVSTLDLVGKNGFTVVTGIGGQRWCEAAERLAVEFGMPVRHVAIGPGLDAEDIYGQWSA
HHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHH
LREIEENGCLLVRPDMHVAFRAMAMSDRPFEDLLAAMEHIVGRRLISLQPFASHA
HHHHHHCCEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12949179; 11572451 [H]