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The map label for this gene is spoT [H]

Identifier: 161525569

GI number: 161525569

Start: 2612299

End: 2614665

Strand: Reverse

Name: spoT [H]

Synonym: Bmul_2399

Alternate gene names: 161525569

Gene position: 2614665-2612299 (Counterclockwise)

Preceding gene: 161525570

Following gene: 161525568

Centisome position: 75.82

GC content: 65.1

Gene sequence:

>2367_bases
ATGAGCACCACCCCATCGTCCGCCTCCGCGGATGCGATCACCGAAGACACGGTCCGGTCGCCGGCGCGCCAGTACATCGA
CGCGGTCCTCGAACAGTCCTTCCGGCACCTGTTCGGGCCGACCGCCACTCCGGAGCAGCCGCGCAAGCACGGCGTCGTTT
CGATTGCGAATCTGACGGCCGCGCTTGCCGAGTATCTCGGTCCGGACGAAATCAAAGAGGTGAAGGCGGCGTTCCATTTC
AGCGACGAAGCCCACCTCGGTCAATATCGCCAGAGCGGCGAACCCTATATCACCCATCCTGTCGCCGTCGCCGAAATCTG
TGCGGGCTGGAAGCTCGACGCGCAGGCGATCATGGCGGCGCTGCTGCACGACGTGATGGAAGACCAGGGCGTCACGAAGA
GCGAGCTCGCCGAGCGCTTCGGCCCGAAGGTCGCCGAGCTCGTCGACGGCCTGTCGAAACTCGACAAGATGGAATTCCGC
AGCCGCGAGGAAGCGCAGGCGGAAAACTTCCGCAAGATGCTGCTCGCGATGGCGCGCGACGTGCGCGTGATCCTCGTGAA
GCTCGCCGACCGCCTGCACAACATGCGCACGCTCGGCGCGGTGCCGATGGAAAAGCGTCGCCGCGTCGCGCGCGAAACGC
TCGATATCTATGCGCCGATCGCGCACCGGCTCGGTCTGAACAACACGTATCGCGAGCTGCAGGACATGAGCTTCGCGAAC
TTCAATCCGCATCGCTACGCGACGCTCGAGAAGGCCGTGAAGGCCGCGCGCGGCAATCGCCGCGAGGTGATCGGCAAGAT
CCTCGAGGCCGCGCAGCGCGCGATGGCGGATGCGAAGCTCGACGCCGAGATCACCGGGCGCGAAAAGACGATCTACAGCA
TTTACCGCAAGATGCGCGACAAGCAGCTGTCGTTCTCGCAGGTGCTCGACGTGTACGGCTTTCGCGTCGTCGTCGACAGC
CCGCTCGACTGCTACACGTGCATCGGCGCGCTGCATGCGCTCTACAAGCCCGTGCCGGGCAAGTTCAAGGACTACATCGC
GATCCCGAAGATCAACGGCTATCAGTCGCTGCACACGACGCTCGTCGGGCCGTTCGGCGCGCCGATCGAGTTCCAGGTGC
GCACACGCAAGATGCACGAGATCGCGGAAGCCGGCGTGGCCGCGCACTGGCTCTACAAGAACGGCGGCGCAGATCTGAAC
GATGTGCAGAAGCGCGCGCATCAGTGGCTGAAATCGCTGCTCGACATCCAGAGCGAAGCCGGCGATTCGAGCGAATTCCT
CGAGCACGTGAAGATCGACCTGTTCCCCGACGCGGTCTACGTGTTCACGCCGAAGTCGAAGATCATGGCGCTGCCGCGCG
GCGCGACCGCGCTCGACTTCGCGTATTCGATCCACAGCGATCTGGGCAACCAGTGCGTTGCCGTGAAGATCAACAACGAA
CTGCTGCCGCTGCGCACCGAGCTGAAGAGCGGCGACATCGTCGAGGTGATCACCGCGCCGTATTCGAAGCCGAACCCGGC
GTGGCTCGGCTTCGTGCGCACCGGCAAGGCGCGTTCGGCGATTCGTCATTATCTGAAGACGATGCGGCTGAACGAGTCGG
TGCAGCTGGGCGAGCGGCTCGTCGATCAGAGCCTGAAGGGCTACGGCCTGTCGCTGGCCGACGTCGCGCCCGAGGTGTGG
GAAAAACTCGTGCAGTGGACGGGCAACAAGAGCCGTCAGGAAATCTTCGCCGACATCGGCCTCGGCCGCCGCGTCGCGGC
GGTGATGGCCAAGCGCATCGAGGTGCTGATGAGCGGCCGCGACGCCGACGACGATCTGCCGAAGGGCGAGCGCCACGTCG
TGCAGCACGCGCCGCCGGTCGTCATCACCGGCACGGAAGGGATGTCGGTGCAGTTGTCCGCGTGCTGCCGGCCGATCCCG
GGCGACGCGATCATGGGCTATATCGGCATCGGCCTCGGGATGGCGATCCATACGACCGATTGCCGTGTCGCGCAGCGCAT
CCATCGCCGCGATCCGGGCCGCTGGATCGATGTCGAATGGGCGCCGCAGCCGGGCCGGCTGTTCGACGTCGCGGTGAAGG
CGCTCGTGAAGAATACGAAGGGCATCTTCGCGCGCGTGGCGGCCGACATCACGTCGGCCGACGCGAACATCGTGCACATC
GCGATGGACGAGGATCTCACGCACGAATCGACGGTGCTGCGCTTCGTGATCCAGGTCAGCGATCGCGTGCATCTGGCAAA
CGTGATGCGCCGCGTGCGCACGAATCCGGACGTCATGCGCATCATGCGCGAGCGCTCGACCGACGACGGTACGCATGCGC
GCCACGACGGCGGAATGCGCATCGAGCGCGAGCGGCAGGATTACTGA

Upstream 100 bases:

>100_bases
GTCGGCCTCGAGATGTTGAAGAAGGTGCCGGTGTAACGCGCACCCGGCCCGTTTCAGCCATGTAATCCACCGCGCGCAAC
GACTCGACCAGGAGGCGAAT

Downstream 100 bases:

>100_bases
CCGGGGTTGAATCGAATGACGAAACGGCGGCCGCGTGAACTGCACCCCAAAAGTTGGACTAGATCCAACCTTTGGGGTGT
TTTTCATGACGAAGTACGAC

Product: (p)ppGpp synthetase I SpoT/RelA

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 788; Mature: 787

Protein sequence:

>788_residues
MSTTPSSASADAITEDTVRSPARQYIDAVLEQSFRHLFGPTATPEQPRKHGVVSIANLTAALAEYLGPDEIKEVKAAFHF
SDEAHLGQYRQSGEPYITHPVAVAEICAGWKLDAQAIMAALLHDVMEDQGVTKSELAERFGPKVAELVDGLSKLDKMEFR
SREEAQAENFRKMLLAMARDVRVILVKLADRLHNMRTLGAVPMEKRRRVARETLDIYAPIAHRLGLNNTYRELQDMSFAN
FNPHRYATLEKAVKAARGNRREVIGKILEAAQRAMADAKLDAEITGREKTIYSIYRKMRDKQLSFSQVLDVYGFRVVVDS
PLDCYTCIGALHALYKPVPGKFKDYIAIPKINGYQSLHTTLVGPFGAPIEFQVRTRKMHEIAEAGVAAHWLYKNGGADLN
DVQKRAHQWLKSLLDIQSEAGDSSEFLEHVKIDLFPDAVYVFTPKSKIMALPRGATALDFAYSIHSDLGNQCVAVKINNE
LLPLRTELKSGDIVEVITAPYSKPNPAWLGFVRTGKARSAIRHYLKTMRLNESVQLGERLVDQSLKGYGLSLADVAPEVW
EKLVQWTGNKSRQEIFADIGLGRRVAAVMAKRIEVLMSGRDADDDLPKGERHVVQHAPPVVITGTEGMSVQLSACCRPIP
GDAIMGYIGIGLGMAIHTTDCRVAQRIHRRDPGRWIDVEWAPQPGRLFDVAVKALVKNTKGIFARVAADITSADANIVHI
AMDEDLTHESTVLRFVIQVSDRVHLANVMRRVRTNPDVMRIMRERSTDDGTHARHDGGMRIERERQDY

Sequences:

>Translated_788_residues
MSTTPSSASADAITEDTVRSPARQYIDAVLEQSFRHLFGPTATPEQPRKHGVVSIANLTAALAEYLGPDEIKEVKAAFHF
SDEAHLGQYRQSGEPYITHPVAVAEICAGWKLDAQAIMAALLHDVMEDQGVTKSELAERFGPKVAELVDGLSKLDKMEFR
SREEAQAENFRKMLLAMARDVRVILVKLADRLHNMRTLGAVPMEKRRRVARETLDIYAPIAHRLGLNNTYRELQDMSFAN
FNPHRYATLEKAVKAARGNRREVIGKILEAAQRAMADAKLDAEITGREKTIYSIYRKMRDKQLSFSQVLDVYGFRVVVDS
PLDCYTCIGALHALYKPVPGKFKDYIAIPKINGYQSLHTTLVGPFGAPIEFQVRTRKMHEIAEAGVAAHWLYKNGGADLN
DVQKRAHQWLKSLLDIQSEAGDSSEFLEHVKIDLFPDAVYVFTPKSKIMALPRGATALDFAYSIHSDLGNQCVAVKINNE
LLPLRTELKSGDIVEVITAPYSKPNPAWLGFVRTGKARSAIRHYLKTMRLNESVQLGERLVDQSLKGYGLSLADVAPEVW
EKLVQWTGNKSRQEIFADIGLGRRVAAVMAKRIEVLMSGRDADDDLPKGERHVVQHAPPVVITGTEGMSVQLSACCRPIP
GDAIMGYIGIGLGMAIHTTDCRVAQRIHRRDPGRWIDVEWAPQPGRLFDVAVKALVKNTKGIFARVAADITSADANIVHI
AMDEDLTHESTVLRFVIQVSDRVHLANVMRRVRTNPDVMRIMRERSTDDGTHARHDGGMRIERERQDY
>Mature_787_residues
STTPSSASADAITEDTVRSPARQYIDAVLEQSFRHLFGPTATPEQPRKHGVVSIANLTAALAEYLGPDEIKEVKAAFHFS
DEAHLGQYRQSGEPYITHPVAVAEICAGWKLDAQAIMAALLHDVMEDQGVTKSELAERFGPKVAELVDGLSKLDKMEFRS
REEAQAENFRKMLLAMARDVRVILVKLADRLHNMRTLGAVPMEKRRRVARETLDIYAPIAHRLGLNNTYRELQDMSFANF
NPHRYATLEKAVKAARGNRREVIGKILEAAQRAMADAKLDAEITGREKTIYSIYRKMRDKQLSFSQVLDVYGFRVVVDSP
LDCYTCIGALHALYKPVPGKFKDYIAIPKINGYQSLHTTLVGPFGAPIEFQVRTRKMHEIAEAGVAAHWLYKNGGADLND
VQKRAHQWLKSLLDIQSEAGDSSEFLEHVKIDLFPDAVYVFTPKSKIMALPRGATALDFAYSIHSDLGNQCVAVKINNEL
LPLRTELKSGDIVEVITAPYSKPNPAWLGFVRTGKARSAIRHYLKTMRLNESVQLGERLVDQSLKGYGLSLADVAPEVWE
KLVQWTGNKSRQEIFADIGLGRRVAAVMAKRIEVLMSGRDADDDLPKGERHVVQHAPPVVITGTEGMSVQLSACCRPIPG
DAIMGYIGIGLGMAIHTTDCRVAQRIHRRDPGRWIDVEWAPQPGRLFDVAVKALVKNTKGIFARVAADITSADANIVHIA
MDEDLTHESTVLRFVIQVSDRVHLANVMRRVRTNPDVMRIMRERSTDDGTHARHDGGMRIERERQDY

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=712, Percent_Identity=45.7865168539326, Blast_Score=660, Evalue=0.0,
Organism=Escherichia coli, GI1789147, Length=683, Percent_Identity=31.7715959004392, Blast_Score=385, Evalue=1e-108,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 87999; Mature: 87868

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTPSSASADAITEDTVRSPARQYIDAVLEQSFRHLFGPTATPEQPRKHGVVSIANLTA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCHHHHHHH
ALAEYLGPDEIKEVKAAFHFSDEAHLGQYRQSGEPYITHPVAVAEICAGWKLDAQAIMAA
HHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEECHHHHHHHHCCCCCCHHHHHHH
LLHDVMEDQGVTKSELAERFGPKVAELVDGLSKLDKMEFRSREEAQAENFRKMLLAMARD
HHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VRVILVKLADRLHNMRTLGAVPMEKRRRVARETLDIYAPIAHRLGLNNTYRELQDMSFAN
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
FNPHRYATLEKAVKAARGNRREVIGKILEAAQRAMADAKLDAEITGREKTIYSIYRKMRD
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHH
KQLSFSQVLDVYGFRVVVDSPLDCYTCIGALHALYKPVPGKFKDYIAIPKINGYQSLHTT
HCCCHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHH
LVGPFGAPIEFQVRTRKMHEIAEAGVAAHWLYKNGGADLNDVQKRAHQWLKSLLDIQSEA
HCCCCCCCEEEEHHHHHHHHHHHCCHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCC
GDSSEFLEHVKIDLFPDAVYVFTPKSKIMALPRGATALDFAYSIHSDLGNQCVAVKINNE
CCHHHHHHHHHHEECCCEEEEECCCHHEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCC
LLPLRTELKSGDIVEVITAPYSKPNPAWLGFVRTGKARSAIRHYLKTMRLNESVQLGERL
EECHHHHCCCCCEEEEEECCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHH
VDQSLKGYGLSLADVAPEVWEKLVQWTGNKSRQEIFADIGLGRRVAAVMAKRIEVLMSGR
HHHHHHCCCCCHHHCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
DADDDLPKGERHVVQHAPPVVITGTEGMSVQLSACCRPIPGDAIMGYIGIGLGMAIHTTD
CCCCCCCCCCHHHHHCCCCEEEECCCCCEEEHHHCCCCCCCHHHHHHHHHHHCCEEECCH
CRVAQRIHRRDPGRWIDVEWAPQPGRLFDVAVKALVKNTKGIFARVAADITSADANIVHI
HHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
AMDEDLTHESTVLRFVIQVSDRVHLANVMRRVRTNPDVMRIMRERSTDDGTHARHDGGMR
EECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCE
IERERQDY
ECHHCCCC
>Mature Secondary Structure 
STTPSSASADAITEDTVRSPARQYIDAVLEQSFRHLFGPTATPEQPRKHGVVSIANLTA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCHHHHHHH
ALAEYLGPDEIKEVKAAFHFSDEAHLGQYRQSGEPYITHPVAVAEICAGWKLDAQAIMAA
HHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEECHHHHHHHHCCCCCCHHHHHHH
LLHDVMEDQGVTKSELAERFGPKVAELVDGLSKLDKMEFRSREEAQAENFRKMLLAMARD
HHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VRVILVKLADRLHNMRTLGAVPMEKRRRVARETLDIYAPIAHRLGLNNTYRELQDMSFAN
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
FNPHRYATLEKAVKAARGNRREVIGKILEAAQRAMADAKLDAEITGREKTIYSIYRKMRD
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHH
KQLSFSQVLDVYGFRVVVDSPLDCYTCIGALHALYKPVPGKFKDYIAIPKINGYQSLHTT
HCCCHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHH
LVGPFGAPIEFQVRTRKMHEIAEAGVAAHWLYKNGGADLNDVQKRAHQWLKSLLDIQSEA
HCCCCCCCEEEEHHHHHHHHHHHCCHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCC
GDSSEFLEHVKIDLFPDAVYVFTPKSKIMALPRGATALDFAYSIHSDLGNQCVAVKINNE
CCHHHHHHHHHHEECCCEEEEECCCHHEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCC
LLPLRTELKSGDIVEVITAPYSKPNPAWLGFVRTGKARSAIRHYLKTMRLNESVQLGERL
EECHHHHCCCCCEEEEEECCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHH
VDQSLKGYGLSLADVAPEVWEKLVQWTGNKSRQEIFADIGLGRRVAAVMAKRIEVLMSGR
HHHHHHCCCCCHHHCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
DADDDLPKGERHVVQHAPPVVITGTEGMSVQLSACCRPIPGDAIMGYIGIGLGMAIHTTD
CCCCCCCCCCHHHHHCCCCEEEECCCCCEEEHHHCCCCCCCHHHHHHHHHHHCCEEECCH
CRVAQRIHRRDPGRWIDVEWAPQPGRLFDVAVKALVKNTKGIFARVAADITSADANIVHI
HHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
AMDEDLTHESTVLRFVIQVSDRVHLANVMRRVRTNPDVMRIMRERSTDDGTHARHDGGMR
EECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCE
IERERQDY
ECHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]