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The map label for this gene is 161525543

Identifier: 161525543

GI number: 161525543

Start: 2585187

End: 2586563

Strand: Direct

Name: 161525543

Synonym: Bmul_2373

Alternate gene names: NA

Gene position: 2585187-2586563 (Clockwise)

Preceding gene: 161525542

Following gene: 161525544

Centisome position: 74.97

GC content: 73.71

Gene sequence:

>1377_bases
ATGACCACTTTCACGCCCTTCCCCCCGCTCGCCCAGCTCGCCGCCGATCTCGCCGCCGGCACGACCACGAGCCGCGCGCT
CGTCGACGCCGCGCTCGAACGGATCGCCGATCCGTCCGGCCAGGGCGCCGTCGTCTTCACCGAAGTCGATGCGGACAACG
CGCGCGCGGCCGCCGACGCACACGACCGGCTGCGGGCCGCGGGCACCGTGCTGTCGCCGCTCGCGGGCATCCCGGTGTCG
ATCAAGGATCTGTTCGACGTCGCCGGACAAGTCACGCGCGCCGGCTCGCGCGTGCTCGACGACGCGCCGCCCGCGCGCGC
CGATGCGGTCGCGGTCGCGCGGGTGCGCCGTGCGGGCGCGGTGCTGGTCGGCCGCACCAACATGAGCGAGTTCGCGTTCT
CGGGGCTCGGGCTCAATCCGCACTTCGGCACGCCCGCATCGCCGTATCGGCGCGACGTGCCGGGCGATGCACGCATCGCG
GGCGGGTCGTCGTCGGGCGCGGCCGCGTCGGTCGCCGACGGGATGGCGGCCGTCGCGCTCGGCACCGACACCGGCGGCTC
GATCCGCATTCCGGCCGCGCTGTGCGGGCTGACCGGCTTCAAGCCGACCGCGAGCCGCGTGCCGACGCAAGGCGGCGTCC
CGCTGTCGACGACGCTCGATTCGTTCGGGCCGATCGGCGTAACGGTCGCATGCTGCGCGCTCGTCGACCGCATGCTCGCC
GGCCTCGAGCCGAGCGTGCCGGCCGCGCGGCCGCTCGAAGGCGTGCGGCTCGGCGTGCCGACGAACTACGTGACCGACGG
CGTCGACGCCGACGTCGCGGCCGCGCTCGATACCGCGCTCAAGCATCTGGAAGCGGCCGGCGCGATCGTCACCGAAGTGC
GGTTCTCCGCGCTCGACCGGCTGCCGGAGATCAACCGCTTCGGCTTCTCGCCGATCGAGGCGTACGCGTGGCACCGTCCG
CTGCTCGCCGAGCATCGCGACCGCTACGACCCGCGCGTGCTGATGCGCATCCTGAAGGGCGAGCCGGCCACCGCGGCCGA
CTATCTCGATCTGCTCGCGGCGCGCCGCGCGATGCTCGACGAAGCCGCGCACACGGTGTGGGCGCGCTTCGATGCGCTGG
TCGCGCCGACGGTGCCGATCGTGCCGCCGCGCATCGCGGAGCTCGAAGCCGACGACGCCGCGTTCACGCGCACCAATGCG
CTGATCCTGCGCAATCCGAGCGCGTTCAACTTCCTCGATGCCTGCGCGCTGTCGCTGCCCTGCCATCCGCGCGACGGCGC
GCCGGTCGGGCTGATGCTCGCGGCCGCGCCGCACCGCGACGATGCGCTGCTCGCGATCGGCCAGTCGGTCGAGGCCGTGC
TGAACACGATCCGCTGA

Upstream 100 bases:

>100_bases
CTGTCCGCGCGCGACGCCGCCGGTGCGCGTGCGGCGGTCGAACGTCATATCGATTCGTTCCGCTCCACGCTTGTCCAACA
TCTTCGCCCCTGAGCCGCTC

Downstream 100 bases:

>100_bases
GCGCGACGCGCGGGGATGCGGCGGGGTGCCGCAGGCGCGGCGGCCGGTGGGGGCCGGACCGTTCGCGCTAAAATCGCGCG
ATCGTTTTCCGTACCGACCG

Product: amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MTTFTPFPPLAQLAADLAAGTTTSRALVDAALERIADPSGQGAVVFTEVDADNARAAADAHDRLRAAGTVLSPLAGIPVS
IKDLFDVAGQVTRAGSRVLDDAPPARADAVAVARVRRAGAVLVGRTNMSEFAFSGLGLNPHFGTPASPYRRDVPGDARIA
GGSSSGAAASVADGMAAVALGTDTGGSIRIPAALCGLTGFKPTASRVPTQGGVPLSTTLDSFGPIGVTVACCALVDRMLA
GLEPSVPAARPLEGVRLGVPTNYVTDGVDADVAAALDTALKHLEAAGAIVTEVRFSALDRLPEINRFGFSPIEAYAWHRP
LLAEHRDRYDPRVLMRILKGEPATAADYLDLLAARRAMLDEAAHTVWARFDALVAPTVPIVPPRIAELEADDAAFTRTNA
LILRNPSAFNFLDACALSLPCHPRDGAPVGLMLAAAPHRDDALLAIGQSVEAVLNTIR

Sequences:

>Translated_458_residues
MTTFTPFPPLAQLAADLAAGTTTSRALVDAALERIADPSGQGAVVFTEVDADNARAAADAHDRLRAAGTVLSPLAGIPVS
IKDLFDVAGQVTRAGSRVLDDAPPARADAVAVARVRRAGAVLVGRTNMSEFAFSGLGLNPHFGTPASPYRRDVPGDARIA
GGSSSGAAASVADGMAAVALGTDTGGSIRIPAALCGLTGFKPTASRVPTQGGVPLSTTLDSFGPIGVTVACCALVDRMLA
GLEPSVPAARPLEGVRLGVPTNYVTDGVDADVAAALDTALKHLEAAGAIVTEVRFSALDRLPEINRFGFSPIEAYAWHRP
LLAEHRDRYDPRVLMRILKGEPATAADYLDLLAARRAMLDEAAHTVWARFDALVAPTVPIVPPRIAELEADDAAFTRTNA
LILRNPSAFNFLDACALSLPCHPRDGAPVGLMLAAAPHRDDALLAIGQSVEAVLNTIR
>Mature_457_residues
TTFTPFPPLAQLAADLAAGTTTSRALVDAALERIADPSGQGAVVFTEVDADNARAAADAHDRLRAAGTVLSPLAGIPVSI
KDLFDVAGQVTRAGSRVLDDAPPARADAVAVARVRRAGAVLVGRTNMSEFAFSGLGLNPHFGTPASPYRRDVPGDARIAG
GSSSGAAASVADGMAAVALGTDTGGSIRIPAALCGLTGFKPTASRVPTQGGVPLSTTLDSFGPIGVTVACCALVDRMLAG
LEPSVPAARPLEGVRLGVPTNYVTDGVDADVAAALDTALKHLEAAGAIVTEVRFSALDRLPEINRFGFSPIEAYAWHRPL
LAEHRDRYDPRVLMRILKGEPATAADYLDLLAARRAMLDEAAHTVWARFDALVAPTVPIVPPRIAELEADDAAFTRTNAL
ILRNPSAFNFLDACALSLPCHPRDGAPVGLMLAAAPHRDDALLAIGQSVEAVLNTIR

Specific function: Unknown

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=313, Percent_Identity=30.6709265175719, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI195972892, Length=493, Percent_Identity=23.7322515212982, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI166795287, Length=138, Percent_Identity=38.4057971014493, Blast_Score=75, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17543272, Length=444, Percent_Identity=27.2522522522523, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17538252, Length=303, Percent_Identity=30.3630363036304, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17537465, Length=502, Percent_Identity=24.7011952191235, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71990152, Length=171, Percent_Identity=36.8421052631579, Blast_Score=88, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17556264, Length=136, Percent_Identity=36.0294117647059, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17538254, Length=232, Percent_Identity=28.8793103448276, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=388, Percent_Identity=28.8659793814433, Blast_Score=110, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6323950, Length=183, Percent_Identity=34.4262295081967, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6320448, Length=169, Percent_Identity=31.9526627218935, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24648113, Length=302, Percent_Identity=33.112582781457, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI45550774, Length=146, Percent_Identity=36.986301369863, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24648435, Length=146, Percent_Identity=36.986301369863, Blast_Score=85, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24648437, Length=146, Percent_Identity=36.986301369863, Blast_Score=85, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24648441, Length=146, Percent_Identity=36.986301369863, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648439, Length=146, Percent_Identity=36.986301369863, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24644968, Length=494, Percent_Identity=25.1012145748988, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21356731, Length=488, Percent_Identity=24.5901639344262, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI161078093, Length=252, Percent_Identity=28.968253968254, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24652985, Length=157, Percent_Identity=33.1210191082803, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19922090, Length=157, Percent_Identity=33.1210191082803, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24652981, Length=157, Percent_Identity=33.1210191082803, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24652983, Length=157, Percent_Identity=33.1210191082803, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.1.4 [H]

Molecular weight: Translated: 47439; Mature: 47308

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTFTPFPPLAQLAADLAAGTTTSRALVDAALERIADPSGQGAVVFTEVDADNARAAADA
CCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHH
HDRLRAAGTVLSPLAGIPVSIKDLFDVAGQVTRAGSRVLDDAPPARADAVAVARVRRAGA
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHCCE
VLVGRTNMSEFAFSGLGLNPHFGTPASPYRRDVPGDARIAGGSSSGAAASVADGMAAVAL
EEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHCCCEEEEE
GTDTGGSIRIPAALCGLTGFKPTASRVPTQGGVPLSTTLDSFGPIGVTVACCALVDRMLA
ECCCCCCEECCHHHHCCCCCCCCHHCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHH
GLEPSVPAARPLEGVRLGVPTNYVTDGVDADVAAALDTALKHLEAAGAIVTEVRFSALDR
CCCCCCCCCCCCCCEEECCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LPEINRFGFSPIEAYAWHRPLLAEHRDRYDPRVLMRILKGEPATAADYLDLLAARRAMLD
CCCHHHCCCCHHHHHHHCCCHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
EAAHTVWARFDALVAPTVPIVPPRIAELEADDAAFTRTNALILRNPSAFNFLDACALSLP
HHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCHHEECCEEEEECCCHHHHHHHHHHCCC
CHPRDGAPVGLMLAAAPHRDDALLAIGQSVEAVLNTIR
CCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTFTPFPPLAQLAADLAAGTTTSRALVDAALERIADPSGQGAVVFTEVDADNARAAADA
CCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHH
HDRLRAAGTVLSPLAGIPVSIKDLFDVAGQVTRAGSRVLDDAPPARADAVAVARVRRAGA
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHCCE
VLVGRTNMSEFAFSGLGLNPHFGTPASPYRRDVPGDARIAGGSSSGAAASVADGMAAVAL
EEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHCCCEEEEE
GTDTGGSIRIPAALCGLTGFKPTASRVPTQGGVPLSTTLDSFGPIGVTVACCALVDRMLA
ECCCCCCEECCHHHHCCCCCCCCHHCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHH
GLEPSVPAARPLEGVRLGVPTNYVTDGVDADVAAALDTALKHLEAAGAIVTEVRFSALDR
CCCCCCCCCCCCCCEEECCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LPEINRFGFSPIEAYAWHRPLLAEHRDRYDPRVLMRILKGEPATAADYLDLLAARRAMLD
CCCHHHCCCCHHHHHHHCCCHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
EAAHTVWARFDALVAPTVPIVPPRIAELEADDAAFTRTNALILRNPSAFNFLDACALSLP
HHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCHHEECCEEEEECCCHHHHHHHHHHCCC
CHPRDGAPVGLMLAAAPHRDDALLAIGQSVEAVLNTIR
CCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9809753 [H]