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The map label for this gene is ynfL [H]

Identifier: 161525516

GI number: 161525516

Start: 2555807

End: 2556748

Strand: Direct

Name: ynfL [H]

Synonym: Bmul_2346

Alternate gene names: 161525516

Gene position: 2555807-2556748 (Clockwise)

Preceding gene: 161525515

Following gene: 161525517

Centisome position: 74.11

GC content: 62.31

Gene sequence:

>942_bases
TTGGAACTTCGCCATCTGCGCTGCTTCCTCGCGGTAGCCTCAGAGCTTCATTTCGCTCGTGCGGCTGAGCGGCTGCATAT
TGAGCAGTCGCCTCTTTCGAGGGCGATTAAGGAGTTGGAAGAGGATCTGGGCGTCAAGCTTTTTGTTCGGACGACTCGCA
GCACGCGGCTGACTCGTGCGGGAAAGCTGTTCTTTGAGCATGTCCCGCGCGTCTTCTCTGCCTTGCAACAGGCCCGTGAC
AGCGTGAAGGCCGCAGCCAACGGCTTTCACAGTCAATTACGCATCGCCCTGTCCGATGGCATCACACCGGCGCGGTTGCC
AGCCTTGCTGGCGCTCTGCCGCCAGGAAGAGCCCGAGGTCGAAATCCGCTTCTTCGAAGTACCTCTGTCCCAACAGATCA
AAGGACTGCATGGCGACCTGTACGACGTGGGCTTTGCCCGCGCCGATGAGGTCGGCGATGGCCTCATTGCCGAATCCGTA
TGGAGTGATCCGTTGATGGTGGCCGTTCCGGCGCGGCATCCCCTGCTGGCACACAAACGCATTCCCTTGGAAGAACTGCT
GCGCTATCCGCTGGTGCTCTGCGATCCGCAGGCATGCGAAGGCCACGCACGGCAGGTTGATCGTGTCCTGCGCCGCGTGG
ATATGGAACCTCTGGTGGCCGAACGGGTGGTGTCCTGCGAACTGATGATGGCGCTGGTGTCCGCAGGCATCGCCCTGGGG
CTGACGGGCTACGCGCACATACTGGCAAGCCAGGAACCTGGCGTAGTGGCGCGGCCACTGGCGGGCCGCGCACCGTTGCT
GACGACCTACCTGCTGCGCCCGGAAGGTGAGCCTTCGGAAATGCTCGCCCGCTTCATTGACCGTGTGCAGGCCATCGAAT
CTCCTGAAGGCAGCAGGCCCATGCCGGGCCTTGATGCCGACTCCCTGGAGGAACCCGCATGA

Upstream 100 bases:

>100_bases
CATTCCTAGAACGCCCCTCTCATGGCTAACGCAAAACGATGTCGATCATGGCCAAAGCGTTCAGATGGTCGAAGGATGCG
ATCACAAAAGGAGATAGCCC

Downstream 100 bases:

>100_bases
TGAAGAACTCCATTCCGTTCCTGCTGGCCGTGGCATTAACCGCCTGCGGTCAATCGGAGATGCCCAAGCAGGCCGACGTG
CCGACCGTGGAAGAACTGGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MELRHLRCFLAVASELHFARAAERLHIEQSPLSRAIKELEEDLGVKLFVRTTRSTRLTRAGKLFFEHVPRVFSALQQARD
SVKAAANGFHSQLRIALSDGITPARLPALLALCRQEEPEVEIRFFEVPLSQQIKGLHGDLYDVGFARADEVGDGLIAESV
WSDPLMVAVPARHPLLAHKRIPLEELLRYPLVLCDPQACEGHARQVDRVLRRVDMEPLVAERVVSCELMMALVSAGIALG
LTGYAHILASQEPGVVARPLAGRAPLLTTYLLRPEGEPSEMLARFIDRVQAIESPEGSRPMPGLDADSLEEPA

Sequences:

>Translated_313_residues
MELRHLRCFLAVASELHFARAAERLHIEQSPLSRAIKELEEDLGVKLFVRTTRSTRLTRAGKLFFEHVPRVFSALQQARD
SVKAAANGFHSQLRIALSDGITPARLPALLALCRQEEPEVEIRFFEVPLSQQIKGLHGDLYDVGFARADEVGDGLIAESV
WSDPLMVAVPARHPLLAHKRIPLEELLRYPLVLCDPQACEGHARQVDRVLRRVDMEPLVAERVVSCELMMALVSAGIALG
LTGYAHILASQEPGVVARPLAGRAPLLTTYLLRPEGEPSEMLARFIDRVQAIESPEGSRPMPGLDADSLEEPA
>Mature_313_residues
MELRHLRCFLAVASELHFARAAERLHIEQSPLSRAIKELEEDLGVKLFVRTTRSTRLTRAGKLFFEHVPRVFSALQQARD
SVKAAANGFHSQLRIALSDGITPARLPALLALCRQEEPEVEIRFFEVPLSQQIKGLHGDLYDVGFARADEVGDGLIAESV
WSDPLMVAVPARHPLLAHKRIPLEELLRYPLVLCDPQACEGHARQVDRVLRRVDMEPLVAERVVSCELMMALVSAGIALG
LTGYAHILASQEPGVVARPLAGRAPLLTTYLLRPEGEPSEMLARFIDRVQAIESPEGSRPMPGLDADSLEEPA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=242, Percent_Identity=34.297520661157, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI2367136, Length=267, Percent_Identity=33.3333333333333, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1788887, Length=295, Percent_Identity=31.864406779661, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI157672245, Length=272, Percent_Identity=31.25, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1790208, Length=256, Percent_Identity=27.734375, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87081904, Length=195, Percent_Identity=29.2307692307692, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790399, Length=199, Percent_Identity=25.6281407035176, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1787601, Length=236, Percent_Identity=24.5762711864407, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1788748, Length=240, Percent_Identity=26.25, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1786713, Length=203, Percent_Identity=33.9901477832512, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI145693105, Length=196, Percent_Identity=26.530612244898, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI48994962, Length=140, Percent_Identity=33.5714285714286, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1787806, Length=303, Percent_Identity=23.4323432343234, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1789173, Length=194, Percent_Identity=32.4742268041237, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1788297, Length=127, Percent_Identity=30.7086614173228, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1787949, Length=148, Percent_Identity=31.7567567567568, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1786401, Length=121, Percent_Identity=36.3636363636364, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1788296, Length=259, Percent_Identity=25.8687258687259, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34615; Mature: 34615

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRHLRCFLAVASELHFARAAERLHIEQSPLSRAIKELEEDLGVKLFVRTTRSTRLTRA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEEEEECCHHHHHHH
GKLFFEHVPRVFSALQQARDSVKAAANGFHSQLRIALSDGITPARLPALLALCRQEEPEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHCCCCHHHHHHHHHHHCCCCCCE
EIRFFEVPLSQQIKGLHGDLYDVGFARADEVGDGLIAESVWSDPLMVAVPARHPLLAHKR
EEEEEECCHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHCCCCEEEEECCCCCCHHCCC
IPLEELLRYPLVLCDPQACEGHARQVDRVLRRVDMEPLVAERVVSCELMMALVSAGIALG
CCHHHHHHCCEEEECCHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LTGYAHILASQEPGVVARPLAGRAPLLTTYLLRPEGEPSEMLARFIDRVQAIESPEGSRP
HHHHHHHHCCCCCCCEECCCCCCCHHHHHHEECCCCCHHHHHHHHHHHHHHHCCCCCCCC
MPGLDADSLEEPA
CCCCCCCCCCCCC
>Mature Secondary Structure
MELRHLRCFLAVASELHFARAAERLHIEQSPLSRAIKELEEDLGVKLFVRTTRSTRLTRA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEEEEECCHHHHHHH
GKLFFEHVPRVFSALQQARDSVKAAANGFHSQLRIALSDGITPARLPALLALCRQEEPEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHCCCCHHHHHHHHHHHCCCCCCE
EIRFFEVPLSQQIKGLHGDLYDVGFARADEVGDGLIAESVWSDPLMVAVPARHPLLAHKR
EEEEEECCHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHCCCCEEEEECCCCCCHHCCC
IPLEELLRYPLVLCDPQACEGHARQVDRVLRRVDMEPLVAERVVSCELMMALVSAGIALG
CCHHHHHHCCEEEECCHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LTGYAHILASQEPGVVARPLAGRAPLLTTYLLRPEGEPSEMLARFIDRVQAIESPEGSRP
HHHHHHHHCCCCCCCEECCCCCCCHHHHHHEECCCCCHHHHHHHHHHHHHHHCCCCCCCC
MPGLDADSLEEPA
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]