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The map label for this gene is vsr [H]

Identifier: 161525454

GI number: 161525454

Start: 2496798

End: 2497241

Strand: Direct

Name: vsr [H]

Synonym: Bmul_2284

Alternate gene names: 161525454

Gene position: 2496798-2497241 (Clockwise)

Preceding gene: 161525453

Following gene: 161525455

Centisome position: 72.4

GC content: 53.15

Gene sequence:

>444_bases
ATGACCGCCAATCCGGAAATTTACAGAAAAGAGGTTGCACAATCGTCGAGGAAGCTGACAAAGTCGGAGCAGATGGCTAG
AGTCAGAAGCCGGAACACGGCTCCCGAGCTAGCACTCCGTCGTGCTCTTTGGCATCAAGGACTCCGATATCGGCTGACGC
CGAAGCTTCCAGGCACACCCGATCTGTGCTTCGTGACTAAACGGGTTGCCATATTCGTGGACGGATGTTTCTGGCACGGT
TGTCCGCAGCATTATTCCGCACCTGCCCAGAACGCGGAATTCTGGTCCCATAAGGTTGCCAGTAATATTACCAGGGACAG
AAAGGCAGATGCAGCATTAGAAGCTGAAGGCTGGACCGTTATGAGGGTTTGGGAACACGAGCTGAAATCGCACTTGGCCG
ACGTGGTGGCGCGGATCGAAGCATTACTTGATGCAAAGAATTGA

Upstream 100 bases:

>100_bases
GATTCCTGCCTGCGCCTTGTCTCCGCACTGCTCGCCGAACTCGACGACGAGTGGATGACCGGCAAGGTCTATCTCAACTT
CAACCCGTAACCCGAATGTC

Downstream 100 bases:

>100_bases
AGTAATCGAGAAAAATAAGAGATCCCGCTCAATAGATTGAAGGAATAAATATTAATGCGTTTCATTGCGGGTCCCCTCAA
CAAGCAACTACTGCAAAACC

Product: DNA mismatch endonuclease Vsr

Products: DNA [C]

Alternate protein names: V.NaeI [H]

Number of amino acids: Translated: 147; Mature: 146

Protein sequence:

>147_residues
MTANPEIYRKEVAQSSRKLTKSEQMARVRSRNTAPELALRRALWHQGLRYRLTPKLPGTPDLCFVTKRVAIFVDGCFWHG
CPQHYSAPAQNAEFWSHKVASNITRDRKADAALEAEGWTVMRVWEHELKSHLADVVARIEALLDAKN

Sequences:

>Translated_147_residues
MTANPEIYRKEVAQSSRKLTKSEQMARVRSRNTAPELALRRALWHQGLRYRLTPKLPGTPDLCFVTKRVAIFVDGCFWHG
CPQHYSAPAQNAEFWSHKVASNITRDRKADAALEAEGWTVMRVWEHELKSHLADVVARIEALLDAKN
>Mature_146_residues
TANPEIYRKEVAQSSRKLTKSEQMARVRSRNTAPELALRRALWHQGLRYRLTPKLPGTPDLCFVTKRVAIFVDGCFWHGC
PQHYSAPAQNAEFWSHKVASNITRDRKADAALEAEGWTVMRVWEHELKSHLADVVARIEALLDAKN

Specific function: May nick NaeI sequences that contain T:G mispairs resulting from m5C-deamination [H]

COG id: COG3727

COG function: function code L; DNA G:T-mismatch repair endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vsr family [H]

Homologues:

Organism=Escherichia coli, GI1788270, Length=121, Percent_Identity=38.0165289256198, Blast_Score=83, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004603
- InterPro:   IPR011335 [H]

Pfam domain/function: PF03852 Vsr [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 16822; Mature: 16691

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTANPEIYRKEVAQSSRKLTKSEQMARVRSRNTAPELALRRALWHQGLRYRLTPKLPGTP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEECCCCCCCC
DLCFVTKRVAIFVDGCFWHGCPQHYSAPAQNAEFWSHKVASNITRDRKADAALEAEGWTV
CHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
MRVWEHELKSHLADVVARIEALLDAKN
HHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TANPEIYRKEVAQSSRKLTKSEQMARVRSRNTAPELALRRALWHQGLRYRLTPKLPGTP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEECCCCCCCC
DLCFVTKRVAIFVDGCFWHGCPQHYSAPAQNAEFWSHKVASNITRDRKADAALEAEGWTV
CHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
MRVWEHELKSHLADVVARIEALLDAKN
HHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: DNA mismatch endonuclease vsr specifically recognizes a T-G mismatch in a special context related to the dcm methylation sequence and makes an incision 5' to the mismatched T residue [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7698663 [H]