Click here to switch to the map view.

The map label for this gene is gph2 [H]

Identifier: 161525437

GI number: 161525437

Start: 2482753

End: 2483469

Strand: Direct

Name: gph2 [H]

Synonym: Bmul_2267

Alternate gene names: 161525437

Gene position: 2482753-2483469 (Clockwise)

Preceding gene: 161525436

Following gene: 161525440

Centisome position: 72.0

GC content: 71.97

Gene sequence:

>717_bases
ATGACGACTCCCCTTTCGCCCGCGCGGGACGTGCTCGACGCACCGCGCCTGCAGCACTGCGAAGCCGTGCTGTTCGACCT
CGACGGCACGCTCGCCGATACGGCGCCCGATCTCGCGGCGGCCGTCAACAAGATGCAGCGCGTGCGCGGCCTGCCGGAAA
CGCCGCTCGACGCGCTGCGGCGCCTCGCGTCCGCGGGCGCGCGCGGCCTGCTCGGCGGCGCGTTCGGCATCACGCCCGAG
ACGCCCGGCTACGATGCGATGCGCGACGAGTTCCTCGCGAACTACGCCGCCGATATTTGCGTTCAGACGACGCTGTTCCC
CGGCATTGCCGAAGTGCTCGACGAACTCGAAGCACGCGGCGTGCGCTGGGGGATCGTCACGAACAAGGCGATGCGATTGA
CCGCGCCGCTCGTCGAATTGCTGGGCCTCGCGTCGCGCGCGGCCTGCGTGGTGGGGGGCGACACGACGCCGCACCCGAAG
CCGCATCCCGCTCCGCTGCTGCACGCGGCCGGCCTGCTCGCACTGGCGCCGGACCGCATCGTCTACGTTGGCGACGACCT
GCGCGACATTCAGGCCGGCTACGCAGCCGGGATGGCGACGGTCGCGGCCGCGTACGGCTACTGCGGCGACGGCGCCGCGC
CGGGCGACTGGCGTGCGCAGCACCTCGTCGGCACGACCGGCGAGCTGCGCGATCTGCTGCGCGATGTTGCGCTATAA

Upstream 100 bases:

>100_bases
ATCACCTATCATCCGCTCGGCAAGCGCTTCGCGCTGTCGAACGACACCGACATCAACTATCTGGTCGCGTGCCGCCGCGC
CGCGTAACCTGCCGACCCGC

Downstream 100 bases:

>100_bases
TGATCAATCTTATCCGCGGGGGCGACCTGGTTTCGACAGGGGTTGTGAAGCGGCTAGGGCATGTCGAGGACCCGTCACCT
CGTTAATCAATGGGAAAAAC

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: PGP 2; PGPase2 [H]

Number of amino acids: Translated: 238; Mature: 237

Protein sequence:

>238_residues
MTTPLSPARDVLDAPRLQHCEAVLFDLDGTLADTAPDLAAAVNKMQRVRGLPETPLDALRRLASAGARGLLGGAFGITPE
TPGYDAMRDEFLANYAADICVQTTLFPGIAEVLDELEARGVRWGIVTNKAMRLTAPLVELLGLASRAACVVGGDTTPHPK
PHPAPLLHAAGLLALAPDRIVYVGDDLRDIQAGYAAGMATVAAAYGYCGDGAAPGDWRAQHLVGTTGELRDLLRDVAL

Sequences:

>Translated_238_residues
MTTPLSPARDVLDAPRLQHCEAVLFDLDGTLADTAPDLAAAVNKMQRVRGLPETPLDALRRLASAGARGLLGGAFGITPE
TPGYDAMRDEFLANYAADICVQTTLFPGIAEVLDELEARGVRWGIVTNKAMRLTAPLVELLGLASRAACVVGGDTTPHPK
PHPAPLLHAAGLLALAPDRIVYVGDDLRDIQAGYAAGMATVAAAYGYCGDGAAPGDWRAQHLVGTTGELRDLLRDVAL
>Mature_237_residues
TTPLSPARDVLDAPRLQHCEAVLFDLDGTLADTAPDLAAAVNKMQRVRGLPETPLDALRRLASAGARGLLGGAFGITPET
PGYDAMRDEFLANYAADICVQTTLFPGIAEVLDELEARGVRWGIVTNKAMRLTAPLVELLGLASRAACVVGGDTTPHPKP
HPAPLLHAAGLLALAPDRIVYVGDDLRDIQAGYAAGMATVAAAYGYCGDGAAPGDWRAQHLVGTTGELRDLLRDVAL

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=212, Percent_Identity=34.4339622641509, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 24941; Mature: 24810

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPLSPARDVLDAPRLQHCEAVLFDLDGTLADTAPDLAAAVNKMQRVRGLPETPLDALR
CCCCCCHHHHHHCCCHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHCCCCCCHHHHHH
RLASAGARGLLGGAFGITPETPGYDAMRDEFLANYAADICVQTTLFPGIAEVLDELEARG
HHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VRWGIVTNKAMRLTAPLVELLGLASRAACVVGGDTTPHPKPHPAPLLHAAGLLALAPDRI
CEEEEECCCHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCE
VYVGDDLRDIQAGYAAGMATVAAAYGYCGDGAAPGDWRAQHLVGTTGELRDLLRDVAL
EEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TTPLSPARDVLDAPRLQHCEAVLFDLDGTLADTAPDLAAAVNKMQRVRGLPETPLDALR
CCCCCHHHHHHCCCHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHCCCCCCHHHHHH
RLASAGARGLLGGAFGITPETPGYDAMRDEFLANYAADICVQTTLFPGIAEVLDELEARG
HHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VRWGIVTNKAMRLTAPLVELLGLASRAACVVGGDTTPHPKPHPAPLLHAAGLLALAPDRI
CEEEEECCCHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCE
VYVGDDLRDIQAGYAAGMATVAAAYGYCGDGAAPGDWRAQHLVGTTGELRDLLRDVAL
EEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]