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The map label for this gene is dfrA [H]

Identifier: 161525391

GI number: 161525391

Start: 2437475

End: 2438104

Strand: Direct

Name: dfrA [H]

Synonym: Bmul_2221

Alternate gene names: 161525391

Gene position: 2437475-2438104 (Clockwise)

Preceding gene: 161525390

Following gene: 161525393

Centisome position: 70.68

GC content: 67.14

Gene sequence:

>630_bases
ATGCAACGGTTTCGAGCCCGTCCCCCGGCGGGCATTTTTTTGTCTGCACGCAGGCGCAGCACGCCGCGAGGAGGCGCGCC
CGTTCCGGTAGAATCGGCGCCATTCCGGTTCTTCCTCTCTCCGACCTTCATGACGACGTTGACCCTGATCGTCGCGCGCG
CCCGCAACGGCATCATCGGCCGCGACAACCAGCTGCCCTGGAAACTTCCCGAAGACCTCGCGTTCTTCAAGCGCACGACG
ATGGGCGCGCCGATCGTGATGGGACGCAAGACGCACGAGTCGATCGGCCGTCCGCTGCCGGGCCGCCGCAACATCGTCGT
CACGCGCGACGCGTCGCGCCGCTTCGACGGCTGCGACACCGTCACGTCGCTTGCCGATGCGCTCGCGCTCGCCGCGCGCG
ACGGTGCCGCGGAAGCATTCCTGATCGGCGGCGCGCAACTGTATGCGGAAGGCCTCGCCCAGGCGGACAAGCTGATCGTC
ACCGAGATCGATGCCGATTTCGAAGGCGACGCATCGTTTCCGGCGCCCGATCCCGCGCAGTGGCAGGAAGTGTCGCGCGA
TGCGCATCAGGCTGCCACGCCGAACACGTTCGGCTACGCATTCGTCGTCTACGCACGCAAGCGCGGCTGA

Upstream 100 bases:

>100_bases
ATGAAACGTCGGCGCCGATCGCACGGCAGTCGATCAGGATTGCGTATCGATCGTCTGTCCGGTATCGGCAACACGATCGA
TCGACATCGCCACCTCGGCG

Downstream 100 bases:

>100_bases
TACGTACCGCCCGATGAAAAAAACCGACCGGCTCGCGCCGATCGGTTTTTTTTCGTCCGAGCGCGCCGCCGACGCGGCGC
AGTGCCGTGCTTACTGGCCG

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MQRFRARPPAGIFLSARRRSTPRGGAPVPVESAPFRFFLSPTFMTTLTLIVARARNGIIGRDNQLPWKLPEDLAFFKRTT
MGAPIVMGRKTHESIGRPLPGRRNIVVTRDASRRFDGCDTVTSLADALALAARDGAAEAFLIGGAQLYAEGLAQADKLIV
TEIDADFEGDASFPAPDPAQWQEVSRDAHQAATPNTFGYAFVVYARKRG

Sequences:

>Translated_209_residues
MQRFRARPPAGIFLSARRRSTPRGGAPVPVESAPFRFFLSPTFMTTLTLIVARARNGIIGRDNQLPWKLPEDLAFFKRTT
MGAPIVMGRKTHESIGRPLPGRRNIVVTRDASRRFDGCDTVTSLADALALAARDGAAEAFLIGGAQLYAEGLAQADKLIV
TEIDADFEGDASFPAPDPAQWQEVSRDAHQAATPNTFGYAFVVYARKRG
>Mature_209_residues
MQRFRARPPAGIFLSARRRSTPRGGAPVPVESAPFRFFLSPTFMTTLTLIVARARNGIIGRDNQLPWKLPEDLAFFKRTT
MGAPIVMGRKTHESIGRPLPGRRNIVVTRDASRRFDGCDTVTSLADALALAARDGAAEAFLIGGAQLYAEGLAQADKLIV
TEIDADFEGDASFPAPDPAQWQEVSRDAHQAATPNTFGYAFVVYARKRG

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=182, Percent_Identity=31.3186813186813, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1786233, Length=163, Percent_Identity=41.1042944785276, Blast_Score=135, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17505899, Length=183, Percent_Identity=30.6010928961749, Blast_Score=77, Evalue=7e-15,
Organism=Drosophila melanogaster, GI116008026, Length=160, Percent_Identity=33.125, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24647458, Length=160, Percent_Identity=33.125, Blast_Score=71, Evalue=5e-13,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 22804; Mature: 22804

Theoretical pI: Translated: 10.71; Mature: 10.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRFRARPPAGIFLSARRRSTPRGGAPVPVESAPFRFFLSPTFMTTLTLIVARARNGIIG
CCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCC
RDNQLPWKLPEDLAFFKRTTMGAPIVMGRKTHESIGRPLPGRRNIVVTRDASRRFDGCDT
CCCCCCCCCCHHHHHHHHHCCCCCEEECCCCHHHHCCCCCCCCCEEEEECCCCCCCCHHH
VTSLADALALAARDGAAEAFLIGGAQLYAEGLAQADKLIVTEIDADFEGDASFPAPDPAQ
HHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCHH
WQEVSRDAHQAATPNTFGYAFVVYARKRG
HHHHHHHHHHHCCCCCCCEEEEEEEECCC
>Mature Secondary Structure
MQRFRARPPAGIFLSARRRSTPRGGAPVPVESAPFRFFLSPTFMTTLTLIVARARNGIIG
CCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCC
RDNQLPWKLPEDLAFFKRTTMGAPIVMGRKTHESIGRPLPGRRNIVVTRDASRRFDGCDT
CCCCCCCCCCHHHHHHHHHCCCCCEEECCCCHHHHCCCCCCCCCEEEEECCCCCCCCHHH
VTSLADALALAARDGAAEAFLIGGAQLYAEGLAQADKLIVTEIDADFEGDASFPAPDPAQ
HHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCHH
WQEVSRDAHQAATPNTFGYAFVVYARKRG
HHHHHHHHHHHCCCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840350; 9384377 [H]