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The map label for this gene is rluB [H]

Identifier: 161524931

GI number: 161524931

Start: 1946950

End: 1948614

Strand: Reverse

Name: rluB [H]

Synonym: Bmul_1758

Alternate gene names: 161524931

Gene position: 1948614-1946950 (Counterclockwise)

Preceding gene: 161524932

Following gene: 161524930

Centisome position: 56.51

GC content: 73.57

Gene sequence:

>1665_bases
TTGACAGATATCCACGATATTGAATCGTCCGCGCCCGCCGTGCAGGCTGCGCGCGCCGACGATGCGCCGGAGCAGGACGC
TCCGGCCGCGGGTGGCGACGAACGTCCCCGCCGCGGCCTGCGGCGCGGTCCGCGCAGCCTGATCGCGCGCCGTCGCGCAG
CGGCCAAGTCGAAGGGCGCCGACGCGGCGCCGCAAGACGGCGAAGCCGCCGATGCGCCGCCGGCCGACGCCGTGCAGGCG
CAGCCGGCGCGTGCGCCGCGCAAGGAAGGCGCGGCCAAGGGCGGGCGCGGCAAGTCCGGCGGCAAGCGCGAAGGCGCGGG
CGGCAAGGGCGCGCAGGGTCAAGCGCAAGGCCAGGCCAAGCGCGGCGGCGCGTCGAAGGGCGAAGGCGAAGCGGCGCAGG
ACGACCTGTTCGCCTACGTGACGTCGCCGGCGTTCGACGCGGACAACGCGGCACCGGGCAGCGGCGTGCGTGCGCCGATG
CTGCGCCGCGGCCGCGGTCAGCCGGCGAACAAGCGCGTGCTGTCGCCGGACGACGACGCGCCGAAGCTGCACAAGGTGCT
CGCGGAAGCGGGGATGGGCTCGCGCCGCGAAATGGAAGAGCTGATCATCGCCGGCCGCGTGTCGGTGAACGGCGAACCCG
CGCATATCGGCCAGCGCATCATGCCGACCGACCAGGTGCGGATCAACGGCAAGCCGGTCAAGCGCAAGCTGCCGAACAAG
CCGCCGCGCGTGCTGCTCTACCACAAGCCGACCGGCGAGATCGTCAGTCACGCGGATCCGGAAGGCCGTCCGTCGGTGTT
CGACCGCCTGCCGCCGATGAAGACGGCGAAATGGCTCGCGGTCGGCCGCCTCGACTTCAATACCGAAGGTCTGCTGATGC
TGACGACCTCCGGCGATCTCGCGAACCGCTTCATGCATCCGCGCTACAGCGTCGAGCGCGAGTACGCGGTGCGCGTGGTC
GGCGAGCTGTCGGAAAGCGCGCGCCAGAAGCTGCTGCACGGCATCGAGCTCGACGACGGTCCCGCGAACTTCCTGCGCAT
CCGCGACGGCGGCGGCGAGGGCACGAATCACTGGTATCACGTCGCGCTGGCCGAAGGCCGTAACCGCGAAGTGCGCCGGA
TGTTCGAGGCGGTCGGCCTGATGGTGAGCCGCCTGATCCGCACGCGTCACGGCCCGATTCCGCTGCCGCGCGGCCTGAAG
CGCGGCCGCTGGGAGGAGCTCGACGAGGCGCAGGTGCGCAAGCTGATGGCGACGGTCGGCCTGAAGGCGCCGTCGGAAGA
GAAGGGCAAGCGCGGCGGCGGCCAGGCCGAGCGCCGGCAGCCCGATCCGATGCAGACGTCGATGGGCTTCATCAGCCGCG
AGCCGGTGCTGACGACCCACGGCCAGCTCGACCAGCCGCGCCGCGGCGGTCGGCGCGGCGGCCCGGGCCTGCCGGGCCTG
AGCGGCTACGGCAGCCTGCCGGTCGCGTCGGGGTACGGCAATCGCGCGGGCGGCCGCGACGTCGACGGCAATCGCGCGTC
GCATGGCGGCGCGGGCGGCAAGCGCGGCGGCGGCAAGGGCGGCAATCGCAACCCGAACGGCAATCGCGCGGATGGCGGCG
CCGGCAACGGCGGCCCGCGCGGCGGCCAGCGTCCGCAGCGCGGCGGGCGTTCGCGCGGCCGCTGA

Upstream 100 bases:

>100_bases
GCAGGACGACGAAGCAGAACCCGAACAGCGTCGCGCCTGATCGCGGCTGACTTCTTGCCGCGCCCGTTCCGGGCGCGAGA
CCTGACATTTTGAGGTTGTT

Downstream 100 bases:

>100_bases
GCGGCGCGTTGCGCGAGGCGCGACAGCGCCGGCAAGACCGGGTCGATCCGGTTTTGTCGGCGCATTGCATTTTGCGCAAT
CGACGCTATATGCATAGTCG

Product: pseudouridine synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 554; Mature: 553

Protein sequence:

>554_residues
MTDIHDIESSAPAVQAARADDAPEQDAPAAGGDERPRRGLRRGPRSLIARRRAAAKSKGADAAPQDGEAADAPPADAVQA
QPARAPRKEGAAKGGRGKSGGKREGAGGKGAQGQAQGQAKRGGASKGEGEAAQDDLFAYVTSPAFDADNAAPGSGVRAPM
LRRGRGQPANKRVLSPDDDAPKLHKVLAEAGMGSRREMEELIIAGRVSVNGEPAHIGQRIMPTDQVRINGKPVKRKLPNK
PPRVLLYHKPTGEIVSHADPEGRPSVFDRLPPMKTAKWLAVGRLDFNTEGLLMLTTSGDLANRFMHPRYSVEREYAVRVV
GELSESARQKLLHGIELDDGPANFLRIRDGGGEGTNHWYHVALAEGRNREVRRMFEAVGLMVSRLIRTRHGPIPLPRGLK
RGRWEELDEAQVRKLMATVGLKAPSEEKGKRGGGQAERRQPDPMQTSMGFISREPVLTTHGQLDQPRRGGRRGGPGLPGL
SGYGSLPVASGYGNRAGGRDVDGNRASHGGAGGKRGGGKGGNRNPNGNRADGGAGNGGPRGGQRPQRGGRSRGR

Sequences:

>Translated_554_residues
MTDIHDIESSAPAVQAARADDAPEQDAPAAGGDERPRRGLRRGPRSLIARRRAAAKSKGADAAPQDGEAADAPPADAVQA
QPARAPRKEGAAKGGRGKSGGKREGAGGKGAQGQAQGQAKRGGASKGEGEAAQDDLFAYVTSPAFDADNAAPGSGVRAPM
LRRGRGQPANKRVLSPDDDAPKLHKVLAEAGMGSRREMEELIIAGRVSVNGEPAHIGQRIMPTDQVRINGKPVKRKLPNK
PPRVLLYHKPTGEIVSHADPEGRPSVFDRLPPMKTAKWLAVGRLDFNTEGLLMLTTSGDLANRFMHPRYSVEREYAVRVV
GELSESARQKLLHGIELDDGPANFLRIRDGGGEGTNHWYHVALAEGRNREVRRMFEAVGLMVSRLIRTRHGPIPLPRGLK
RGRWEELDEAQVRKLMATVGLKAPSEEKGKRGGGQAERRQPDPMQTSMGFISREPVLTTHGQLDQPRRGGRRGGPGLPGL
SGYGSLPVASGYGNRAGGRDVDGNRASHGGAGGKRGGGKGGNRNPNGNRADGGAGNGGPRGGQRPQRGGRSRGR
>Mature_553_residues
TDIHDIESSAPAVQAARADDAPEQDAPAAGGDERPRRGLRRGPRSLIARRRAAAKSKGADAAPQDGEAADAPPADAVQAQ
PARAPRKEGAAKGGRGKSGGKREGAGGKGAQGQAQGQAKRGGASKGEGEAAQDDLFAYVTSPAFDADNAAPGSGVRAPML
RRGRGQPANKRVLSPDDDAPKLHKVLAEAGMGSRREMEELIIAGRVSVNGEPAHIGQRIMPTDQVRINGKPVKRKLPNKP
PRVLLYHKPTGEIVSHADPEGRPSVFDRLPPMKTAKWLAVGRLDFNTEGLLMLTTSGDLANRFMHPRYSVEREYAVRVVG
ELSESARQKLLHGIELDDGPANFLRIRDGGGEGTNHWYHVALAEGRNREVRRMFEAVGLMVSRLIRTRHGPIPLPRGLKR
GRWEELDEAQVRKLMATVGLKAPSEEKGKRGGGQAERRQPDPMQTSMGFISREPVLTTHGQLDQPRRGGRRGGPGLPGLS
GYGSLPVASGYGNRAGGRDVDGNRASHGGAGGKRGGGKGGNRNPNGNRADGGAGNGGPRGGQRPQRGGRSRGR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=245, Percent_Identity=56.734693877551, Blast_Score=274, Evalue=1e-74,
Organism=Escherichia coli, GI1790453, Length=253, Percent_Identity=29.2490118577075, Blast_Score=99, Evalue=9e-22,
Organism=Escherichia coli, GI87081838, Length=193, Percent_Identity=34.1968911917098, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788510, Length=218, Percent_Identity=29.3577981651376, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 58676; Mature: 58545

Theoretical pI: Translated: 11.29; Mature: 11.29

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDIHDIESSAPAVQAARADDAPEQDAPAAGGDERPRRGLRRGPRSLIARRRAAAKSKGA
CCCCHHHHCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHCCCC
DAAPQDGEAADAPPADAVQAQPARAPRKEGAAKGGRGKSGGKREGAGGKGAQGQAQGQAK
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
RGGASKGEGEAAQDDLFAYVTSPAFDADNAAPGSGVRAPMLRRGRGQPANKRVLSPDDDA
CCCCCCCCCCCHHHCEEEEECCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCC
PKLHKVLAEAGMGSRREMEELIIAGRVSVNGEPAHIGQRIMPTDQVRINGKPVKRKLPNK
HHHHHHHHHHCCCCHHHHHHHHHHCEEECCCCHHHHCCCCCCCCEEEECCCCHHHHCCCC
PPRVLLYHKPTGEIVSHADPEGRPSVFDRLPPMKTAKWLAVGRLDFNTEGLLMLTTSGDL
CCEEEEEECCCCHHHHCCCCCCCCHHHHCCCCCCCCCEEEEECEEECCCCEEEEECCCHH
ANRFMHPRYSVEREYAVRVVGELSESARQKLLHGIELDDGPANFLRIRDGGGEGTNHWYH
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCEEE
VALAEGRNREVRRMFEAVGLMVSRLIRTRHGPIPLPRGLKRGRWEELDEAQVRKLMATVG
EEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
LKAPSEEKGKRGGGQAERRQPDPMQTSMGFISREPVLTTHGQLDQPRRGGRRGGPGLPGL
CCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCCC
SGYGSLPVASGYGNRAGGRDVDGNRASHGGAGGKRGGGKGGNRNPNGNRADGGAGNGGPR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GGQRPQRGGRSRGR
CCCCCCCCCCCCCC
>Mature Secondary Structure 
TDIHDIESSAPAVQAARADDAPEQDAPAAGGDERPRRGLRRGPRSLIARRRAAAKSKGA
CCCHHHHCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHCCCC
DAAPQDGEAADAPPADAVQAQPARAPRKEGAAKGGRGKSGGKREGAGGKGAQGQAQGQAK
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
RGGASKGEGEAAQDDLFAYVTSPAFDADNAAPGSGVRAPMLRRGRGQPANKRVLSPDDDA
CCCCCCCCCCCHHHCEEEEECCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCC
PKLHKVLAEAGMGSRREMEELIIAGRVSVNGEPAHIGQRIMPTDQVRINGKPVKRKLPNK
HHHHHHHHHHCCCCHHHHHHHHHHCEEECCCCHHHHCCCCCCCCEEEECCCCHHHHCCCC
PPRVLLYHKPTGEIVSHADPEGRPSVFDRLPPMKTAKWLAVGRLDFNTEGLLMLTTSGDL
CCEEEEEECCCCHHHHCCCCCCCCHHHHCCCCCCCCCEEEEECEEECCCCEEEEECCCHH
ANRFMHPRYSVEREYAVRVVGELSESARQKLLHGIELDDGPANFLRIRDGGGEGTNHWYH
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCEEE
VALAEGRNREVRRMFEAVGLMVSRLIRTRHGPIPLPRGLKRGRWEELDEAQVRKLMATVG
EEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
LKAPSEEKGKRGGGQAERRQPDPMQTSMGFISREPVLTTHGQLDQPRRGGRRGGPGLPGL
CCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCCC
SGYGSLPVASGYGNRAGGRDVDGNRASHGGAGGKRGGGKGGNRNPNGNRADGGAGNGGPR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GGQRPQRGGRSRGR
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]