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The map label for this gene is sucB [H]

Identifier: 161524919

GI number: 161524919

Start: 1928372

End: 1929664

Strand: Reverse

Name: sucB [H]

Synonym: Bmul_1746

Alternate gene names: 161524919

Gene position: 1929664-1928372 (Counterclockwise)

Preceding gene: 161524920

Following gene: 161524918

Centisome position: 55.96

GC content: 66.9

Gene sequence:

>1293_bases
ATGGCTATCGTAGAAGTCAAAGTCCCCCAGCTTTCGGAGTCGGTGTCGGAAGCCACCATGCTGCAGTGGAAGAAGAAGCC
GGGCGAAGCAGTCGCGCAGGACGAAATCCTGATCGAACTCGAGACCGACAAGGTCGTGCTCGAAGTGCCGGCGCCGGCCG
CGGGCGTGCTCGCGCAAGTGCTGCAGAACGACGGTGACACGGTGGTCGCCGATCAGGTGATCGCGACGATCGACACCGAA
GCGAAGGCGGGCGCCGCCGAAGCCGCGGCAGGCGCAGCCGAAGTCAAGCCGGCCGCCGCACCCGCAGCGGCACCGGCCGC
TCAGCCGGCCGCCGCGACGGCTGCCGCGTCGACGGCCACCGCTTCGTCGGGCACGGCCTCGCCGGCCGCAGCGAAGCTGC
TGGCCGAGAAGGGCCTGTCGGCAGGCGACGTCGCCGGCTCGGGCCGCGACGGCCGCGTCACGAAGGGCGACGCGCTCGCC
GCCGGCAGCGCGCCGAAGGCGGCTGCTCCGGCCGCCGCACCGGCAAAGGCCGCGGCCAAGCCGGCGCTGCCGGAAGTGAA
GGTGCCGGCGTCGGCAACGACCTGGCTGAACGACCGTCCGGAACAACGCGTGCCGATGTCGCGCCTGCGTGCGCGTATCG
CCGAGCGTCTGCTCGAGTCGCAGCAGACCAACGCGATCCTGACGACGTTCAACGAAGTCAACATGCAGCCGGTCATGGAC
CTGCGCGCGAAGTACAAGGACAAGTTCGAGAAGGAACACGGCGTGAAGCTCGGCTTCATGTCGTTCTTCGTGAAGGCGGC
CGTGCATGCGCTGAAGAAGTTCCCGCTCGTGAACGCGTCGATCGACGGCAACGACATCGTCTACCACGGCTACTTCGACA
TCGGTATCGCGGTCGGTTCGCCGCGCGGCCTCGTCGTGCCGATCCTGCGCAACGCCGATCAGCTGAGCCTCGCCGAGATC
GAGAAGAAGATCGCCGAATTCGGCCAGAAGGCGAAGGACGGCAAGCTGTCGATCGAGGAAATGACGGGCGGTACGTTCTC
GATCTCGAACGGCGGCGTGTTCGGCTCGATGCTGTCGACCCCGATCATCAACCCGCCGCAGTCGGCAATCCTCGGCGTGC
ACGCGACGAAGGAGCGTCCGGTCGTCGAAAACGGCCAGATCGTGATCCGTCCGATCAACTACCTCGCGCTGTCGTACGAC
CACCGCATCATCGACGGCCGCGAAGCCGTGCTGTCGCTCGTCGCGATGAAGGATGCGCTGGAAGATCCGGCACGTCTGCT
GCTCGACCTGTAA

Upstream 100 bases:

>100_bases
TCCCGTCTCTTTTGGCCTGCCGCGCGCGCCGCGCGCCGCATCGGCCGAAAGAGCCGCAGGAATCTCGTCATTACCCAGAT
TAAGCATCCAGGAAAATCAC

Downstream 100 bases:

>100_bases
GCCGAGTCTCACCGCATTGACCCGCTCCGCACGGGCGCGCCACCGCGCGTCCGTGCGGATGCACAAGTAGAAAGGATTGC
CATGTCCAAGGAATTTGACG

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MAIVEVKVPQLSESVSEATMLQWKKKPGEAVAQDEILIELETDKVVLEVPAPAAGVLAQVLQNDGDTVVADQVIATIDTE
AKAGAAEAAAGAAEVKPAAAPAAAPAAQPAAATAAASTATASSGTASPAAAKLLAEKGLSAGDVAGSGRDGRVTKGDALA
AGSAPKAAAPAAAPAKAAAKPALPEVKVPASATTWLNDRPEQRVPMSRLRARIAERLLESQQTNAILTTFNEVNMQPVMD
LRAKYKDKFEKEHGVKLGFMSFFVKAAVHALKKFPLVNASIDGNDIVYHGYFDIGIAVGSPRGLVVPILRNADQLSLAEI
EKKIAEFGQKAKDGKLSIEEMTGGTFSISNGGVFGSMLSTPIINPPQSAILGVHATKERPVVENGQIVIRPINYLALSYD
HRIIDGREAVLSLVAMKDALEDPARLLLDL

Sequences:

>Translated_430_residues
MAIVEVKVPQLSESVSEATMLQWKKKPGEAVAQDEILIELETDKVVLEVPAPAAGVLAQVLQNDGDTVVADQVIATIDTE
AKAGAAEAAAGAAEVKPAAAPAAAPAAQPAAATAAASTATASSGTASPAAAKLLAEKGLSAGDVAGSGRDGRVTKGDALA
AGSAPKAAAPAAAPAKAAAKPALPEVKVPASATTWLNDRPEQRVPMSRLRARIAERLLESQQTNAILTTFNEVNMQPVMD
LRAKYKDKFEKEHGVKLGFMSFFVKAAVHALKKFPLVNASIDGNDIVYHGYFDIGIAVGSPRGLVVPILRNADQLSLAEI
EKKIAEFGQKAKDGKLSIEEMTGGTFSISNGGVFGSMLSTPIINPPQSAILGVHATKERPVVENGQIVIRPINYLALSYD
HRIIDGREAVLSLVAMKDALEDPARLLLDL
>Mature_429_residues
AIVEVKVPQLSESVSEATMLQWKKKPGEAVAQDEILIELETDKVVLEVPAPAAGVLAQVLQNDGDTVVADQVIATIDTEA
KAGAAEAAAGAAEVKPAAAPAAAPAAQPAAATAAASTATASSGTASPAAAKLLAEKGLSAGDVAGSGRDGRVTKGDALAA
GSAPKAAAPAAAPAKAAAKPALPEVKVPASATTWLNDRPEQRVPMSRLRARIAERLLESQQTNAILTTFNEVNMQPVMDL
RAKYKDKFEKEHGVKLGFMSFFVKAAVHALKKFPLVNASIDGNDIVYHGYFDIGIAVGSPRGLVVPILRNADQLSLAEIE
KKIAEFGQKAKDGKLSIEEMTGGTFSISNGGVFGSMLSTPIINPPQSAILGVHATKERPVVENGQIVIRPINYLALSYDH
RIIDGREAVLSLVAMKDALEDPARLLLDL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=234, Percent_Identity=56.4102564102564, Blast_Score=285, Evalue=4e-77,
Organism=Homo sapiens, GI31711992, Length=443, Percent_Identity=31.1512415349887, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI110671329, Length=441, Percent_Identity=27.6643990929705, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI203098753, Length=458, Percent_Identity=30.5676855895196, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI203098816, Length=458, Percent_Identity=30.5676855895196, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=37.888198757764, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1786946, Length=430, Percent_Identity=52.7906976744186, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI1786305, Length=430, Percent_Identity=32.3255813953488, Blast_Score=165, Evalue=6e-42,
Organism=Caenorhabditis elegans, GI25146366, Length=435, Percent_Identity=42.7586206896552, Blast_Score=325, Evalue=3e-89,
Organism=Caenorhabditis elegans, GI17560088, Length=452, Percent_Identity=32.3008849557522, Blast_Score=183, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17537937, Length=450, Percent_Identity=26.8888888888889, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17538894, Length=316, Percent_Identity=32.9113924050633, Blast_Score=142, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6320352, Length=427, Percent_Identity=43.7939110070258, Blast_Score=332, Evalue=6e-92,
Organism=Saccharomyces cerevisiae, GI6324258, Length=454, Percent_Identity=30.6167400881057, Blast_Score=152, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24645909, Length=242, Percent_Identity=55.3719008264463, Blast_Score=284, Evalue=1e-76,
Organism=Drosophila melanogaster, GI18859875, Length=444, Percent_Identity=28.8288288288288, Blast_Score=152, Evalue=6e-37,
Organism=Drosophila melanogaster, GI20129315, Length=228, Percent_Identity=34.6491228070175, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24582497, Length=228, Percent_Identity=34.6491228070175, Blast_Score=132, Evalue=4e-31,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 44718; Mature: 44586

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIVEVKVPQLSESVSEATMLQWKKKPGEAVAQDEILIELETDKVVLEVPAPAAGVLAQV
CEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCEEEEECCCCHHHHHHHH
LQNDGDTVVADQVIATIDTEAKAGAAEAAAGAAEVKPAAAPAAAPAAQPAAATAAASTAT
HHCCCCEEEHHHHHHEECCCCCCCCHHHHCCCHHCCCCCCCCCCCCCCCHHHHHHHHHCC
ASSGTASPAAAKLLAEKGLSAGDVAGSGRDGRVTKGDALAAGSAPKAAAPAAAPAKAAAK
CCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCHHHHCC
PALPEVKVPASATTWLNDRPEQRVPMSRLRARIAERLLESQQTNAILTTFNEVNMQPVMD
CCCCCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCHHHH
LRAKYKDKFEKEHGVKLGFMSFFVKAAVHALKKFPLVNASIDGNDIVYHGYFDIGIAVGS
HHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHCCCEECCCCCCEEEEEEEEEEEEEECC
PRGLVVPILRNADQLSLAEIEKKIAEFGQKAKDGKLSIEEMTGGTFSISNGGVFGSMLST
CCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEECCCCEEHHHHCC
PIINPPQSAILGVHATKERPVVENGQIVIRPINYLALSYDHRIIDGREAVLSLVAMKDAL
CCCCCCHHHEEEEECCCCCCCCCCCCEEEEECCEEEEECCCEEECCHHHHHHHHHHHHHH
EDPARLLLDL
CCHHHHHHCC
>Mature Secondary Structure 
AIVEVKVPQLSESVSEATMLQWKKKPGEAVAQDEILIELETDKVVLEVPAPAAGVLAQV
EEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCEEEEECCCCHHHHHHHH
LQNDGDTVVADQVIATIDTEAKAGAAEAAAGAAEVKPAAAPAAAPAAQPAAATAAASTAT
HHCCCCEEEHHHHHHEECCCCCCCCHHHHCCCHHCCCCCCCCCCCCCCCHHHHHHHHHCC
ASSGTASPAAAKLLAEKGLSAGDVAGSGRDGRVTKGDALAAGSAPKAAAPAAAPAKAAAK
CCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCHHHHCC
PALPEVKVPASATTWLNDRPEQRVPMSRLRARIAERLLESQQTNAILTTFNEVNMQPVMD
CCCCCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCHHHH
LRAKYKDKFEKEHGVKLGFMSFFVKAAVHALKKFPLVNASIDGNDIVYHGYFDIGIAVGS
HHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHCCCEECCCCCCEEEEEEEEEEEEEECC
PRGLVVPILRNADQLSLAEIEKKIAEFGQKAKDGKLSIEEMTGGTFSISNGGVFGSMLST
CCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEECCCCEEHHHHCC
PIINPPQSAILGVHATKERPVVENGQIVIRPINYLALSYDHRIIDGREAVLSLVAMKDAL
CCCCCCHHHEEEEECCCCCCCCCCCCEEEEECCEEEEECCCEEECCHHHHHHHHHHHHHH
EDPARLLLDL
CCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867378 [H]