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The map label for this gene is dusA [H]

Identifier: 161524837

GI number: 161524837

Start: 1836379

End: 1837452

Strand: Reverse

Name: dusA [H]

Synonym: Bmul_1664

Alternate gene names: 161524837

Gene position: 1837452-1836379 (Counterclockwise)

Preceding gene: 161524838

Following gene: 161524830

Centisome position: 53.28

GC content: 65.08

Gene sequence:

>1074_bases
ATGCCCGGTTGTTCCCGCGCCGCCTGTTGCCCCGATTCCATGTCCGCTTCGTCTTCGCTTCCTCCTCGCCGCGTTTCCGT
CGCGCCCATGCTCGACTGGACCGATCGTCATTGCCGCTCGTTCCATCGCACGCTGACGCGCAACACGTGGCTGTATACGG
AGATGATCACGACGGGCGCGCTGCTGTTCGGCGACGTCGCGCGGCATCTCGCGTTCACGCCGAACGAGTCGCCGGTCGCG
CTGCAGCTCGGCGGCAGCGAGCCGGACGATCTTGCGCGCGCGGCCAAGCTCGGCGAGCAGTGGGGCTACGACGAAATCAA
CCTGAACTGCGGCTGCCCGTCCGAACGCGTGCAGCGCGGTGCATTCGGCGCGTGCCTGATGAACGAGCCGCAGCTCGTCG
CCGACTGCGTGAAGGCGATGCGCGATGCGGTGTCGGTGCCCGTGACGGTCAAGCACCGGATCGGCGTCGATGCGGTCGAG
GATTACGCGTTCGTGCGCGACTTCGTCGGCACGGTGGCGGAAGCGGGGTGCTCGACGTTCATCGTGCATGCGCGCAATGC
GATCCTGAAGGGCCTGTCGCCGAAGGAGAATCGCGAGATTCCGCCGCTCAAGTACGACTATGCGTATCGGTTGAAGCGCG
AGTTCCCGTCGCTCGAGATCGTGATCAACGGCGGGATCAAGACGCTCGACGAGGTGGCGCAGCATCTCGAGCACGTCGAC
GGCGTGATGCTCGGCCGCGAGGCCTATCACAACCCGTACGTGCTCGCCGACGTCGATGCACGCTTCTACGGATCGACCGA
CGCCGCGCCGACGCGCGAAGAGGCCGAGGCGCGGCTGATCGAATACTGTGCGGCGGAACTGAAGCGCGGCACGTACCTCG
GCGCGATCGTGCGGCATGCGCTCGGTCTGTATCGCGGCGTGCCGGGCGCACGCGGCTGGCGTCGCGTGCTGTCGGACAAC
AAGAAGCTCGCGCGCGGCGATCTGGCCGTGTTCGACGAGGCGCGCGCGCATCTGAACGACGTCGAAGAAATTTTTGAAAA
AAAGGCTTTGCAAGATTCAAAAGAGTTCGTATAA

Upstream 100 bases:

>100_bases
GAGCGCGTGCTCGACGAAGCGCGGCGCGCGGTCGACGCGGAACTCGGCGACGCGAGCGCGCGTTAGCCGCGCCGGCCAGG
CGTGCGCCGGTTGCGGTAAA

Downstream 100 bases:

>100_bases
TCTTGCTTCTTCGCTGCTGAACAGCAAACGACGCAGCGGAGAACAAAGCAGTATCAGTGGTGGCTGTAGCTCAGTTGGTA
GAGTCCAGGATTGTGATTCC

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MPGCSRAACCPDSMSASSSLPPRRVSVAPMLDWTDRHCRSFHRTLTRNTWLYTEMITTGALLFGDVARHLAFTPNESPVA
LQLGGSEPDDLARAAKLGEQWGYDEINLNCGCPSERVQRGAFGACLMNEPQLVADCVKAMRDAVSVPVTVKHRIGVDAVE
DYAFVRDFVGTVAEAGCSTFIVHARNAILKGLSPKENREIPPLKYDYAYRLKREFPSLEIVINGGIKTLDEVAQHLEHVD
GVMLGREAYHNPYVLADVDARFYGSTDAAPTREEAEARLIEYCAAELKRGTYLGAIVRHALGLYRGVPGARGWRRVLSDN
KKLARGDLAVFDEARAHLNDVEEIFEKKALQDSKEFV

Sequences:

>Translated_357_residues
MPGCSRAACCPDSMSASSSLPPRRVSVAPMLDWTDRHCRSFHRTLTRNTWLYTEMITTGALLFGDVARHLAFTPNESPVA
LQLGGSEPDDLARAAKLGEQWGYDEINLNCGCPSERVQRGAFGACLMNEPQLVADCVKAMRDAVSVPVTVKHRIGVDAVE
DYAFVRDFVGTVAEAGCSTFIVHARNAILKGLSPKENREIPPLKYDYAYRLKREFPSLEIVINGGIKTLDEVAQHLEHVD
GVMLGREAYHNPYVLADVDARFYGSTDAAPTREEAEARLIEYCAAELKRGTYLGAIVRHALGLYRGVPGARGWRRVLSDN
KKLARGDLAVFDEARAHLNDVEEIFEKKALQDSKEFV
>Mature_356_residues
PGCSRAACCPDSMSASSSLPPRRVSVAPMLDWTDRHCRSFHRTLTRNTWLYTEMITTGALLFGDVARHLAFTPNESPVAL
QLGGSEPDDLARAAKLGEQWGYDEINLNCGCPSERVQRGAFGACLMNEPQLVADCVKAMRDAVSVPVTVKHRIGVDAVED
YAFVRDFVGTVAEAGCSTFIVHARNAILKGLSPKENREIPPLKYDYAYRLKREFPSLEIVINGGIKTLDEVAQHLEHVDG
VMLGREAYHNPYVLADVDARFYGSTDAAPTREEAEARLIEYCAAELKRGTYLGAIVRHALGLYRGVPGARGWRRVLSDNK
KLARGDLAVFDEARAHLNDVEEIFEKKALQDSKEFV

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=239, Percent_Identity=30.9623430962343, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI40807366, Length=250, Percent_Identity=27.6, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI145693211, Length=312, Percent_Identity=60.5769230769231, Blast_Score=383, Evalue=1e-107,
Organism=Escherichia coli, GI1788462, Length=188, Percent_Identity=31.9148936170213, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1789660, Length=240, Percent_Identity=24.1666666666667, Blast_Score=75, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI25144369, Length=212, Percent_Identity=28.7735849056604, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17507177, Length=177, Percent_Identity=27.683615819209, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323560, Length=241, Percent_Identity=26.9709543568465, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24580595, Length=296, Percent_Identity=26.6891891891892, Blast_Score=85, Evalue=7e-17,
Organism=Drosophila melanogaster, GI19920448, Length=296, Percent_Identity=26.6891891891892, Blast_Score=85, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 39513; Mature: 39382

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGCSRAACCPDSMSASSSLPPRRVSVAPMLDWTDRHCRSFHRTLTRNTWLYTEMITTGA
CCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
LLFGDVARHLAFTPNESPVALQLGGSEPDDLARAAKLGEQWGYDEINLNCGCPSERVQRG
HHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHCC
AFGACLMNEPQLVADCVKAMRDAVSVPVTVKHRIGVDAVEDYAFVRDFVGTVAEAGCSTF
CCCHHHCCCCHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCHHHH
IVHARNAILKGLSPKENREIPPLKYDYAYRLKREFPSLEIVINGGIKTLDEVAQHLEHVD
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHH
GVMLGREAYHNPYVLADVDARFYGSTDAAPTREEAEARLIEYCAAELKRGTYLGAIVRHA
HHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
LGLYRGVPGARGWRRVLSDNKKLARGDLAVFDEARAHLNDVEEIFEKKALQDSKEFV
HHHHHCCCCCHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PGCSRAACCPDSMSASSSLPPRRVSVAPMLDWTDRHCRSFHRTLTRNTWLYTEMITTGA
CCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
LLFGDVARHLAFTPNESPVALQLGGSEPDDLARAAKLGEQWGYDEINLNCGCPSERVQRG
HHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHCC
AFGACLMNEPQLVADCVKAMRDAVSVPVTVKHRIGVDAVEDYAFVRDFVGTVAEAGCSTF
CCCHHHCCCCHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCHHHH
IVHARNAILKGLSPKENREIPPLKYDYAYRLKREFPSLEIVINGGIKTLDEVAQHLEHVD
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHH
GVMLGREAYHNPYVLADVDARFYGSTDAAPTREEAEARLIEYCAAELKRGTYLGAIVRHA
HHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
LGLYRGVPGARGWRRVLSDNKKLARGDLAVFDEARAHLNDVEEIFEKKALQDSKEFV
HHHHHCCCCCHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]