Click here to switch to the map view.

The map label for this gene is dmlR [H]

Identifier: 161524666

GI number: 161524666

Start: 1644434

End: 1645375

Strand: Reverse

Name: dmlR [H]

Synonym: Bmul_1493

Alternate gene names: 161524666

Gene position: 1645375-1644434 (Counterclockwise)

Preceding gene: 161524670

Following gene: 161524665

Centisome position: 47.71

GC content: 70.91

Gene sequence:

>942_bases
ATGAAGGCCGACCCGATTCCGGCGCCCGACAAGCCGCTCGATCTGCTCGACGTCGGGCTGTTCGTGCGCGCCGCGCTGCT
CGCAAACGTGACGGCCGCCGGGCGCGAATTCGGCGTGTCGGCCGCGGTCGCGAGCGCGCGCATCGCGCAGCTCGAACGCC
AGCTCGGCGCGCGGCTGCTGCACCGCACCACGCGCCGGATCAGCCTGACGCAGGACGGCGAGATCTTCATGGCGCGCGCC
GAAGCGCTGCTCTCGGCTGCCGACGCCGCGCGCGCATCGGTCGGCCGCGGCCGCACCGAGCCTTACGGGCGGCTGAAGGT
GTCGATGTCGTCGTCGTTCGGCCGCCAGCATGTGACGCCGGTAATTCCGGCGTTCCTGCGCCGCTATCCGTCGGTATCGC
TCGACCTGCGGCTGTCCGACGAAATCGTCGATCTGGTCGACGAAGGCTACGACGTCGCGATCCGTCTCGGCGCACTGAAG
GATTCGACGCTCGTCGCGCGCAAGCTCGCGACCAACCGCCGCGTGCTGTGCTGCTCGCCCGCCTATCTCGCCGAGCGCGG
CACGCCGCGCCATCCGGCCGATCTCGCGCAGCACGAGTGCGTGATCCTCGGCGACCAGCGCGACTGGTCGTTCGCGACGC
CGCAGGGGCCGTTCACGGTGCGCGTCGGCGGCCGGCTCGTCGCGAGCAACGGCGAGGCGATCCGCGAAGCGCTGGTCGAC
GGCTTCGGGATCGCGATCAAGTCGACCTGGGACGTCGGCCCGCTGCTCGCGAGCGGCGCGCTCGTCACCGTGCTCGACGA
CTATCCGTTCGCCGAGGACATCGCCGTCTGGGCCGTCTATCCGAGCCGTGCGCACGTGCCGCTGAAGACGCTCGCGTTCA
TCGCGTTCCTCGCCGAGCATTTCGGCGATCCGCCGTACTGGGACCGCATCGACGCGCGCTAG

Upstream 100 bases:

>100_bases
AGCAGCGGCCGCGGCGCGGCCCATGAACGTCATCGTAGGCCGCGCGATCCTTAGAAAAAACCGCTATATTTCCTGAAACA
TTTTCAGGAATTTCCGAACA

Downstream 100 bases:

>100_bases
CCGCCGCGTGCGGCCGACCGGCTACAATGTCGCGTTCGCGACGCGCCGGCCCGGCAGCCAACCGGAGCGCGCGTCCGCAG
GCGGCCGCATGCCGGCCGCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MKADPIPAPDKPLDLLDVGLFVRAALLANVTAAGREFGVSAAVASARIAQLERQLGARLLHRTTRRISLTQDGEIFMARA
EALLSAADAARASVGRGRTEPYGRLKVSMSSSFGRQHVTPVIPAFLRRYPSVSLDLRLSDEIVDLVDEGYDVAIRLGALK
DSTLVARKLATNRRVLCCSPAYLAERGTPRHPADLAQHECVILGDQRDWSFATPQGPFTVRVGGRLVASNGEAIREALVD
GFGIAIKSTWDVGPLLASGALVTVLDDYPFAEDIAVWAVYPSRAHVPLKTLAFIAFLAEHFGDPPYWDRIDAR

Sequences:

>Translated_313_residues
MKADPIPAPDKPLDLLDVGLFVRAALLANVTAAGREFGVSAAVASARIAQLERQLGARLLHRTTRRISLTQDGEIFMARA
EALLSAADAARASVGRGRTEPYGRLKVSMSSSFGRQHVTPVIPAFLRRYPSVSLDLRLSDEIVDLVDEGYDVAIRLGALK
DSTLVARKLATNRRVLCCSPAYLAERGTPRHPADLAQHECVILGDQRDWSFATPQGPFTVRVGGRLVASNGEAIREALVD
GFGIAIKSTWDVGPLLASGALVTVLDDYPFAEDIAVWAVYPSRAHVPLKTLAFIAFLAEHFGDPPYWDRIDAR
>Mature_313_residues
MKADPIPAPDKPLDLLDVGLFVRAALLANVTAAGREFGVSAAVASARIAQLERQLGARLLHRTTRRISLTQDGEIFMARA
EALLSAADAARASVGRGRTEPYGRLKVSMSSSFGRQHVTPVIPAFLRRYPSVSLDLRLSDEIVDLVDEGYDVAIRLGALK
DSTLVARKLATNRRVLCCSPAYLAERGTPRHPADLAQHECVILGDQRDWSFATPQGPFTVRVGGRLVASNGEAIREALVD
GFGIAIKSTWDVGPLLASGALVTVLDDYPFAEDIAVWAVYPSRAHVPLKTLAFIAFLAEHFGDPPYWDRIDAR

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=294, Percent_Identity=39.1156462585034, Blast_Score=196, Evalue=1e-51,
Organism=Escherichia coli, GI1789440, Length=297, Percent_Identity=30.976430976431, Blast_Score=161, Evalue=6e-41,
Organism=Escherichia coli, GI1786401, Length=293, Percent_Identity=32.4232081911263, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI1787128, Length=291, Percent_Identity=28.5223367697594, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI145693193, Length=284, Percent_Identity=31.6901408450704, Blast_Score=127, Evalue=9e-31,
Organism=Escherichia coli, GI1789639, Length=288, Percent_Identity=29.1666666666667, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1787589, Length=279, Percent_Identity=25.8064516129032, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1789173, Length=277, Percent_Identity=27.4368231046931, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1786448, Length=281, Percent_Identity=28.4697508896797, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788706, Length=191, Percent_Identity=28.7958115183246, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33949; Mature: 33949

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKADPIPAPDKPLDLLDVGLFVRAALLANVTAAGREFGVSAAVASARIAQLERQLGARLL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHHHHHH
HRTTRRISLTQDGEIFMARAEALLSAADAARASVGRGRTEPYGRLKVSMSSSFGRQHVTP
HHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCHH
VIPAFLRRYPSVSLDLRLSDEIVDLVDEGYDVAIRLGALKDSTLVARKLATNRRVLCCSP
HHHHHHHHCCCCEEEEEECHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHCCCEEEEECC
AYLAERGTPRHPADLAQHECVILGDQRDWSFATPQGPFTVRVGGRLVASNGEAIREALVD
HHHHHCCCCCCCHHHCCCCEEEEECCCCCCCCCCCCCEEEEECCEEEECCCHHHHHHHHC
GFGIAIKSTWDVGPLLASGALVTVLDDYPFAEDIAVWAVYPSRAHVPLKTLAFIAFLAEH
CCCEEEECCCCCCHHHHCCCEEEEECCCCCHHCEEEEEECCCCCCCCHHHHHHHHHHHHH
FGDPPYWDRIDAR
CCCCCCCCCCCCC
>Mature Secondary Structure
MKADPIPAPDKPLDLLDVGLFVRAALLANVTAAGREFGVSAAVASARIAQLERQLGARLL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHHHHHH
HRTTRRISLTQDGEIFMARAEALLSAADAARASVGRGRTEPYGRLKVSMSSSFGRQHVTP
HHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCHH
VIPAFLRRYPSVSLDLRLSDEIVDLVDEGYDVAIRLGALKDSTLVARKLATNRRVLCCSP
HHHHHHHHCCCCEEEEEECHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHCCCEEEEECC
AYLAERGTPRHPADLAQHECVILGDQRDWSFATPQGPFTVRVGGRLVASNGEAIREALVD
HHHHHCCCCCCCHHHCCCCEEEEECCCCCCCCCCCCCEEEEECCEEEECCCHHHHHHHHC
GFGIAIKSTWDVGPLLASGALVTVLDDYPFAEDIAVWAVYPSRAHVPLKTLAFIAFLAEH
CCCEEEECCCCCCHHHHCCCEEEEECCCCCHHCEEEEEECCCCCCCCHHHHHHHHHHHHH
FGDPPYWDRIDAR
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]