Click here to switch to the map view.

The map label for this gene is psdht [H]

Identifier: 161524498

GI number: 161524498

Start: 1455013

End: 1456032

Strand: Reverse

Name: psdht [H]

Synonym: Bmul_1325

Alternate gene names: 161524498

Gene position: 1456032-1455013 (Counterclockwise)

Preceding gene: 161524499

Following gene: 161524497

Centisome position: 42.22

GC content: 68.43

Gene sequence:

>1020_bases
ATGTCGACTGAACCGCAGGGCCTCGTTCATACGACGATCGACGGCGAGCCGATTCCGACGCTCGACGAAATCGCCGCGCA
GCATTTCGCGCTGACGCCGTGGGTCGCGCGCACGCCCGTGTTCGAGCGCGCCGATCTGCCGTCGCTCGAGGGCACGCACG
TGAACTTCAAGTTCGAACTGCTGCAGGTGAGCGGCAGCTTCAAGGCGCGCGGCGCGTTCACGCACCTGCTCGCGCTCGAC
GATGCGCGCAAGAACGCGGGCGTCACCTGCGTATCGGCGGGCAACCATGCGGCGGCCGTCGCGTATGCGGCGATGCGGCT
CGGTCATGGCGCGAAGGTCGTGATGTTCCATACCGCGAGCCCGACGCGCATCGCGCAATGCAAGCAGTACCGCGCCGAAG
TCGTGCTCGCGGGCAGCGCCTCGCAAGCATTCGAAATCGTGCGGCGGATCGAGGCCGAGGAAGGCCGCTTCTTTATCCAT
CCGTTCAACGGCTATCACACGATTCTCGGCACCGCGACGCTCGGCTACGAATGGGCGACGCAGGCGCCCGACCTCGACGC
GGTGATCGTGCCGATCGGCGGCGGCGGGCTCGCGGCCGGCGCCGCGACCGCGCTGCGTCTCGCGAATCCGCACGTGCGCG
TGTACGGCGTCGAGCCCGAAGGCGCCGACGCGATGAGCCGCAGCTTCGCGGCCAATCACACGATCAAGATGAGCGCGATG
CAGACGATCGCCGATTCGCTGATGGCGCCGCACACGGAGGAATACAGCTACCAGCTCTGCCGTCGTCATATCGACCGGAT
CGTCACGGTGTCCGACGACGCGCTGCGCGCGGCGATGCTGTTCCTGTTCGCGAACCTGAAGCTCGCGGTCGAGCCGGCGT
GCGCGGCGGCGCTCGCGGCGCTGCTCGGGCCGCTGCGCGAGCCGCTGCAGGGCAAGCGCGTCGGCGTGCTGCTGTCGGGC
ACGAATACCGATCCGGCGACGCTCGGCATGCATCTCGCGCACGCGAAGCTGCAGCGCTGA

Upstream 100 bases:

>100_bases
CGCAAGTTCATCCGCAGCATGTCGGTCGACGCACACTGACCGCTCTCTCGCCCGCCGGCCCGTGTGCGCGGGCCGGTTCA
CCTCCGCCACACGCCACGCC

Downstream 100 bases:

>100_bases
CGCGCGCGAGTGACCTAGGGTTATCCCCAGTTTGTGTTGACAGCCCTGTTGATAACTCGCCGAACAAACCCGCAAGCGGT
TGAACGGGCAGCGTTTTACG

Product: pyridoxal-5'-phosphate-dependent protein subunit beta

Products: 2-oxobutanoate; NH3

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MSTEPQGLVHTTIDGEPIPTLDEIAAQHFALTPWVARTPVFERADLPSLEGTHVNFKFELLQVSGSFKARGAFTHLLALD
DARKNAGVTCVSAGNHAAAVAYAAMRLGHGAKVVMFHTASPTRIAQCKQYRAEVVLAGSASQAFEIVRRIEAEEGRFFIH
PFNGYHTILGTATLGYEWATQAPDLDAVIVPIGGGGLAAGAATALRLANPHVRVYGVEPEGADAMSRSFAANHTIKMSAM
QTIADSLMAPHTEEYSYQLCRRHIDRIVTVSDDALRAAMLFLFANLKLAVEPACAAALAALLGPLREPLQGKRVGVLLSG
TNTDPATLGMHLAHAKLQR

Sequences:

>Translated_339_residues
MSTEPQGLVHTTIDGEPIPTLDEIAAQHFALTPWVARTPVFERADLPSLEGTHVNFKFELLQVSGSFKARGAFTHLLALD
DARKNAGVTCVSAGNHAAAVAYAAMRLGHGAKVVMFHTASPTRIAQCKQYRAEVVLAGSASQAFEIVRRIEAEEGRFFIH
PFNGYHTILGTATLGYEWATQAPDLDAVIVPIGGGGLAAGAATALRLANPHVRVYGVEPEGADAMSRSFAANHTIKMSAM
QTIADSLMAPHTEEYSYQLCRRHIDRIVTVSDDALRAAMLFLFANLKLAVEPACAAALAALLGPLREPLQGKRVGVLLSG
TNTDPATLGMHLAHAKLQR
>Mature_338_residues
STEPQGLVHTTIDGEPIPTLDEIAAQHFALTPWVARTPVFERADLPSLEGTHVNFKFELLQVSGSFKARGAFTHLLALDD
ARKNAGVTCVSAGNHAAAVAYAAMRLGHGAKVVMFHTASPTRIAQCKQYRAEVVLAGSASQAFEIVRRIEAEEGRFFIHP
FNGYHTILGTATLGYEWATQAPDLDAVIVPIGGGGLAAGAATALRLANPHVRVYGVEPEGADAMSRSFAANHTIKMSAMQ
TIADSLMAPHTEEYSYQLCRRHIDRIVTVSDDALRAAMLFLFANLKLAVEPACAAALAALLGPLREPLQGKRVGVLLSGT
NTDPATLGMHLAHAKLQR

Specific function: Acts On Both Serine And Threonine, And Properly Considered As A Hydroxy Amino Acid Deaminase. [C]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI11345492, Length=309, Percent_Identity=31.0679611650485, Blast_Score=143, Evalue=2e-34,
Organism=Escherichia coli, GI1789505, Length=271, Percent_Identity=39.1143911439114, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI1790207, Length=301, Percent_Identity=31.5614617940199, Blast_Score=122, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI71991565, Length=308, Percent_Identity=35.0649350649351, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17508781, Length=314, Percent_Identity=29.6178343949045, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17537387, Length=308, Percent_Identity=32.7922077922078, Blast_Score=124, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6322631, Length=309, Percent_Identity=35.5987055016181, Blast_Score=161, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6320930, Length=271, Percent_Identity=31.7343173431734, Blast_Score=110, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6319788, Length=284, Percent_Identity=23.2394366197183, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21355833, Length=311, Percent_Identity=35.3697749196142, Blast_Score=141, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24645328, Length=219, Percent_Identity=35.1598173515982, Blast_Score=92, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: 4.3.1.19

Molecular weight: Translated: 36235; Mature: 36104

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTEPQGLVHTTIDGEPIPTLDEIAAQHFALTPWVARTPVFERADLPSLEGTHVNFKFEL
CCCCCCCCEEEECCCCCCCCHHHHHHHHEECCCCHHCCCCCCCCCCCCCCCCEEEEEEEE
LQVSGSFKARGAFTHLLALDDARKNAGVTCVSAGNHAAAVAYAAMRLGHGAKVVMFHTAS
EEECCCCCCCCCEEEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCC
PTRIAQCKQYRAEVVLAGSASQAFEIVRRIEAEEGRFFIHPFNGYHTILGTATLGYEWAT
CHHHHHHHHCCEEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCEEEEEEEEECCEEECC
QAPDLDAVIVPIGGGGLAAGAATALRLANPHVRVYGVEPEGADAMSRSFAANHTIKMSAM
CCCCCCEEEEEECCCCHHHHHHHHHEECCCCEEEEEECCCCCHHHHHHHHCCCEEEHHHH
QTIADSLMAPHTEEYSYQLCRRHIDRIVTVSDDALRAAMLFLFANLKLAVEPACAAALAA
HHHHHHHHCCCCHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHCCCEEECHHHHHHHHH
LLGPLREPLQGKRVGVLLSGTNTDPATLGMHLAHAKLQR
HHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STEPQGLVHTTIDGEPIPTLDEIAAQHFALTPWVARTPVFERADLPSLEGTHVNFKFEL
CCCCCCCEEEECCCCCCCCHHHHHHHHEECCCCHHCCCCCCCCCCCCCCCCEEEEEEEE
LQVSGSFKARGAFTHLLALDDARKNAGVTCVSAGNHAAAVAYAAMRLGHGAKVVMFHTAS
EEECCCCCCCCCEEEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCC
PTRIAQCKQYRAEVVLAGSASQAFEIVRRIEAEEGRFFIHPFNGYHTILGTATLGYEWAT
CHHHHHHHHCCEEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCEEEEEEEEECCEEECC
QAPDLDAVIVPIGGGGLAAGAATALRLANPHVRVYGVEPEGADAMSRSFAANHTIKMSAM
CCCCCCEEEEEECCCCHHHHHHHHHEECCCCEEEEEECCCCCHHHHHHHHCCCEEEHHHH
QTIADSLMAPHTEEYSYQLCRRHIDRIVTVSDDALRAAMLFLFANLKLAVEPACAAALAA
HHHHHHHHCCCCHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHCCCEEECHHHHHHHHH
LLGPLREPLQGKRVGVLLSGTNTDPATLGMHLAHAKLQR
HHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: AMP; L-Val; pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {L-Ser}} 5 {L-Ser}} 91 {L-Thr}} 70 {L-Thr}} 11 {L-Thr}} [C]

Substrates: L-threonine

Specific reaction: L-threonine = 2-oxobutanoate + NH3

General reaction: Deamination; elimination of NH alphabeta-position of amino acid [C]

Inhibitor: Ile [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12596884; 8743570 [H]