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The map label for this gene is ppsA [H]

Identifier: 161524447

GI number: 161524447

Start: 1403564

End: 1405963

Strand: Direct

Name: ppsA [H]

Synonym: Bmul_1274

Alternate gene names: 161524447

Gene position: 1403564-1405963 (Clockwise)

Preceding gene: 161524445

Following gene: 161524448

Centisome position: 40.7

GC content: 64.38

Gene sequence:

>2400_bases
ATGACTAACGCAGCAAACGTCGCAAAGGACCAGGCGTATGTAATTCCGTTCGAGCAGTTGCGGATGACCGATGTGGAAAT
CGTGGGCGGCAAGAATGCGTCGCTCGGCGAGATGATCAGCCAGCTTGCCGAGGCAGGCGTGCGCGTTCCCACCGGTTTCG
CCACGACCGCGCTCGCATTTCGCGATTTCCTCAAGCACAACGACCTCACCGACCGCATCGCGAAGCGTCTCGAGTCGCTC
GACATCGACGACGTGAAGGCGCTCGCCGAAGCCGGCGCCGAGATCCGCAAGTGGATCGTCGATGCGCCGCTGCAGCCGCG
CCTCGAGCAGGAAATCCGCGCGCAATTCGAAGTGCTGCAAAGCGGCTCGCCGGGCGAGCTGTCGTTCGCCGTGCGTTCGT
CCGCCACGGCCGAAGATCTGCCCGACGCCTCGTTCGCCGGTCAGCAGGAGTCGTATCTGAACGTCGTCGGCATCGAGGAC
GTGCTCGACCGCATGAAGCACGTGTTCGCGTCGCTGTACAACGACCGCGCGATCTCGTATCGCGTGCACAAGGGCTTTAC
GCATGCCGAAGTCGCGCTGTCGGCCGGCGTGCAGCGCATGGTCCGTTCGGACGTCGGCGCGGCCGGCGTGATGTTCACGA
TCGACACCGAGTCGGGCTTCAAGGATGCGGTGTTCATCACGTCGAGCTACGGTCTCGGCGAAACGGTCGTGCAGGGCGCC
GTGAACCCGGACGAGTTCTACGTGTTCAAGACCACGCTCGCACAGGACAAGTACCCGATCATCCGCCGCTCGATCGGCTC
GAAGCTGATCAAGATGGAATTCACGCAGCCGGGCGAGCCGGGCCGCGTGAAGACGGTCGACGTGCCGCACGAGCAGCGCA
ACCGCTACTCGATCACCGACGACGACGTGATCGAGCTCGCGAAGTACGCGGTCATCATCGAGAAGCACTACCAGCGTCCG
ATGGACATCGAGTGGGGCAAGGACGGCCGCGACGGCAAGATCTTCATCCTGCAGGCGCGTCCGGAAACGGTGAAGAGCCA
GGCAAGCGGCAAGGCCGAGCAGCGCTTCAAGCTGAAGGGCCAGTCGCAGGTGCTCGCGACCGGCCGTGCGATCGGCCAGA
AGATCGGTGCGGGCCCCGTGCGCGTGATCCAGGATCCGTCGGAAATGGAACGCGTGCAGCCGGGCGACGTGCTCGTCGCC
GACATGACCGACCCGAACTGGGAGCCGGTGATGAAGCGCGCGTCCGCGATCGTCACGAACCGTGGCGGGCGCACCTGCCA
CGCGGCGATCATCGCGCGTGAGCTCGGCGTGCCGGCCGTGGTCGGCTGCGGCGACGCGACCGACGTGCTGAAGGACGGCG
CGCTCGTCACCGTGTCGTGCGCGGAAGGCGACGAAGGCAAGATCTACGACGGGCTGCTCGAGACGGAAGTCACCGAAGTG
CAGCGCGGCGAACTGCCGGAAATTCCGGTGAAGATCATGATGAACGTCGGCAACCCGCAGCTCGCATTCGACTTCTCGCA
GCTGCCGAACGCCGGCGTCGGCCTCGCGCGTCTCGAGTTCATCATCAACAACAACATCGGCGTGCACCCGAAGGCGATCC
TCGAGTATCCGAACGTCGATCAGGATCTGAAGAAGGCCGTCGAGAGCGTCGCGCGCGGTCATGCGTCGCCGCGTGCGTTC
TACGTCGACAAGCTGACCGAAGGCGTCGCGACGATTGCCGCGGCGTTCTATCCGAAGCCCGTGATCGTGCGTCTGTCCGA
CTTCAAGTCGAACGAGTACAAGAAGCTGATCGGCGGTTCGCGCTACGAGCCGGACGAGGAAAACCCGATGCTGGGCTTCC
GCGGCGCGTCGCGCTACATCGCCGACGACTTCGCGCAGGCGTTCGAGATGGAGTGCCGTGCGCTCAAGCGCGTGCGCGAC
GAGATGGGCTTGACCAACGTCGAGATCATGGTGCCGTTCGTGCGCACCGTGAAGCAGGCGGAACGCGTCGTCGGCCTGCT
CGAGAAGTTCGGCCTGAAGCGCGGCGAGAACGGGCTGCGCCTCGTGATGATGTGCGAGGTGCCGACCAACGCGATTCTCG
CGGAAGAGTTCCTGCAGTACTTCGACGGGTTCTCGATCGGCTCGAACGACCTCACGCAGCTCACGCTCGGCCTCGATCGC
GATTCGGGCATGGAGCTGCTGGCCGTCGATTTCGACGAGCGCGATCCGGCGGTCAAGTTCCTGCTGAAGCGCGCGATCGA
TACGTGCCGCAAGATGGGCAAGTACGTCGGCATCTGCGGTCAGGGCCCGTCCGATCATCCGGATTTCGCGCAGTGGCTGA
CCGACGAAGGCATCGTGTCGATCTCGCTGAACCCGGACACGATCATCGATACCTGGCAGGCGCTCGCGAACCGGAAGTAA

Upstream 100 bases:

>100_bases
TATCTAACAGGTTGCGCAAGACGCGCCGCGCCTTTTTGCAAGCGCCCGCGTTCAAGCCCACTTGCGCAATCGAGCATTTT
TTTCACACTTAGGGGCTTGT

Downstream 100 bases:

>100_bases
CGGTCGATCGCGTCGCTTCGACGAAAGGGCGGCTCGGCCGCACTTTCGTCGAAGGCAAAATGCAAGGTTCATGCTATAAA
CACCCCGGTAGATCCGGGGT

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 799; Mature: 798

Protein sequence:

>799_residues
MTNAANVAKDQAYVIPFEQLRMTDVEIVGGKNASLGEMISQLAEAGVRVPTGFATTALAFRDFLKHNDLTDRIAKRLESL
DIDDVKALAEAGAEIRKWIVDAPLQPRLEQEIRAQFEVLQSGSPGELSFAVRSSATAEDLPDASFAGQQESYLNVVGIED
VLDRMKHVFASLYNDRAISYRVHKGFTHAEVALSAGVQRMVRSDVGAAGVMFTIDTESGFKDAVFITSSYGLGETVVQGA
VNPDEFYVFKTTLAQDKYPIIRRSIGSKLIKMEFTQPGEPGRVKTVDVPHEQRNRYSITDDDVIELAKYAVIIEKHYQRP
MDIEWGKDGRDGKIFILQARPETVKSQASGKAEQRFKLKGQSQVLATGRAIGQKIGAGPVRVIQDPSEMERVQPGDVLVA
DMTDPNWEPVMKRASAIVTNRGGRTCHAAIIARELGVPAVVGCGDATDVLKDGALVTVSCAEGDEGKIYDGLLETEVTEV
QRGELPEIPVKIMMNVGNPQLAFDFSQLPNAGVGLARLEFIINNNIGVHPKAILEYPNVDQDLKKAVESVARGHASPRAF
YVDKLTEGVATIAAAFYPKPVIVRLSDFKSNEYKKLIGGSRYEPDEENPMLGFRGASRYIADDFAQAFEMECRALKRVRD
EMGLTNVEIMVPFVRTVKQAERVVGLLEKFGLKRGENGLRLVMMCEVPTNAILAEEFLQYFDGFSIGSNDLTQLTLGLDR
DSGMELLAVDFDERDPAVKFLLKRAIDTCRKMGKYVGICGQGPSDHPDFAQWLTDEGIVSISLNPDTIIDTWQALANRK

Sequences:

>Translated_799_residues
MTNAANVAKDQAYVIPFEQLRMTDVEIVGGKNASLGEMISQLAEAGVRVPTGFATTALAFRDFLKHNDLTDRIAKRLESL
DIDDVKALAEAGAEIRKWIVDAPLQPRLEQEIRAQFEVLQSGSPGELSFAVRSSATAEDLPDASFAGQQESYLNVVGIED
VLDRMKHVFASLYNDRAISYRVHKGFTHAEVALSAGVQRMVRSDVGAAGVMFTIDTESGFKDAVFITSSYGLGETVVQGA
VNPDEFYVFKTTLAQDKYPIIRRSIGSKLIKMEFTQPGEPGRVKTVDVPHEQRNRYSITDDDVIELAKYAVIIEKHYQRP
MDIEWGKDGRDGKIFILQARPETVKSQASGKAEQRFKLKGQSQVLATGRAIGQKIGAGPVRVIQDPSEMERVQPGDVLVA
DMTDPNWEPVMKRASAIVTNRGGRTCHAAIIARELGVPAVVGCGDATDVLKDGALVTVSCAEGDEGKIYDGLLETEVTEV
QRGELPEIPVKIMMNVGNPQLAFDFSQLPNAGVGLARLEFIINNNIGVHPKAILEYPNVDQDLKKAVESVARGHASPRAF
YVDKLTEGVATIAAAFYPKPVIVRLSDFKSNEYKKLIGGSRYEPDEENPMLGFRGASRYIADDFAQAFEMECRALKRVRD
EMGLTNVEIMVPFVRTVKQAERVVGLLEKFGLKRGENGLRLVMMCEVPTNAILAEEFLQYFDGFSIGSNDLTQLTLGLDR
DSGMELLAVDFDERDPAVKFLLKRAIDTCRKMGKYVGICGQGPSDHPDFAQWLTDEGIVSISLNPDTIIDTWQALANRK
>Mature_798_residues
TNAANVAKDQAYVIPFEQLRMTDVEIVGGKNASLGEMISQLAEAGVRVPTGFATTALAFRDFLKHNDLTDRIAKRLESLD
IDDVKALAEAGAEIRKWIVDAPLQPRLEQEIRAQFEVLQSGSPGELSFAVRSSATAEDLPDASFAGQQESYLNVVGIEDV
LDRMKHVFASLYNDRAISYRVHKGFTHAEVALSAGVQRMVRSDVGAAGVMFTIDTESGFKDAVFITSSYGLGETVVQGAV
NPDEFYVFKTTLAQDKYPIIRRSIGSKLIKMEFTQPGEPGRVKTVDVPHEQRNRYSITDDDVIELAKYAVIIEKHYQRPM
DIEWGKDGRDGKIFILQARPETVKSQASGKAEQRFKLKGQSQVLATGRAIGQKIGAGPVRVIQDPSEMERVQPGDVLVAD
MTDPNWEPVMKRASAIVTNRGGRTCHAAIIARELGVPAVVGCGDATDVLKDGALVTVSCAEGDEGKIYDGLLETEVTEVQ
RGELPEIPVKIMMNVGNPQLAFDFSQLPNAGVGLARLEFIINNNIGVHPKAILEYPNVDQDLKKAVESVARGHASPRAFY
VDKLTEGVATIAAAFYPKPVIVRLSDFKSNEYKKLIGGSRYEPDEENPMLGFRGASRYIADDFAQAFEMECRALKRVRDE
MGLTNVEIMVPFVRTVKQAERVVGLLEKFGLKRGENGLRLVMMCEVPTNAILAEEFLQYFDGFSIGSNDLTQLTLGLDRD
SGMELLAVDFDERDPAVKFLLKRAIDTCRKMGKYVGICGQGPSDHPDFAQWLTDEGIVSISLNPDTIIDTWQALANRK

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=787, Percent_Identity=65.0571791613723, Blast_Score=1052, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=399, Percent_Identity=26.0651629072682, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI1788726, Length=192, Percent_Identity=32.2916666666667, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI48994992, Length=409, Percent_Identity=24.2053789731051, Blast_Score=83, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17564524, Length=237, Percent_Identity=31.6455696202532, Blast_Score=99, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 87952; Mature: 87821

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNAANVAKDQAYVIPFEQLRMTDVEIVGGKNASLGEMISQLAEAGVRVPTGFATTALAF
CCCCCCCCCCCEEEEEHHHHCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHH
RDFLKHNDLTDRIAKRLESLDIDDVKALAEAGAEIRKWIVDAPLQPRLEQEIRAQFEVLQ
HHHHHCCCHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHH
SGSPGELSFAVRSSATAEDLPDASFAGQQESYLNVVGIEDVLDRMKHVFASLYNDRAISY
CCCCCCEEEEEECCCCHHCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCEEEE
RVHKGFTHAEVALSAGVQRMVRSDVGAAGVMFTIDTESGFKDAVFITSSYGLGETVVQGA
EECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEECCCCCHHHHHCC
VNPDEFYVFKTTLAQDKYPIIRRSIGSKLIKMEFTQPGEPGRVKTVDVPHEQRNRYSITD
CCCCCEEEEEEECCCCCCHHHHHHHCCCEEEEECCCCCCCCCEEEEECCHHHCCCCCCCC
DDVIELAKYAVIIEKHYQRPMDIEWGKDGRDGKIFILQARPETVKSQASGKAEQRFKLKG
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHCCCHHHHEEECC
QSQVLATGRAIGQKIGAGPVRVIQDPSEMERVQPGDVLVADMTDPNWEPVMKRASAIVTN
CCHHHHHHHHHHHHCCCCCEEEECCHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCC
RGGRTCHAAIIARELGVPAVVGCGDATDVLKDGALVTVSCAEGDEGKIYDGLLETEVTEV
CCCCCHHHHHHHHHCCCCEEEECCCHHHHHCCCCEEEEEECCCCCCCEEHHHHHHHHHHH
QRGELPEIPVKIMMNVGNPQLAFDFSQLPNAGVGLARLEFIINNNIGVHPKAILEYPNVD
HCCCCCCCCEEEEEECCCCCEEEEHHHCCCCCCCHHEEEEEECCCCCCCCHHHCCCCCCC
QDLKKAVESVARGHASPRAFYVDKLTEGVATIAAAFYPKPVIVRLSDFKSNEYKKLIGGS
HHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCC
RYEPDEENPMLGFRGASRYIADDFAQAFEMECRALKRVRDEMGLTNVEIMVPFVRTVKQA
CCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHH
ERVVGLLEKFGLKRGENGLRLVMMCEVPTNAILAEEFLQYFDGFSIGSNDLTQLTLGLDR
HHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCC
DSGMELLAVDFDERDPAVKFLLKRAIDTCRKMGKYVGICGQGPSDHPDFAQWLTDEGIVS
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHCCCCEEE
ISLNPDTIIDTWQALANRK
EECCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
TNAANVAKDQAYVIPFEQLRMTDVEIVGGKNASLGEMISQLAEAGVRVPTGFATTALAF
CCCCCCCCCCEEEEEHHHHCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHH
RDFLKHNDLTDRIAKRLESLDIDDVKALAEAGAEIRKWIVDAPLQPRLEQEIRAQFEVLQ
HHHHHCCCHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHH
SGSPGELSFAVRSSATAEDLPDASFAGQQESYLNVVGIEDVLDRMKHVFASLYNDRAISY
CCCCCCEEEEEECCCCHHCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCEEEE
RVHKGFTHAEVALSAGVQRMVRSDVGAAGVMFTIDTESGFKDAVFITSSYGLGETVVQGA
EECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEECCCCCHHHHHCC
VNPDEFYVFKTTLAQDKYPIIRRSIGSKLIKMEFTQPGEPGRVKTVDVPHEQRNRYSITD
CCCCCEEEEEEECCCCCCHHHHHHHCCCEEEEECCCCCCCCCEEEEECCHHHCCCCCCCC
DDVIELAKYAVIIEKHYQRPMDIEWGKDGRDGKIFILQARPETVKSQASGKAEQRFKLKG
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHCCCHHHHEEECC
QSQVLATGRAIGQKIGAGPVRVIQDPSEMERVQPGDVLVADMTDPNWEPVMKRASAIVTN
CCHHHHHHHHHHHHCCCCCEEEECCHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCC
RGGRTCHAAIIARELGVPAVVGCGDATDVLKDGALVTVSCAEGDEGKIYDGLLETEVTEV
CCCCCHHHHHHHHHCCCCEEEECCCHHHHHCCCCEEEEEECCCCCCCEEHHHHHHHHHHH
QRGELPEIPVKIMMNVGNPQLAFDFSQLPNAGVGLARLEFIINNNIGVHPKAILEYPNVD
HCCCCCCCCEEEEEECCCCCEEEEHHHCCCCCCCHHEEEEEECCCCCCCCHHHCCCCCCC
QDLKKAVESVARGHASPRAFYVDKLTEGVATIAAAFYPKPVIVRLSDFKSNEYKKLIGGS
HHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCC
RYEPDEENPMLGFRGASRYIADDFAQAFEMECRALKRVRDEMGLTNVEIMVPFVRTVKQA
CCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHH
ERVVGLLEKFGLKRGENGLRLVMMCEVPTNAILAEEFLQYFDGFSIGSNDLTQLTLGLDR
HHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCC
DSGMELLAVDFDERDPAVKFLLKRAIDTCRKMGKYVGICGQGPSDHPDFAQWLTDEGIVS
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHCCCCEEE
ISLNPDTIIDTWQALANRK
EECCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]