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The map label for this gene is cls [H]

Identifier: 161524416

GI number: 161524416

Start: 1365793

End: 1367232

Strand: Reverse

Name: cls [H]

Synonym: Bmul_1243

Alternate gene names: 161524416

Gene position: 1367232-1365793 (Counterclockwise)

Preceding gene: 161524417

Following gene: 161524414

Centisome position: 39.65

GC content: 68.12

Gene sequence:

>1440_bases
ATGACCCTCGACTGGCTACACCTCGGCACACTGATCCTGGCCATCCACGTGCTCGGGATCGTCGCGGCCTTCCACGCGAT
CCTGAACACGCGGACGTCGCAAGGCGCCATCGCATGGGCGGTCTCGCTCGCCGCGATGCCGTACCTCACGCTGATCCCCT
ATCTGTTTCTCGGCCGCAGCAAGTTCTCCGGCTACGTCGACGCGCGCCGCCACGAAACCGAAGCGTTGCGCACGCACACA
CCGCGCGCGCCGTGGCTCGACGCGAACGCGAGCGACCGGCCGGCGGCCGATGCGCTCGGCCAGGCCGCAGTGCTCGCGCT
CAGGCGGCTCGGCGGCATGCCGTTCGTCGGCGGCAATTCGGTGCGCACGCTCGTGAACGGCGACGCGACCTTCGCGGCGA
TTCTCTCGGCGATCGACGCCGCGCGCGATTACGTGATCGTCCAGTTCTTCATCGTCCGCGACGATGCGCTCGGCCAGATG
CTGCGCGACGCGCTGCTCGCGCGCGCGGCGGCCGGCGTGCGCTGCTATCTGCTGTACGACAGCATCGGCAGCTTCGATCT
GCCGCGCAGCTACGTCGACGCGCTGCGGCGCGGCGGCGTCGAAGTCCATCCGTTCGCGACGCATCGCAAGTTCGTCAACC
GCTTCCAGCTCAACTTCCGCAATCACCGCAAGATCGTCGTCGTCGACGGCACTCGCGCGTTCGTCGGCGGACACAACGTC
GGCGTCGAGTATCTCGGCGGCAACCCGCGCCTGTCGCCGTGGCGCGACACGCACATCGAGATACGCGGGCCGGCCGTCGC
GAGCATCCAATACGTGTTCGCCGAAGACTGGCACTGGGCGACACAGACGCTGCCGTCGCTCGCCGCGCCGCCGGCCGCGC
TGCCGGACGACGACATGCACTGTCTCGCGGTGCCGATGGGGCCGGCCGACAAGCAGGAAACCGGCTCGCTGTTCTTCGTC
GAGGCGATCAACGCGGCGCGCGAGCGCGTCTGGATCACCACGCCGTACCTCGTACCCGACGAGGCCGTCGTCGCGGCGCT
TAAGCTCGCGGTGATGCGCGGCGTCGACGTGCGCATCCTGATCCCGAGCCGCCGCGATCACTACGTCGTGTTCGAGGCGT
CGAAGCTGTACGCGCGCGACCTCGTCGATGCAGGCGTCAAGGTGTTCCGCTACCGGCCGGGATTCCTGCATCAGAAGGTC
GTGCTGATCGACCGCGTCGCCGCGGCGATCGGCAGCGCGAATCTCGATAACCGCTCGTTCCGGCTGAACTTCGAGATCAT
GGTGCTGACGATCGATCGCGCGTTCGCCGATGAAGTGGCCGCGATGCTCGCCGCGGACTTCGCGCAGTCGTATGAGGTCG
ATACGAACGAGTACCGGCAGTCGCCCGCATGGCGGCGCATCGCGATGCACGTCGCGCGGCTGTTCGCGCCGATCCTGTAG

Upstream 100 bases:

>100_bases
GCGATGCAGGCGGCGCGGCAACCCGCTGCGCCGCCCCCTTGGGTAAGATGCGCGTACGCGGCCGCCGCGCCGCACGACTT
CAGGAGTCCATCACGCGCCC

Downstream 100 bases:

>100_bases
CCGCCGCGGCCCGCGTGCCCGATGCGGGCGCGCACCGCGCGTCAAAGCAGCTTTTCGATATCCGCGGCGATGTCCTCGGG
CTTTGTCGACGGCGCGTAGC

Product: cardiolipin synthetase

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MTLDWLHLGTLILAIHVLGIVAAFHAILNTRTSQGAIAWAVSLAAMPYLTLIPYLFLGRSKFSGYVDARRHETEALRTHT
PRAPWLDANASDRPAADALGQAAVLALRRLGGMPFVGGNSVRTLVNGDATFAAILSAIDAARDYVIVQFFIVRDDALGQM
LRDALLARAAAGVRCYLLYDSIGSFDLPRSYVDALRRGGVEVHPFATHRKFVNRFQLNFRNHRKIVVVDGTRAFVGGHNV
GVEYLGGNPRLSPWRDTHIEIRGPAVASIQYVFAEDWHWATQTLPSLAAPPAALPDDDMHCLAVPMGPADKQETGSLFFV
EAINAARERVWITTPYLVPDEAVVAALKLAVMRGVDVRILIPSRRDHYVVFEASKLYARDLVDAGVKVFRYRPGFLHQKV
VLIDRVAAAIGSANLDNRSFRLNFEIMVLTIDRAFADEVAAMLAADFAQSYEVDTNEYRQSPAWRRIAMHVARLFAPIL

Sequences:

>Translated_479_residues
MTLDWLHLGTLILAIHVLGIVAAFHAILNTRTSQGAIAWAVSLAAMPYLTLIPYLFLGRSKFSGYVDARRHETEALRTHT
PRAPWLDANASDRPAADALGQAAVLALRRLGGMPFVGGNSVRTLVNGDATFAAILSAIDAARDYVIVQFFIVRDDALGQM
LRDALLARAAAGVRCYLLYDSIGSFDLPRSYVDALRRGGVEVHPFATHRKFVNRFQLNFRNHRKIVVVDGTRAFVGGHNV
GVEYLGGNPRLSPWRDTHIEIRGPAVASIQYVFAEDWHWATQTLPSLAAPPAALPDDDMHCLAVPMGPADKQETGSLFFV
EAINAARERVWITTPYLVPDEAVVAALKLAVMRGVDVRILIPSRRDHYVVFEASKLYARDLVDAGVKVFRYRPGFLHQKV
VLIDRVAAAIGSANLDNRSFRLNFEIMVLTIDRAFADEVAAMLAADFAQSYEVDTNEYRQSPAWRRIAMHVARLFAPIL
>Mature_478_residues
TLDWLHLGTLILAIHVLGIVAAFHAILNTRTSQGAIAWAVSLAAMPYLTLIPYLFLGRSKFSGYVDARRHETEALRTHTP
RAPWLDANASDRPAADALGQAAVLALRRLGGMPFVGGNSVRTLVNGDATFAAILSAIDAARDYVIVQFFIVRDDALGQML
RDALLARAAAGVRCYLLYDSIGSFDLPRSYVDALRRGGVEVHPFATHRKFVNRFQLNFRNHRKIVVVDGTRAFVGGHNVG
VEYLGGNPRLSPWRDTHIEIRGPAVASIQYVFAEDWHWATQTLPSLAAPPAALPDDDMHCLAVPMGPADKQETGSLFFVE
AINAARERVWITTPYLVPDEAVVAALKLAVMRGVDVRILIPSRRDHYVVFEASKLYARDLVDAGVKVFRYRPGFLHQKVV
LIDRVAAAIGSANLDNRSFRLNFEIMVLTIDRAFADEVAAMLAADFAQSYEVDTNEYRQSPAWRRIAMHVARLFAPIL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=471, Percent_Identity=28.0254777070064, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1787007, Length=351, Percent_Identity=31.6239316239316, Blast_Score=135, Evalue=8e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 53007; Mature: 52875

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLDWLHLGTLILAIHVLGIVAAFHAILNTRTSQGAIAWAVSLAAMPYLTLIPYLFLGRS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
KFSGYVDARRHETEALRTHTPRAPWLDANASDRPAADALGQAAVLALRRLGGMPFVGGNS
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEECCCC
VRTLVNGDATFAAILSAIDAARDYVIVQFFIVRDDALGQMLRDALLARAAAGVRCYLLYD
EEEEECCCHHHHHHHHHHHHHCCEEEEEEEEEECHHHHHHHHHHHHHHHHCCEEEEEEEE
SIGSFDLPRSYVDALRRGGVEVHPFATHRKFVNRFQLNFRNHRKIVVVDGTRAFVGGHNV
CCCCCCCCHHHHHHHHHCCCEEEECHHHHHHHHHHHCCCCCCCEEEEEECCEEEECCCCC
GVEYLGGNPRLSPWRDTHIEIRGPAVASIQYVFAEDWHWATQTLPSLAAPPAALPDDDMH
CEEEECCCCCCCCCCCCEEEEECCCEEEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCE
CLAVPMGPADKQETGSLFFVEAINAARERVWITTPYLVPDEAVVAALKLAVMRGVDVRIL
EEEEECCCCCCCCCCCEEEEEEHHHHHCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEE
IPSRRDHYVVFEASKLYARDLVDAGVKVFRYRPGFLHQKVVLIDRVAAAIGSANLDNRSF
ECCCCCCEEEEECHHHHHHHHHHCCHHEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEE
RLNFEIMVLTIDRAFADEVAAMLAADFAQSYEVDTNEYRQSPAWRRIAMHVARLFAPIL
EEEEEEEEEEECHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLDWLHLGTLILAIHVLGIVAAFHAILNTRTSQGAIAWAVSLAAMPYLTLIPYLFLGRS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
KFSGYVDARRHETEALRTHTPRAPWLDANASDRPAADALGQAAVLALRRLGGMPFVGGNS
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEECCCC
VRTLVNGDATFAAILSAIDAARDYVIVQFFIVRDDALGQMLRDALLARAAAGVRCYLLYD
EEEEECCCHHHHHHHHHHHHHCCEEEEEEEEEECHHHHHHHHHHHHHHHHCCEEEEEEEE
SIGSFDLPRSYVDALRRGGVEVHPFATHRKFVNRFQLNFRNHRKIVVVDGTRAFVGGHNV
CCCCCCCCHHHHHHHHHCCCEEEECHHHHHHHHHHHCCCCCCCEEEEEECCEEEECCCCC
GVEYLGGNPRLSPWRDTHIEIRGPAVASIQYVFAEDWHWATQTLPSLAAPPAALPDDDMH
CEEEECCCCCCCCCCCCEEEEECCCEEEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCE
CLAVPMGPADKQETGSLFFVEAINAARERVWITTPYLVPDEAVVAALKLAVMRGVDVRIL
EEEEECCCCCCCCCCCEEEEEEHHHHHCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEE
IPSRRDHYVVFEASKLYARDLVDAGVKVFRYRPGFLHQKVVLIDRVAAAIGSANLDNRSF
ECCCCCCEEEEECHHHHHHHHHHCCHHEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEE
RLNFEIMVLTIDRAFADEVAAMLAADFAQSYEVDTNEYRQSPAWRRIAMHVARLFAPIL
EEEEEEEEEEECHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA