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The map label for this gene is trmJ [H]

Identifier: 161524370

GI number: 161524370

Start: 1314183

End: 1315052

Strand: Direct

Name: trmJ [H]

Synonym: Bmul_1197

Alternate gene names: 161524370

Gene position: 1314183-1315052 (Clockwise)

Preceding gene: 161524359

Following gene: 161524371

Centisome position: 38.11

GC content: 67.82

Gene sequence:

>870_bases
ATGGCGATACCATACGGCCATTCTGACGAATCGAGTCGTATGGTAGTGGAAACCTTGCAGAACACCGCTGTGCCGCCCGC
GTCCGGTGCGGCGCCGTCCCGGTCGCCGTCGGGCGGCTTCACGTCGACACGCTTCGTGCTGGTCGAACCGAGTCATCCCG
GCAACGTCGGCGCGGCCGCGCGCGCCTTGAAGACGATGGGCTTTTCGCGCCTCGTGCTCGTCGCGCCGCGCGTGCCGCAC
GTGCAAAGCGACCCGGAGGCCGTCGCGATGGCGAGCGGCGCCGACGACGTGCTCGCGTCCGCGCATGTCGTATCGACGCT
CGGCGAGGCGCTGTCCGGCGTGCACTGGTCCATCGCGCTGACCGCGCGCACGCGTGAATACGGGCCGCCGCGCCTCGCGC
CGCGCGCGGCTGCTGCCCATGCATGCACGCAGGTCGAAAGCGGCGACATCGCGCTCGTGTTCGGCAACGAGCGCACCGGG
CTCGCGAACGAGCATGTCGAGCAGTGCAGCGCGCTCGCGCATATTCCGGCGAATCCCGCCTATAGCTCGCTCAACCTCGC
GCAGGCGGTGCAGGTGCTCGCTTACGAGCTGCGTGTCGCGTTTCTCGAGCAGACGAGCGAGCCGGCGCAACAGCCCGCGG
CCGATACGGGGACGCTCGCGCAAAGCGACGAGATCGAACGGATGTATCTGCATCTGGAAAATGCGCTGATCGCGCTCGAT
TTCCTCGATCCGCGCAATCCGAAGAAGCTGATGCCGCGTCTGCGGCGCCTGTTTGCGCGCACGGGCCTCGAGCGCGAGGA
AGTGAACATCCTGCGCGGCATCGCGAAGCACATTCTGCTGAAGTCGCGCACGCCCGACGGCGGCGCATAA

Upstream 100 bases:

>100_bases
CGCGTAACGGCATGATTTTACCCGATTTTCGCCGGTTTTATGAGAGCGGAATCCGGGCAAACCCCCACTGGCCGTTCGGC
CGAGTGGGTTTGCCGCGCCG

Downstream 100 bases:

>100_bases
CCGCGTCGGCGCCGCCGGCGCAAATACCCGCGCCGCCGGCGGGGAGAACGCGCGGCGTCGTGCGGGCGCGCGCGACTGGC
CCTACAATCGCCGGACGTCA

Product: RNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MAIPYGHSDESSRMVVETLQNTAVPPASGAAPSRSPSGGFTSTRFVLVEPSHPGNVGAAARALKTMGFSRLVLVAPRVPH
VQSDPEAVAMASGADDVLASAHVVSTLGEALSGVHWSIALTARTREYGPPRLAPRAAAAHACTQVESGDIALVFGNERTG
LANEHVEQCSALAHIPANPAYSSLNLAQAVQVLAYELRVAFLEQTSEPAQQPAADTGTLAQSDEIERMYLHLENALIALD
FLDPRNPKKLMPRLRRLFARTGLEREEVNILRGIAKHILLKSRTPDGGA

Sequences:

>Translated_289_residues
MAIPYGHSDESSRMVVETLQNTAVPPASGAAPSRSPSGGFTSTRFVLVEPSHPGNVGAAARALKTMGFSRLVLVAPRVPH
VQSDPEAVAMASGADDVLASAHVVSTLGEALSGVHWSIALTARTREYGPPRLAPRAAAAHACTQVESGDIALVFGNERTG
LANEHVEQCSALAHIPANPAYSSLNLAQAVQVLAYELRVAFLEQTSEPAQQPAADTGTLAQSDEIERMYLHLENALIALD
FLDPRNPKKLMPRLRRLFARTGLEREEVNILRGIAKHILLKSRTPDGGA
>Mature_288_residues
AIPYGHSDESSRMVVETLQNTAVPPASGAAPSRSPSGGFTSTRFVLVEPSHPGNVGAAARALKTMGFSRLVLVAPRVPHV
QSDPEAVAMASGADDVLASAHVVSTLGEALSGVHWSIALTARTREYGPPRLAPRAAAAHACTQVESGDIALVFGNERTGL
ANEHVEQCSALAHIPANPAYSSLNLAQAVQVLAYELRVAFLEQTSEPAQQPAADTGTLAQSDEIERMYLHLENALIALDF
LDPRNPKKLMPRLRRLFARTGLEREEVNILRGIAKHILLKSRTPDGGA

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=239, Percent_Identity=45.6066945606695, Blast_Score=200, Evalue=7e-53,
Organism=Escherichia coli, GI1790865, Length=187, Percent_Identity=36.8983957219251, Blast_Score=91, Evalue=9e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 30726; Mature: 30595

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIPYGHSDESSRMVVETLQNTAVPPASGAAPSRSPSGGFTSTRFVLVEPSHPGNVGAAA
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHH
RALKTMGFSRLVLVAPRVPHVQSDPEAVAMASGADDVLASAHVVSTLGEALSGVHWSIAL
HHHHHCCCCEEEEECCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEE
TARTREYGPPRLAPRAAAAHACTQVESGDIALVFGNERTGLANEHVEQCSALAHIPANPA
EEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCC
YSSLNLAQAVQVLAYELRVAFLEQTSEPAQQPAADTGTLAQSDEIERMYLHLENALIALD
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCHHHHHHHHHHHCCEEEEE
FLDPRNPKKLMPRLRRLFARTGLEREEVNILRGIAKHILLKSRTPDGGA
CCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AIPYGHSDESSRMVVETLQNTAVPPASGAAPSRSPSGGFTSTRFVLVEPSHPGNVGAAA
CCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHH
RALKTMGFSRLVLVAPRVPHVQSDPEAVAMASGADDVLASAHVVSTLGEALSGVHWSIAL
HHHHHCCCCEEEEECCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEE
TARTREYGPPRLAPRAAAAHACTQVESGDIALVFGNERTGLANEHVEQCSALAHIPANPA
EEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCC
YSSLNLAQAVQVLAYELRVAFLEQTSEPAQQPAADTGTLAQSDEIERMYLHLENALIALD
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCHHHHHHHHHHHCCEEEEE
FLDPRNPKKLMPRLRRLFARTGLEREEVNILRGIAKHILLKSRTPDGGA
CCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA