Click here to switch to the map view.

The map label for this gene is xthA [H]

Identifier: 161524301

GI number: 161524301

Start: 1237503

End: 1238321

Strand: Reverse

Name: xthA [H]

Synonym: Bmul_1126

Alternate gene names: 161524301

Gene position: 1238321-1237503 (Counterclockwise)

Preceding gene: 161524302

Following gene: 161524296

Centisome position: 35.91

GC content: 65.32

Gene sequence:

>819_bases
ATGGCGGCCTTTGCATTGATCCGCACGCGCGCTTCCCCGTTCATGAAAATTGCCACCTGGAACGTCAACTCGCTCAACGT
CCGCAAGCAGCACGTGCTCGACTGGCTCGCGCAAAGCGCCGTCGACGTACTGTGCCTGCAGGAACTCAAACTGCCGGACG
AGAAATTTCCGCGCGCCGATCTCGAGGCGGCCGGCTATCGCAGCTGGTTCGCCGGCCAGAAGACCTACAACGGCGTCGCG
ATTCTCGCGCGTGACACGCTGCCGTTCGACGAATCGGACGTCGTGCGAAACATTCCGGGCTTCGAGGACACGCAGCAGCG
CGTGATCGCCGTCACGATCGACGGCGTGCGGATCGTGTCCGCCTACTTCCCGAACGGGCAGGCCGTCGGCTCCGACAAGT
TCCTGTACAAGATGCAGTGGCTCGACGCGCTGCATGCGTGGCTGCGCACCGAGATGCAGCGCTACCCGAAGCTCGCGCTG
CTCGGCGACTACAACATCGCGCCCGAAGATCGCGACGTCCACGATCCGGCGAAATGGGAAGGCGAGAACCTCGTGTCGCC
GCAGGAGCGCGCGCACTTCGCGAAGCTGATCGAGCTCGGCTTCGTCGATGCGTTCCGCCGCTTCGAGCAGCCCGAGAAGA
CCTTCACGTGGTGGGACTACCGGATGTTCGCGTTCCGCCGCAACGCCGGGCTGCGCATCGACCACATCCTGCTGTCGCCG
GCGCTCGCGGGCAGTTGCACGTCGTGCGAAGTGGATCGCGTGCCGCGCACGTGGGAGCAGCCGTCCGACCATACGCCGGT
CGTCGCGGTCGTCGGCTGA

Upstream 100 bases:

>100_bases
AAATTATAGGGTGGTTAAGCACTTACGTCAACACTTTTGTCATATTCGTCCTCAATGCATCGCGGCGCTTCGCGCGTTCC
CGCCCCGTGCTCGCGGTAAG

Downstream 100 bases:

>100_bases
CGCCGCCGCCTTGCCGCTTCTGCCCTGCTCCTGCCCCGCGTGCGCTCAGCCGCGCGCGGGCGCGGGCCCGAGATGGGTCC
ACAGAAAGCCGAATTCGGCC

Product: exodeoxyribonuclease III Xth

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MAAFALIRTRASPFMKIATWNVNSLNVRKQHVLDWLAQSAVDVLCLQELKLPDEKFPRADLEAAGYRSWFAGQKTYNGVA
ILARDTLPFDESDVVRNIPGFEDTQQRVIAVTIDGVRIVSAYFPNGQAVGSDKFLYKMQWLDALHAWLRTEMQRYPKLAL
LGDYNIAPEDRDVHDPAKWEGENLVSPQERAHFAKLIELGFVDAFRRFEQPEKTFTWWDYRMFAFRRNAGLRIDHILLSP
ALAGSCTSCEVDRVPRTWEQPSDHTPVVAVVG

Sequences:

>Translated_272_residues
MAAFALIRTRASPFMKIATWNVNSLNVRKQHVLDWLAQSAVDVLCLQELKLPDEKFPRADLEAAGYRSWFAGQKTYNGVA
ILARDTLPFDESDVVRNIPGFEDTQQRVIAVTIDGVRIVSAYFPNGQAVGSDKFLYKMQWLDALHAWLRTEMQRYPKLAL
LGDYNIAPEDRDVHDPAKWEGENLVSPQERAHFAKLIELGFVDAFRRFEQPEKTFTWWDYRMFAFRRNAGLRIDHILLSP
ALAGSCTSCEVDRVPRTWEQPSDHTPVVAVVG
>Mature_271_residues
AAFALIRTRASPFMKIATWNVNSLNVRKQHVLDWLAQSAVDVLCLQELKLPDEKFPRADLEAAGYRSWFAGQKTYNGVAI
LARDTLPFDESDVVRNIPGFEDTQQRVIAVTIDGVRIVSAYFPNGQAVGSDKFLYKMQWLDALHAWLRTEMQRYPKLALL
GDYNIAPEDRDVHDPAKWEGENLVSPQERAHFAKLIELGFVDAFRRFEQPEKTFTWWDYRMFAFRRNAGLRIDHILLSPA
LAGSCTSCEVDRVPRTWEQPSDHTPVVAVVG

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=261, Percent_Identity=32.183908045977, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI18375503, Length=261, Percent_Identity=32.183908045977, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI18375501, Length=261, Percent_Identity=32.183908045977, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI1788046, Length=269, Percent_Identity=34.9442379182156, Blast_Score=148, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI71989536, Length=265, Percent_Identity=26.0377358490566, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI221330655, Length=258, Percent_Identity=26.7441860465116, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI17136678, Length=258, Percent_Identity=26.7441860465116, Blast_Score=78, Evalue=6e-15,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 31098; Mature: 30967

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAFALIRTRASPFMKIATWNVNSLNVRKQHVLDWLAQSAVDVLCLQELKLPDEKFPRAD
CCHHHEEHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCC
LEAAGYRSWFAGQKTYNGVAILARDTLPFDESDVVRNIPGFEDTQQRVIAVTIDGVRIVS
CCCCCCHHHHCCCCCCCCEEEEEECCCCCCCCHHHHCCCCCCCCCCEEEEEEECCEEEEE
AYFPNGQAVGSDKFLYKMQWLDALHAWLRTEMQRYPKLALLGDYNIAPEDRDVHDPAKWE
EECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCC
GENLVSPQERAHFAKLIELGFVDAFRRFEQPEKTFTWWDYRMFAFRRNAGLRIDHILLSP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHEEEEEEECCCCCEEEHEEECH
ALAGSCTSCEVDRVPRTWEQPSDHTPVVAVVG
HHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEC
>Mature Secondary Structure 
AAFALIRTRASPFMKIATWNVNSLNVRKQHVLDWLAQSAVDVLCLQELKLPDEKFPRAD
CHHHEEHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCC
LEAAGYRSWFAGQKTYNGVAILARDTLPFDESDVVRNIPGFEDTQQRVIAVTIDGVRIVS
CCCCCCHHHHCCCCCCCCEEEEEECCCCCCCCHHHHCCCCCCCCCCEEEEEEECCEEEEE
AYFPNGQAVGSDKFLYKMQWLDALHAWLRTEMQRYPKLALLGDYNIAPEDRDVHDPAKWE
EECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCC
GENLVSPQERAHFAKLIELGFVDAFRRFEQPEKTFTWWDYRMFAFRRNAGLRIDHILLSP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHEEEEEEECCCCCEEEHEEECH
ALAGSCTSCEVDRVPRTWEQPSDHTPVVAVVG
HHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]