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The map label for this gene is shiA [H]

Identifier: 161524288

GI number: 161524288

Start: 1218676

End: 1220082

Strand: Direct

Name: shiA [H]

Synonym: Bmul_1113

Alternate gene names: 161524288

Gene position: 1218676-1220082 (Clockwise)

Preceding gene: 161524287

Following gene: 161524291

Centisome position: 35.34

GC content: 68.73

Gene sequence:

>1407_bases
ATGAGCGGTCCGCACACCCAACCCGCATTGCCCGCGAAGCCCGGCCGCGCCGCGTTCGCGGCGTTCGTCGGCACGACGAT
CGAGTGGTACGACTTCTATATCTACGGCACGGCCGCCGCGCTCGTGTTCGGCAAGGTGTTCTTTTCGCGCGACATGGACG
CGGGCGTCGCGACACTGCTCGCGTTCGTCACGTTCTGGGCCGGCTTCGCCGCGCGGCCGCTCGGCGGCATCGTGTTCGGC
CATCTCGGCGACCGCGTCGGCCGCAAGACCGCGCTCGTCATCACGCTCGTGATGATGGGGCTCGCGACGACCGGCATCGG
CCTGCTGCCGACCTACGCGCAGATCGGCGTATGGGCGCCGGCGGGCCTCGTCGTGCTGCGCGTGCTGCAGGGCATCGCGG
TCGGCGGCGAGTGGGGCGGCGCGGTGCTGATCGCGAGCGAGAACGCGCCGAAGCACAAGAGCATCCTCTACGCGGCATTC
GCCCAGCAGGGCTCGCCGACCGGCAACCTGCTCGCGACGGGCGCGTTCTTCGCGCTGAGCGCGATGCCGACGCCCGATTT
CCTGATGTGGGGCTGGCGCATTCCGTTCCTGCTGTCGGCGGTACTCGTGATCGTCGGGATGGTGATCCGGCTGAAGCTCG
AGGAGTCGGCCGACATGCAGCGCGTACTCGCACGCAAGCGCACCGTGAAGCTGCCGCTGCGCGAGGTCGTGCGCGATCAC
TGGGTCGTCGTGCTGCTTGCGGCCGGCACGCTGCCGGTGATCAACGTCACGTATTTCCGCAGCACGTTCGCGCTGTCGTG
GGCGACGAAGGAACTCGGCTACGCGCAGGGCACCTTCCTCGGCATCCTGTCGATGTCGCTCGTCGTGCAGTTCCTGATGC
AGCCGGTCGGCGCGTGGTTCGTGTCGAAGGTCGACATGCGCCGCGCGATGTGCTGGATCCTGATTCCGGAAATCGTGCTG
ATGCCGCTGATGTTCCATGCGGTCGGCACCGGTTCGTACTGGGTCGCGGTCGCGGGGATGTGCATCTCGACGATTCCGTC
CGCGATGTTCTACGGGGCGGTGGGCGGGGTGCTCGCGCGCGTGTTTCCGGCCAATGTCCGCTATACGGGGCGGTCGCTCG
CGTATCAGTTGAGTGCGCTCGTCGTCGGCGGCGGCACGCCGGTGCTCGCACAGGCGATCCTGAATTCGACCGGCAGCATC
GTCGGCGTCGCGATCGCGTCGGGCATCTACGCGCTCGTGTCGCTCGTTTGCATGATCGCGCTGCTCAATCGCACCGGCCA
TCGCGCGGACGCGTTGTCGAGCGCCGAGCGCAGCGATGCGGCCGAATGGGCGGCCGAGCCGGCCGGCGACGCGGCTGCCC
CGCGCGACGACGAGCGCAGGCTGAAGCCGGCCGGGGAAACCGCTTGA

Upstream 100 bases:

>100_bases
GCGAGCCGCCCGGCTGACGAACTGCCGCCGGCCGGCGGCAATCCCGGCCGCACGGACGGCGCGCCGCGTCGCGCCGCCGA
CAACAAACATGGAGACATCG

Downstream 100 bases:

>100_bases
TCGCGTGCGGCACGGCAGGGCCGCTGCACGCTGCGCGGTATGCGCCGCGGTACGCGCTGCCGCACATGCGGCCGCGCCAA
TGAAAAACGGCACCGTGCAA

Product: major facilitator transporter

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MSGPHTQPALPAKPGRAAFAAFVGTTIEWYDFYIYGTAAALVFGKVFFSRDMDAGVATLLAFVTFWAGFAARPLGGIVFG
HLGDRVGRKTALVITLVMMGLATTGIGLLPTYAQIGVWAPAGLVVLRVLQGIAVGGEWGGAVLIASENAPKHKSILYAAF
AQQGSPTGNLLATGAFFALSAMPTPDFLMWGWRIPFLLSAVLVIVGMVIRLKLEESADMQRVLARKRTVKLPLREVVRDH
WVVVLLAAGTLPVINVTYFRSTFALSWATKELGYAQGTFLGILSMSLVVQFLMQPVGAWFVSKVDMRRAMCWILIPEIVL
MPLMFHAVGTGSYWVAVAGMCISTIPSAMFYGAVGGVLARVFPANVRYTGRSLAYQLSALVVGGGTPVLAQAILNSTGSI
VGVAIASGIYALVSLVCMIALLNRTGHRADALSSAERSDAAEWAAEPAGDAAAPRDDERRLKPAGETA

Sequences:

>Translated_468_residues
MSGPHTQPALPAKPGRAAFAAFVGTTIEWYDFYIYGTAAALVFGKVFFSRDMDAGVATLLAFVTFWAGFAARPLGGIVFG
HLGDRVGRKTALVITLVMMGLATTGIGLLPTYAQIGVWAPAGLVVLRVLQGIAVGGEWGGAVLIASENAPKHKSILYAAF
AQQGSPTGNLLATGAFFALSAMPTPDFLMWGWRIPFLLSAVLVIVGMVIRLKLEESADMQRVLARKRTVKLPLREVVRDH
WVVVLLAAGTLPVINVTYFRSTFALSWATKELGYAQGTFLGILSMSLVVQFLMQPVGAWFVSKVDMRRAMCWILIPEIVL
MPLMFHAVGTGSYWVAVAGMCISTIPSAMFYGAVGGVLARVFPANVRYTGRSLAYQLSALVVGGGTPVLAQAILNSTGSI
VGVAIASGIYALVSLVCMIALLNRTGHRADALSSAERSDAAEWAAEPAGDAAAPRDDERRLKPAGETA
>Mature_467_residues
SGPHTQPALPAKPGRAAFAAFVGTTIEWYDFYIYGTAAALVFGKVFFSRDMDAGVATLLAFVTFWAGFAARPLGGIVFGH
LGDRVGRKTALVITLVMMGLATTGIGLLPTYAQIGVWAPAGLVVLRVLQGIAVGGEWGGAVLIASENAPKHKSILYAAFA
QQGSPTGNLLATGAFFALSAMPTPDFLMWGWRIPFLLSAVLVIVGMVIRLKLEESADMQRVLARKRTVKLPLREVVRDHW
VVVLLAAGTLPVINVTYFRSTFALSWATKELGYAQGTFLGILSMSLVVQFLMQPVGAWFVSKVDMRRAMCWILIPEIVLM
PLMFHAVGTGSYWVAVAGMCISTIPSAMFYGAVGGVLARVFPANVRYTGRSLAYQLSALVVGGGTPVLAQAILNSTGSIV
GVAIASGIYALVSLVCMIALLNRTGHRADALSSAERSDAAEWAAEPAGDAAAPRDDERRLKPAGETA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=424, Percent_Identity=37.9716981132075, Blast_Score=250, Evalue=2e-67,
Organism=Escherichia coli, GI1789941, Length=437, Percent_Identity=38.2151029748284, Blast_Score=220, Evalue=2e-58,
Organism=Escherichia coli, GI1790550, Length=459, Percent_Identity=30.2832244008715, Blast_Score=191, Evalue=9e-50,
Organism=Escherichia coli, GI1788942, Length=405, Percent_Identity=31.1111111111111, Blast_Score=148, Evalue=8e-37,
Organism=Escherichia coli, GI87082231, Length=285, Percent_Identity=25.2631578947368, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87082404, Length=394, Percent_Identity=25.1269035532995, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 49684; Mature: 49553

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: PS00024 HEMOPEXIN ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGPHTQPALPAKPGRAAFAAFVGTTIEWYDFYIYGTAAALVFGKVFFSRDMDAGVATLL
CCCCCCCCCCCCCCCHHHHHHHHCCEEEHHEEEEHHHHHHHHHHHHHHHCCCCHHHHHHH
AFVTFWAGFAARPLGGIVFGHLGDRVGRKTALVITLVMMGLATTGIGLLPTYAQIGVWAP
HHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHCHHHHHCCCCH
AGLVVLRVLQGIAVGGEWGGAVLIASENAPKHKSILYAAFAQQGSPTGNLLATGAFFALS
HHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHC
AMPTPDFLMWGWRIPFLLSAVLVIVGMVIRLKLEESADMQRVLARKRTVKLPLREVVRDH
CCCCCCHHHHHCCHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHCCCCHHHHHHHH
WVVVLLAAGTLPVINVTYFRSTFALSWATKELGYAQGTFLGILSMSLVVQFLMQPVGAWF
HEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VSKVDMRRAMCWILIPEIVLMPLMFHAVGTGSYWVAVAGMCISTIPSAMFYGAVGGVLAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VFPANVRYTGRSLAYQLSALVVGGGTPVLAQAILNSTGSIVGVAIASGIYALVSLVCMIA
HHCCCCEECCHHHHHEEHEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
LLNRTGHRADALSSAERSDAAEWAAEPAGDAAAPRDDERRLKPAGETA
HHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCC
>Mature Secondary Structure 
SGPHTQPALPAKPGRAAFAAFVGTTIEWYDFYIYGTAAALVFGKVFFSRDMDAGVATLL
CCCCCCCCCCCCCCHHHHHHHHCCEEEHHEEEEHHHHHHHHHHHHHHHCCCCHHHHHHH
AFVTFWAGFAARPLGGIVFGHLGDRVGRKTALVITLVMMGLATTGIGLLPTYAQIGVWAP
HHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHCHHHHHCCCCH
AGLVVLRVLQGIAVGGEWGGAVLIASENAPKHKSILYAAFAQQGSPTGNLLATGAFFALS
HHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHC
AMPTPDFLMWGWRIPFLLSAVLVIVGMVIRLKLEESADMQRVLARKRTVKLPLREVVRDH
CCCCCCHHHHHCCHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHCCCCHHHHHHHH
WVVVLLAAGTLPVINVTYFRSTFALSWATKELGYAQGTFLGILSMSLVVQFLMQPVGAWF
HEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VSKVDMRRAMCWILIPEIVLMPLMFHAVGTGSYWVAVAGMCISTIPSAMFYGAVGGVLAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VFPANVRYTGRSLAYQLSALVVGGGTPVLAQAILNSTGSIVGVAIASGIYALVSLVCMIA
HHCCCCEECCHHHHHEEHEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
LLNRTGHRADALSSAERSDAAEWAAEPAGDAAAPRDDERRLKPAGETA
HHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]