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The map label for this gene is gcvA [H]

Identifier: 161524270

GI number: 161524270

Start: 1196879

End: 1197820

Strand: Reverse

Name: gcvA [H]

Synonym: Bmul_1094

Alternate gene names: 161524270

Gene position: 1197820-1196879 (Counterclockwise)

Preceding gene: 161524284

Following gene: 161524269

Centisome position: 34.73

GC content: 72.4

Gene sequence:

>942_bases
ATGTCAACGCCGCTCGTTCGCCTTCCGTCGCTCGATCTGGTGCGCGGCTTCGTCGCCGTCGGCCGCCGGATGAGCATTAC
GCTCGCCGCCCAGGATCTGTGCGTCACGCAGTCGGCCGTGAGCCGGCAGATTCATGCGCTCGAAGCGCATCTCGGCGTCG
CGCTGCTGCGGCGCGGCTACCGGACGATCGCCTTCACGCCCGAAGGCGAGCGGCTGTTCCGCGCGGCCGATGCGGCGCTG
CACAGCCTGCAGGACACCGTCGCGTCGCTCGCCGTCGCGCGCGAGCGTCAGCCGGTCACGATCACCGCGAGCATCGGCGT
CACGGCACTGTGGCTGCTGCCGCGGCTCGGCGGCCTGCAAGCGCGGTTGCCGGACGTCGATCTGCGCGTCGCCGCGAACG
ACAAGGTACTCGATTTGCGCGCCGAAGGCATCGATCTCGCGATCCGCTACTGCCCGCGCGATCGCGCGCCGGCCGGCGCG
CTGCGGCTGTTCGACGAGATCGTGCTGCCGGTCGCGCACCCGGCGCTCGCCGCGCGTCCGCTCGTCGATGCCGCCGCGCT
CGCCGAGCACGTGCTGCTCGAATTCGACGGGCCGCCGCAAGCGCAGCTGCAATGGCAAGCGCACCTACGCGCGGCCGGGC
TTGGCGATGCGCGGCCGAAGGGCGTGCTGCGCTTCAACCAGTACGATCAGGTGATTCAGGCCGCGATCGCCGGCCAGGGC
GTCGCGCTCGGGCGCCTCGCGCTGGTCGCGCCGATGCTGTCGGACGGCCGGCTCGCGGTGCTCGGCCCGCGCATCCGCGC
GCGGCCCGATACATACGGCTACTGGCTCCACCAGCACGATCCGGCGCCGCGCCGCGAAGTTGCCGACGTGCGCGACTGGA
TGCTCGCCGAAGCGGCCGAGTGCGACGTCGCGATACGCGCGCACGATGCGGCGCGCGACTGA

Upstream 100 bases:

>100_bases
CCGCGCGGTCGCCGATGCGCGCCGCGTCGCCCGTCCATTGAACTTGACGGGCGCCGCGTTCTCAACCAATCTTGCTGCTC
CGTTCGCATGAGCCCCACGC

Downstream 100 bases:

>100_bases
GGCGGCCGGGGCGGCGCACGAGACTTCCTCTATACGGACTGCACCGCACGACACCCACCGCGACGCCGGCACACCGCGGC
TCGCATTCCCGCCACGCATG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MSTPLVRLPSLDLVRGFVAVGRRMSITLAAQDLCVTQSAVSRQIHALEAHLGVALLRRGYRTIAFTPEGERLFRAADAAL
HSLQDTVASLAVARERQPVTITASIGVTALWLLPRLGGLQARLPDVDLRVAANDKVLDLRAEGIDLAIRYCPRDRAPAGA
LRLFDEIVLPVAHPALAARPLVDAAALAEHVLLEFDGPPQAQLQWQAHLRAAGLGDARPKGVLRFNQYDQVIQAAIAGQG
VALGRLALVAPMLSDGRLAVLGPRIRARPDTYGYWLHQHDPAPRREVADVRDWMLAEAAECDVAIRAHDAARD

Sequences:

>Translated_313_residues
MSTPLVRLPSLDLVRGFVAVGRRMSITLAAQDLCVTQSAVSRQIHALEAHLGVALLRRGYRTIAFTPEGERLFRAADAAL
HSLQDTVASLAVARERQPVTITASIGVTALWLLPRLGGLQARLPDVDLRVAANDKVLDLRAEGIDLAIRYCPRDRAPAGA
LRLFDEIVLPVAHPALAARPLVDAAALAEHVLLEFDGPPQAQLQWQAHLRAAGLGDARPKGVLRFNQYDQVIQAAIAGQG
VALGRLALVAPMLSDGRLAVLGPRIRARPDTYGYWLHQHDPAPRREVADVRDWMLAEAAECDVAIRAHDAARD
>Mature_312_residues
STPLVRLPSLDLVRGFVAVGRRMSITLAAQDLCVTQSAVSRQIHALEAHLGVALLRRGYRTIAFTPEGERLFRAADAALH
SLQDTVASLAVARERQPVTITASIGVTALWLLPRLGGLQARLPDVDLRVAANDKVLDLRAEGIDLAIRYCPRDRAPAGAL
RLFDEIVLPVAHPALAARPLVDAAALAEHVLLEFDGPPQAQLQWQAHLRAAGLGDARPKGVLRFNQYDQVIQAAIAGQGV
ALGRLALVAPMLSDGRLAVLGPRIRARPDTYGYWLHQHDPAPRREVADVRDWMLAEAAECDVAIRAHDAARD

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=296, Percent_Identity=33.1081081081081, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1788706, Length=256, Percent_Identity=30.078125, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1786448, Length=295, Percent_Identity=31.5254237288136, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786401, Length=257, Percent_Identity=27.6264591439689, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87081978, Length=265, Percent_Identity=30.9433962264151, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI145693193, Length=277, Percent_Identity=27.7978339350181, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1787589, Length=266, Percent_Identity=26.6917293233083, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI157672245, Length=272, Percent_Identity=29.7794117647059, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1789639, Length=268, Percent_Identity=27.2388059701493, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33920; Mature: 33788

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPLVRLPSLDLVRGFVAVGRRMSITLAAQDLCVTQSAVSRQIHALEAHLGVALLRRGY
CCCCCEECCCHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RTIAFTPEGERLFRAADAALHSLQDTVASLAVARERQPVTITASIGVTALWLLPRLGGLQ
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHCCCCCC
ARLPDVDLRVAANDKVLDLRAEGIDLAIRYCPRDRAPAGALRLFDEIVLPVAHPALAARP
CCCCCCEEEEECCCCEEEEEECCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHH
LVDAAALAEHVLLEFDGPPQAQLQWQAHLRAAGLGDARPKGVLRFNQYDQVIQAAIAGQG
HHHHHHHHHHHEEECCCCCCCCEEHEEHHHHCCCCCCCCCCEEEECHHHHHHHHHHCCCC
VALGRLALVAPMLSDGRLAVLGPRIRARPDTYGYWLHQHDPAPRREVADVRDWMLAEAAE
HHHHHHHHHHHHCCCCCEEEECCCEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHC
CDVAIRAHDAARD
CCEEEEECCCCCC
>Mature Secondary Structure 
STPLVRLPSLDLVRGFVAVGRRMSITLAAQDLCVTQSAVSRQIHALEAHLGVALLRRGY
CCCCEECCCHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RTIAFTPEGERLFRAADAALHSLQDTVASLAVARERQPVTITASIGVTALWLLPRLGGLQ
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHCCCCCC
ARLPDVDLRVAANDKVLDLRAEGIDLAIRYCPRDRAPAGALRLFDEIVLPVAHPALAARP
CCCCCCEEEEECCCCEEEEEECCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHH
LVDAAALAEHVLLEFDGPPQAQLQWQAHLRAAGLGDARPKGVLRFNQYDQVIQAAIAGQG
HHHHHHHHHHHEEECCCCCCCCEEHEEHHHHCCCCCCCCCCEEEECHHHHHHHHHHCCCC
VALGRLALVAPMLSDGRLAVLGPRIRARPDTYGYWLHQHDPAPRREVADVRDWMLAEAAE
HHHHHHHHHHHHCCCCCEEEECCCEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHC
CDVAIRAHDAARD
CCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]