Click here to switch to the map view.

The map label for this gene is fumB [H]

Identifier: 161524258

GI number: 161524258

Start: 1179894

End: 1181417

Strand: Reverse

Name: fumB [H]

Synonym: Bmul_1082

Alternate gene names: 161524258

Gene position: 1181417-1179894 (Counterclockwise)

Preceding gene: 161524259

Following gene: 161524257

Centisome position: 34.26

GC content: 65.09

Gene sequence:

>1524_bases
ATGACCGTCATCAAACAGGAAGACCTGATCCAGAGCATCGCGGATTCGCTGCAATACATCAGCTACTACCATCCGCTCGA
CTACATCCAGGCGCTCGGCCGCGCATACGAGCTCGAAGAGAGCCCGGCCGCGAAGGACGCGATCGCGCAGATCCTCACGA
ACAGCCGCATGTGTGCGGAAGGCCGCCGCCCGATCTGCCAGGACACCGGGATCGTGACGATCTTCGTGAAGGTCGGCATG
GACGTGCGCTGGGACGGCGCGACGATGAGCGTGACCGACATGATCAACGAAGGCGTGCGCCGCGGCTACACGCATCCGGA
CAACGTGCTGCGCGCATCGATCGTGAATCCGCCGGAAGGCGCGCGCAAGAACACGAAGGACAATACGCCGGCGGTGATCC
ACTACGAGATCGTGCCGGGCGACAAGGTCGACGTGCAGGTCGCGGCCAAGGGCGGCGGCTCCGAGAACAAGTCGAAGTTC
GTGATGCTCAATCCGTCCGACTCGATCGTCGACTGGGTGCTGAAGACCGTGCCGACGATGGGCGCGGGCTGGTGCCCGCC
CGGCATGCTCGGGATCGGCATCGGCGGCACCGCCGAGAAGGCGATGCTGATGGCGAAGGAATCGCTGATGGATCCGATCG
ACATCCAGGAAATCATCGCGCGCGGCCCGCAGGACTGGATCGAGGAACTGCGCGTCGAACTGCACGAGAAGGTGAACGCG
CTCGGCATCGGCGCGCAAGGTCTCGGCGGCCTGTCGACCGTGCTCGACGTGAAGATCATGGCGGCGCCGACGCATGCGGC
ATCGAAGCCGGTCGCGCTGATCCCGAACTGCGCGGCCACGCGCCACGCGCACTTCGTGCTCGACGGCTCGGGCCCCGCGA
AGCTCGAGGCGCCGTCGCTCGACGCATGGCCGAAGGTCAACTGGGAGCCGAACACCGAAACGAGCAAGCGCGTCGACCTG
AACACGCTGACGCCGGAAGAAGTGGCGAGCTGGAAGCCGGGCCAGACGCTGCTGCTGTCGGGCAAGATGCTGACGGGCCG
CGACGCTGCGCACAAGCGCATCGCCGACATGCTCGCGAAGGGCGAGAAGCTGCCGGTCGACTTCACGAACCGCGTGATCT
ACTACGTCGGCCCGGTCGATCCGGTGCGCGACGAAGTGGTCGGCCCGGCGGGCCCGACGACCGCCACGCGGATGGACAAG
TTCACCGAGACGATGCTCGCGCAGACGGGCCTGATCTCGATGATCGGCAAGGCCGAGCGCGGGCCGGTCGCGATCGAGGC
GATCAAGAAGCACAAGGCGGCCTATCTGATGGCGGTCGGCGGCGCCGCGTACCTCGTGTCGAAGGCGATCCGCGACGCGA
AGGTGATCGCATTCGAAGATCTCGGGATGGAAGCGATCTACGAATTCGACGTGAAGGACATGCCTGTAACAGTTGCTGTC
GATTCTTCCGGCACGTCGGTGCATCAGACCGGTCCGAAGGAATGGCAGGCGAAGATCGGCAAGATCCCGGTCGCGACTGC
TTAA

Upstream 100 bases:

>100_bases
CACCCGGCCCATTTCCACGAGACGCAGACAGCCGCTGCGTCGCGCACGCCGGCCTGAACGGCCGGCCCAGCATTCCTACT
GTCCCCACAGAGCCTCAGCC

Downstream 100 bases:

>100_bases
GCGCCACGACGCGAAAGGCCGGACAGACGCTGTCCGGCCTTTTTCATTCCGGTTTGCATTCACGCGTATCGCGAATCGTT
TCTTGCGGACCGTGCGCGGT

Product: tartrate/fumarate subfamily Fe-S type hydro-lyase subunit alpha

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 507; Mature: 506

Protein sequence:

>507_residues
MTVIKQEDLIQSIADSLQYISYYHPLDYIQALGRAYELEESPAAKDAIAQILTNSRMCAEGRRPICQDTGIVTIFVKVGM
DVRWDGATMSVTDMINEGVRRGYTHPDNVLRASIVNPPEGARKNTKDNTPAVIHYEIVPGDKVDVQVAAKGGGSENKSKF
VMLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAMLMAKESLMDPIDIQEIIARGPQDWIEELRVELHEKVNA
LGIGAQGLGGLSTVLDVKIMAAPTHAASKPVALIPNCAATRHAHFVLDGSGPAKLEAPSLDAWPKVNWEPNTETSKRVDL
NTLTPEEVASWKPGQTLLLSGKMLTGRDAAHKRIADMLAKGEKLPVDFTNRVIYYVGPVDPVRDEVVGPAGPTTATRMDK
FTETMLAQTGLISMIGKAERGPVAIEAIKKHKAAYLMAVGGAAYLVSKAIRDAKVIAFEDLGMEAIYEFDVKDMPVTVAV
DSSGTSVHQTGPKEWQAKIGKIPVATA

Sequences:

>Translated_507_residues
MTVIKQEDLIQSIADSLQYISYYHPLDYIQALGRAYELEESPAAKDAIAQILTNSRMCAEGRRPICQDTGIVTIFVKVGM
DVRWDGATMSVTDMINEGVRRGYTHPDNVLRASIVNPPEGARKNTKDNTPAVIHYEIVPGDKVDVQVAAKGGGSENKSKF
VMLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAMLMAKESLMDPIDIQEIIARGPQDWIEELRVELHEKVNA
LGIGAQGLGGLSTVLDVKIMAAPTHAASKPVALIPNCAATRHAHFVLDGSGPAKLEAPSLDAWPKVNWEPNTETSKRVDL
NTLTPEEVASWKPGQTLLLSGKMLTGRDAAHKRIADMLAKGEKLPVDFTNRVIYYVGPVDPVRDEVVGPAGPTTATRMDK
FTETMLAQTGLISMIGKAERGPVAIEAIKKHKAAYLMAVGGAAYLVSKAIRDAKVIAFEDLGMEAIYEFDVKDMPVTVAV
DSSGTSVHQTGPKEWQAKIGKIPVATA
>Mature_506_residues
TVIKQEDLIQSIADSLQYISYYHPLDYIQALGRAYELEESPAAKDAIAQILTNSRMCAEGRRPICQDTGIVTIFVKVGMD
VRWDGATMSVTDMINEGVRRGYTHPDNVLRASIVNPPEGARKNTKDNTPAVIHYEIVPGDKVDVQVAAKGGGSENKSKFV
MLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAMLMAKESLMDPIDIQEIIARGPQDWIEELRVELHEKVNAL
GIGAQGLGGLSTVLDVKIMAAPTHAASKPVALIPNCAATRHAHFVLDGSGPAKLEAPSLDAWPKVNWEPNTETSKRVDLN
TLTPEEVASWKPGQTLLLSGKMLTGRDAAHKRIADMLAKGEKLPVDFTNRVIYYVGPVDPVRDEVVGPAGPTTATRMDKF
TETMLAQTGLISMIGKAERGPVAIEAIKKHKAAYLMAVGGAAYLVSKAIRDAKVIAFEDLGMEAIYEFDVKDMPVTVAVD
SSGTSVHQTGPKEWQAKIGKIPVATA

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1787897, Length=467, Percent_Identity=32.5481798715203, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI1790564, Length=466, Percent_Identity=31.3304721030043, Blast_Score=195, Evalue=6e-51,
Organism=Escherichia coli, GI1789442, Length=284, Percent_Identity=29.2253521126761, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1789443, Length=179, Percent_Identity=27.9329608938547, Blast_Score=86, Evalue=6e-18,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 54597; Mature: 54465

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVIKQEDLIQSIADSLQYISYYHPLDYIQALGRAYELEESPAAKDAIAQILTNSRMCAE
CCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHEECCCCCCHHHHHHHHHHCCCHHHC
GRRPICQDTGIVTIFVKVGMDVRWDGATMSVTDMINEGVRRGYTHPDNVLRASIVNPPEG
CCCCCCCCCCEEEEEEEECCEEEECCCCCHHHHHHHHHHHCCCCCCCHHHEEECCCCCCC
ARKNTKDNTPAVIHYEIVPGDKVDVQVAAKGGGSENKSKFVMLNPSDSIVDWVLKTVPTM
CCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHCCC
GAGWCPPGMLGIGIGGTAEKAMLMAKESLMDPIDIQEIIARGPQDWIEELRVELHEKVNA
CCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHH
LGIGAQGLGGLSTVLDVKIMAAPTHAASKPVALIPNCAATRHAHFVLDGSGPAKLEAPSL
EECCCCCCCCHHHHHEEEEEECCCCCCCCCEEECCCCCCCCCEEEEEECCCCCEECCCCC
DAWPKVNWEPNTETSKRVDLNTLTPEEVASWKPGQTLLLSGKMLTGRDAAHKRIADMLAK
CCCCCCCCCCCCCCCCEEEECCCCHHHHHCCCCCCEEEEECCEECCCHHHHHHHHHHHHC
GEKLPVDFTNRVIYYVGPVDPVRDEVVGPAGPTTATRMDKFTETMLAQTGLISMIGKAER
CCCCCEEECCCEEEEECCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
GPVAIEAIKKHKAAYLMAVGGAAYLVSKAIRDAKVIAFEDLGMEAIYEFDVKDMPVTVAV
CCCHHHHHHHCCCEEEEEECHHHHHHHHHHCCCEEEEEECCCCCEEEEECCCCCCEEEEE
DSSGTSVHQTGPKEWQAKIGKIPVATA
CCCCCCCCCCCCHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TVIKQEDLIQSIADSLQYISYYHPLDYIQALGRAYELEESPAAKDAIAQILTNSRMCAE
CCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHEECCCCCCHHHHHHHHHHCCCHHHC
GRRPICQDTGIVTIFVKVGMDVRWDGATMSVTDMINEGVRRGYTHPDNVLRASIVNPPEG
CCCCCCCCCCEEEEEEEECCEEEECCCCCHHHHHHHHHHHCCCCCCCHHHEEECCCCCCC
ARKNTKDNTPAVIHYEIVPGDKVDVQVAAKGGGSENKSKFVMLNPSDSIVDWVLKTVPTM
CCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHCCC
GAGWCPPGMLGIGIGGTAEKAMLMAKESLMDPIDIQEIIARGPQDWIEELRVELHEKVNA
CCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHH
LGIGAQGLGGLSTVLDVKIMAAPTHAASKPVALIPNCAATRHAHFVLDGSGPAKLEAPSL
EECCCCCCCCHHHHHEEEEEECCCCCCCCCEEECCCCCCCCCEEEEEECCCCCEECCCCC
DAWPKVNWEPNTETSKRVDLNTLTPEEVASWKPGQTLLLSGKMLTGRDAAHKRIADMLAK
CCCCCCCCCCCCCCCCEEEECCCCHHHHHCCCCCCEEEEECCEECCCHHHHHHHHHHHHC
GEKLPVDFTNRVIYYVGPVDPVRDEVVGPAGPTTATRMDKFTETMLAQTGLISMIGKAER
CCCCCEEECCCEEEEECCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
GPVAIEAIKKHKAAYLMAVGGAAYLVSKAIRDAKVIAFEDLGMEAIYEFDVKDMPVTVAV
CCCHHHHHHHCCCEEEEEECHHHHHHHHHHCCCEEEEEECCCCCEEEEECCCCCCEEEEE
DSSGTSVHQTGPKEWQAKIGKIPVATA
CCCCCCCCCCCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]