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The map label for this gene is purD [H]

Identifier: 161524159

GI number: 161524159

Start: 1076900

End: 1078177

Strand: Reverse

Name: purD [H]

Synonym: Bmul_0983

Alternate gene names: 161524159

Gene position: 1078177-1076900 (Counterclockwise)

Preceding gene: 161524160

Following gene: 161524158

Centisome position: 31.27

GC content: 66.9

Gene sequence:

>1278_bases
ATGAAACTACTCGTCGTCGGTTCCGGTGGCCGCGAACATGCGCTCGCGTGGAAGCTCGCGCAATCGCCGCGCGTCCAGAT
GGTCTATGTCGCGCCCGGCAATGGCGGCACGGCGCAGGACGAGCGTCTGAAGAACGTCGACATCACGTCGCTCGACGCGC
TCGCCGACTTCGCGGAAAGCGAAGGCGTCGCATTCACGCTCGTCGGGCCCGAAGCGCCGCTCGCGGCCGGCATCGTCAAC
CTGTTCCGCGCGCGCGGCCTGAAGATCTTCGGGCCGACGCGCGAAGCCGCGCAGCTCGAGAGCTCGAAGGATTTCGCGAA
GGCATTCATGAAGCGGCACGGCATTCCGACCGCCGACTACGAGACCTTCTCGGACGCGGCCGCCGCGCACGCCTACATCG
ACGCGAAAGGCGCGCCGATCGTCGTGAAGGCCGACGGCCTCGCGGCCGGCAAGGGCGTGGTCGTCGCGATGACGCTCGAG
GAAGCGCACGAAGCGGTCGACATGATGCTGTCGGGCAACAAGCTCGGCGACGCCGGCGCACGCGTCGTGATCGAGGAATT
CCTCGACGGCGAGGAAGCGAGCTTCATCGTGATGGTCGACGGCAAGCACGCGCTCGCACTCGCCTCGAGCCAGGACCACA
AGCGTCTGCTCGACGAGGATCGCGGCCCGAACACGGGCGGCATGGGCGCGTATTCGCCGACGCCGATCGTCACGCCGCAG
ATGCATGCGCGCGTGATGCGCGAGATCATCATGCCGACCGTGCGCGGGATGGAGAAGGACGGCATCCGCTTCACGGGCTT
CCTGTATGCCGGCCTGATGATCGACAAGGAAGGCAATCCGCGCACGCTCGAATTCAACTGCCGGATGGGCGACCCGGAAA
CGCAGCCGATCATGGCGCGCCTGAAGAGCGATTTCTCGAAGGTCGTCGAGCAGGCGATCGCGGGCACGCTCGATACGGTC
GAGCTCGACTGGGATCGCCGCACCGCGCTCGGCGTCGTGCTCGCCGCGCACGGCTACCCCGATGCGCCGCGCAAGGGCGA
CCGCATCAACGGCATCCCGGCGGAAACCGAACAGGCCGTGACGTTCCATGCGGGCACGACGCTCGACGGCGACAAGCTGG
TCACGTCGGGCGGCCGCGTGCTGTGCGTGGTCGGCCTCGCCGATTCGGTGCGCGAAGCGCAGCAGCATGCGTACGACACG
ATCAACCAGATCAATTTCGAAGGCATGCAGTATCGCCGCGACATCGGCTACCGCGCACTCAGCCGCAAGAGCGCATGA

Upstream 100 bases:

>100_bases
CCGGCCGCCGCGCGCACGTCGCAGCGGCGGCCCGCCCGGTTTCGCGGCCGGCGCGCCTGAGCGTGCCGGCCGCCCTGTTT
TCTAGTCTGCGGGGAATCCC

Downstream 100 bases:

>100_bases
CGCCGGCGGCCGGCGCACCGCCGCGCGGTGCGTCGTGCCGGCTGCCGCGGTTCGCTAGAATGCGCGGCAGAAGCCTTTTT
TACGGCCCCCGCCGCGCGCG

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MKLLVVGSGGREHALAWKLAQSPRVQMVYVAPGNGGTAQDERLKNVDITSLDALADFAESEGVAFTLVGPEAPLAAGIVN
LFRARGLKIFGPTREAAQLESSKDFAKAFMKRHGIPTADYETFSDAAAAHAYIDAKGAPIVVKADGLAAGKGVVVAMTLE
EAHEAVDMMLSGNKLGDAGARVVIEEFLDGEEASFIVMVDGKHALALASSQDHKRLLDEDRGPNTGGMGAYSPTPIVTPQ
MHARVMREIIMPTVRGMEKDGIRFTGFLYAGLMIDKEGNPRTLEFNCRMGDPETQPIMARLKSDFSKVVEQAIAGTLDTV
ELDWDRRTALGVVLAAHGYPDAPRKGDRINGIPAETEQAVTFHAGTTLDGDKLVTSGGRVLCVVGLADSVREAQQHAYDT
INQINFEGMQYRRDIGYRALSRKSA

Sequences:

>Translated_425_residues
MKLLVVGSGGREHALAWKLAQSPRVQMVYVAPGNGGTAQDERLKNVDITSLDALADFAESEGVAFTLVGPEAPLAAGIVN
LFRARGLKIFGPTREAAQLESSKDFAKAFMKRHGIPTADYETFSDAAAAHAYIDAKGAPIVVKADGLAAGKGVVVAMTLE
EAHEAVDMMLSGNKLGDAGARVVIEEFLDGEEASFIVMVDGKHALALASSQDHKRLLDEDRGPNTGGMGAYSPTPIVTPQ
MHARVMREIIMPTVRGMEKDGIRFTGFLYAGLMIDKEGNPRTLEFNCRMGDPETQPIMARLKSDFSKVVEQAIAGTLDTV
ELDWDRRTALGVVLAAHGYPDAPRKGDRINGIPAETEQAVTFHAGTTLDGDKLVTSGGRVLCVVGLADSVREAQQHAYDT
INQINFEGMQYRRDIGYRALSRKSA
>Mature_425_residues
MKLLVVGSGGREHALAWKLAQSPRVQMVYVAPGNGGTAQDERLKNVDITSLDALADFAESEGVAFTLVGPEAPLAAGIVN
LFRARGLKIFGPTREAAQLESSKDFAKAFMKRHGIPTADYETFSDAAAAHAYIDAKGAPIVVKADGLAAGKGVVVAMTLE
EAHEAVDMMLSGNKLGDAGARVVIEEFLDGEEASFIVMVDGKHALALASSQDHKRLLDEDRGPNTGGMGAYSPTPIVTPQ
MHARVMREIIMPTVRGMEKDGIRFTGFLYAGLMIDKEGNPRTLEFNCRMGDPETQPIMARLKSDFSKVVEQAIAGTLDTV
ELDWDRRTALGVVLAAHGYPDAPRKGDRINGIPAETEQAVTFHAGTTLDGDKLVTSGGRVLCVVGLADSVREAQQHAYDT
INQINFEGMQYRRDIGYRALSRKSA

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=425, Percent_Identity=49.8823529411765, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI4503915, Length=425, Percent_Identity=49.8823529411765, Blast_Score=401, Evalue=1e-112,
Organism=Homo sapiens, GI209869995, Length=425, Percent_Identity=49.8823529411765, Blast_Score=401, Evalue=1e-112,
Organism=Homo sapiens, GI209869993, Length=425, Percent_Identity=49.8823529411765, Blast_Score=401, Evalue=1e-112,
Organism=Escherichia coli, GI1790438, Length=428, Percent_Identity=60.7476635514019, Blast_Score=522, Evalue=1e-149,
Organism=Caenorhabditis elegans, GI17567511, Length=421, Percent_Identity=45.1306413301663, Blast_Score=353, Evalue=9e-98,
Organism=Saccharomyces cerevisiae, GI6321203, Length=449, Percent_Identity=41.8708240534521, Blast_Score=344, Evalue=1e-95,
Organism=Drosophila melanogaster, GI62471619, Length=430, Percent_Identity=46.046511627907, Blast_Score=357, Evalue=9e-99,
Organism=Drosophila melanogaster, GI24582400, Length=435, Percent_Identity=45.9770114942529, Blast_Score=357, Evalue=1e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45656; Mature: 45656

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLVVGSGGREHALAWKLAQSPRVQMVYVAPGNGGTAQDERLKNVDITSLDALADFAES
CEEEEEECCCCCCEEEEEECCCCCEEEEEEECCCCCCCCHHHHCCCCCCHHHHHHHHHCC
EGVAFTLVGPEAPLAAGIVNLFRARGLKIFGPTREAAQLESSKDFAKAFMKRHGIPTADY
CCEEEEEECCCCCHHHHHHHHHHHCCEEEECCCHHHHHHHCCHHHHHHHHHHCCCCCCCC
ETFSDAAAAHAYIDAKGAPIVVKADGLAAGKGVVVAMTLEEAHEAVDMMLSGNKLGDAGA
CHHHHHHHHEEEEECCCCEEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCCHH
RVVIEEFLDGEEASFIVMVDGKHALALASSQDHKRLLDEDRGPNTGGMGAYSPTPIVTPQ
HHHHHHHCCCCCCEEEEEECCCEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCHH
MHARVMREIIMPTVRGMEKDGIRFTGFLYAGLMIDKEGNPRTLEFNCRMGDPETQPIMAR
HHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEECCCCCCEEEEEEECCCCCCHHHHHH
LKSDFSKVVEQAIAGTLDTVELDWDRRTALGVVLAAHGYPDAPRKGDRINGIPAETEQAV
HHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEE
TFHAGTTLDGDKLVTSGGRVLCVVGLADSVREAQQHAYDTINQINFEGMQYRRDIGYRAL
EEECCCCCCCCEEEECCCCEEEEEECHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
SRKSA
HCCCC
>Mature Secondary Structure
MKLLVVGSGGREHALAWKLAQSPRVQMVYVAPGNGGTAQDERLKNVDITSLDALADFAES
CEEEEEECCCCCCEEEEEECCCCCEEEEEEECCCCCCCCHHHHCCCCCCHHHHHHHHHCC
EGVAFTLVGPEAPLAAGIVNLFRARGLKIFGPTREAAQLESSKDFAKAFMKRHGIPTADY
CCEEEEEECCCCCHHHHHHHHHHHCCEEEECCCHHHHHHHCCHHHHHHHHHHCCCCCCCC
ETFSDAAAAHAYIDAKGAPIVVKADGLAAGKGVVVAMTLEEAHEAVDMMLSGNKLGDAGA
CHHHHHHHHEEEEECCCCEEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCCHH
RVVIEEFLDGEEASFIVMVDGKHALALASSQDHKRLLDEDRGPNTGGMGAYSPTPIVTPQ
HHHHHHHCCCCCCEEEEEECCCEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCHH
MHARVMREIIMPTVRGMEKDGIRFTGFLYAGLMIDKEGNPRTLEFNCRMGDPETQPIMAR
HHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEECCCCCCEEEEEEECCCCCCHHHHHH
LKSDFSKVVEQAIAGTLDTVELDWDRRTALGVVLAAHGYPDAPRKGDRINGIPAETEQAV
HHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEE
TFHAGTTLDGDKLVTSGGRVLCVVGLADSVREAQQHAYDTINQINFEGMQYRRDIGYRAL
EEECCCCCCCCEEEECCCCEEEEEECHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
SRKSA
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]