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The map label for this gene is purT [H]

Identifier: 161524134

GI number: 161524134

Start: 1050166

End: 1051380

Strand: Direct

Name: purT [H]

Synonym: Bmul_0958

Alternate gene names: 161524134

Gene position: 1050166-1051380 (Clockwise)

Preceding gene: 161524131

Following gene: 161524135

Centisome position: 30.45

GC content: 69.88

Gene sequence:

>1215_bases
ATGCAGATCGGTCAGCGGCTCGGTACGCCGCTTTCGCCGTCGGCCACGCGCGTCATGCTGCTCGGCGCCGGCGAGCTCGG
CAAGGAAGTCGTCATCGCGTTGCAGCGACTCGGCGTCGAAGTCATCGCGGTCGACCGTTACCCGGATGCGCCGGGCCATC
AGGTCGCGCACCGCGCCCACGTGATCGACATGACGGACGGCGCCGCGCTGCGCGCGCTCGTCGAGGCCGAGCGTCCGCAC
CTGATCGTGCCGGAGATCGAGGCGATCGCGACCGACGCGCTCGCGCAGATCGAGGCGGCCGGGCTTGCCGAAGTGATCCC
GACCGCGCGCGCGACGCAATTGACGATGAACCGCGAAGGCATCCGCCGGCTCGCGGCCGAAACGCTGGGGCTGCCGACGT
CGCCGTATGCGTTCGCCGAGTCGTTCGACGAGTTCGCGGCCGCGGTCGCGCAGATCGGCATGCCGTGCGTCGTGAAGCCC
GTGATGTCGTCGTCGGGCAAGGGGCAGTCGGTCGTGCGGACCGAAGCCGACGTGAAGCCCGCGTGGGACTACGCGATGGC
GGGCGGCCGCGTGAATCACGGTCGCGTGATCGTTGAGGGCTTCATCGATTTCGAATACGAGATCACGCAGCTGACCGTGC
GCGCGATCGATCCCGCGACGCTCGACACGCGTACGTGGTTCTGCGAGCCGATCGGCCATGTGCAGGTCGCGGGCGATTAC
GTCGAATCGTGGCAGCCGCAGCCGATGCGCGCGGCGGCGCTCGAGAAATCGCGCGAGATTGCGCACAAGGTCACCGAGGC
GCTCGGCGGGCGCGGCCTGTTCGGCGTCGAGCTGTTCGTGCGCGGCGACGAAGTCTGGTTCTCGGAAGTAAGCCCGCGGC
CGCACGATACGGGGCTCGTCACGCTCGCATCGCAGCGCCAGTCGGAGTTCGAATTGCACGCGCGCGCGATTCTCGGGCTG
CCGGTCGACCCGACGCTCGGTTCGCCGGCCGCATCGGCCGTGATCTATGGCGGGCTCGACGCGCGCGGCATCGCCTTCGA
AGGCGTGCGCGACGCGCTCGCGGTGCCGGGCGCCGATCTGCGACTGTTCGGCAAGCCGGAAAGCTTCGCGAAACGGCGCA
TGGGTGTCGCGCTTGCAACGGGCGCGAACGTCGACGAAGCGCGCGAGCGCGCCAAACGTGCGGCCGCGGCCGTCCGGCCC
GTAGCGGGCAACTGA

Upstream 100 bases:

>100_bases
GCCGCGCGCCGCATCCGACGGCGGCGAAGCGCGCGAAACGGCCCGGCCCGCGTGTTAACATCGGAGCCGTTTTCGTCCTC
CACCAGAAGCGGGAAATCAC

Downstream 100 bases:

>100_bases
TCAACCGGGAGTGCATCATGGCGCCGATCTGGATGCGTATCGCAAAACTGGCCGCCGCGGCGGCGCTTGCGGCGGGCCTT
GCCGGCTGCGGGCTGGCCGC

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2 [H]

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MQIGQRLGTPLSPSATRVMLLGAGELGKEVVIALQRLGVEVIAVDRYPDAPGHQVAHRAHVIDMTDGAALRALVEAERPH
LIVPEIEAIATDALAQIEAAGLAEVIPTARATQLTMNREGIRRLAAETLGLPTSPYAFAESFDEFAAAVAQIGMPCVVKP
VMSSSGKGQSVVRTEADVKPAWDYAMAGGRVNHGRVIVEGFIDFEYEITQLTVRAIDPATLDTRTWFCEPIGHVQVAGDY
VESWQPQPMRAAALEKSREIAHKVTEALGGRGLFGVELFVRGDEVWFSEVSPRPHDTGLVTLASQRQSEFELHARAILGL
PVDPTLGSPAASAVIYGGLDARGIAFEGVRDALAVPGADLRLFGKPESFAKRRMGVALATGANVDEARERAKRAAAAVRP
VAGN

Sequences:

>Translated_404_residues
MQIGQRLGTPLSPSATRVMLLGAGELGKEVVIALQRLGVEVIAVDRYPDAPGHQVAHRAHVIDMTDGAALRALVEAERPH
LIVPEIEAIATDALAQIEAAGLAEVIPTARATQLTMNREGIRRLAAETLGLPTSPYAFAESFDEFAAAVAQIGMPCVVKP
VMSSSGKGQSVVRTEADVKPAWDYAMAGGRVNHGRVIVEGFIDFEYEITQLTVRAIDPATLDTRTWFCEPIGHVQVAGDY
VESWQPQPMRAAALEKSREIAHKVTEALGGRGLFGVELFVRGDEVWFSEVSPRPHDTGLVTLASQRQSEFELHARAILGL
PVDPTLGSPAASAVIYGGLDARGIAFEGVRDALAVPGADLRLFGKPESFAKRRMGVALATGANVDEARERAKRAAAAVRP
VAGN
>Mature_404_residues
MQIGQRLGTPLSPSATRVMLLGAGELGKEVVIALQRLGVEVIAVDRYPDAPGHQVAHRAHVIDMTDGAALRALVEAERPH
LIVPEIEAIATDALAQIEAAGLAEVIPTARATQLTMNREGIRRLAAETLGLPTSPYAFAESFDEFAAAVAQIGMPCVVKP
VMSSSGKGQSVVRTEADVKPAWDYAMAGGRVNHGRVIVEGFIDFEYEITQLTVRAIDPATLDTRTWFCEPIGHVQVAGDY
VESWQPQPMRAAALEKSREIAHKVTEALGGRGLFGVELFVRGDEVWFSEVSPRPHDTGLVTLASQRQSEFELHARAILGL
PVDPTLGSPAASAVIYGGLDARGIAFEGVRDALAVPGADLRLFGKPESFAKRRMGVALATGANVDEARERAKRAAAAVRP
VAGN

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP [H]

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1788155, Length=396, Percent_Identity=63.8888888888889, Blast_Score=464, Evalue=1e-132,
Organism=Escherichia coli, GI1786733, Length=355, Percent_Identity=24.7887323943662, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324702, Length=383, Percent_Identity=22.7154046997389, Blast_Score=91, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: 2.1.2.- [C]

Molecular weight: Translated: 43083; Mature: 43083

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIGQRLGTPLSPSATRVMLLGAGELGKEVVIALQRLGVEVIAVDRYPDAPGHQVAHRAH
CCCCHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHEE
VIDMTDGAALRALVEAERPHLIVPEIEAIATDALAQIEAAGLAEVIPTARATQLTMNREG
EEECCCCHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHCCHHHHHCCCCCEEEEECHHH
IRRLAAETLGLPTSPYAFAESFDEFAAAVAQIGMPCVVKPVMSSSGKGQSVVRTEADVKP
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHHCCCCCCCEEEEECCCCCC
AWDYAMAGGRVNHGRVIVEGFIDFEYEITQLTVRAIDPATLDTRTWFCEPIGHVQVAGDY
CHHHHHCCCCCCCCEEEEEEEECCEEEEEEEEEEECCCCCCCCCEEEECCCCCEEEECHH
VESWQPQPMRAAALEKSREIAHKVTEALGGRGLFGVELFVRGDEVWFSEVSPRPHDTGLV
HHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEEEECCCCCCCCCCEE
TLASQRQSEFELHARAILGLPVDPTLGSPAASAVIYGGLDARGIAFEGVRDALAVPGADL
EEECCCCHHHHHEEHEEECCCCCCCCCCCHHCEEEECCCCCCCEEHHHHHHHHHCCCCCE
RLFGKPESFAKRRMGVALATGANVDEARERAKRAAAAVRPVAGN
EEECCCHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MQIGQRLGTPLSPSATRVMLLGAGELGKEVVIALQRLGVEVIAVDRYPDAPGHQVAHRAH
CCCCHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHEE
VIDMTDGAALRALVEAERPHLIVPEIEAIATDALAQIEAAGLAEVIPTARATQLTMNREG
EEECCCCHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHCCHHHHHCCCCCEEEEECHHH
IRRLAAETLGLPTSPYAFAESFDEFAAAVAQIGMPCVVKPVMSSSGKGQSVVRTEADVKP
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHHCCCCCCCEEEEECCCCCC
AWDYAMAGGRVNHGRVIVEGFIDFEYEITQLTVRAIDPATLDTRTWFCEPIGHVQVAGDY
CHHHHHCCCCCCCCEEEEEEEECCEEEEEEEEEEECCCCCCCCCEEEECCCCCEEEECHH
VESWQPQPMRAAALEKSREIAHKVTEALGGRGLFGVELFVRGDEVWFSEVSPRPHDTGLV
HHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEEEECCCCCCCCCCEE
TLASQRQSEFELHARAILGLPVDPTLGSPAASAVIYGGLDARGIAFEGVRDALAVPGADL
EEECCCCHHHHHEEHEEECCCCCCCCCCCHHCEEEECCCCCCCEEHHHHHHHHHCCCCCE
RLFGKPESFAKRRMGVALATGANVDEARERAKRAAAAVRPVAGN
EEECCCHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA