Click here to switch to the map view.

The map label for this gene is argT [H]

Identifier: 161524065

GI number: 161524065

Start: 971300

End: 972076

Strand: Reverse

Name: argT [H]

Synonym: Bmul_0886

Alternate gene names: 161524065

Gene position: 972076-971300 (Counterclockwise)

Preceding gene: 161524067

Following gene: 161524064

Centisome position: 28.19

GC content: 64.86

Gene sequence:

>777_bases
ATGAAGAAGGCCGCCCTGTGCGCCGCCCTCGCCCTCGTGGCGGGCAGCGCCTTCGCGAAGGAGTGGAAGACCGTGCGGAT
CGGCGTCGACGCGAGCTACCCGCCGTTCGAATCGACCGCGCCGAGCGGCGAGATCGTCGGTTTCGACGTCGATCTCACGA
AGGAAATCTGCAAGCGCATCGGCGTCAAGTGCGTATGGGTCGCGCAGGATCTCGACGGCATCATCCCGGCGCTGAAGGCG
AAGAAGTACGACATCATCGTGTCGTCGCTGACGGTCACCGACAAGCGCCGCGAGCAAATCGACTTTTCCGACAAGGTGTA
CGACGCGCCGGCGCGGATGATCGCGAAGGCCGGCTCGCCGCTGCAGCCGACGGTCGCGTCGCTGAAGGGCAAGCGCGTCG
GCGTCGAGCAGGGCTCGACGCAGGAAACCTACGCGAAGACGTACTGGGAACCGCAGGGCGTCACGATCATTCCTTACCAG
AACCAGGACCAGGTCTATGCCGATCTCGGCTCGGGCCGCCTCGACGCGACGCTGCAGGACGAACTGCAGGCCGACTACGG
CTTCCTGCGCACGCCGCGCGGCAAGGGTTTCGCGTTCGCGGGCCCCGAGGTGAAGGATCCGAAGACGATCGGCGACGGCA
CCGCAATCGGGCTGCGCAAGGAAGATACGGACCTGAAGATCCGCATCAACAAGGCGCTGGCCGACATGCACAAGGACGGC
ACGTACGACCGTCTGTCGCACAAGTATTTCTCGTTCAGCGTCTACTCGGCGCGCTGA

Upstream 100 bases:

>100_bases
AAAGCGCCGAACGGCGAATTTTGGCCGTCTAGAATACGCCGCGACGCCCGGCCCGACCGGCCGCGTGCGCGCCCGGAACG
TCAGCAACAGAAGGATGGAA

Downstream 100 bases:

>100_bases
GCGCCGCCACGGGACGCAGCGGACGCGGGGGCGGCCCCGCGGCCGCTCAGTACAGGCAGTCAACCACGCGCCGCCCGCCC
CTCGCCCGCCGCTTGCGTCG

Product: cationic amino acid ABC transporter periplasmic binding protein

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: LAO-binding protein [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MKKAALCAALALVAGSAFAKEWKTVRIGVDASYPPFESTAPSGEIVGFDVDLTKEICKRIGVKCVWVAQDLDGIIPALKA
KKYDIIVSSLTVTDKRREQIDFSDKVYDAPARMIAKAGSPLQPTVASLKGKRVGVEQGSTQETYAKTYWEPQGVTIIPYQ
NQDQVYADLGSGRLDATLQDELQADYGFLRTPRGKGFAFAGPEVKDPKTIGDGTAIGLRKEDTDLKIRINKALADMHKDG
TYDRLSHKYFSFSVYSAR

Sequences:

>Translated_258_residues
MKKAALCAALALVAGSAFAKEWKTVRIGVDASYPPFESTAPSGEIVGFDVDLTKEICKRIGVKCVWVAQDLDGIIPALKA
KKYDIIVSSLTVTDKRREQIDFSDKVYDAPARMIAKAGSPLQPTVASLKGKRVGVEQGSTQETYAKTYWEPQGVTIIPYQ
NQDQVYADLGSGRLDATLQDELQADYGFLRTPRGKGFAFAGPEVKDPKTIGDGTAIGLRKEDTDLKIRINKALADMHKDG
TYDRLSHKYFSFSVYSAR
>Mature_258_residues
MKKAALCAALALVAGSAFAKEWKTVRIGVDASYPPFESTAPSGEIVGFDVDLTKEICKRIGVKCVWVAQDLDGIIPALKA
KKYDIIVSSLTVTDKRREQIDFSDKVYDAPARMIAKAGSPLQPTVASLKGKRVGVEQGSTQETYAKTYWEPQGVTIIPYQ
NQDQVYADLGSGRLDATLQDELQADYGFLRTPRGKGFAFAGPEVKDPKTIGDGTAIGLRKEDTDLKIRINKALADMHKDG
TYDRLSHKYFSFSVYSAR

Specific function: This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=258, Percent_Identity=56.5891472868217, Blast_Score=305, Evalue=2e-84,
Organism=Escherichia coli, GI1788648, Length=233, Percent_Identity=54.5064377682403, Blast_Score=283, Evalue=1e-77,
Organism=Escherichia coli, GI1787085, Length=251, Percent_Identity=36.6533864541833, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1787088, Length=250, Percent_Identity=34.4, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1788228, Length=263, Percent_Identity=31.9391634980989, Blast_Score=120, Evalue=6e-29,
Organism=Escherichia coli, GI1787031, Length=248, Percent_Identity=31.8548387096774, Blast_Score=111, Evalue=4e-26,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28279; Mature: 28279

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKAALCAALALVAGSAFAKEWKTVRIGVDASYPPFESTAPSGEIVGFDVDLTKEICKRI
CCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHH
GVKCVWVAQDLDGIIPALKAKKYDIIVSSLTVTDKRREQIDFSDKVYDAPARMIAKAGSP
CCEEEEEECCHHHHHHHHCCCCEEEEEEEEEECCCHHHHCCCCCHHHHHHHHHHHHCCCC
LQPTVASLKGKRVGVEQGSTQETYAKTYWEPQGVTIIPYQNQDQVYADLGSGRLDATLQD
CCCHHHHCCCCEECCCCCCCHHHHHHHEECCCCEEEEEECCCCCEEEECCCCCCCCHHHH
ELQADYGFLRTPRGKGFAFAGPEVKDPKTIGDGTAIGLRKEDTDLKIRINKALADMHKDG
HHHHCCCEEECCCCCCEEECCCCCCCCCCCCCCCEEEEEECCCCEEEEEHHHHHHHHCCC
TYDRLSHKYFSFSVYSAR
CHHHHCCEEEEEEEEECC
>Mature Secondary Structure
MKKAALCAALALVAGSAFAKEWKTVRIGVDASYPPFESTAPSGEIVGFDVDLTKEICKRI
CCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHH
GVKCVWVAQDLDGIIPALKAKKYDIIVSSLTVTDKRREQIDFSDKVYDAPARMIAKAGSP
CCEEEEEECCHHHHHHHHCCCCEEEEEEEEEECCCHHHHCCCCCHHHHHHHHHHHHCCCC
LQPTVASLKGKRVGVEQGSTQETYAKTYWEPQGVTIIPYQNQDQVYADLGSGRLDATLQD
CCCHHHHCCCCEECCCCCCCHHHHHHHEECCCCEEEEEECCCCCEEEECCCCCCCCHHHH
ELQADYGFLRTPRGKGFAFAGPEVKDPKTIGDGTAIGLRKEDTDLKIRINKALADMHKDG
HHHHCCCEEECCCCCCEEECCCCCCCCCCCCCCCEEEEEECCCCEEEEEHHHHHHHHCCC
TYDRLSHKYFSFSVYSAR
CHHHHCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3040734; 9298646 [H]