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The map label for this gene is nadE2 [H]

Identifier: 161524057

GI number: 161524057

Start: 963367

End: 965067

Strand: Direct

Name: nadE2 [H]

Synonym: Bmul_0878

Alternate gene names: 161524057

Gene position: 963367-965067 (Clockwise)

Preceding gene: 161524054

Following gene: 161524058

Centisome position: 27.94

GC content: 69.19

Gene sequence:

>1701_bases
ATGAAGACCCGACTCGCTCTCGCCCAGATCAACGTCACCGTCGGCGACTTTGCCGGCAACGTCGCGCGCATCGTCGCGGC
GGCCCGTGCCGCGCACGAAGATGGTGCGCACCTGATGATCGCGCCGGAACTCGCGCTGTCCGGCTATCCGCCGGAAGACC
TGCTGCTGCGCCCCGCGTTCTACGCGGAAGCGGCCGCCGCGCTCGACGCGCTCGCCGATGCGCTGAAGCCGTTCGACGGG
CTCGCGGTGCTGGTCGGTCATCCGCTGCGCGGTGCGCCAGGCGTCGATGGTAATGCAAACCGCCCGATCGAGCGCGGTGT
GCCGCCGGTCGACACGTACAACGCGGCGTCGCTGATCGTCGGCGGCCGGATCGTCGGCACGTACCGCAAGCAGGATCTGC
CGAACGCCGAGGTGTTCGACGAGAAGCGCTATTTCGCGACCGACGCCGAACCGCTCGTCTTCGAGCTCAACGGCGTGAGA
TTCGGCGTGATCATCTGCGAGGATGCGTGGCACGCGTCGGCCGCGCAGATCGCCAAGGCGGCCGGCGCGCAGGTGCTGCT
GATCCCGAACGGCTCGCCGTACCACATGAACAAGGAAGCGGTGCGCGTCGACATTCTGCGCGCGCGCATTCGCGAGACGG
GGCTGCCGATGGTGTACGTGAACCTCGTCGGCGGGCAGGACGAGCTCGTGTTCGACGGCGGCTCGTTCGTGCTCGACGGG
CAGGGCGCGCTCGTCGCGCAGATGCCGCAGTTCGACGAAGGGCACGCGATCGTCGAATTCGACGGCGCGCGGCCGCTGCC
CGGCGCGATCGCGCCGCCGCTGCCGGTCGACGCGCAGGTCTATCGCGCGCTCGTGCTCGGCGTGCGCGACTACATCGGCA
AGAACGGCTTTCCGGGCGCGCTGATCGGGCTGTCGGGCGGCGTCGATTCGGCGCTCGTGCTGGCCGTCGCCTGCGACGCG
CTCGGGCCCGAGCGCGTGCGCGCCGTGATGATGCCGTCGCGCTACACGGCCGACATCTCGACGACGGACGCGGCCGACAT
GGCACGGCGCGTCGGCGTGCGCTACGACGAGATCGCGATCGCGCCGATGTTCGACGCGTTCCGGGCGGCGCTGGCCGCCG
AATTCGCGGGCCGCGCGGAAGACGCGACCGAGGAGAACATCCAGGCGCGCATCCGCGGCACGCTCTTGATGGCGCTGTCG
AACAAGTTCGGCTCGATCGTGCTCACGACTGGCAACAAGAGCGAGATGGCGGTCGGCTACTGCACGCTGTACGGCGACAT
GGCGGGCGGCTTCGCGGTGCTGAAGGACATCGCGAAAACGCTGGTCTACCGGCTGTGCCGCTACCGGAACGCGACGTCCG
ACTACGGGCGGCGCGACATCATCCCCGAACGGATCCTCACGCGCGCGCCGTCGGCCGAGTTGCGCGAGAACCAGACCGAT
CAGGACAGCCTGCCGCCGTACGACGTGCTCGACGCGATCATGCGGATGTACATGGAAGAGGACCGCCCGCTCGCCGAGAT
CGTCGCGGCCGGCTACGCGCCGGCCGACGTCGCGCGTGTCACGCGGCTCATCAAGCTCAACGAGTACAAGCGGCGTCAGG
CGCCGATCGGGATTCGCGTCACGCACCGTGCGTTCGGGCGCGACTGGCGTTATCCGATCACGTCGCGTTTCACCGAACGC
CTCGGCGAACGCCTTGACTGA

Upstream 100 bases:

>100_bases
GCGTGAATCCGTACCGTTTGGATGTCGCCGGTTGCGTGCGCGCCCCGCGACGTTTTCGTCAGCCGTTCACCGCGCGCCGC
CCGGCTGCGCTGCTCCCGTC

Downstream 100 bases:

>100_bases
GCCGCGCATGACGCCGGCGGCTTACAATCGGGATCGCGATTTTTCATTCCACACGAGCATTGAGGGACAACCATCATGAA
ACGCATCACTGCCATCATCA

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 566; Mature: 566

Protein sequence:

>566_residues
MKTRLALAQINVTVGDFAGNVARIVAAARAAHEDGAHLMIAPELALSGYPPEDLLLRPAFYAEAAAALDALADALKPFDG
LAVLVGHPLRGAPGVDGNANRPIERGVPPVDTYNAASLIVGGRIVGTYRKQDLPNAEVFDEKRYFATDAEPLVFELNGVR
FGVIICEDAWHASAAQIAKAAGAQVLLIPNGSPYHMNKEAVRVDILRARIRETGLPMVYVNLVGGQDELVFDGGSFVLDG
QGALVAQMPQFDEGHAIVEFDGARPLPGAIAPPLPVDAQVYRALVLGVRDYIGKNGFPGALIGLSGGVDSALVLAVACDA
LGPERVRAVMMPSRYTADISTTDAADMARRVGVRYDEIAIAPMFDAFRAALAAEFAGRAEDATEENIQARIRGTLLMALS
NKFGSIVLTTGNKSEMAVGYCTLYGDMAGGFAVLKDIAKTLVYRLCRYRNATSDYGRRDIIPERILTRAPSAELRENQTD
QDSLPPYDVLDAIMRMYMEEDRPLAEIVAAGYAPADVARVTRLIKLNEYKRRQAPIGIRVTHRAFGRDWRYPITSRFTER
LGERLD

Sequences:

>Translated_566_residues
MKTRLALAQINVTVGDFAGNVARIVAAARAAHEDGAHLMIAPELALSGYPPEDLLLRPAFYAEAAAALDALADALKPFDG
LAVLVGHPLRGAPGVDGNANRPIERGVPPVDTYNAASLIVGGRIVGTYRKQDLPNAEVFDEKRYFATDAEPLVFELNGVR
FGVIICEDAWHASAAQIAKAAGAQVLLIPNGSPYHMNKEAVRVDILRARIRETGLPMVYVNLVGGQDELVFDGGSFVLDG
QGALVAQMPQFDEGHAIVEFDGARPLPGAIAPPLPVDAQVYRALVLGVRDYIGKNGFPGALIGLSGGVDSALVLAVACDA
LGPERVRAVMMPSRYTADISTTDAADMARRVGVRYDEIAIAPMFDAFRAALAAEFAGRAEDATEENIQARIRGTLLMALS
NKFGSIVLTTGNKSEMAVGYCTLYGDMAGGFAVLKDIAKTLVYRLCRYRNATSDYGRRDIIPERILTRAPSAELRENQTD
QDSLPPYDVLDAIMRMYMEEDRPLAEIVAAGYAPADVARVTRLIKLNEYKRRQAPIGIRVTHRAFGRDWRYPITSRFTER
LGERLD
>Mature_566_residues
MKTRLALAQINVTVGDFAGNVARIVAAARAAHEDGAHLMIAPELALSGYPPEDLLLRPAFYAEAAAALDALADALKPFDG
LAVLVGHPLRGAPGVDGNANRPIERGVPPVDTYNAASLIVGGRIVGTYRKQDLPNAEVFDEKRYFATDAEPLVFELNGVR
FGVIICEDAWHASAAQIAKAAGAQVLLIPNGSPYHMNKEAVRVDILRARIRETGLPMVYVNLVGGQDELVFDGGSFVLDG
QGALVAQMPQFDEGHAIVEFDGARPLPGAIAPPLPVDAQVYRALVLGVRDYIGKNGFPGALIGLSGGVDSALVLAVACDA
LGPERVRAVMMPSRYTADISTTDAADMARRVGVRYDEIAIAPMFDAFRAALAAEFAGRAEDATEENIQARIRGTLLMALS
NKFGSIVLTTGNKSEMAVGYCTLYGDMAGGFAVLKDIAKTLVYRLCRYRNATSDYGRRDIIPERILTRAPSAELRENQTD
QDSLPPYDVLDAIMRMYMEEDRPLAEIVAAGYAPADVARVTRLIKLNEYKRRQAPIGIRVTHRAFGRDWRYPITSRFTER
LGERLD

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR000132
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 61225; Mature: 61225

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTRLALAQINVTVGDFAGNVARIVAAARAAHEDGAHLMIAPELALSGYPPEDLLLRPAF
CCCEEEEEEEEEEEHHHCCHHHHHHHHHHHHCCCCCEEEEECHHHCCCCCCHHHEECCHH
YAEAAAALDALADALKPFDGLAVLVGHPLRGAPGVDGNANRPIERGVPPVDTYNAASLIV
HHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCEEEE
GGRIVGTYRKQDLPNAEVFDEKRYFATDAEPLVFELNGVRFGVIICEDAWHASAAQIAKA
CCEEEEEHHHCCCCCCHHHCCCCEEECCCCCEEEEECCEEEEEEEECCCCCCHHHHHHHH
AGAQVLLIPNGSPYHMNKEAVRVDILRARIRETGLPMVYVNLVGGQDELVFDGGSFVLDG
CCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCEEECC
QGALVAQMPQFDEGHAIVEFDGARPLPGAIAPPLPVDAQVYRALVLGVRDYIGKNGFPGA
CCCEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCE
LIGLSGGVDSALVLAVACDALGPERVRAVMMPSRYTADISTTDAADMARRVGVRYDEIAI
EEEECCCCCHHHHHHHHHHCCCHHHHHEEECCCCEECCCCCCCHHHHHHHHCCCCHHEEE
APMFDAFRAALAAEFAGRAEDATEENIQARIRGTLLMALSNKFGSIVLTTGNKSEMAVGY
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEEEECCCCCEEEEEECCCCCEEEEE
CTLYGDMAGGFAVLKDIAKTLVYRLCRYRNATSDYGRRDIIPERILTRAPSAELRENQTD
EEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCHHHHCCCCC
QDSLPPYDVLDAIMRMYMEEDRPLAEIVAAGYAPADVARVTRLIKLNEYKRRQAPIGIRV
CCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEE
THRAFGRDWRYPITSRFTERLGERLD
EHHHHCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKTRLALAQINVTVGDFAGNVARIVAAARAAHEDGAHLMIAPELALSGYPPEDLLLRPAF
CCCEEEEEEEEEEEHHHCCHHHHHHHHHHHHCCCCCEEEEECHHHCCCCCCHHHEECCHH
YAEAAAALDALADALKPFDGLAVLVGHPLRGAPGVDGNANRPIERGVPPVDTYNAASLIV
HHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCEEEE
GGRIVGTYRKQDLPNAEVFDEKRYFATDAEPLVFELNGVRFGVIICEDAWHASAAQIAKA
CCEEEEEHHHCCCCCCHHHCCCCEEECCCCCEEEEECCEEEEEEEECCCCCCHHHHHHHH
AGAQVLLIPNGSPYHMNKEAVRVDILRARIRETGLPMVYVNLVGGQDELVFDGGSFVLDG
CCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCEEECC
QGALVAQMPQFDEGHAIVEFDGARPLPGAIAPPLPVDAQVYRALVLGVRDYIGKNGFPGA
CCCEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCE
LIGLSGGVDSALVLAVACDALGPERVRAVMMPSRYTADISTTDAADMARRVGVRYDEIAI
EEEECCCCCHHHHHHHHHHCCCHHHHHEEECCCCEECCCCCCCHHHHHHHHCCCCHHEEE
APMFDAFRAALAAEFAGRAEDATEENIQARIRGTLLMALSNKFGSIVLTTGNKSEMAVGY
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEEEECCCCCEEEEEECCCCCEEEEE
CTLYGDMAGGFAVLKDIAKTLVYRLCRYRNATSDYGRRDIIPERILTRAPSAELRENQTD
EEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCHHHHCCCCC
QDSLPPYDVLDAIMRMYMEEDRPLAEIVAAGYAPADVARVTRLIKLNEYKRRQAPIGIRV
CCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEE
THRAFGRDWRYPITSRFTERLGERLD
EHHHHCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]