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The map label for this gene is metG

Identifier: 161524036

GI number: 161524036

Start: 935532

End: 937700

Strand: Reverse

Name: metG

Synonym: Bmul_0857

Alternate gene names: 161524036

Gene position: 937700-935532 (Counterclockwise)

Preceding gene: 161524037

Following gene: 161524033

Centisome position: 27.19

GC content: 65.84

Gene sequence:

>2169_bases
ATGTCCGCATCCGACCTCACTTCCGTGCAGGCCGCGGCGCCGCAAGGCCGCCGCCAGATCCTCGTCACGTCCGCCCTGCC
CTACGCCAACGGCCAGATCCATATCGGCCATCTGGTCGAGTACATCCAGACCGACATCTGGGTGCGGACGCTGCGAATGC
ACGGGCACGAGGTCTACTACATCGGCGCCGACGACACGCACGGCACGCCGGTCATGCTGCGCGCGGAGAAGGAAGGCCTG
ACGCCGAAGCAGCTGATCGAGCGCGTATGGGCCGAGCACAAGCGCGACTTCGACAGCTTCGGCGTGTCGTTCGACAACTT
CTACTCGACCGATTCGGAAGAAAACCGCCAGCTGAGCGAGAAGATCTACCTCGCGCTGAAGGACAACGGGCTGATCGCCG
AGCGCGAGATCGAGCAGGCGTACGACCCGGTCAAGGAGATGTTCCTGCCGGACCGTTTCATCAAGGGCGAGTGCCCGAAG
TGCCACGCGAAGGACCAGTACGGCGACAGCTGCGAAGTGTGCGGCTCGACCTACCTGCCGACCGAGCTGCTGAACCCGTA
TTCGGTCGTGTCGGGCGCGACGCCCGTGCGCAAGACGTCGAAGCACTACTTCTTCCGCCTGTCCGACCCGCGCTGCGAGA
CGTTCCTGCGCGAATGGGTGAGCGGCCTCGCGCAGCCCGAAGCGACCAACAAGATGCGCGAATGGCTCGGCGACGCCGGC
GAAGCCAAGCTCGCCGACTGGGACATCTCGCGCGACGCACCGTATTTCGGCTTCGAGATCCCCGGCGCGCCGGGCAAGTA
CTTCTACGTGTGGCTCGATGCGCCGGTCGGCTACTACGCGAGCTTCAAGAATCTGTGCGATCGCAAAGGCATCGACTTCG
ATGCGTGGATCCGCGCCGGTTCGAGCGCCGAGCAGTACCACTTCATCGGCAAGGACATCCTGTATTTCCACACGCTGTTC
TGGCCGGCGATGCTGCAGTTCTCGGGCCACCGCACGCCGACCAACGTGTTCGCGCACGGCTTCCTGACCGTCGACGGCGC
GAAGATGTCGAAGTCGCGCGGCACGTTCATTACCGCGCAGAGCTATATCGACACCGGCCTGAACCCCGAATGGCTGCGCT
ACTACTTCGCCGCGAAGCTGAACGCGACGATGGAAGACATCGATCTGAACCTCGACGACTTCCAGGCGCGCGTGAATAGC
GACCTCGTCGGCAAGTACGTGAACATCGCGAGCCGCGCGGCGGGCTTTCTGATCAAGCGCTTCGACGGCCGCGTGCAGGA
CAGCGCGATGAACCATCCGCTCGTCGCGAAGCTGCGCGAAGCGATTCCGCAGATCGCCGCGCACTACGAGGCGCGCGAGT
ACGGCCGCGCGTTGCGCCACACGATGGAACTCGCCGACGAAGTGAACGCCTACGTCGACGGCGCCAAGCCGTGGGAGCTC
GCGAAGGATCCGGCGAACGCGGTCGCGCTGCACGAAACCTGCAGCGTGAGCCTCGAGGCGTTCCGCCTGCTGTCGCTCGC
GCTCAAGCCCGTGCTGCCGCGCGTGGCCGAAGCGGTCGAGGCGTTCTTCGGCATCGCGCCGCTCGCGTGGGCCGATGCCG
CGAAGCCGCTGTCGTCCGCGCAGCCGATCAAGGCCTACCAGCACCTGATGACGCGCGTCGACGCGAAGCAGATCGAAGCG
CTGCTCGCCGCCAACCGCGATTCGCTGCAGGCCGACGCAACGGCCGCCGGCGGCGCGGGCGCCGCGACGGCGAAGGGTGC
GAAAGCCGCGAAGGCCGATGCGAAGCCGGTCAGCGCGAAAGCGCCCGCCGCGAACGGTGCCGACGACGCAACGATTTCGA
TCGACGATTTCGCGAAGATCGATCTGCGCATCGCGAAGATCGTCGCGTGCCAGGCCGTCGAAGGGTCGGACAAGCTGCTG
CAGCTGACGCTCGACGTCGGCGAGGAGAAGACGCGCAACGTGTTCTCGGGGATCAAGTCCGCGTATCAGCCCGAGCAGCT
CGTCGGCAAGCTGACGGTCATGGTCGCGAACCTCGCGCCGCGCAAGATGAAGTTCGGGCTGTCCGAAGGGATGGTGCTCG
CGGCCTCGGCGACCGACGAGAAGGCCGAACCGGGGCTCTATATCCTCGAGCCGCACAGCGGCGCGAAGCCCGGCATGCGC
GTGAAGTAA

Upstream 100 bases:

>100_bases
CGGCTGCCGAACCGCCGCGCGCCGGCGCCGCGCCTGCCCGGTCCGCGCCCCTGCTAGAATCGCAGTTTCCGTCGTTTTCC
GCTGTTCCTCTACAGACCCT

Downstream 100 bases:

>100_bases
GCAGCGCGCCTCGCCCGCCCGAACCGCCCCGCACCGCTCGTGCGGGGCTTTTTTTCGTCGGTCGGCAGCGCGGGCCGGCG
AGCGTGTTCTCAGCGCCGCC

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 722; Mature: 721

Protein sequence:

>722_residues
MSASDLTSVQAAAPQGRRQILVTSALPYANGQIHIGHLVEYIQTDIWVRTLRMHGHEVYYIGADDTHGTPVMLRAEKEGL
TPKQLIERVWAEHKRDFDSFGVSFDNFYSTDSEENRQLSEKIYLALKDNGLIAEREIEQAYDPVKEMFLPDRFIKGECPK
CHAKDQYGDSCEVCGSTYLPTELLNPYSVVSGATPVRKTSKHYFFRLSDPRCETFLREWVSGLAQPEATNKMREWLGDAG
EAKLADWDISRDAPYFGFEIPGAPGKYFYVWLDAPVGYYASFKNLCDRKGIDFDAWIRAGSSAEQYHFIGKDILYFHTLF
WPAMLQFSGHRTPTNVFAHGFLTVDGAKMSKSRGTFITAQSYIDTGLNPEWLRYYFAAKLNATMEDIDLNLDDFQARVNS
DLVGKYVNIASRAAGFLIKRFDGRVQDSAMNHPLVAKLREAIPQIAAHYEAREYGRALRHTMELADEVNAYVDGAKPWEL
AKDPANAVALHETCSVSLEAFRLLSLALKPVLPRVAEAVEAFFGIAPLAWADAAKPLSSAQPIKAYQHLMTRVDAKQIEA
LLAANRDSLQADATAAGGAGAATAKGAKAAKADAKPVSAKAPAANGADDATISIDDFAKIDLRIAKIVACQAVEGSDKLL
QLTLDVGEEKTRNVFSGIKSAYQPEQLVGKLTVMVANLAPRKMKFGLSEGMVLAASATDEKAEPGLYILEPHSGAKPGMR
VK

Sequences:

>Translated_722_residues
MSASDLTSVQAAAPQGRRQILVTSALPYANGQIHIGHLVEYIQTDIWVRTLRMHGHEVYYIGADDTHGTPVMLRAEKEGL
TPKQLIERVWAEHKRDFDSFGVSFDNFYSTDSEENRQLSEKIYLALKDNGLIAEREIEQAYDPVKEMFLPDRFIKGECPK
CHAKDQYGDSCEVCGSTYLPTELLNPYSVVSGATPVRKTSKHYFFRLSDPRCETFLREWVSGLAQPEATNKMREWLGDAG
EAKLADWDISRDAPYFGFEIPGAPGKYFYVWLDAPVGYYASFKNLCDRKGIDFDAWIRAGSSAEQYHFIGKDILYFHTLF
WPAMLQFSGHRTPTNVFAHGFLTVDGAKMSKSRGTFITAQSYIDTGLNPEWLRYYFAAKLNATMEDIDLNLDDFQARVNS
DLVGKYVNIASRAAGFLIKRFDGRVQDSAMNHPLVAKLREAIPQIAAHYEAREYGRALRHTMELADEVNAYVDGAKPWEL
AKDPANAVALHETCSVSLEAFRLLSLALKPVLPRVAEAVEAFFGIAPLAWADAAKPLSSAQPIKAYQHLMTRVDAKQIEA
LLAANRDSLQADATAAGGAGAATAKGAKAAKADAKPVSAKAPAANGADDATISIDDFAKIDLRIAKIVACQAVEGSDKLL
QLTLDVGEEKTRNVFSGIKSAYQPEQLVGKLTVMVANLAPRKMKFGLSEGMVLAASATDEKAEPGLYILEPHSGAKPGMR
VK
>Mature_721_residues
SASDLTSVQAAAPQGRRQILVTSALPYANGQIHIGHLVEYIQTDIWVRTLRMHGHEVYYIGADDTHGTPVMLRAEKEGLT
PKQLIERVWAEHKRDFDSFGVSFDNFYSTDSEENRQLSEKIYLALKDNGLIAEREIEQAYDPVKEMFLPDRFIKGECPKC
HAKDQYGDSCEVCGSTYLPTELLNPYSVVSGATPVRKTSKHYFFRLSDPRCETFLREWVSGLAQPEATNKMREWLGDAGE
AKLADWDISRDAPYFGFEIPGAPGKYFYVWLDAPVGYYASFKNLCDRKGIDFDAWIRAGSSAEQYHFIGKDILYFHTLFW
PAMLQFSGHRTPTNVFAHGFLTVDGAKMSKSRGTFITAQSYIDTGLNPEWLRYYFAAKLNATMEDIDLNLDDFQARVNSD
LVGKYVNIASRAAGFLIKRFDGRVQDSAMNHPLVAKLREAIPQIAAHYEAREYGRALRHTMELADEVNAYVDGAKPWELA
KDPANAVALHETCSVSLEAFRLLSLALKPVLPRVAEAVEAFFGIAPLAWADAAKPLSSAQPIKAYQHLMTRVDAKQIEAL
LAANRDSLQADATAAGGAGAATAKGAKAAKADAKPVSAKAPAANGADDATISIDDFAKIDLRIAKIVACQAVEGSDKLLQ
LTLDVGEEKTRNVFSGIKSAYQPEQLVGKLTVMVANLAPRKMKFGLSEGMVLAASATDEKAEPGLYILEPHSGAKPGMRV
K

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI14043022, Length=564, Percent_Identity=27.3049645390071, Blast_Score=218, Evalue=2e-56,
Organism=Homo sapiens, GI24308436, Length=538, Percent_Identity=26.9516728624535, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI4507947, Length=145, Percent_Identity=35.1724137931034, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1788432, Length=715, Percent_Identity=53.006993006993, Blast_Score=751, Evalue=0.0,
Organism=Escherichia coli, GI1789455, Length=111, Percent_Identity=36.9369369369369, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17540794, Length=678, Percent_Identity=25.9587020648968, Blast_Score=229, Evalue=4e-60,
Organism=Caenorhabditis elegans, GI212645096, Length=542, Percent_Identity=24.169741697417, Blast_Score=139, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI71992781, Length=432, Percent_Identity=23.3796296296296, Blast_Score=102, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6321704, Length=563, Percent_Identity=27.886323268206, Blast_Score=230, Evalue=8e-61,
Organism=Saccharomyces cerevisiae, GI6321610, Length=544, Percent_Identity=24.6323529411765, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24655311, Length=523, Percent_Identity=29.0630975143403, Blast_Score=219, Evalue=5e-57,
Organism=Drosophila melanogaster, GI21358483, Length=552, Percent_Identity=26.8115942028986, Blast_Score=132, Evalue=8e-31,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_BURM1 (A9AHJ8)

Other databases:

- EMBL:   CP000868
- EMBL:   AP009385
- RefSeq:   YP_001579048.1
- RefSeq:   YP_001946831.1
- ProteinModelPortal:   A9AHJ8
- SMR:   A9AHJ8
- GeneID:   5766674
- GeneID:   6358528
- GenomeReviews:   AP009385_GR
- GenomeReviews:   CP000868_GR
- KEGG:   bmj:BMULJ_02401
- KEGG:   bmu:Bmul_0857
- HOGENOM:   HBG721667
- OMA:   SAYQPEQ
- ProtClustDB:   PRK00133
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 79632; Mature: 79501

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD

Important sites: BINDING 351-351

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASDLTSVQAAAPQGRRQILVTSALPYANGQIHIGHLVEYIQTDIWVRTLRMHGHEVYY
CCCCHHCHHHHCCCCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCEEEE
IGADDTHGTPVMLRAEKEGLTPKQLIERVWAEHKRDFDSFGVSFDNFYSTDSEENRQLSE
EECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHCCCHHHCCCCCCHHHHHHHH
KIYLALKDNGLIAEREIEQAYDPVKEMFLPDRFIKGECPKCHAKDQYGDSCEVCGSTYLP
EEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCHHHCCCCCCC
TELLNPYSVVSGATPVRKTSKHYFFRLSDPRCETFLREWVSGLAQPEATNKMREWLGDAG
HHHCCCHHHHCCCCCCHHCCCCEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
EAKLADWDISRDAPYFGFEIPGAPGKYFYVWLDAPVGYYASFKNLCDRKGIDFDAWIRAG
CCEEECCCCCCCCCCCCEECCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHCCC
SSAEQYHFIGKDILYFHTLFWPAMLQFSGHRTPTNVFAHGFLTVDGAKMSKSRGTFITAQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEEEEEEECCCCCCCCCCCEEEEH
SYIDTGLNPEWLRYYFAAKLNATMEDIDLNLDDFQARVNSDLVGKYVNIASRAAGFLIKR
HHHCCCCCHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
FDGRVQDSAMNHPLVAKLREAIPQIAAHYEAREYGRALRHTMELADEVNAYVDGAKPWEL
HCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
AKDPANAVALHETCSVSLEAFRLLSLALKPVLPRVAEAVEAFFGIAPLAWADAAKPLSSA
CCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCC
QPIKAYQHLMTRVDAKQIEALLAANRDSLQADATAAGGAGAATAKGAKAAKADAKPVSAK
CHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCC
APAANGADDATISIDDFAKIDLRIAKIVACQAVEGSDKLLQLTLDVGEEKTRNVFSGIKS
CCCCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHH
AYQPEQLVGKLTVMVANLAPRKMKFGLSEGMVLAASATDEKAEPGLYILEPHSGAKPGMR
CCCHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEEEECCCCCCCCCEEEECCCCCCCCCCC
VK
CC
>Mature Secondary Structure 
SASDLTSVQAAAPQGRRQILVTSALPYANGQIHIGHLVEYIQTDIWVRTLRMHGHEVYY
CCCHHCHHHHCCCCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCEEEE
IGADDTHGTPVMLRAEKEGLTPKQLIERVWAEHKRDFDSFGVSFDNFYSTDSEENRQLSE
EECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHCCCHHHCCCCCCHHHHHHHH
KIYLALKDNGLIAEREIEQAYDPVKEMFLPDRFIKGECPKCHAKDQYGDSCEVCGSTYLP
EEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCHHHCCCCCCC
TELLNPYSVVSGATPVRKTSKHYFFRLSDPRCETFLREWVSGLAQPEATNKMREWLGDAG
HHHCCCHHHHCCCCCCHHCCCCEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
EAKLADWDISRDAPYFGFEIPGAPGKYFYVWLDAPVGYYASFKNLCDRKGIDFDAWIRAG
CCEEECCCCCCCCCCCCEECCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHCCC
SSAEQYHFIGKDILYFHTLFWPAMLQFSGHRTPTNVFAHGFLTVDGAKMSKSRGTFITAQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEEEEEEECCCCCCCCCCCEEEEH
SYIDTGLNPEWLRYYFAAKLNATMEDIDLNLDDFQARVNSDLVGKYVNIASRAAGFLIKR
HHHCCCCCHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
FDGRVQDSAMNHPLVAKLREAIPQIAAHYEAREYGRALRHTMELADEVNAYVDGAKPWEL
HCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
AKDPANAVALHETCSVSLEAFRLLSLALKPVLPRVAEAVEAFFGIAPLAWADAAKPLSSA
CCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCC
QPIKAYQHLMTRVDAKQIEALLAANRDSLQADATAAGGAGAATAKGAKAAKADAKPVSAK
CHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCC
APAANGADDATISIDDFAKIDLRIAKIVACQAVEGSDKLLQLTLDVGEEKTRNVFSGIKS
CCCCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHH
AYQPEQLVGKLTVMVANLAPRKMKFGLSEGMVLAASATDEKAEPGLYILEPHSGAKPGMR
CCCHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEEEECCCCCCCCCEEEECCCCCCCCCCC
VK
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA