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The map label for this gene is braE [H]

Identifier: 161523978

GI number: 161523978

Start: 877840

End: 879009

Strand: Direct

Name: braE [H]

Synonym: Bmul_0798

Alternate gene names: 161523978

Gene position: 877840-879009 (Clockwise)

Preceding gene: 161523977

Following gene: 161523979

Centisome position: 25.46

GC content: 65.47

Gene sequence:

>1170_bases
ATGACATCCATTCAACCGATCGAATCGTCGACGACGCTCGTCGAAGAGCGCAACACCGCGAAGACCGTCACCATCGGCAT
CCTGACCGCGGCGTTCGTGATCGCCGCCCCGCTCGTGATCGGCGCGGCCGGCGGCAACTACTGGGTCCGCGTGCTCGATT
TCGCGATGCTCTACGTGATGCTCGCGCTCGGCCTGAACGTCGTGGTCGGCTTCGCCGGCCTGCTCGACCTCGGCTACATC
GCGTTCTACGCGGTGGGCGCCTATACGGCGGCGCTGCTCAGCTCGCCGCACCTGACCTCGCAGTTCGAGTGGATCGCGGC
GCTCGCGCCGAACGGGCTGCACGTGCCGTTCCTGATCATCGTGCCGATCGCGATGGCGCTCGCGGCGACGTTCGGCGTGC
TGCTCGGTGCGCCGACGCTGCGCCTGCGCGGCGACTATCTGGCGATCGTCACGCTCGGCTTCGGTGAAATCGTCCGGATC
TTCATGAACAACCTTGATCGTCCGGTCAACATCACGAACGGTCCGAAGGGGATCACGGGCATCGATCCGGTGCACGTCGG
CGGCTTCAACCTGTCGCAGACGCACACGCTGTTCGGCTTCCAGCTGCCGTCCGTGTACATGTACTACTACCTGTTCGTGC
TCTGCGCGCTGCTCGTGATCTGGGTCTGTACGCGTCTGCAGCACTCGCGCATCGGCCGCGCATGGGCCGCGATCCGCGAA
GACGAGATCGCCGCGAAGGCGATGGGTATCAACACACGCAACGTGAAGCTGCTCGCGTTCGCGATGGGCGCGTCGTTCGG
CGGGCTGTCGGGCGCGATGTTCGGCGCGTTCCAGGGCTTCGTGTCGCCGGAATCGTTCACGTTCTGGGAGTCGATCGTCG
TGCTCGCGTGCGTCGTGCTCGGCGGCATGGGCCATATCCCCGGCGTGATCCTCGGCGCGGTGCTGCTCGCGGTGTTCCCG
GAATTCCTGCGCTCGACGATGAGCCCGCTGCAGCACGCGCTGTTCGGCCACGACGTCGTCGATACCGAAGTGATCCGTCA
GGCGCTGTACGGCCTCGCGATGGTCATCATCATGCTGTATCGCTCGGAAGGCCTGTGGCCCGCGCCGAAGCATGAGGACA
AGATCGCGAAGCTGGCCAAGCGCAACGGCAAGAAGCCGGTGCGCGCGTAA

Upstream 100 bases:

>100_bases
GTTCGCATTCATCGTGCTGATCATCGTGCTGGTGTTCCGTCCGTCGGGCCTGCTGGGCGAACGGGTCGCAGACCGCGCGT
AACGGAAGGGAGCAAACAAC

Downstream 100 bases:

>100_bases
TCAACGGACAAGGGGATCCACACATGAGCGACAAGCAAATCCGACTGTCCGTGCAGGGCGTGAACAAGCGCTTCGGCGGG
CTGCAGGCACTGTCCGACGT

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MTSIQPIESSTTLVEERNTAKTVTIGILTAAFVIAAPLVIGAAGGNYWVRVLDFAMLYVMLALGLNVVVGFAGLLDLGYI
AFYAVGAYTAALLSSPHLTSQFEWIAALAPNGLHVPFLIIVPIAMALAATFGVLLGAPTLRLRGDYLAIVTLGFGEIVRI
FMNNLDRPVNITNGPKGITGIDPVHVGGFNLSQTHTLFGFQLPSVYMYYYLFVLCALLVIWVCTRLQHSRIGRAWAAIRE
DEIAAKAMGINTRNVKLLAFAMGASFGGLSGAMFGAFQGFVSPESFTFWESIVVLACVVLGGMGHIPGVILGAVLLAVFP
EFLRSTMSPLQHALFGHDVVDTEVIRQALYGLAMVIIMLYRSEGLWPAPKHEDKIAKLAKRNGKKPVRA

Sequences:

>Translated_389_residues
MTSIQPIESSTTLVEERNTAKTVTIGILTAAFVIAAPLVIGAAGGNYWVRVLDFAMLYVMLALGLNVVVGFAGLLDLGYI
AFYAVGAYTAALLSSPHLTSQFEWIAALAPNGLHVPFLIIVPIAMALAATFGVLLGAPTLRLRGDYLAIVTLGFGEIVRI
FMNNLDRPVNITNGPKGITGIDPVHVGGFNLSQTHTLFGFQLPSVYMYYYLFVLCALLVIWVCTRLQHSRIGRAWAAIRE
DEIAAKAMGINTRNVKLLAFAMGASFGGLSGAMFGAFQGFVSPESFTFWESIVVLACVVLGGMGHIPGVILGAVLLAVFP
EFLRSTMSPLQHALFGHDVVDTEVIRQALYGLAMVIIMLYRSEGLWPAPKHEDKIAKLAKRNGKKPVRA
>Mature_388_residues
TSIQPIESSTTLVEERNTAKTVTIGILTAAFVIAAPLVIGAAGGNYWVRVLDFAMLYVMLALGLNVVVGFAGLLDLGYIA
FYAVGAYTAALLSSPHLTSQFEWIAALAPNGLHVPFLIIVPIAMALAATFGVLLGAPTLRLRGDYLAIVTLGFGEIVRIF
MNNLDRPVNITNGPKGITGIDPVHVGGFNLSQTHTLFGFQLPSVYMYYYLFVLCALLVIWVCTRLQHSRIGRAWAAIRED
EIAAKAMGINTRNVKLLAFAMGASFGGLSGAMFGAFQGFVSPESFTFWESIVVLACVVLGGMGHIPGVILGAVLLAVFPE
FLRSTMSPLQHALFGHDVVDTEVIRQALYGLAMVIIMLYRSEGLWPAPKHEDKIAKLAKRNGKKPVRA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=345, Percent_Identity=42.8985507246377, Blast_Score=212, Evalue=3e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 41901; Mature: 41770

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSIQPIESSTTLVEERNTAKTVTIGILTAAFVIAAPLVIGAAGGNYWVRVLDFAMLYVM
CCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
LALGLNVVVGFAGLLDLGYIAFYAVGAYTAALLSSPHLTSQFEWIAALAPNGLHVPFLII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCHHHHH
VPIAMALAATFGVLLGAPTLRLRGDYLAIVTLGFGEIVRIFMNNLDRPVNITNGPKGITG
HHHHHHHHHHHHHHHCCCCEEECCCEEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCC
IDPVHVGGFNLSQTHTLFGFQLPSVYMYYYLFVLCALLVIWVCTRLQHSRIGRAWAAIRE
CCCCEECCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEIAAKAMGINTRNVKLLAFAMGASFGGLSGAMFGAFQGFVSPESFTFWESIVVLACVVL
HHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
GGMGHIPGVILGAVLLAVFPEFLRSTMSPLQHALFGHDVVDTEVIRQALYGLAMVIIMLY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
RSEGLWPAPKHEDKIAKLAKRNGKKPVRA
HCCCCCCCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TSIQPIESSTTLVEERNTAKTVTIGILTAAFVIAAPLVIGAAGGNYWVRVLDFAMLYVM
CCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
LALGLNVVVGFAGLLDLGYIAFYAVGAYTAALLSSPHLTSQFEWIAALAPNGLHVPFLII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCHHHHH
VPIAMALAATFGVLLGAPTLRLRGDYLAIVTLGFGEIVRIFMNNLDRPVNITNGPKGITG
HHHHHHHHHHHHHHHCCCCEEECCCEEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCC
IDPVHVGGFNLSQTHTLFGFQLPSVYMYYYLFVLCALLVIWVCTRLQHSRIGRAWAAIRE
CCCCEECCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEIAAKAMGINTRNVKLLAFAMGASFGGLSGAMFGAFQGFVSPESFTFWESIVVLACVVL
HHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
GGMGHIPGVILGAVLLAVFPEFLRSTMSPLQHALFGHDVVDTEVIRQALYGLAMVIIMLY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
RSEGLWPAPKHEDKIAKLAKRNGKKPVRA
HCCCCCCCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]