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The map label for this gene is purN [H]

Identifier: 161523965

GI number: 161523965

Start: 863863

End: 864525

Strand: Direct

Name: purN [H]

Synonym: Bmul_0785

Alternate gene names: 161523965

Gene position: 863863-864525 (Clockwise)

Preceding gene: 161523959

Following gene: 161523966

Centisome position: 25.05

GC content: 68.48

Gene sequence:

>663_bases
ATGAAAAAACTCGTGATCCTGATTTCCGGTCGCGGCAGCAACATGGAGGCCATCGTCCGCGCATGCACGCACGAACGCTG
GCCGGCCGAGGTCGCCGCCGTGATCGCCAACCGGCCCGACGCGGCCGGCCTCGCCTTTGCCGCGTCGCACGGCATTGCGA
CCGCGGTGGTCGACCATCGGTCCTTCGACGGCCGCGACAGCTTCGATGCGGCGCTCGCCGCCGAGATCGACCGCTTCGCG
CCCGATCTCGTCGTGCTCGCCGGCTTCATGCGTATCCTGACGCCTGAATTCGTCAGACGATATGAAGGCCGGCTGCTGAA
CATTCATCCGTCGCTGCTGCCGAGCTTCAAGGGCATCCGCACGCACCAGCAGGCGCTCGACGCGGGCGTCGCGCTGCACG
GCGCGACGGTTCATTTCGTCATTCCGGAACTCGACAGCGGCGCGATCGTCGCGCAGGGCGCGGTGCCGGTGCGCGCGGGC
GACGACGCCGCGGCGCTCGCGCAGCGCGTGCTGGCGGTCGAGCACGTGCTGTATCCGCGGGCGGTGCGCTGGTTCGTCGA
AGGGCGCCTGCGCCTCGAAGGCGACCGTGCGATCGTCGCGCCCGAAGAGGCGCGCTGGATTTTCGCGGATCAACCGCAAA
CCGAAACGAGCGAGGGTGTATGA

Upstream 100 bases:

>100_bases
GATGGGCGGTGGGATGCCGCAAAGCAGAAGATTATAAACGTTCGGGCGGCGCGGCGGCTGAGGAGGCGGGAAACGCGGGG
CCGAATGGTAAAATTCGCGG

Downstream 100 bases:

>100_bases
AGCTGCACGGATTCCTGATCGGCCAGACCGAGACGCTGCTGGCCGAAGTGCTGAAATTTGCCGGTCCCGCCGACGCGACG
ACGAGCCGCTTTTTCCGCGC

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MKKLVILISGRGSNMEAIVRACTHERWPAEVAAVIANRPDAAGLAFAASHGIATAVVDHRSFDGRDSFDAALAAEIDRFA
PDLVVLAGFMRILTPEFVRRYEGRLLNIHPSLLPSFKGIRTHQQALDAGVALHGATVHFVIPELDSGAIVAQGAVPVRAG
DDAAALAQRVLAVEHVLYPRAVRWFVEGRLRLEGDRAIVAPEEARWIFADQPQTETSEGV

Sequences:

>Translated_220_residues
MKKLVILISGRGSNMEAIVRACTHERWPAEVAAVIANRPDAAGLAFAASHGIATAVVDHRSFDGRDSFDAALAAEIDRFA
PDLVVLAGFMRILTPEFVRRYEGRLLNIHPSLLPSFKGIRTHQQALDAGVALHGATVHFVIPELDSGAIVAQGAVPVRAG
DDAAALAQRVLAVEHVLYPRAVRWFVEGRLRLEGDRAIVAPEEARWIFADQPQTETSEGV
>Mature_220_residues
MKKLVILISGRGSNMEAIVRACTHERWPAEVAAVIANRPDAAGLAFAASHGIATAVVDHRSFDGRDSFDAALAAEIDRFA
PDLVVLAGFMRILTPEFVRRYEGRLLNIHPSLLPSFKGIRTHQQALDAGVALHGATVHFVIPELDSGAIVAQGAVPVRAG
DDAAALAQRVLAVEHVLYPRAVRWFVEGRLRLEGDRAIVAPEEARWIFADQPQTETSEGV

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=190, Percent_Identity=44.2105263157895, Blast_Score=177, Evalue=9e-45,
Organism=Homo sapiens, GI209869995, Length=190, Percent_Identity=44.2105263157895, Blast_Score=177, Evalue=9e-45,
Organism=Homo sapiens, GI209869993, Length=190, Percent_Identity=44.2105263157895, Blast_Score=177, Evalue=9e-45,
Organism=Escherichia coli, GI1788846, Length=205, Percent_Identity=48.780487804878, Blast_Score=201, Evalue=4e-53,
Organism=Escherichia coli, GI1787483, Length=198, Percent_Identity=33.3333333333333, Blast_Score=108, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17567511, Length=180, Percent_Identity=40.5555555555556, Blast_Score=137, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6320616, Length=200, Percent_Identity=29, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24582400, Length=203, Percent_Identity=41.871921182266, Blast_Score=160, Evalue=8e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 23796; Mature: 23796

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLVILISGRGSNMEAIVRACTHERWPAEVAAVIANRPDAAGLAFAASHGIATAVVDHR
CCEEEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEHHHCCCEEEEEECC
SFDGRDSFDAALAAEIDRFAPDLVVLAGFMRILTPEFVRRYEGRLLNIHPSLLPSFKGIR
CCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHCHHHHHHHCCCEEEECHHHCCCCCCHH
THQQALDAGVALHGATVHFVIPELDSGAIVAQGAVPVRAGDDAAALAQRVLAVEHVLYPR
HHHHHHHCCEEEECCEEEEEEECCCCCCEEECCCEEEECCCCHHHHHHHHHHHHHHHHHH
AVRWFVEGRLRLEGDRAIVAPEEARWIFADQPQTETSEGV
HHHHHHCCEEEECCCEEEECCCCCEEEECCCCCCCCCCCC
>Mature Secondary Structure
MKKLVILISGRGSNMEAIVRACTHERWPAEVAAVIANRPDAAGLAFAASHGIATAVVDHR
CCEEEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEHHHCCCEEEEEECC
SFDGRDSFDAALAAEIDRFAPDLVVLAGFMRILTPEFVRRYEGRLLNIHPSLLPSFKGIR
CCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHCHHHHHHHCCCEEEECHHHCCCCCCHH
THQQALDAGVALHGATVHFVIPELDSGAIVAQGAVPVRAGDDAAALAQRVLAVEHVLYPR
HHHHHHHCCEEEECCEEEEEEECCCCCCEEECCCEEEECCCCHHHHHHHHHHHHHHHHHH
AVRWFVEGRLRLEGDRAIVAPEEARWIFADQPQTETSEGV
HHHHHHCCEEEECCCEEEECCCCCEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]