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The map label for this gene is yieH [H]

Identifier: 161523885

GI number: 161523885

Start: 774327

End: 775025

Strand: Direct

Name: yieH [H]

Synonym: Bmul_0705

Alternate gene names: 161523885

Gene position: 774327-775025 (Clockwise)

Preceding gene: 161523884

Following gene: 161523886

Centisome position: 22.45

GC content: 75.11

Gene sequence:

>699_bases
GTGAGCGCGGCCGACGAACGCGCGAGCGTGCTGATCTGCGACTGCGACGGCGTGCTGATCGACAGCGAGGCCGTCGCCGC
CGACGTGCTCGTGCGCGAGCTCGAGGCGCGCTGGCCGGGCGTCGATGCGCGGCCGGCCGTGATGCCGCTGCTCGGGCTGC
GCATCGAGCGCGTGCTGGCCGGCGCGAGCGAAGCGGTCGGCCGCACGCTGTCGGCGGACGACGTCGACGCGATTCGCTGC
GCCGTCGAAGCGGCGGCCGTGCATGCGCCGATGGTCGAGGGCATCGACGCGGCGCTCGCGGCGATCGAGCTGCCGAAGGC
CTGCGCGAGCAACAGCTATCGCGGCTACGTCGAAGCGGCGCTCGCGCGCACGGGCCTCGCGCGCTTCTTCGGCGACCGGC
TGTTCTGCGCGGATGCGGTCGCGCGGCCGAAGCCCGCGCCCGACGTCTATCTCGCGGCCGCGCACGCGCTCGGCGTCGCG
CCCGCGCAGTGCCTCGTCGTCGAGGACAGCGCGACCGGGATCACCGCGGCCGCCGCGGCCGGCATGACGGTACTCGCATT
CGTCGGCGGCGGGCATGCGCCGGCCGCGCAGAGCGACGCGCTGCGCAGCATCGGCGCACGCCACGTATTCGACGACATGC
ACGAGCTGCCCGGCTACGTCGCGCGCTGGCAGGCGACGGGCGCGGTGCTGCCGAACTAG

Upstream 100 bases:

>100_bases
AGCTGTCCGCGGCATCGCTGCTCGCCGTCGCGCCGATCCTGATCGTCGGCTGGATCTCGCAGAAGCAGCTGGTGCGCGGG
CTCACGTTCGGAGCGGTCAA

Downstream 100 bases:

>100_bases
CAACGATTGCGCTGCGTTCCGTGCGGCGCATCACGAACGAAACGGAGACAGACCATGGCAAGCGTGCTCCTGCGCAACAT
CGCGAAGCGCTACGACGACA

Product: HAD family hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 232; Mature: 231

Protein sequence:

>232_residues
MSAADERASVLICDCDGVLIDSEAVAADVLVRELEARWPGVDARPAVMPLLGLRIERVLAGASEAVGRTLSADDVDAIRC
AVEAAAVHAPMVEGIDAALAAIELPKACASNSYRGYVEAALARTGLARFFGDRLFCADAVARPKPAPDVYLAAAHALGVA
PAQCLVVEDSATGITAAAAAGMTVLAFVGGGHAPAAQSDALRSIGARHVFDDMHELPGYVARWQATGAVLPN

Sequences:

>Translated_232_residues
MSAADERASVLICDCDGVLIDSEAVAADVLVRELEARWPGVDARPAVMPLLGLRIERVLAGASEAVGRTLSADDVDAIRC
AVEAAAVHAPMVEGIDAALAAIELPKACASNSYRGYVEAALARTGLARFFGDRLFCADAVARPKPAPDVYLAAAHALGVA
PAQCLVVEDSATGITAAAAAGMTVLAFVGGGHAPAAQSDALRSIGARHVFDDMHELPGYVARWQATGAVLPN
>Mature_231_residues
SAADERASVLICDCDGVLIDSEAVAADVLVRELEARWPGVDARPAVMPLLGLRIERVLAGASEAVGRTLSADDVDAIRCA
VEAAAVHAPMVEGIDAALAAIELPKACASNSYRGYVEAALARTGLARFFGDRLFCADAVARPKPAPDVYLAAAHALGVAP
AQCLVVEDSATGITAAAAAGMTVLAFVGGGHAPAAQSDALRSIGARHVFDDMHELPGYVARWQATGAVLPN

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates phosphoenolpyruvate and AMP [H]

COG id: COG0637

COG function: function code R; Predicted phosphatase/phosphohexomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1790151, Length=188, Percent_Identity=26.5957446808511, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1789046, Length=182, Percent_Identity=32.4175824175824, Blast_Score=71, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 23782; Mature: 23651

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAADERASVLICDCDGVLIDSEAVAADVLVRELEARWPGVDARPAVMPLLGLRIERVLA
CCCCCCCCEEEEECCCCEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
GASEAVGRTLSADDVDAIRCAVEAAAVHAPMVEGIDAALAAIELPKACASNSYRGYVEAA
CCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHH
LARTGLARFFGDRLFCADAVARPKPAPDVYLAAAHALGVAPAQCLVVEDSATGITAAAAA
HHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHH
GMTVLAFVGGGHAPAAQSDALRSIGARHVFDDMHELPGYVARWQATGAVLPN
HHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SAADERASVLICDCDGVLIDSEAVAADVLVRELEARWPGVDARPAVMPLLGLRIERVLA
CCCCCCCEEEEECCCCEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
GASEAVGRTLSADDVDAIRCAVEAAAVHAPMVEGIDAALAAIELPKACASNSYRGYVEAA
CCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHH
LARTGLARFFGDRLFCADAVARPKPAPDVYLAAAHALGVAPAQCLVVEDSATGITAAAAA
HHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHH
GMTVLAFVGGGHAPAAQSDALRSIGARHVFDDMHELPGYVARWQATGAVLPN
HHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]