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The map label for this gene is mexA [H]

Identifier: 161523868

GI number: 161523868

Start: 752494

End: 753789

Strand: Direct

Name: mexA [H]

Synonym: Bmul_0688

Alternate gene names: 161523868

Gene position: 752494-753789 (Clockwise)

Preceding gene: 161523865

Following gene: 161523869

Centisome position: 21.82

GC content: 70.14

Gene sequence:

>1296_bases
ATGCGCGTCGAACGGGTTCCATACCGCTTACTCACTGTCGCGACGGCCGCCGTTTTCCTGGCCGCGTGCGGGAAAAAAGA
ATCGGCACCGCCGCCGCAAACGCCGGAAGTCGGCGTCGTCACGGTCCAGCCGCAGGCCGTACCGGTCTTCACCGAACTGC
CTGGCCGCACCAGCGCGTTCCTCGTCGCGCAGGTCCGCGCGCGGGTCGACGGCATCGTGCTGCGCCGTGAATTCACCGAA
GGCAGCGACGTCAAGGCCGGTCAGCGCCTCTACAAGATCGACCCGGCACCGTATATCGCCGCGCTGAACAGCGCGAAGGC
GACGCTCGCGAAGGCGCAGGCGAACCTCGCCACGCAGAACGCGCTCGTCGCGCGCTACAAGGTGCTGGTGGCCGCGAACG
CGGTCAGCAAGCAGGACTACGACAACGCGGTGGCCGCGCAAGGGCAGGCCGCCGCCGACGTCGCGGCCGGCAAGGCGTCG
GTCGAGACCGCGCAGATCAACCTCGGCTACACGGACGTCGTATCGCCGATCACCGGCCGCGTCGGCATCTCGCAGGTGAC
GCCGGGCGCGTACGTGCAGGCGAGCCAGGCGACGCTGATGTCGACCGTGCAGCAGCTCGACCCGGTCTACGTCGACCTCA
CGCAATCGAGCCTCGAGGGGCTGAAGCTGCGTCAGGACGTGCAGAGCGGCCGTCTGAAGACGACGGGCCCCGGTGCCGCG
AAGGTGTCGCTGATCCTGGAGGACGGCCGCACCTATTCGCAGCCCGGCAAGCTGCAGTTCTCCGACGTGACGGTCGACCA
GACGACCGGCTCGGTCACGATCCGTGCGGTGTTCCCGAACCCGGGCAAGGTCCTGCTGCCGGGGATGTTCGTGCGCGCGC
GCATCGAGGAAGGCGTGAACGACAACGCGTATCTGGTGCCGCAGATCGGCGTCACGCACGACCAGAAGGGCCAGGCGGTC
GCGATGGTCGTCAACGCGAACAACAAGGTCGAGCCGCGCACGCTGACCGCAACGCGCATCGAAGGTCAGAACTGGGTCGT
CGAAAGCGGGCTGCAGCCCGGCGATCGCGTGATCGTGCAGGGTGTCGACAAGGTGCGTCCGGGCGCGACCGTGAAGGCCG
TTCCGGCGCAGCTCGCGTCGGCGCCGAACGACGCGTCGGGTGCCGCCGCGCCGGCCGCCGCCGCACCGGCTTCGGCCGCC
GCGGCATCGGCCGCCGCAGCATCGAGTGCCGGCGCGTCGGGCGCGCCGGCTAGCGCCGCCGCGGGGTCGTCCGCCGCCGC
TTCGGGCGCGCAATAA

Upstream 100 bases:

>100_bases
AGTAATTCAGGTGTTCGATCTGCGCCGCGAGGCGCATCCGTGCCGTGCTCCGGGGTCGGGCGCCGCACTCATTCCATTGG
TTATACACAGAGGTCGCTCC

Downstream 100 bases:

>100_bases
CAGGGAGCCTGTTTCATGGCAAAGTTTTTTATCGATCGCCCGATCTTCGCGTGGGTGATCGCCATCATCCTGATGCTGGC
CGGGGTTGCGGCGATCTTCA

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MRVERVPYRLLTVATAAVFLAACGKKESAPPPQTPEVGVVTVQPQAVPVFTELPGRTSAFLVAQVRARVDGIVLRREFTE
GSDVKAGQRLYKIDPAPYIAALNSAKATLAKAQANLATQNALVARYKVLVAANAVSKQDYDNAVAAQGQAAADVAAGKAS
VETAQINLGYTDVVSPITGRVGISQVTPGAYVQASQATLMSTVQQLDPVYVDLTQSSLEGLKLRQDVQSGRLKTTGPGAA
KVSLILEDGRTYSQPGKLQFSDVTVDQTTGSVTIRAVFPNPGKVLLPGMFVRARIEEGVNDNAYLVPQIGVTHDQKGQAV
AMVVNANNKVEPRTLTATRIEGQNWVVESGLQPGDRVIVQGVDKVRPGATVKAVPAQLASAPNDASGAAAPAAAAPASAA
AASAAAASSAGASGAPASAAAGSSAAASGAQ

Sequences:

>Translated_431_residues
MRVERVPYRLLTVATAAVFLAACGKKESAPPPQTPEVGVVTVQPQAVPVFTELPGRTSAFLVAQVRARVDGIVLRREFTE
GSDVKAGQRLYKIDPAPYIAALNSAKATLAKAQANLATQNALVARYKVLVAANAVSKQDYDNAVAAQGQAAADVAAGKAS
VETAQINLGYTDVVSPITGRVGISQVTPGAYVQASQATLMSTVQQLDPVYVDLTQSSLEGLKLRQDVQSGRLKTTGPGAA
KVSLILEDGRTYSQPGKLQFSDVTVDQTTGSVTIRAVFPNPGKVLLPGMFVRARIEEGVNDNAYLVPQIGVTHDQKGQAV
AMVVNANNKVEPRTLTATRIEGQNWVVESGLQPGDRVIVQGVDKVRPGATVKAVPAQLASAPNDASGAAAPAAAAPASAA
AASAAAASSAGASGAPASAAAGSSAAASGAQ
>Mature_431_residues
MRVERVPYRLLTVATAAVFLAACGKKESAPPPQTPEVGVVTVQPQAVPVFTELPGRTSAFLVAQVRARVDGIVLRREFTE
GSDVKAGQRLYKIDPAPYIAALNSAKATLAKAQANLATQNALVARYKVLVAANAVSKQDYDNAVAAQGQAAADVAAGKAS
VETAQINLGYTDVVSPITGRVGISQVTPGAYVQASQATLMSTVQQLDPVYVDLTQSSLEGLKLRQDVQSGRLKTTGPGAA
KVSLILEDGRTYSQPGKLQFSDVTVDQTTGSVTIRAVFPNPGKVLLPGMFVRARIEEGVNDNAYLVPQIGVTHDQKGQAV
AMVVNANNKVEPRTLTATRIEGQNWVVESGLQPGDRVIVQGVDKVRPGATVKAVPAQLASAPNDASGAAAPAAAAPASAA
AASAAAASSAGASGAPASAAAGSSAAASGAQ

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=386, Percent_Identity=53.3678756476684, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1789665, Length=372, Percent_Identity=50.5376344086022, Blast_Score=339, Evalue=2e-94,
Organism=Escherichia coli, GI1789929, Length=378, Percent_Identity=48.1481481481481, Blast_Score=338, Evalue=3e-94,
Organism=Escherichia coli, GI87082036, Length=367, Percent_Identity=32.1525885558583, Blast_Score=162, Evalue=5e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 44037; Mature: 44037

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVERVPYRLLTVATAAVFLAACGKKESAPPPQTPEVGVVTVQPQAVPVFTELPGRTSAF
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCEEEEECCCCCCHHH
LVAQVRARVDGIVLRREFTEGSDVKAGQRLYKIDPAPYIAALNSAKATLAKAQANLATQN
HHHHHHHHHCEEEEEEECCCCCCCCCCCEEEEECCCCCEEECCCHHHHHHHHHHHHHHHH
ALVARYKVLVAANAVSKQDYDNAVAAQGQAAADVAAGKASVETAQINLGYTDVVSPITGR
HHHHHEEEEEEECHHCCCCCCCHHHCCCCHHHHHHCCCCEEEEEEEECCHHHHHCCCCCC
VGISQVTPGAYVQASQATLMSTVQQLDPVYVDLTQSSLEGLKLRQDVQSGRLKTTGPGAA
CCCCCCCCCCEEEHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHCCCEEECCCCCE
KVSLILEDGRTYSQPGKLQFSDVTVDQTTGSVTIRAVFPNPGKVLLPGMFVRARIEEGVN
EEEEEEECCCCCCCCCCEEEEEEEEECCCCCEEEEEECCCCCCEECCCHHEEHHHHCCCC
DNAYLVPQIGVTHDQKGQAVAMVVNANNKVEPRTLTATRIEGQNWVVESGLQPGDRVIVQ
CCCEEEEECCCCCCCCCCEEEEEEECCCCCCCEEEEEEEECCCCEEEECCCCCCCEEEEE
GVDKVRPGATVKAVPAQLASAPNDASGAAAPAAAAPASAAAASAAAASSAGASGAPASAA
CHHHCCCCCEEEHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCC
AGSSAAASGAQ
CCCCCCCCCCC
>Mature Secondary Structure
MRVERVPYRLLTVATAAVFLAACGKKESAPPPQTPEVGVVTVQPQAVPVFTELPGRTSAF
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCEEEEECCCCCCHHH
LVAQVRARVDGIVLRREFTEGSDVKAGQRLYKIDPAPYIAALNSAKATLAKAQANLATQN
HHHHHHHHHCEEEEEEECCCCCCCCCCCEEEEECCCCCEEECCCHHHHHHHHHHHHHHHH
ALVARYKVLVAANAVSKQDYDNAVAAQGQAAADVAAGKASVETAQINLGYTDVVSPITGR
HHHHHEEEEEEECHHCCCCCCCHHHCCCCHHHHHHCCCCEEEEEEEECCHHHHHCCCCCC
VGISQVTPGAYVQASQATLMSTVQQLDPVYVDLTQSSLEGLKLRQDVQSGRLKTTGPGAA
CCCCCCCCCCEEEHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHCCCEEECCCCCE
KVSLILEDGRTYSQPGKLQFSDVTVDQTTGSVTIRAVFPNPGKVLLPGMFVRARIEEGVN
EEEEEEECCCCCCCCCCEEEEEEEEECCCCCEEEEEECCCCCCEECCCHHEEHHHHCCCC
DNAYLVPQIGVTHDQKGQAVAMVVNANNKVEPRTLTATRIEGQNWVVESGLQPGDRVIVQ
CCCEEEEECCCCCCCCCCEEEEEEECCCCCCCEEEEEEEECCCCEEEECCCCCCCEEEEE
GVDKVRPGATVKAVPAQLASAPNDASGAAAPAAAAPASAAAASAAAASSAGASGAPASAA
CHHHCCCCCEEEHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCC
AGSSAAASGAQ
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]