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The map label for this gene is thcD [H]

Identifier: 161523797

GI number: 161523797

Start: 675586

End: 676821

Strand: Direct

Name: thcD [H]

Synonym: Bmul_0617

Alternate gene names: 161523797

Gene position: 675586-676821 (Clockwise)

Preceding gene: 161523796

Following gene: 161523801

Centisome position: 19.59

GC content: 73.95

Gene sequence:

>1236_bases
ATGACGAACGACCGCGTGATGGCGATCGTCGGCGCGGGCCACGCGGGCGGCCGTGCGGCGCAGGTGCTGCGCGAATACGG
CTGGCGCGGGCGCATCGTGCTGATCGGTGCGGAGCCGCATCTGCCCTACGAACGGCCGCCGCTGTCGAAAGGCGTGCTGA
CCGGCGAGCGCAGTGCCGCGCAATGCGCGCTGCGCGATCGCGACGCCTGGCGTGCGGACGCGATCGAGCCGATCGTCGCG
ACCGTCGAGCGGATCGATCCGGCGGCGCGCGAAATACGCGTGTCGGACGGCCGCGCGTTCGCGTACGACGCGCTGCTGCT
GGCGACCGGCGGCCGCGCGCGGCGCCTCGCGATTCCCGGTGCCGATCTCGACGGCGTGCTCGCCCTGCGCACGCTCGACG
ACGCCGCGGCGCTCGGCGCGCGGCTCGTGCGCGATGCGCGCATCGTGCTGATCGGCGGCGGCTTCATCGGGCTCGAAGTC
GCGGCGTCGGCCCGCCGGCGCGGCTGTCGCGTGACCGTGCTCGACGCCGCGTCGCGCGTGCTCGGCCGCGCGGTGCCGGA
CACGATCGCGGCGCGCGTGCATGCGCTGCACGTTCGGCACGGCGTCGCGATCCGGCTCAATCGCGCGCCGGTCGCGATCG
AGCGCACGGCGGGCGGCGCGCTCGCGGTCGTGCTCGACGACGGCGACACGCTGATCGCCGATACGGTCGTCGTCGGGATC
GGCATCGAGCCGGCCGACGAACTCGCCCGTGACGCGGGGCTCGCGGTCGATCGCGGGATCGTCGTCGACGAAAGGCTCGA
GACGTCGGCGCGCGGCATTTACGCGGCCGGCGACGTCGCGGTGTTTCCTGGCGGCTCGTCGGCACGTCGCGTGCGGCAGG
AGACGTGGCACGGCGCCGAGACGCAGGCGCGCGTCGCCGCGCGCAACATGCTGGGCGCCGACGAACCGTATCGCGACGTG
CGCTGGTTCTGGTCCGATCAGTACGACGCGCAGCTGCAGGTCGTCGGCGAGCCCGCGCTTGGCGAGCGGACCGTCGCGCG
CGTGCTCGACGACGACGCGGAAATCCGCTTCCATTTCGATGCGCAGGCGCGGCTCGTCGCGGCGAGCGGGTTCGGCTGCG
CGCCCACGTGGGTGAAGGAGATGCGGGTCGCGAGGATGCTCGTCGAGCGCGCGGCGGACGTGACGCCCGCCGACGTCGCG
GACACCGGCGTGAAGCTGAAATCGCTGCTGCGCTGA

Upstream 100 bases:

>100_bases
GTGCGCGACGGCCGCGCATTGTCGGCACCCGCATGCGAGCGCTTGCGCACGTATCGCGCGCGGATCGAGGACGGCCGTGT
GCTGATCGAGGTATGACGGC

Downstream 100 bases:

>100_bases
TGTGAACGAGCCGCGGCGCATGCGGCGCCGAGCACAGAGAAGGGAGGGTGCGAGGCCGAAGCGCGCGGCCCCGAGTGTGC
CGCGCGCTTCGGTGTGCGTA

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MTNDRVMAIVGAGHAGGRAAQVLREYGWRGRIVLIGAEPHLPYERPPLSKGVLTGERSAAQCALRDRDAWRADAIEPIVA
TVERIDPAAREIRVSDGRAFAYDALLLATGGRARRLAIPGADLDGVLALRTLDDAAALGARLVRDARIVLIGGGFIGLEV
AASARRRGCRVTVLDAASRVLGRAVPDTIAARVHALHVRHGVAIRLNRAPVAIERTAGGALAVVLDDGDTLIADTVVVGI
GIEPADELARDAGLAVDRGIVVDERLETSARGIYAAGDVAVFPGGSSARRVRQETWHGAETQARVAARNMLGADEPYRDV
RWFWSDQYDAQLQVVGEPALGERTVARVLDDDAEIRFHFDAQARLVAASGFGCAPTWVKEMRVARMLVERAADVTPADVA
DTGVKLKSLLR

Sequences:

>Translated_411_residues
MTNDRVMAIVGAGHAGGRAAQVLREYGWRGRIVLIGAEPHLPYERPPLSKGVLTGERSAAQCALRDRDAWRADAIEPIVA
TVERIDPAAREIRVSDGRAFAYDALLLATGGRARRLAIPGADLDGVLALRTLDDAAALGARLVRDARIVLIGGGFIGLEV
AASARRRGCRVTVLDAASRVLGRAVPDTIAARVHALHVRHGVAIRLNRAPVAIERTAGGALAVVLDDGDTLIADTVVVGI
GIEPADELARDAGLAVDRGIVVDERLETSARGIYAAGDVAVFPGGSSARRVRQETWHGAETQARVAARNMLGADEPYRDV
RWFWSDQYDAQLQVVGEPALGERTVARVLDDDAEIRFHFDAQARLVAASGFGCAPTWVKEMRVARMLVERAADVTPADVA
DTGVKLKSLLR
>Mature_410_residues
TNDRVMAIVGAGHAGGRAAQVLREYGWRGRIVLIGAEPHLPYERPPLSKGVLTGERSAAQCALRDRDAWRADAIEPIVAT
VERIDPAAREIRVSDGRAFAYDALLLATGGRARRLAIPGADLDGVLALRTLDDAAALGARLVRDARIVLIGGGFIGLEVA
ASARRRGCRVTVLDAASRVLGRAVPDTIAARVHALHVRHGVAIRLNRAPVAIERTAGGALAVVLDDGDTLIADTVVVGIG
IEPADELARDAGLAVDRGIVVDERLETSARGIYAAGDVAVFPGGSSARRVRQETWHGAETQARVAARNMLGADEPYRDVR
WFWSDQYDAQLQVVGEPALGERTVARVLDDDAEIRFHFDAQARLVAASGFGCAPTWVKEMRVARMLVERAADVTPADVAD
TGVKLKSLLR

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI226437568, Length=332, Percent_Identity=33.1325301204819, Blast_Score=168, Evalue=7e-42,
Organism=Homo sapiens, GI21389617, Length=332, Percent_Identity=33.1325301204819, Blast_Score=168, Evalue=7e-42,
Organism=Homo sapiens, GI65787454, Length=332, Percent_Identity=33.1325301204819, Blast_Score=168, Evalue=8e-42,
Organism=Homo sapiens, GI4757732, Length=336, Percent_Identity=29.7619047619048, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI22202629, Length=336, Percent_Identity=29.7619047619048, Blast_Score=112, Evalue=8e-25,
Organism=Homo sapiens, GI22202631, Length=169, Percent_Identity=33.1360946745562, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1788892, Length=336, Percent_Identity=38.9880952380952, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1789065, Length=316, Percent_Identity=31.3291139240506, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1789915, Length=194, Percent_Identity=28.8659793814433, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789765, Length=320, Percent_Identity=27.1875, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17559934, Length=330, Percent_Identity=29.6969696969697, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI32564386, Length=342, Percent_Identity=27.4853801169591, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24639257, Length=378, Percent_Identity=28.042328042328, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI281359715, Length=378, Percent_Identity=28.042328042328, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI281359713, Length=378, Percent_Identity=28.042328042328, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24639250, Length=378, Percent_Identity=28.042328042328, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI18543267, Length=378, Percent_Identity=28.042328042328, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24639252, Length=378, Percent_Identity=28.042328042328, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24585130, Length=378, Percent_Identity=24.0740740740741, Blast_Score=132, Evalue=6e-31,
Organism=Drosophila melanogaster, GI28573993, Length=393, Percent_Identity=29.0076335877863, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24581020, Length=390, Percent_Identity=28.7179487179487, Blast_Score=119, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 43886; Mature: 43754

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNDRVMAIVGAGHAGGRAAQVLREYGWRGRIVLIGAEPHLPYERPPLSKGVLTGERSAA
CCCCEEEEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHH
QCALRDRDAWRADAIEPIVATVERIDPAAREIRVSDGRAFAYDALLLATGGRARRLAIPG
HHHHCCCCCCCHHHHHHHHHHHHHCCCCHHEEEECCCCEEEEEEEEEECCCCEEEEECCC
ADLDGVLALRTLDDAAALGARLVRDARIVLIGGGFIGLEVAASARRRGCRVTVLDAASRV
CCCCCEEEEEEHHHHHHHHHHHHCCCEEEEEECCEEEHHHHHHHHHCCCEEEEEHHHHHH
LGRAVPDTIAARVHALHVRHGVAIRLNRAPVAIERTAGGALAVVLDDGDTLIADTVVVGI
HHHCCCHHHHHHHHHEEEECCEEEEECCCCEEEEECCCCEEEEEEECCCEEEEEEEEEEE
GIEPADELARDAGLAVDRGIVVDERLETSARGIYAAGDVAVFPGGSSARRVRQETWHGAE
CCCCHHHHHHHCCCEECCCEEECHHHHHCCCCEEEECCEEEECCCCHHHHHHHHHCCCCC
TQARVAARNMLGADEPYRDVRWFWSDQYDAQLQVVGEPALGERTVARVLDDDAEIRFHFD
HHHHHHHHHHCCCCCCCCHHHEEECCCCCCEEEEECCCCCCHHHHHHHHCCCCEEEEEEC
AQARLVAASGFGCAPTWVKEMRVARMLVERAADVTPADVADTGVKLKSLLR
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHCC
>Mature Secondary Structure 
TNDRVMAIVGAGHAGGRAAQVLREYGWRGRIVLIGAEPHLPYERPPLSKGVLTGERSAA
CCCEEEEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHH
QCALRDRDAWRADAIEPIVATVERIDPAAREIRVSDGRAFAYDALLLATGGRARRLAIPG
HHHHCCCCCCCHHHHHHHHHHHHHCCCCHHEEEECCCCEEEEEEEEEECCCCEEEEECCC
ADLDGVLALRTLDDAAALGARLVRDARIVLIGGGFIGLEVAASARRRGCRVTVLDAASRV
CCCCCEEEEEEHHHHHHHHHHHHCCCEEEEEECCEEEHHHHHHHHHCCCEEEEEHHHHHH
LGRAVPDTIAARVHALHVRHGVAIRLNRAPVAIERTAGGALAVVLDDGDTLIADTVVVGI
HHHCCCHHHHHHHHHEEEECCEEEEECCCCEEEEECCCCEEEEEEECCCEEEEEEEEEEE
GIEPADELARDAGLAVDRGIVVDERLETSARGIYAAGDVAVFPGGSSARRVRQETWHGAE
CCCCHHHHHHHCCCEECCCEEECHHHHHCCCCEEEECCEEEECCCCHHHHHHHHHCCCCC
TQARVAARNMLGADEPYRDVRWFWSDQYDAQLQVVGEPALGERTVARVLDDDAEIRFHFD
HHHHHHHHHHCCCCCCCCHHHEEECCCCCCEEEEECCCCCCHHHHHHHHCCCCEEEEEEC
AQARLVAASGFGCAPTWVKEMRVARMLVERAADVTPADVADTGVKLKSLLR
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]