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The map label for this gene is purU [H]

Identifier: 161523714

GI number: 161523714

Start: 581902

End: 582786

Strand: Direct

Name: purU [H]

Synonym: Bmul_0534

Alternate gene names: 161523714

Gene position: 581902-582786 (Clockwise)

Preceding gene: 161523713

Following gene: 161523717

Centisome position: 16.87

GC content: 64.07

Gene sequence:

>885_bases
ATGTCGACCGATCACAGCTTTATCTTGAAACTGTCGTGCCCCGACCGGCACGGCATCGTCCACGCGGTCTCGGGCTTTCT
GTTCGAGCGCAACAGCAACATTCTCGATTCCGCGCAGTTCGGCGACAGCCGCACCGGCGAGTTCTTCATGCGCGTGCATT
TCGAACAGGACGGCGCGGGCCGGGATGCCGCCTCGGCGCTCGACACGCTGCGCGACGATTTCGCGCCGCTCGCCGAGCAG
TTCGCGATGCGTTGGGAACTGCACGATGCGTCGGTGAAACCGCGCGTCGTGATCATGGTGTCGAAGATCGGCCATTGCCT
GAACGACCTGCTGTTCCGCTATCGCACGGGCCAGCTGCCGATCGAGATTCCGGCGATCGTGTCGAACCACAAGGACTTCT
ATCAGCTTGCGGCGAGCTACGACATCCCGTTCCATCACTTCCCGCTGGTCGGCGGTTCGTCCGACGCCGCGAAGGCCGCG
CAGGAAGCGCGCGTGCTCGACGTGATCGACGAGCATCAGGCCGACCTCGTCGTGCTCGCGCGCTACATGCAGATCCTGTC
GCCGAACATGTGCGAGCGGCTCGCCGGGCGCGCGATCAACATCCATCACTCGTTCCTGCCGAGCTTCAAGGGCGCGAAGC
CTTACTATCAGGCGTTCGACCGCGGCGTGAAGCTGATCGGCGCGACCGCGCACTACGTGACGACGGATCTCGACGAAGGG
CCGATCATCGAGCAGGAGGTCGAGCGTGTCGATCACAGCATGACGCCGGACCAGCTGACCGCGATCGGCCGCGACGTCGA
ATGCGTGACGCTTGCGCGCGCCGTGAAGTGGCACGTCGAGCACCGCATCGTGCTCAACGGTACGAAGACGGTCGTGTTCC
GCTGA

Upstream 100 bases:

>100_bases
GCGGGCCTCGATGCGCTGTTCGCGCCGGCCGCCTGACGCGGCTCGCGGCCGCGGCGCACACGAGCCATTTGACGAATACG
GAAACGAAGGGAGTACGGTC

Downstream 100 bases:

>100_bases
GCGGCCGCTTCGGCACGCGTCGCCCAAACGAGAAACGCCGCGCCCCGACATTCGGTGACGCGGCGTTTTTTTTCGCGTGC
GGCGCGGCGTCAGATCATCC

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MSTDHSFILKLSCPDRHGIVHAVSGFLFERNSNILDSAQFGDSRTGEFFMRVHFEQDGAGRDAASALDTLRDDFAPLAEQ
FAMRWELHDASVKPRVVIMVSKIGHCLNDLLFRYRTGQLPIEIPAIVSNHKDFYQLAASYDIPFHHFPLVGGSSDAAKAA
QEARVLDVIDEHQADLVVLARYMQILSPNMCERLAGRAINIHHSFLPSFKGAKPYYQAFDRGVKLIGATAHYVTTDLDEG
PIIEQEVERVDHSMTPDQLTAIGRDVECVTLARAVKWHVEHRIVLNGTKTVVFR

Sequences:

>Translated_294_residues
MSTDHSFILKLSCPDRHGIVHAVSGFLFERNSNILDSAQFGDSRTGEFFMRVHFEQDGAGRDAASALDTLRDDFAPLAEQ
FAMRWELHDASVKPRVVIMVSKIGHCLNDLLFRYRTGQLPIEIPAIVSNHKDFYQLAASYDIPFHHFPLVGGSSDAAKAA
QEARVLDVIDEHQADLVVLARYMQILSPNMCERLAGRAINIHHSFLPSFKGAKPYYQAFDRGVKLIGATAHYVTTDLDEG
PIIEQEVERVDHSMTPDQLTAIGRDVECVTLARAVKWHVEHRIVLNGTKTVVFR
>Mature_293_residues
STDHSFILKLSCPDRHGIVHAVSGFLFERNSNILDSAQFGDSRTGEFFMRVHFEQDGAGRDAASALDTLRDDFAPLAEQF
AMRWELHDASVKPRVVIMVSKIGHCLNDLLFRYRTGQLPIEIPAIVSNHKDFYQLAASYDIPFHHFPLVGGSSDAAKAAQ
EARVLDVIDEHQADLVVLARYMQILSPNMCERLAGRAINIHHSFLPSFKGAKPYYQAFDRGVKLIGATAHYVTTDLDEGP
IIEQEVERVDHSMTPDQLTAIGRDVECVTLARAVKWHVEHRIVLNGTKTVVFR

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=182, Percent_Identity=31.3186813186813, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI209869995, Length=182, Percent_Identity=31.3186813186813, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI209869993, Length=182, Percent_Identity=31.3186813186813, Blast_Score=81, Evalue=9e-16,
Organism=Escherichia coli, GI1787483, Length=286, Percent_Identity=40.5594405594406, Blast_Score=218, Evalue=3e-58,
Organism=Escherichia coli, GI1788846, Length=191, Percent_Identity=28.7958115183246, Blast_Score=87, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17567511, Length=190, Percent_Identity=30.5263157894737, Blast_Score=91, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32980; Mature: 32849

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTDHSFILKLSCPDRHGIVHAVSGFLFERNSNILDSAQFGDSRTGEFFMRVHFEQDGAG
CCCCCEEEEEEECCCCCCHHHHHHHHHEECCCCCCCHHHCCCCCCCEEEEEEEECCCCCC
RDAASALDTLRDDFAPLAEQFAMRWELHDASVKPRVVIMVSKIGHCLNDLLFRYRTGQLP
CHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCC
IEIPAIVSNHKDFYQLAASYDIPFHHFPLVGGSSDAAKAAQEARVLDVIDEHQADLVVLA
EEEHHHHCCCHHHHHHHHHCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHH
RYMQILSPNMCERLAGRAINIHHSFLPSFKGAKPYYQAFDRGVKLIGATAHYVTTDLDEG
HHHHHHCHHHHHHHCCCEEEEEHHHCCCCCCCCHHHHHHHCCCEEEECCHHEEEECCCCC
PIIEQEVERVDHSMTPDQLTAIGRDVECVTLARAVKWHVEHRIVLNGTKTVVFR
CCCHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHCEEEEEECCEEEEEC
>Mature Secondary Structure 
STDHSFILKLSCPDRHGIVHAVSGFLFERNSNILDSAQFGDSRTGEFFMRVHFEQDGAG
CCCCEEEEEEECCCCCCHHHHHHHHHEECCCCCCCHHHCCCCCCCEEEEEEEECCCCCC
RDAASALDTLRDDFAPLAEQFAMRWELHDASVKPRVVIMVSKIGHCLNDLLFRYRTGQLP
CHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCC
IEIPAIVSNHKDFYQLAASYDIPFHHFPLVGGSSDAAKAAQEARVLDVIDEHQADLVVLA
EEEHHHHCCCHHHHHHHHHCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHH
RYMQILSPNMCERLAGRAINIHHSFLPSFKGAKPYYQAFDRGVKLIGATAHYVTTDLDEG
HHHHHHCHHHHHHHCCCEEEEEHHHCCCCCCCCHHHHHHHCCCEEEECCHHEEEECCCCC
PIIEQEVERVDHSMTPDQLTAIGRDVECVTLARAVKWHVEHRIVLNGTKTVVFR
CCCHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHCEEEEEECCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]